This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Tubulin Antibody, beta, clone KMX-1
catalog :
MAB3408
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
KMX-1
reactivity :
human, mouse, chicken, fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 80
Published Application/Species/Sample/DilutionReference
  • western blot; chicken; fig 2
Akizu N, García M, Estarás C, Fueyo R, Badosa C, de la Cruz X, et al. EZH2 regulates neuroepithelium structure and neuroblast proliferation by repressing p21. Open Biol. 2016;6:150227 pubmed publisher
  • western blot; human; fig 7
Köhler C, Koalick D, Fabricius A, Parplys A, Borgmann K, Pospiech H, et al. Cdc45 is limiting for replication initiation in humans. Cell Cycle. 2016;15:974-85 pubmed publisher
  • western blot; human
Sokka M, Rilla K, Miinalainen I, Pospiech H, Syväoja J. High levels of TopBP1 induce ATR-dependent shut-down of rRNA transcription and nucleolar segregation. Nucleic Acids Res. 2015;43:4975-89 pubmed publisher
  • western blot; mouse; 1:1000; fig 1
Qiao S, Dennis M, Song X, Vadysirisack D, Salunke D, Nash Z, et al. A REDD1/TXNIP pro-oxidant complex regulates ATG4B activity to control stress-induced autophagy and sustain exercise capacity. Nat Commun. 2015;6:7014 pubmed publisher
  • western blot; mouse; fig 3
Völkel S, Stielow B, Finkernagel F, Stiewe T, Nist A, Suske G. Zinc finger independent genome-wide binding of Sp2 potentiates recruitment of histone-fold protein Nf-y distinguishing it from Sp1 and Sp3. PLoS Genet. 2015;11:e1005102 pubmed publisher
  • western blot; human; 1:2000
Muenz T, Groh C, Maisonnasse A, Le Conte Y, Plettner E, Rössler W. Neuronal plasticity in the mushroom body calyx during adult maturation in the honeybee and possible pheromonal influences. Dev Neurobiol. 2015;75:1368-84 pubmed publisher
  • western blot; human
Khalouei S, Chow A, Brown I. Localization of heat shock protein HSPA6 (HSP70B') to sites of transcription in cultured differentiated human neuronal cells following thermal stress. J Neurochem. 2014;131:743-54 pubmed publisher
  • immunohistochemistry; human
Kempf A, Tews B, Arzt M, Weinmann O, Obermair F, Pernet V, et al. The sphingolipid receptor S1PR2 is a receptor for Nogo-a repressing synaptic plasticity. PLoS Biol. 2014;12:e1001763 pubmed publisher
  • immunocytochemistry; human
Slim C, Lázaro Diéguez F, Bijlard M, Toussaint M, de Bruin A, Du Q, et al. Par1b induces asymmetric inheritance of plasma membrane domains via LGN-dependent mitotic spindle orientation in proliferating hepatocytes. PLoS Biol. 2013;11:e1001739 pubmed publisher
  • western blot; human
Khalouei S, Chow A, Brown I. Stress-induced localization of HSPA6 (HSP70B') and HSPA1A (HSP70-1) proteins to centrioles in human neuronal cells. Cell Stress Chaperones. 2014;19:321-7 pubmed publisher
  • western blot; mouse
Eguchi J, Kong X, Tenta M, Wang X, Kang S, Rosen E. Interferon regulatory factor 4 regulates obesity-induced inflammation through regulation of adipose tissue macrophage polarization. Diabetes. 2013;62:3394-403 pubmed publisher
  • western blot; fruit fly
Meier K, Mathieu E, Finkernagel F, Reuter L, Scharfe M, Doehlemann G, et al. LINT, a novel dL(3)mbt-containing complex, represses malignant brain tumour signature genes. PLoS Genet. 2012;8:e1002676 pubmed publisher
Agnew E, Garcia Burgos A, Richardson R, Manos H, Thomson A, Sooy K, et al. Antenatal dexamethasone treatment transiently alters diastolic function in the mouse fetal heart. J Endocrinol. 2019;241:279-292 pubmed publisher
Richter M, Ludewig S, Winschel A, Abel T, Bold C, Salzburger L, et al. Distinct in vivo roles of secreted APP ectodomain variants APPsα and APPsβ in regulation of spine density, synaptic plasticity, and cognition. EMBO J. 2018;37: pubmed publisher
