This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Vimentin Antibody, clone V9
catalog :
MAB3400
quantity :
40 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
V9

The same clone is also sold as:
reactivity :
jirds, African green monkey, black ferret, human, mouse, rat, dogs, pigs , zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 62
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; loading ...; fig 5c
Smillie C, Biton M, Ordovas Montanes J, Sullivan K, Burgin G, Graham D, et al. Intra- and Inter-cellular Rewiring of the Human Colon during Ulcerative Colitis. Cell. 2019;178:714-730.e22 pubmed publisher
  • immunocytochemistry; black ferret; 1:400; loading ...; fig 4d
Mart nez Mart nez M, Ciceri G, Espin s A, Fern ndez V, Mar n O, Borrell V. Extensive branching of radially-migrating neurons in the mammalian cerebral cortex. J Comp Neurol. 2019;527:1558-1576 pubmed publisher
  • immunohistochemistry; jirds; 1:100; loading ...; tbl 1
Hammoum I, Benlarbi M, Dellaa A, Szabó K, Dékány B, Csaba D, et al. Study of retinal neurodegeneration and maculopathy in diabetic Meriones shawi: A particular animal model with human-like macula. J Comp Neurol. 2017;525:2890-2914 pubmed publisher
  • immunohistochemistry; human; 1:100; loading ...; fig 5c
Kuo W, Hwu L, Wu C, Lee J, Chang C, Liu R. STAT3/NF-?B-Regulated Lentiviral TK/GCV Suicide Gene Therapy for Cisplatin-Resistant Triple-Negative Breast Cancer. Theranostics. 2017;7:647-663 pubmed publisher
  • immunocytochemistry; rat; loading ...; fig s6a
López Montero N, Ramos Marquès E, Risco C, Garcia del Portillo F. Intracellular Salmonella induces aggrephagy of host endomembranes in persistent infections. Autophagy. 2016;12:1886-1901 pubmed
  • western blot; mouse; 1:1000; fig 1c
Cozzolino A, Noce V, Battistelli C, Marchetti A, Grassi G, Cicchini C, et al. Modulating the Substrate Stiffness to Manipulate Differentiation of Resident Liver Stem Cells and to Improve the Differentiation State of Hepatocytes. Stem Cells Int. 2016;2016:5481493 pubmed publisher
  • immunohistochemistry; rat; fig s5
Carabalona A, Hu D, Vallee R. KIF1A inhibition immortalizes brain stem cells but blocks BDNF-mediated neuronal migration. Nat Neurosci. 2016;19:253-62 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:300; fig 6
Hui S, Nag T, Ghosh S. Characterization of Proliferating Neural Progenitors after Spinal Cord Injury in Adult Zebrafish. PLoS ONE. 2015;10:e0143595 pubmed publisher
  • immunohistochemistry; human; 1:1000
  • western blot; human; 1:100
ORELLANA R, Kato S, Erices R, Bravo M, Gonzalez P, Oliva B, et al. Platelets enhance tissue factor protein and metastasis initiating cell markers, and act as chemoattractants increasing the migration of ovarian cancer cells. BMC Cancer. 2015;15:290 pubmed publisher
  • immunocytochemistry; rat; 1:1000
Ettle B, Reiprich S, Deusser J, Schlachetzki J, Xiang W, Prots I, et al. Intracellular alpha-synuclein affects early maturation of primary oligodendrocyte progenitor cells. Mol Cell Neurosci. 2014;62:68-78 pubmed publisher
  • western blot; pigs ; 1:200
Uh K, Park C, Choi K, Park J, Jeong Y, Roh S, et al. Analysis of imprinted IGF2/H19 gene methylation and expression in normal fertilized and parthenogenetic embryonic stem cells of pigs. Anim Reprod Sci. 2014;147:47-55 pubmed publisher
  • immunocytochemistry; human; 1:100
