Tau-1 Antibody, clone PC1C6, Alexa Fluor® 488 Conjugate | EMD Millipore MAB3420A4 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Tau-1 Antibody, clone PC1C6, Alexa Fluor® 488 Conjugate

catalog :

MAB3420A4

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

AF488

clone name :

PC1C6

reactivity :

human, mouse, bovine

application :

immunohistochemistry, immunocytochemistry

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100; loading ...; fig 2a	Wang J, Xu S, Duan J, Yi L, Guo Y, Shi Y, et al. Invasion of white matter tracts by glioma stem cells is regulated by a NOTCH1-SOX2 positive-feedback loop. Nat Neurosci. 2019;22:91-105 pubmed publisher
immunocytochemistry; mouse; 1:100; fig 1	Schedin Weiss S, Caesar I, Winblad B, Blom H, Tjernberg L. Super-resolution microscopy reveals ?-secretase at both sides of the neuronal synapse. Acta Neuropathol Commun. 2016;4:29 pubmed publisher
	Schedin Weiss S, Inoue M, Hromadkova L, Teranishi Y, Yamamoto N, Wiehager B, et al. Monoamine oxidase B is elevated in Alzheimer disease neurons, is associated with ?-secretase and regulates neuronal amyloid ?-peptide levels. Alzheimers Res Ther. 2017;9:57 pubmed publisher

product information

Catalog Number :

MAB3420A4

Subcategory :

Neuroscience

Product Name :

Anti-Tau-1 Antibody, clone PC1C6, Alexa Fluor® 488 Conjugate

Product Type :

Antibodies

Gene ID :

P10636

Host Name :

Mouse

Antigen :

Tau-1

Conjugate :

AlexaFluor®488

Isotype :

IgG2a

Product Description :

Anti-Tau-1 Antibody, clone PC1C6, Alexa Fluor® 488 Conjugate

Cross Reactivity :

Rat;Mouse;Bovine

Background :

Tau, a microtubulebinding protein which serves to stabilize microtubules in growing axons, is found to be hyperphosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by glycogen synthase kinase 3 (GSK 3). Cellular and subcellular localization: In situ, anti-tau-1 has a stringent specificity for the axons of neurons. The antibody does not stain the cell bodies or dendrites of neurons, nor does it stain any other cell type (4). However, this in vivo intracellular specificity is not maintained in culture: anti-tau-1 stains the axon, cell bodies, and dendrites of rat hippocampal neurons grown in culture (5). The specificity of anti-tau-1 was originally thought to represent the restricted expression of tau to axons. Later studies revealed that this specificity is dependant on the state of phosphorylation. In dephosphorylated samples (samples treated with alkaline phosphatase) anti-tau-1 stains astrocytes, perineuronal glial cells, and the axons, cell bodies and dendrites of neurons, while in untreated samples, anti-tau-1 stains only axons (6). (The epitope recognized by anti-tau-1 is probably at or near a phosphorylated site.)

ALT Names :

Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau

Immunogen :

Purified denatured bovine microtubule associated proteins.

Specificity :

This antibody binds to all known electrophoretic species of tau in human, rat, and bovine brain (one-dimensional SDS-PAGE).

Package Size :

100 µL

Uses :

Immunocytochemistry;Immunohistochemistry

Storage :

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.