Bolin S, Borgenvik A, Persson C, Sundström A, Qi J, Bradner J, et al. Combined BET bromodomain and CDK2 inhibition in MYC-driven medulloblastoma. Oncogene. 2018;37:2850-2862 pubmed publisher
Fueyo R, Iacobucci S, PAPPA S, Estarás C, Lois S, Vicioso Mantis M, et al. Lineage specific transcription factors and epigenetic regulators mediate TGF?-dependent enhancer activation. Nucleic Acids Res. 2018;46:3351-3365 pubmed publisher
Stielow B, Finkernagel F, Stiewe T, Nist A, Suske G. MGA, L3MBTL2 and E2F6 determine genomic binding of the non-canonical Polycomb repressive complex PRC1.6. PLoS Genet. 2018;14:e1007193 pubmed publisher
Zhang Z, Coutinho A, Man T, Kipari T, Hadoke P, Salter D, et al. Macrophage 11β-HSD-1 deficiency promotes inflammatory angiogenesis. J Endocrinol. 2017;234:291-299 pubmed publisher
Donnio L, Bidon B, Hashimoto S, May M, Epanchintsev A, Ryan C, et al. MED12-related XLID disorders are dose-dependent of immediate early genes (IEGs) expression. Hum Mol Genet. 2017;26:2062-2075 pubmed publisher
Asensio Juan E, Fueyo R, PAPPA S, Iacobucci S, Badosa C, Lois S, et al. The histone demethylase PHF8 is a molecular safeguard of the IFNγ response. Nucleic Acids Res. 2017;45:3800-3811 pubmed publisher
Novak S, Joardar A, Gregorio C, Zarnescu D. Regulation of Heart Rate in Drosophila via Fragile X Mental Retardation Protein. PLoS ONE. 2015;10:e0142836 pubmed publisher
Sooy K, Noble J, McBride A, Binnie M, Yau J, Seckl J, et al. Cognitive and Disease-Modifying Effects of 11β-Hydroxysteroid Dehydrogenase Type 1 Inhibition in Male Tg2576 Mice, a Model of Alzheimer's Disease. Endocrinology. 2015;156:4592-603 pubmed publisher
Hayashi S, Okada Y. Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics. Mol Biol Cell. 2015;26:1743-51 pubmed publisher
Coyne A, Siddegowda B, Estes P, Johannesmeyer J, Kovalik T, Daniel S, et al. Futsch/MAP1B mRNA is a translational target of TDP-43 and is neuroprotective in a Drosophila model of amyotrophic lateral sclerosis. J Neurosci. 2014;34:15962-74 pubmed publisher
Traboulsi H, Davoli S, Catez P, Egly J, Compe E. Dynamic partnership between TFIIH, PGC-1α and SIRT1 is impaired in trichothiodystrophy. PLoS Genet. 2014;10:e1004732 pubmed publisher
Docanto M, Yang F, Callaghan B, Au C, Ragavan R, Wang X, et al. Ghrelin and des-acyl ghrelin inhibit aromatase expression and activity in human adipose stromal cells: suppression of cAMP as a possible mechanism. Breast Cancer Res Treat. 2014;147:193-201 pubmed publisher
Frederick D, Salas Fragomeni R, Schalck A, Ferreiro Neira I, Hoff T, Cooper Z, et al. Clinical profiling of BCL-2 family members in the setting of BRAF inhibition offers a rationale for targeting de novo resistance using BH3 mimetics. PLoS ONE. 2014;9:e101286 pubmed publisher
Rhie S, Hazelett D, Coetzee S, Yan C, Noushmehr H, Coetzee G. Nucleosome positioning and histone modifications define relationships between regulatory elements and nearby gene expression in breast epithelial cells. BMC Genomics. 2014;15:331 pubmed publisher
Tojima T, Itofusa R, Kamiguchi H. Steering neuronal growth cones by shifting the imbalance between exocytosis and endocytosis. J Neurosci. 2014;34:7165-78 pubmed publisher
Czech D, Lee J, Correia J, Loke H, Möller E, Harley V. Transient neuroprotection by SRY upregulation in dopamine cells following injury in males. Endocrinology. 2014;155:2602-12 pubmed publisher
Chow A, Tang D, Hanif A, Brown I. Localization of heat shock proteins in cerebral cortical cultures following induction by celastrol. Cell Stress Chaperones. 2014;19:845-51 pubmed publisher
