Tau-1 Antibody, clone PC1C6 | EMD Millipore MAB3420 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Tau-1 Antibody, clone PC1C6

catalog :

MAB3420

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PC1C6

The same clone is also sold as:

Absolute Antibody: Ab01120-2.0, Ab01120-23.0, Ab01120-2.3, Ab01120-1.32

reactivity :

Neisseria gonorrhoeae, human, mouse, rat, fruit fly

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation

citations: 143

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100; fig 10a western blot; human; loading ...; fig 6f western blot knockout validation; mouse; loading ...; fig 6f	Ercan E, Eid S, Weber C, Kowalski A, Bichmann M, Behrendt A, et al. A validated antibody panel for the characterization of tau post-translational modifications. Mol Neurodegener. 2017;12:87 pubmed publisher
western blot knockout validation; mouse; loading ...; fig 9i western blot; rat; loading ...; fig 6d	Villarroel Campos D, Henríquez D, Bodaleo F, Oguchi M, Bronfman F, Fukuda M, et al. Rab35 Functions in Axon Elongation Are Regulated by P53-Related Protein Kinase in a Mechanism That Involves Rab35 Protein Degradation and the Microtubule-Associated Protein 1B. J Neurosci. 2016;36:7298-313 pubmed publisher
western blot knockout validation; mouse; 1:1000; fig 2	Petry F, Pelletier J, Bretteville A, Morin F, Calon F, Hébert S, et al. Specificity of anti-tau antibodies when analyzing mice models of Alzheimer's disease: problems and solutions. PLoS ONE. 2014;9:e94251 pubmed publisher
ELISA; human; loading ...; fig 5a	Ercan Herbst E, Ehrig J, Schöndorf D, Behrendt A, Klaus B, Gomez Ramos B, et al. A post-translational modification signature defines changes in soluble tau correlating with oligomerization in early stage Alzheimer's disease brain. Acta Neuropathol Commun. 2019;7:192 pubmed publisher
immunocytochemistry; rat; loading ...; fig 1d	Farías G, Fréal A, Tortosa E, Stucchi R, Pan X, Portegies S, et al. Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity. Neuron. 2019;102:184-201.e8 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 2c western blot; mouse; loading ...; fig 2a	Kubo A, Misonou H, Matsuyama M, Nomori A, Wada Kakuda S, Takashima A, et al. Distribution of endogenous normal tau in the mouse brain. J Comp Neurol. 2019;527:985-998 pubmed publisher
immunocytochemistry; rat; 1:200; loading ...; fig s4	Wang N, Dhumale P, Chiang J, Püschel A. The Sema3A receptor Plexin-A1 suppresses supernumerary axons through Rap1 GTPases. Sci Rep. 2018;8:15647 pubmed publisher
immunocytochemistry; mouse; loading ...; fig s5b	Jacko M, Weyn Vanhentenryck S, Smerdon J, Yan R, Feng H, Williams D, et al. Rbfox Splicing Factors Promote Neuronal Maturation and Axon Initial Segment Assembly. Neuron. 2018;97:853-868.e6 pubmed publisher
immunocytochemistry; mouse; 1:1000; loading ...; fig 1e	Yu J, Chen M, Huang H, Zhu J, Song H, Zhu J, et al. Dynamic m6A modification regulates local translation of mRNA in axons. Nucleic Acids Res. 2018;46:1412-1423 pubmed publisher
immunocytochemistry; mouse; 1:200; loading ...; fig 1a western blot; mouse; 1:5000; loading ...; fig 4b	Tseng J, Xie L, Song S, Xie Y, Allen L, Ajit D, et al. The Deacetylase HDAC6 Mediates Endogenous Neuritic Tau Pathology. Cell Rep. 2017;20:2169-2183 pubmed publisher
immunocytochemistry; mouse; 1:2000; fig 7g immunohistochemistry; mouse; 1:2000; loading ...; fig 5c	Takano T, Wu M, Nakamuta S, Naoki H, Ishizawa N, Namba T, et al. Discovery of long-range inhibitory signaling to ensure single axon formation. Nat Commun. 2017;8:33 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 6b	Barbiero I, Peroni D, Tramarin M, Chandola C, Rusconi L, Landsberger N, et al. The neurosteroid pregnenolone reverts microtubule derangement induced by the loss of a functional CDKL5-IQGAP1 complex. Hum Mol Genet. 2017;26:3520-3530 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig 1p	Savier E, Eglen S, BathÃ©lÃ©my A, Perraut M, Pfrieger F, Lemke G, et al. A molecular mechanism for the topographic alignment of convergent neural maps. elife. 2017;6: pubmed publisher
western blot; human; 1:1000; fig 1a	Trzeciakiewicz H, Tseng J, Wander C, Madden V, Tripathy A, Yuan C, et al. A Dual Pathogenic Mechanism Links Tau Acetylation to Sporadic Tauopathy. Sci Rep. 2017;7:44102 pubmed publisher
immunohistochemistry; mouse; loading ...; fig s1f	Niu Y, Dai Z, Liu W, Zhang C, Yang Y, Guo Z, et al. Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory. elife. 2017;6: pubmed publisher
