Cytokeratin AE1/AE3 Antibody, recognizes acidic & basic cytokeratins, clone AE1/AE3 | EMD Millipore MAB3412 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Cytokeratin AE1/AE3 Antibody, recognizes acidic & basic cytokeratins, clone AE1/AE3

catalog :

MAB3412

quantity :

500 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AE1/AE3

The same clone is also sold as:

Novus Biologicals: NBP2-33200, NBP2-29429
US Biological: 138538
OriGene: TA190321
Dako: M351501-2
Leica Biosystems: AE1/AE3-L-CE
Invitrogen: 50-9003-82, 53-9003-82, MA5-13156, MA1-82041, 41-9003-80, 50-9003-80, 42-9003-80, 53-9003-80, 42-9003-82, 41-9003-82
BioLegend: 914202, 914201

reactivity :

human, mouse, domestic sheep

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

citations: 55

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:300; fig 3h	Wang W, Huang Y, Wang C, Hong J, Ma C, Lin N, et al. Intra-articular nodular fasciitis: a rare lesion case report and an updated review of the literature. BMC Musculoskelet Disord. 2019;20:5 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 4b	Cao P, Aoki Y, Badri L, Walker N, Manning C, Lagstein A, et al. Autocrine lysophosphatidic acid signaling activates ?-catenin and promotes lung allograft fibrosis. J Clin Invest. 2017;127:1517-1530 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100	Wilson R, Buckberry S, Spronk F, Laurence J, Leemaqz S, O Leary S, et al. Vitamin D Receptor Gene Ablation in the Conceptus Has Limited Effects on Placental Morphology, Function and Pregnancy Outcome. PLoS ONE. 2015;10:e0131287 pubmed publisher
immunohistochemistry; human; 1:200	LÃ¶nnqvist S, Rakar J, Briheim K, Kratz G. Biodegradable Gelatin Microcarriers Facilitate Re-Epithelialization of Human Cutaneous Wounds - An In Vitro Study in Human Skin. PLoS ONE. 2015;10:e0128093 pubmed publisher
ELISA; mouse; 1:400	Rakar J, Krammer M, Kratz G. Human melanocytes mitigate keratinocyte-dependent contraction in an in vitro collagen contraction assay. Burns. 2015;41:1035-42 pubmed publisher
immunohistochemistry - paraffin section; domestic sheep; 1:200	Miki Y, Hamada K, Yoshino T, Miyatani K, Takahashi K. Type AB thymoma is not a mixed tumor of type A and type B thymomas, but a distinct type of thymoma. Virchows Arch. 2014;464:725-34 pubmed publisher
	Diaz E, Briggs M, Wen Y, Zhuang G, Wallace S, Dobberfuhl A, et al. Characterizing relaxin receptor expression and exploring relaxin's effect on tissue remodeling/fibrosis in the human bladder. BMC Urol. 2020;20:44 pubmed publisher
	Borgen E, Rypdal M, Sosa M, Renolen A, Schlichting E, Lønning P, et al. NR2F1 stratifies dormant disseminated tumor cells in breast cancer patients. Breast Cancer Res. 2018;20:120 pubmed publisher
	Saugier Veber P, Marguet F, Lecoquierre F, Adle Biassette H, Guimiot F, Cipriani S, et al. Hydrocephalus due to multiple ependymal malformations is caused by mutations in the MPDZ gene. Acta Neuropathol Commun. 2017;5:36 pubmed publisher
	Chiang J, Tan R, Wang J, Chen J, Lee Y, Hsieh P, et al. S100P, a calcium-binding protein, is preferentially associated with the growth of polypoid tumors in colorectal cancer. Int J Mol Med. 2015;35:675-83 pubmed publisher
	Mitchell A, Bendavid Y. Medullary colon cancer presenting with total necrosis of all regional lymph node metastases: morphologic description of a presumed immune-mediated event. Diagn Pathol. 2014;9:204 pubmed publisher
	Korytnaya E, Liu J, Camelo Piragua S, Sullivan S, Auchus R, Barkan A. Ectopic prolactin secretion from a perivascular epithelioid cell tumor (PEComa). J Clin Endocrinol Metab. 2014;99:3960-4 pubmed publisher
	Trietsch M, Spaans V, Ter Haar N, Osse E, Peters A, Gaarenstroom K, et al. CDKN2A(p16) and HRAS are frequently mutated in vulvar squamous cell carcinoma. Gynecol Oncol. 2014;135:149-55 pubmed publisher
	Hadidi A, Roessler J, Coerdt W. Development of the human male urethra: a histochemical study on human embryos. J Pediatr Surg. 2014;49:1146-52 pubmed publisher
	Caballero J, Gervasi M, Veiga M, Dalvit G, Perez Martinez S, Cetica P, et al. Epithelial cadherin is present in bovine oviduct epithelial cells and gametes, and is involved in fertilization-related events. Theriogenology. 2014;81:1189-206 pubmed publisher