Weyer S, Zagrebelsky M, Herrmann U, Hick M, Ganss L, Gobbert J, et al. Comparative analysis of single and combined APP/APLP knockouts reveals reduced spine density in APP-KO mice that is prevented by APPs? expression. Acta Neuropathol Commun. 2014;2:36 pubmed publisher
Minter M, Zhang M, Ates R, Taylor J, Crack P. Type-1 interferons contribute to oxygen glucose deprivation induced neuro-inflammation in BE(2)M17 human neuroblastoma cells. J Neuroinflammation. 2014;11:43 pubmed publisher
Taylor J, Minter M, Newman A, Zhang M, Adlard P, Crack P. Type-1 interferon signaling mediates neuro-inflammatory events in models of Alzheimer's disease. Neurobiol Aging. 2014;35:1012-23 pubmed publisher
Lazarus K, Zhao Z, Knower K, To S, Chand A, Clyne C. Oestradiol reduces liver receptor homolog-1 mRNA transcript stability in breast cancer cell lines. Biochem Biophys Res Commun. 2013;438:533-9 pubmed publisher
BRUSES J. Cell surface localization of ?3?4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility. PLoS ONE. 2013;8:e62435 pubmed publisher
La Torre A, Del Mar Masdeu M, Cotrufo T, Moubarak R, Del Rio J, Comella J, et al. A role for the tyrosine kinase ACK1 in neurotrophin signaling and neuronal extension and branching. Cell Death Dis. 2013;4:e602 pubmed publisher
Márquez Navarro A, Pérez Reyes A, Zepeda Rodriguez A, Reynoso Ducoing O, Hernández Campos A, Hernández Luis F, et al. RCB20, an experimental benzimidazole derivative, affects tubulin expression and induces gross anatomical changes in Taenia crassiceps cysticerci. Parasitol Res. 2013;112:2215-26 pubmed publisher
Estarás C, Fueyo R, Akizu N, Beltran S, Martínez Balbás M. RNA polymerase II progression through H3K27me3-enriched gene bodies requires JMJD3 histone demethylase. Mol Biol Cell. 2013;24:351-60 pubmed publisher
Flannery R, BRUSES J. N-cadherin induces partial differentiation of cholinergic presynaptic terminals in heterologous cultures of brainstem neurons and CHO cells. Front Synaptic Neurosci. 2012;4:6 pubmed publisher
Giambra V, Jenkins C, Wang H, Lam S, Shevchuk O, Nemirovsky O, et al. NOTCH1 promotes T cell leukemia-initiating activity by RUNX-mediated regulation of PKC-? and reactive oxygen species. Nat Med. 2012;18:1693-8 pubmed publisher
Stein C, Riedl S, Rüthnick D, Nötzold R, Bauer U. The arginine methyltransferase PRMT6 regulates cell proliferation and senescence through transcriptional repression of tumor suppressor genes. Nucleic Acids Res. 2012;40:9522-33 pubmed publisher
Lloret Llinares M, Pérez Lluch S, Rossell D, Morán T, Ponsa Cobas J, Auer H, et al. dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes. Nucleic Acids Res. 2012;40:9493-505 pubmed publisher
Henson M, Larsen R, Lawson S, Pérez Otaño I, Nakanishi N, Lipton S, et al. Genetic deletion of NR3A accelerates glutamatergic synapse maturation. PLoS ONE. 2012;7:e42327 pubmed publisher
Asensio Juan E, Gallego C, Martínez Balbás M. The histone demethylase PHF8 is essential for cytoskeleton dynamics. Nucleic Acids Res. 2012;40:9429-40 pubmed publisher
Terrados G, Finkernagel F, Stielow B, Sadic D, Neubert J, Herdt O, et al. Genome-wide localization and expression profiling establish Sp2 as a sequence-specific transcription factor regulating vitally important genes. Nucleic Acids Res. 2012;40:7844-57 pubmed publisher
Czech D, Lee J, Sim H, Parish C, Vilain E, Harley V. The human testis-determining factor SRY localizes in midbrain dopamine neurons and regulates multiple components of catecholamine synthesis and metabolism. J Neurochem. 2012;122:260-71 pubmed publisher
Wong H, Shimizu A, Kirkpatrick N, Garkavtsev I, Chan A, di Tomaso E, et al. Merlin/NF2 regulates angiogenesis in schwannomas through a Rac1/semaphorin 3F-dependent mechanism. Neoplasia. 2012;14:84-94 pubmed