immunocytochemistry; rat; 1:800; loading ...; fig 4d	Gilbert J, Man H. The X-Linked Autism Protein KIAA2022/KIDLIA Regulates Neurite Outgrowth via N-Cadherin and ?-Catenin Signaling. Eneuro. 2016;3: pubmed
immunocytochemistry; rat; 1:600; loading ...; fig 2a	Oksdath M, Guil A, Grassi D, Sosa L, Quiroga S. The Motor KIF5C Links the Requirements of Stable Microtubules and IGF-1 Receptor Membrane Insertion for Neuronal Polarization. Mol Neurobiol. 2017;54:6085-6096 pubmed publisher
immunohistochemistry; mouse; fig 8	Hamada N, Ito H, Nishijo T, Iwamoto I, Morishita R, Tabata H, et al. Essential role of the nuclear isoform of RBFOX1, a candidate gene for autism spectrum disorders, in the brain development. Sci Rep. 2016;6:30805 pubmed publisher
western blot; rat; 1:2000; fig 8	CARTAGENA C, Mountney A, Hwang H, Swiercz A, Rammelkamp Z, Boutte A, et al. Subacute Changes in Cleavage Processing of Amyloid Precursor Protein and Tau following Penetrating Traumatic Brain Injury. PLoS ONE. 2016;11:e0158576 pubmed publisher
immunocytochemistry; mouse; fig 1	Bodaleo F, Montenegro Venegas C, HenrÃquez D, Court F, Gonzalez Billault C. Microtubule-associated protein 1B (MAP1B)-deficient neurons show structural presynaptic deficiencies in vitro and altered presynaptic physiology. Sci Rep. 2016;6:30069 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 1a	Diez H, Benitez M, Fernandez S, Torres Aleman I, Garrido J, Wandosell F. Class I PI3-kinase or Akt inhibition do not impair axonal polarization, but slow down axonal elongation. Biochim Biophys Acta. 2016;1863:2574-2583 pubmed publisher
immunocytochemistry; mouse; 1:1000; fig s5b western blot; mouse; 1:1000; fig 7b	Itoh Y, Higuchi M, Oishi K, Kishi Y, Okazaki T, Sakai H, et al. PDK1-Akt pathway regulates radial neuronal migration and microtubules in the developing mouse neocortex. Proc Natl Acad Sci U S A. 2016;113:E2955-64 pubmed publisher
western blot; fruit fly ; fig 3	Dourlen P, Fernandez Gomez F, Dupont C, Grenier Boley B, Bellenguez C, Obriot H, et al. Functional screening of Alzheimer risk loci identifies PTK2B as an in vivo modulator and early marker of Tau pathology. Mol Psychiatry. 2017;22:874-883 pubmed publisher
immunohistochemistry; Neisseria gonorrhoeae; 1:500; fig 5	Shah B, Lutter D, Bochenek M, Kato K, Tsytsyura Y, Glyvuk N, et al. C3G/Rapgef1 Is Required in Multipolar Neurons for the Transition to a Bipolar Morphology during Cortical Development. PLoS ONE. 2016;11:e0154174 pubmed publisher
immunocytochemistry; human; fig s4	Kim E, Jeon C, Lee S, Hwang I, Chung T. Robust Type-specific Hemisynapses Induced by Artificial Dendrites. Sci Rep. 2016;6:24210 pubmed publisher
immunocytochemistry; rat; 1:1000; fig 2	Kos A, Wanke K, Gioio A, Martens G, Kaplan B, Aschrafi A. Monitoring mRNA Translation in Neuronal Processes Using Fluorescent Non-Canonical Amino Acid Tagging. J Histochem Cytochem. 2016;64:323-33 pubmed publisher
western blot; mouse; fig 8	Winston C, NoÃ«l A, Neustadtl A, Parsadanian M, Barton D, Chellappa D, et al. Dendritic Spine Loss and Chronic White Matter Inflammation in a Mouse Model of Highly Repetitive Head Trauma. Am J Pathol. 2016;186:552-67 pubmed publisher
immunocytochemistry; mouse; fig 4	Nawaz M, Giarda E, Bedogni F, La Montanara P, Ricciardi S, Ciceri D, et al. CDKL5 and Shootin1 Interact and Concur in Regulating Neuronal Polarization. PLoS ONE. 2016;11:e0148634 pubmed publisher
immunocytochemistry; rat; fig 7a	Yau K, SchÃ¤tzle P, Tortosa E, PagÃ¨s S, Holtmaat A, Kapitein L, et al. Dendrites In Vitro and In Vivo Contain Microtubules of Opposite Polarity and Axon Formation Correlates with Uniform Plus-End-Out Microtubule Orientation. J Neurosci. 2016;36:1071-85 pubmed publisher
western blot; mouse; 1:1000; tbl 1	El Khoury N, Gratuze M, Petry F, Papon M, Julien C, Marcouiller F, et al. Hypothermia mediates age-dependent increase of tau phosphorylation in db/db mice. Neurobiol Dis. 2016;88:55-65 pubmed publisher
western blot; mouse; fig 2a	Parsi S, Smith P, Goupil C, Dorval V, HÃ©bert S. Preclinical Evaluation of miR-15/107 Family Members as Multifactorial Drug Targets for Alzheimer's Disease. Mol Ther Nucleic Acids. 2015;4:e256 pubmed publisher