	Karamitopoulou E, Zlobec I, Gloor B, Kondi Pafiti A, Lugli A, Perren A. Loss of Raf-1 kinase inhibitor protein (RKIP) is strongly associated with high-grade tumor budding and correlates with an aggressive phenotype in pancreatic ductal adenocarcinoma (PDAC). J Transl Med. 2013;11:311 pubmed publisher
	Jeong W, Lim W, Ahn S, Lim C, Lee J, Bae S, et al. Recrudescence mechanisms and gene expression profile of the reproductive tracts from chickens during the molting period. PLoS ONE. 2013;8:e76784 pubmed publisher
	Salvatore S, Reddi A, Chandran C, Chevalier J, Okechukwu C, Seshan S. Collapsing glomerulopathy superimposed on diabetic nephropathy: insights into etiology of an under-recognized, severe pattern of glomerular injury. Nephrol Dial Transplant. 2014;29:392-9 pubmed publisher
	Trietsch M, Peters A, Gaarenstroom K, van Koningsbrugge S, Ter Haar N, Osse E, et al. Spindle cell morphology is related to poor prognosis in vulvar squamous cell carcinoma. Br J Cancer. 2013;109:2259-65 pubmed publisher
	Guo Y, Chirieac L, Bueno R, Pass H, Wu W, Malinowska I, et al. Tsc1-Tp53 loss induces mesothelioma in mice, and evidence for this mechanism in human mesothelioma. Oncogene. 2014;33:3151-60 pubmed publisher
	Singh B, Bogatcheva G, Washington M, Coffey R. Transformation of polarized epithelial cells by apical mistrafficking of epiregulin. Proc Natl Acad Sci U S A. 2013;110:8960-5 pubmed publisher
	Heath C, Deep N, Beck L, Day K, Sweeny L, Zinn K, et al. Use of panitumumab-IRDye800 to image cutaneous head and neck cancer in mice. Otolaryngol Head Neck Surg. 2013;148:982-90 pubmed publisher
	Dayal J, Sales M, Corver W, Purdie C, Jordan L, Quinlan P, et al. Multiparameter DNA content analysis identifies distinct groups in primary breast cancer. Br J Cancer. 2013;108:873-80 pubmed publisher
	Wen Y, Wani P, Zhou L, Baer T, Phadnis S, Reijo Pera R, et al. Reprogramming of fibroblasts from older women with pelvic floor disorders alters cellular behavior associated with donor age. Stem Cells Transl Med. 2013;2:118-28 pubmed publisher
	Divito M, Rudisill S, Stein A, Patel S, McLaren J, Hubel A. Silica hybrid for corneal replacement: optical, biomechanical, and ex vivo biocompatibility studies. Invest Ophthalmol Vis Sci. 2012;53:8192-9 pubmed publisher
	Heath C, Deep N, Sweeny L, Zinn K, Rosenthal E. Use of panitumumab-IRDye800 to image microscopic head and neck cancer in an orthotopic surgical model. Ann Surg Oncol. 2012;19:3879-87 pubmed publisher
	Lu W, Schneider M, Neumann S, Jaeger V, Taranum S, Munck M, et al. Nesprin interchain associations control nuclear size. Cell Mol Life Sci. 2012;69:3493-509 pubmed publisher
	Barak S, Wang Z, Miettinen M. Immunoreactivity for calretinin and keratins in desmoid fibromatosis and other myofibroblastic tumors: a diagnostic pitfall. Am J Surg Pathol. 2012;36:1404-9 pubmed publisher
	Grilliot M, Goldblum J, Liu X. Signet-ring cell melanoma of the gastroesophageal junction: a case report and literature review. Arch Pathol Lab Med. 2012;136:324-8 pubmed publisher
	Balz L, Bartkowiak K, Andreas A, Pantel K, Niggemann B, Zanker K, et al. The interplay of HER2/HER3/PI3K and EGFR/HER2/PLC-?1 signalling in breast cancer cell migration and dissemination. J Pathol. 2012;227:234-44 pubmed publisher
	MARRERO B, Heller R. The use of an in vitro 3D melanoma model to predict in vivo plasmid transfection using electroporation. Biomaterials. 2012;33:3036-46 pubmed publisher
	Corver W, Ter Haar N, Fleuren G, Oosting J. Cervical carcinoma-associated fibroblasts are DNA diploid and do not show evidence for somatic genetic alterations. Cell Oncol (Dordr). 2011;34:553-63 pubmed publisher
	Grabinski N, Bartkowiak K, Grupp K, Brandt B, Pantel K, Jücker M. Distinct functional roles of Akt isoforms for proliferation, survival, migration and EGF-mediated signalling in lung cancer derived disseminated tumor cells. Cell Signal. 2011;23:1952-60 pubmed publisher
	Si Q, Dancer J, Stanton M, Tamboli P, Ro J, Czerniak B, et al. Small cell carcinoma of the kidney: a clinicopathologic study of 14 cases. Hum Pathol. 2011;42:1792-8 pubmed publisher
	Yan J, Diaz J, Jiao J, Wang R, You J. Perturbation of BRD4 protein function by BRD4-NUT protein abrogates cellular differentiation in NUT midline carcinoma. J Biol Chem. 2011;286:27663-75 pubmed publisher