Mato E, Gonzalez C, Moral A, Pérez J, Bell O, Lerma E, et al. ABCG2/BCRP gene expression is related to epithelial-mesenchymal transition inducer genes in a papillary thyroid carcinoma cell line (TPC-1). J Mol Endocrinol. 2014;52:289-300 pubmed publisher
  • immunocytochemistry; human; 1:50
Polling S, Mok Y, Ramdzan Y, Turner B, Yerbury J, Hill A, et al. Misfolded polyglutamine, polyalanine, and superoxide dismutase 1 aggregate via distinct pathways in the cell. J Biol Chem. 2014;289:6669-80 pubmed publisher
  • western blot; human
Oshiro N, Rapley J, Avruch J. Amino acids activate mammalian target of rapamycin (mTOR) complex 1 without changing Rag GTPase guanyl nucleotide charging. J Biol Chem. 2014;289:2658-74 pubmed publisher
  • immunocytochemistry; dogs
Tobias I, Brooks C, Teichroeb J, Betts D. Derivation and culture of canine embryonic stem cells. Methods Mol Biol. 2013;1074:69-83 pubmed publisher
  • immunocytochemistry; rat; 1:200; fig 1h
Valdés Sánchez T, Rodríguez Jiménez F, García Cruz D, Escobar Ivirico J, Alastrue Agudo A, Erceg S, et al. Methacrylate-endcapped caprolactone and FM19G11 provide a proper niche for spinal cord-derived neural cells. J Tissue Eng Regen Med. 2015;9:734-9 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:2000
Schwartz C, Cheng A, Mughal M, Mattson M, Yao P. Clathrin assembly proteins AP180 and CALM in the embryonic rat brain. J Comp Neurol. 2010;518:3803-18 pubmed publisher
  • immunohistochemistry - frozen section; African green monkey; 1:1000
Gil Perotin S, Duran Moreno M, Belzunegui S, Luquin M, Garcia Verdugo J. Ultrastructure of the subventricular zone in Macaca fascicularis and evidence of a mouse-like migratory stream. J Comp Neurol. 2009;514:533-54 pubmed publisher
  • immunohistochemistry; rat; 1:500
Talos D, Fishman R, Park H, Folkerth R, Follett P, Volpe J, et al. Developmental regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor subunit expression in forebrain and relationship to regional susceptibility to hypoxic/ischemic injury. I. Rodent cerebral white matter and cortex. J Comp Neurol. 2006;497:42-60 pubmed
  • immunocytochemistry; human
Wenisch S, Trinkaus K, Hild A, Hose D, Heiss C, Alt V, et al. Immunochemical, ultrastructural and electrophysiological investigations of bone-derived stem cells in the course of neuronal differentiation. Bone. 2006;38:911-21 pubmed
  • immunohistochemistry - frozen section; rat; 1:100
Mignon L, Vourc H P, Romero Ramos M, Osztermann P, Young H, Lucas P, et al. Transplantation of multipotent cells extracted from adult skeletal muscles into the subventricular zone of adult rats. J Comp Neurol. 2005;491:96-108 pubmed
Blair J, Hockemeyer D, Bateup H. Genetically engineered human cortical spheroid models of tuberous sclerosis. Nat Med. 2018;24:1568-1578 pubmed publisher
Lafont E, Kantari Mimoun C, Dráber P, De Miguel D, Hartwig T, Reichert M, et al. The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death. EMBO J. 2017;36:1147-1166 pubmed publisher
Ishikawa M, Yoshitomi T, Zorumski C, Izumi Y. Neurosteroids are endogenous neuroprotectants in an ex vivo glaucoma model. Invest Ophthalmol Vis Sci. 2014;55:8531-41 pubmed publisher
Qendro V, Lundgren D, Rezaul K, Mahony F, Ferrell N, Bi A, et al. Large-scale proteomic characterization of melanoma expressed proteins reveals nestin and vimentin as biomarkers that can potentially distinguish melanoma subtypes. J Proteome Res. 2014;13:5031-40 pubmed publisher