immunocytochemistry; mouse; 1:1000	Simpson M, Venkatesh I, Callif B, Thiel L, Coley D, Winsor K, et al. The tumor suppressor HHEX inhibits axon growth when prematurely expressed in developing central nervous system neurons. Mol Cell Neurosci. 2015;68:272-83 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000; fig 1	Laclef C, Anselme I, Besse L, Catala M, Palmyre A, Baas D, et al. The role of primary cilia in corpus callosum formation is mediated by production of the Gli3 repressor. Hum Mol Genet. 2015;24:4997-5014 pubmed publisher
western blot; human	De Zio D, Molinari F, Rizza S, Gatta L, Ciotti M, Salvatore A, et al. Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth. Cell Mol Life Sci. 2015;72:4173-91 pubmed publisher
immunocytochemistry; rat; 1:500; fig s2	Deglincerti A, Liu Y, Colak D, Hengst U, Xu G, Jaffrey S. Coupled local translation and degradation regulate growth cone collapse. Nat Commun. 2015;6:6888 pubmed publisher
immunohistochemistry; mouse; 1:50	Lee H, Park J, Seo I, Park S, Kim S. Improved application of the electrophoretic tissue clearing technology, CLARITY, to intact solid organs including brain, pancreas, liver, kidney, lung, and intestine. BMC Dev Biol. 2014;14:48 pubmed publisher
immunocytochemistry; rat; 1:1000; fig s4	Goyal U, Renvoisé B, Chang J, Blackstone C. Spastin-interacting protein NA14/SSNA1 functions in cytokinesis and axon development. PLoS ONE. 2014;9:e112428 pubmed publisher
immunohistochemistry; mouse; 1:1000	Helgager J, Huang Y, McNamara J. Brain-derived neurotrophic factor but not vesicular zinc promotes TrkB activation within mossy fibers of mouse hippocampus in vivo. J Comp Neurol. 2014;522:3885-99 pubmed publisher
immunocytochemistry; rat; 1:1000	Ishikawa M, Shiota J, Ishibashi Y, Hakamata T, Shoji S, Fukuchi M, et al. Cellular localization and dendritic function of rat isoforms of the SRF coactivator MKL1 in cortical neurons. Neuroreport. 2014;25:585-92 pubmed publisher
immunohistochemistry; mouse	Kudo T, Konno K, Uchigashima M, Yanagawa Y, Sora I, Minami M, et al. GABAergic neurons in the ventral tegmental area receive dual GABA/enkephalin-mediated inhibitory inputs from the bed nucleus of the stria terminalis. Eur J Neurosci. 2014;39:1796-809 pubmed publisher
immunohistochemistry; mouse; fig 9	Le Bras B, Fréal A, Czarnecki A, Legendre P, Bullier E, Komada M, et al. In vivo assembly of the axon initial segment in motor neurons. Brain Struct Funct. 2014;219:1433-50 pubmed publisher
western blot; human; 1:10000	Park Y, Ko J, Jang Y, Kwon Y. Activation of AMP-activated protein kinase alleviates homocysteine-mediated neurotoxicity in SH-SY5Y cells. Neurochem Res. 2013;38:1561-71 pubmed publisher
western blot; mouse; loading ...; fig s9	Wang I, Allen M, Goffin D, Zhu X, Fairless A, Brodkin E, et al. Loss of CDKL5 disrupts kinome profile and event-related potentials leading to autistic-like phenotypes in mice. Proc Natl Acad Sci U S A. 2012;109:21516-21 pubmed publisher
immunohistochemistry; mouse; 1:200	Favero C, Henshaw R, Grimsley Myers C, Shrestha A, Beier D, Dwyer N. Mutation of the BiP/GRP78 gene causes axon outgrowth and fasciculation defects in the thalamocortical connections of the mammalian forebrain. J Comp Neurol. 2013;521:677-96 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:2000	An S, Tsai C, Ronecker J, Bayly A, Herzog E. Spatiotemporal distribution of vasoactive intestinal polypeptide receptor 2 in mouse suprachiasmatic nucleus. J Comp Neurol. 2012;520:2730-41 pubmed publisher
immunohistochemistry; fruit fly	Osterloh J, Yang J, Rooney T, Fox A, Adalbert R, Powell E, et al. dSarm/Sarm1 is required for activation of an injury-induced axon death pathway. Science. 2012;337:481-4 pubmed publisher
immunocytochemistry; rat	Sia G, Beique J, Rumbaugh G, Cho R, Worley P, Huganir R. Interaction of the N-terminal domain of the AMPA receptor GluR4 subunit with the neuronal pentraxin NP1 mediates GluR4 synaptic recruitment. Neuron. 2007;55:87-102 pubmed
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	Guo J, Otis J, Suciu S, Catalano C, Xing L, Constable S, et al. Primary Cilia Signaling Promotes Axonal Tract Development and Is Disrupted in Joubert Syndrome-Related Disorders Models. Dev Cell. 2019;51:759-774.e5 pubmed publisher
	Brosig A, Fuchs J, Ipek F, Kroon C, Schrötter S, Vadhvani M, et al. The Axonal Membrane Protein PRG2 Inhibits PTEN and Directs Growth to Branches. Cell Rep. 2019;29:2028-2040.e8 pubmed publisher
	Dupraz S, Hilton B, Husch A, Santos T, Coles C, Stern S, et al. RhoA Controls Axon Extension Independent of Specification in the Developing Brain. Curr Biol. 2019;29:3874-3886.e9 pubmed publisher
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product information