	Geiersbach K, Rector L, Sederberg M, Hooker A, Randall R, Schiffman J, et al. Unknown partner for USP6 and unusual SS18 rearrangement detected by fluorescence in situ hybridization in a solid aneurysmal bone cyst. Cancer Genet. 2011;204:195-202 pubmed publisher
	McLachlan G, Davidson H, Holder E, Davies L, Pringle I, Sumner Jones S, et al. Pre-clinical evaluation of three non-viral gene transfer agents for cystic fibrosis after aerosol delivery to the ovine lung. Gene Ther. 2011;18:996-1005 pubmed publisher
	Boncher J, Bronner M, Goldblum J, Liu X. Reticulin staining clarifies florid benign signet ring cell change with mitotic activity in a penetrating gastric ulcer. Am J Surg Pathol. 2011;35:762-6 pubmed publisher
	Sänger N, Effenberger K, Riethdorf S, Van Haasteren V, Gauwerky J, Wiegratz I, et al. Disseminated tumor cells in the bone marrow of patients with ductal carcinoma in situ. Int J Cancer. 2011;129:2522-6 pubmed publisher
	McArdle A, Maduwegedera D, Moritz K, Flower R, Denton K, Roberts C. Chronic maternal hypertension affects placental gene expression and differentiation in rabbits. J Hypertens. 2010;28:959-68 pubmed
	Morimoto Tochigi A, Walkup R, Nakajima E, Shearer T, Azuma M. Mechanism for carbachol-induced secretion of lacritin in cultured monkey lacrimal acinar cells. Invest Ophthalmol Vis Sci. 2010;51:4395-406 pubmed publisher
	Apple S, Moatamed N, Finck R, Sullivan P. Accurate classification of sentinel lymph node metastases in patients with lobular breast carcinoma. Breast. 2010;19:360-4 pubmed publisher
	Furusato E, Cameron J, Newsom R, Fujishiro T, Kojima T, Specht C, et al. Ocular perivascular epithelioid cell tumor: report of 2 cases with distinct clinical presentations. Hum Pathol. 2010;41:768-72 pubmed publisher
	Carpentino J, Hynes M, Appelman H, Zheng T, Steindler D, Scott E, et al. Aldehyde dehydrogenase-expressing colon stem cells contribute to tumorigenesis in the transition from colitis to cancer. Cancer Res. 2009;69:8208-15 pubmed publisher
	Sohsman M, Yang H, Cassarino D. Sinonasal undifferentiated carcinoma metastatic to the skin. J Cutan Pathol. 2010;37:1241-4 pubmed publisher
	MARRERO B, Messina J, Heller R. Generation of a tumor spheroid in a microgravity environment as a 3D model of melanoma. In Vitro Cell Dev Biol Anim. 2009;45:523-34 pubmed publisher
	Bidlingmaier S, He J, Wang Y, An F, Feng J, Barbone D, et al. Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 2009;69:1570-7 pubmed publisher
	Limaye P, Bowen W, Orr A, Luo J, Tseng G, Michalopoulos G. Mechanisms of hepatocyte growth factor-mediated and epidermal growth factor-mediated signaling in transdifferentiation of rat hepatocytes to biliary epithelium. Hepatology. 2008;47:1702-13 pubmed publisher
	Resto V, Burdick M, Dagia N, McCammon S, Fennewald S, Sackstein R. L-selectin-mediated lymphocyte-cancer cell interactions under low fluid shear conditions. J Biol Chem. 2008;283:15816-24 pubmed publisher
	Maire G, Brown C, Bayani J, Pereira C, Gravel D, Bell J, et al. Complex rearrangement of chromosomes 19, 21, and 22 in Ewing sarcoma involving a novel reciprocal inversion-insertion mechanism of EWS-ERG fusion gene formation: a case analysis and literature review. Cancer Genet Cytogenet. 2008;181:81-92 pubmed publisher
	Heinzman J, Brower S, Bush J, Silverman J. Ex vivo enrichment of malignant carcinoma cells in primary culture. Pathology. 2007;39:491-4 pubmed
	Kusano K, Tokunaga O, Ando T, Inokuchi A. Helicobacter pylori in the palatine tonsils of patients with IgA nephropathy compared with those of patients with recurrent pharyngotonsillitis. Hum Pathol. 2007;38:1788-97 pubmed
	Scoles D, Pavelka J, Cass I, Tran H, Baldwin R, Armstrong K, et al. Characterization of CSOC 882, a novel immortalized ovarian cancer cell line expressing EGFR, HER2, and activated AKT. Gynecol Oncol. 2007;104:120-8 pubmed
	Matthai C, Horvat R, Noe M, Nagele F, Radjabi A, van Trotsenburg M, et al. Oct-4 expression in human endometrium. Mol Hum Reprod. 2006;12:7-10 pubmed
	Aydin H, Young R, Ronnett B, Epstein J. Clear cell papillary cystadenoma of the epididymis and mesosalpinx: immunohistochemical differentiation from metastatic clear cell renal cell carcinoma. Am J Surg Pathol. 2005;29:520-3 pubmed