Zhang L, Widau R, Herring B, Gallagher P. Delta-like 1-Lysine613 regulates notch signaling. Biochim Biophys Acta. 2011;1813:2036-43 pubmed publisher
Mollerup S, Hofgaard J, Braunstein T, Kjenseth A, Leithe E, Rivedal E, et al. Norepinephrine inhibits intercellular coupling in rat cardiomyocytes by ubiquitination of connexin43 gap junctions. Cell Commun Adhes. 2011;18:57-65 pubmed publisher
Schneider A, Mehmood T, Pannetier S, Hanauer A. Altered ERK/MAPK signaling in the hippocampus of the mrsk2_KO mouse model of Coffin-Lowry syndrome. J Neurochem. 2011;119:447-59 pubmed publisher
Gould T, Myers J, Bewersdorf J. Total internal reflection STED microscopy. Opt Express. 2011;19:13351-7 pubmed publisher
Leung K, Riley F, Carrington M, Field M. Ubiquitylation and developmental regulation of invariant surface protein expression in trypanosomes. Eukaryot Cell. 2011;10:916-31 pubmed publisher
Sareh H, Tulapurkar M, Shah N, Singh I, Hasday J. Response of mice to continuous 5-day passive hyperthermia resembles human heat acclimation. Cell Stress Chaperones. 2011;16:297-307 pubmed publisher
Harrington E, Zhao C, Fancy S, Kaing S, Franklin R, Rowitch D. Oligodendrocyte PTEN is required for myelin and axonal integrity, not remyelination. Ann Neurol. 2010;68:703-16 pubmed publisher
Nelson A, Holt J. Impact of RING and BRCT domain mutations on BRCA1 protein stability, localization and recruitment to DNA damage. Radiat Res. 2010;174:1-13 pubmed publisher
Chen L, Wang Y, Tseng G. Compression alters kinase and phosphatase activity and tau and MAP2 phosphorylation transiently while inducing the fast adaptive dendritic remodeling of underlying cortical neurons. J Neurotrauma. 2010;27:1657-69 pubmed publisher
Hensley K, Christov A, Kamat S, Zhang X, Jackson K, Snow S, et al. Proteomic identification of binding partners for the brain metabolite lanthionine ketimine (LK) and documentation of LK effects on microglia and motoneuron cell cultures. J Neurosci. 2010;30:2979-88 pubmed publisher
Chow A, Mok P, Xiao D, Khalouei S, Brown I. Heteromeric complexes of heat shock protein 70 (HSP70) family members, including Hsp70B', in differentiated human neuronal cells. Cell Stress Chaperones. 2010;15:545-53 pubmed publisher
Liao S, Wang T, Fan K, Tu X. The small ubiquitin-like modifier (SUMO) is essential in cell cycle regulation in Trypanosoma brucei. Exp Cell Res. 2010;316:704-15 pubmed publisher
Procida K, Jørgensen L, Schmitt N, Delmar M, Taffet S, Holstein Rathlou N, et al. Phosphorylation of connexin43 on serine 306 regulates electrical coupling. Heart Rhythm. 2009;6:1632-8 pubmed publisher
Maouche K, Polette M, Jolly T, Medjber K, Cloëz Tayarani I, Changeux J, et al. {alpha}7 nicotinic acetylcholine receptor regulates airway epithelium differentiation by controlling basal cell proliferation. Am J Pathol. 2009;175:1868-82 pubmed publisher
Silveira E Souza A, Trindade E, Jamur M, Oliver C. Gangliosides are important for the preservation of the structure and organization of RBL-2H3 mast cells. J Histochem Cytochem. 2010;58:83-93 pubmed publisher
Ghose S, Gleason K, Potts B, Lewis Amezcua K, Tamminga C. Differential expression of metabotropic glutamate receptors 2 and 3 in schizophrenia: a mechanism for antipsychotic drug action?. Am J Psychiatry. 2009;166:812-20 pubmed publisher
Bakshi K, Gennaro S, CHAN C, Kosciuk M, Liu J, Stucky A, et al. Prenatal cocaine reduces AMPA receptor synaptic expression through hyperphosphorylation of the synaptic anchoring protein GRIP. J Neurosci. 2009;29:6308-19 pubmed publisher
Mannefeld M, Klassen E, Gaubatz S. B-MYB is required for recovery from the DNA damage-induced G2 checkpoint in p53 mutant cells. Cancer Res. 2009;69:4073-80 pubmed publisher