Catalog Number :

MAB3420

Subcategory :

Neuroscience

Product Name :

Anti-Tau-1 Antibody, clone PC1C6

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P10636

Host Name :

Mouse

Antigen :

Tau-1

Clone :

PC1C6

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Tau-1 Antibody, clone PC1C6

Cross Reactivity :

Bovine;Human;Rat

Background :

Tau, a microtubulebinding protein which serves to stabilize microtubules in growing axons, is found to be hyperphosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by glycogen synthase kinase 3 (GSK 3). Cellular and subcellular localization: In situ, anti-tau-1 has a stringent specificity for the axons of neurons. The antibody does not stain the cell bodies or dendrites of neurons, nor does it stain any other cell type (4). However, this in vivo intracellular specificity is not maintained in culture: anti-tau-1 stains the axon, cell bodies, and dendrites of rat hippocampal neurons grown in culture (5). The specificity of anti-tau-1 was originally thought to represent the restricted expression of tau to axons. Later studies revealed that this specificity is dependant on the state of phosphorylation. In dephosphorylated samples (samples treated with alkaline phosphatase) anti-tau-1 stains astrocytes, perineuronal glial cells, and the axons, cell bodies and dendrites of neurons, while in untreated samples, anti-tau-1 stains only axons (6). (The epitope recognized by anti-tau-1 is probably at or near a phosphorylated site.)

Immunogen :

Purified denatured bovine microtubule associated proteins.

Specificity :

Binds to all known electrophoretic species of tau in human, rat and bovine brain (one-dimensional SDS-PAGE). However there is some unphosphorylated bias with clone PC1C6 as it seem to recognize only dephosphorylated serine sites at 195, 198, 199, and 202 {Szendrei, et al 1993; http://www.ncbi.nlm.nih.gov/entrez/query.fcgi-cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7680727}. Also see Billingsley & Kincaid, 1997 Biochem J 323:577-591 for additional mapping information on PC1C6.

Package Size :

100 μg

Uses :

Immunohistochemistry;Western Blotting

Storage :

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.