product information

Catalog Number :

MAB3412

Subcategory :

Cell Structure

Product Name :

Anti-Cytokeratin AE1/AE3 Antibody, recognizes acidic & basic cytokeratins, clone AE1/AE3

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

Cytokeratin AE1/AE3

Clone :

AE1/AE3

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Cytokeratin AE1/AE3 Antibody, recognizes acidic & basic cytokeratins, clone AE1/AE3

Cross Reactivity :

Human;Rat;Mouse;Monkey;Rabbit;Bovine;Chicken

Background :

Keratins are a group of water-insoluble proteins that form monofilaments, a class of intermediate filament. These filaments form part of the cytoskeletal complex in epidermis and in most other epithelial tissues. Nineteen human epithelial keratins are resolved with two-dimensional gels electrophoresis (Moll et al., 1982). These can be divided into acid (pI 5.7) and basic (pI 6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kDa. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56, and 52 kDa.Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state (Sun et al., 1984; Cooper et al., 1985; Sun et al., 1985);The 56.5/65-67 kDa pair is present in keratinized (differentiated) epidermis;The 55/64 kDa pair is characteristic of normal (corneal-type) epithelial differentiation (Moll et al., 1982; Sun et al., 1984);The 51/59 kDa pair is characteristic of the stratified squamous epithelial of internal organism such as esophagus and tongue (Moll et al., 1982; Cooper et al., 1985);The 51/58 kDa pair is a keratinocyte marker; the pair is present in almost all stratified epithelia irrespective of the state of cellular stratification (Moll et al., 1982; Sun et al., 1984);The 48/56 kD pair is characteristic of hyperproliferative (de-differentiated) keratinocytes (Moll et al., 1982; Weiss et al., 1984)t;The 45/52 kD pair and to a lesser extent, the 46/54 kDa pair are characteristic of simple epithelia (Moll et al., 1982);The 40 kD keratin is present in most epithelia except adult epidermis (Moll et al., 1982).

ALT Names :

Pan cytokeratin

Immunogen :

Human epidermal keratins

Specificity :

The stringent, but broad, specificity of pooled AE1/AE3 antibody has made this preparation very useful as a general stain for normal and neoplastic cells of epithelial origin. Anti-Keratin AE1 recognizes the 56.5, 50, 50’, 48, and 40 kD keratins of the acidic subfamily. Anti-keratin AE3 recognizes all members of the basic subfamily {65,67,64,59.59,56,52 human basic cytokeratins}.

Package Size :

500 µg

Uses :

ELISA;Immunohistochemistry;Western Blotting

Storage :

Stable for 1 year at 2-8ºC from date of receipt. DO NOT FREEZE.