Mace E, Monkley S, Critchley D, Takei F. A dual role for talin in NK cell cytotoxicity: activation of LFA-1-mediated cell adhesion and polarization of NK cells. J Immunol. 2009;182:948-56 pubmed
Kikuchi K, Takahashi K. WAVE2- and microtubule-dependent formation of long protrusions and invasion of cancer cells cultured on three-dimensional extracellular matrices. Cancer Sci. 2008;99:2252-9 pubmed publisher
Parikh N, Nagarajan P, Sei ichi M, Sinha S, Garrett Sinha L. Isolation and characterization of an immortalized oral keratinocyte cell line of mouse origin. Arch Oral Biol. 2008;53:1091-100 pubmed publisher
Kleinschmidt M, Streubel G, Samans B, Krause M, Bauer U. The protein arginine methyltransferases CARM1 and PRMT1 cooperate in gene regulation. Nucleic Acids Res. 2008;36:3202-13 pubmed publisher
Xu D, Li H, Liu J. Inhibition of telomerase by targeting MAP kinase signaling. Methods Mol Biol. 2007;405:147-65 pubmed publisher
Chow A, Brown I. Induction of heat shock proteins in differentiated human and rodent neurons by celastrol. Cell Stress Chaperones. 2007;12:237-44 pubmed
Medvedik O, Lamming D, Kim K, Sinclair D. MSN2 and MSN4 link calorie restriction and TOR to sirtuin-mediated lifespan extension in Saccharomyces cerevisiae. PLoS Biol. 2007;5:e261 pubmed
Schmit F, Korenjak M, Mannefeld M, Schmitt K, Franke C, von Eyss B, et al. LINC, a human complex that is related to pRB-containing complexes in invertebrates regulates the expression of G2/M genes. Cell Cycle. 2007;6:1903-13 pubmed
Ring S, Weyer S, Kilian S, Waldron E, Pietrzik C, Filippov M, et al. The secreted beta-amyloid precursor protein ectodomain APPs alpha is sufficient to rescue the anatomical, behavioral, and electrophysiological abnormalities of APP-deficient mice. J Neurosci. 2007;27:7817-26 pubmed
Paccagnini E, Mencarelli C, Mercati D, Afzelius B, Dallai R. Ultrastructural analysis of the aberrant axoneme morphogenesis in thrips (Thysanoptera, Insecta). Cell Motil Cytoskeleton. 2007;64:645-61 pubmed
Nowak K, Killmer K, Gessner C, Lutz W. E2F-1 regulates expression of FOXO1 and FOXO3a. Biochim Biophys Acta. 2007;1769:244-52 pubmed
Spofford L, Abel E, Boisvert Adamo K, Aplin A. Cyclin D3 expression in melanoma cells is regulated by adhesion-dependent phosphatidylinositol 3-kinase signaling and contributes to G1-S progression. J Biol Chem. 2006;281:25644-51 pubmed
Nowak K, Kerl K, Fehr D, Kramps C, Gessner C, Killmer K, et al. BMI1 is a target gene of E2F-1 and is strongly expressed in primary neuroblastomas. Nucleic Acids Res. 2006;34:1745-54 pubmed
Wang D, John S, Clements J, Percy D, Barton K, Garrett Sinha L. Ets-1 deficiency leads to altered B cell differentiation, hyperresponsiveness to TLR9 and autoimmune disease. Int Immunol. 2005;17:1179-91 pubmed
product information
Catalog Number :
MAB3408
Subcategory :
Cell Structure
Product Name :
Anti-Tubulin Antibody, beta, clone KMX-1
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
Q13509
Host Name :
Mouse
Antigen :
Tubulin
Clone :
KMX-1
Conjugate :
Purified
Isotype :
IgG2b
Product Description :
Anti-Tubulin Antibody, beta, clone KMX-1
Cross Reactivity :
All;Human;Mouse;Rat
Background :
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.
ALT Names :
Tubulin beta-4;Tubulin beta-III;tubulin, beta 3;tubulin, beta, 4
Immunogen :
Tubulin purified from polycephalum myxamoebae
Specificity :
This antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only.
Package Size :
50 µg
Uses :
Immunocytochemistry;Western Blotting
Storage :
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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