Cheema M, Damkier H, Nielsen J, Poulsen E, Enghild J, Fenton R, et al. Distal renal tubules are deficient in aggresome formation and autophagy upon aldosterone administration. PLoS ONE. 2014;9:e101258 pubmed publisher
Kojima S, Borisy G. An image-based, dual fluorescence reporter assay to evaluate the efficacy of shRNA for gene silencing at the single-cell level. F1000Res. 2014;3:60 pubmed publisher
Wittmann G, Harney J, Singru P, Nouriel S, Reed Larsen P, Lechan R. Inflammation-inducible type 2 deiodinase expression in the leptomeninges, choroid plexus, and at brain blood vessels in male rodents. Endocrinology. 2014;155:2009-19 pubmed publisher
Cheema M, Poulsen E, Enghild J, Hoorn E, Hoorn E, Fenton R, et al. Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules. Physiol Rep. 2013;1:e00064 pubmed publisher
Lazzari M, Bettini S, Franceschini V. Immunocytochemical characterisation of olfactory ensheathing cells of zebrafish. J Anat. 2014;224:192-206 pubmed publisher
Han X, Zhao L, Lu G, Ge J, Zhao Y, Zu S, et al. Improving outcomes of acute kidney injury using mouse renal progenitor cells alone or in combination with erythropoietin or suramin. Stem Cell Res Ther. 2013;4:74 pubmed publisher
Jeong H, Ji K, Kim J, Jou I, Joe E. Repair of astrocytes, blood vessels, and myelin in the injured brain: possible roles of blood monocytes. Mol Brain. 2013;6:28 pubmed publisher
Yamaguchi H, Hasegawa K, Esumi M. Protein from the fraction remaining after RNA extraction is useful for proteomics but care must be exercised in its application. Exp Mol Pathol. 2013;95:46-50 pubmed publisher
Lin S, Lee Y, Wang J, Miller S, Chiou S, Hung M, et al. Survival of cancer stem cells under hypoxia and serum depletion via decrease in PP2A activity and activation of p38-MAPKAPK2-Hsp27. PLoS ONE. 2012;7:e49605 pubmed publisher
Boisson B, Laplantine E, Prando C, Giliani S, Israelsson E, Xu Z, et al. Immunodeficiency, autoinflammation and amylopectinosis in humans with inherited HOIL-1 and LUBAC deficiency. Nat Immunol. 2012;13:1178-86 pubmed publisher
Ma Z, Hu Y, Jiang G, Hou J, Liu R, Lu Y, et al. Spontaneous generation of germline characteristics in mouse fibrosarcoma cells. Sci Rep. 2012;2:743 pubmed publisher
Rodríguez Jimnez F, Alastrue Agudo A, Erceg S, Stojkovic M, Moreno Manzano V. FM19G11 favors spinal cord injury regeneration and stem cell self-renewal by mitochondrial uncoupling and glucose metabolism induction. Stem Cells. 2012;30:2221-33 pubmed publisher
Claus H, Walberer M, Simard M, Emig B, Muesken S, Rueger M, et al. NG2 and NG2-positive cells delineate focal cerebral infarct demarcation in rats. Neuropathology. 2013;33:30-8 pubmed publisher
Churko J, Shao Q, Gong X, Swoboda K, Bai D, SAMPSON J, et al. Human dermal fibroblasts derived from oculodentodigital dysplasia patients suggest that patients may have wound-healing defects. Hum Mutat. 2011;32:456-66 pubmed publisher
Walberer M, Rueger M, Simard M, Emig B, Jander S, Fink G, et al. Dynamics of neuroinflammation in the macrosphere model of arterio-arterial embolic focal ischemia: an approximation to human stroke patterns. Exp Transl Stroke Med. 2010;2:22 pubmed publisher
Glen A, Evans C, Gan C, Cross S, Hamdy F, Gibbins J, et al. Eight-plex iTRAQ analysis of variant metastatic human prostate cancer cells identifies candidate biomarkers of progression: An exploratory study. Prostate. 2010;70:1313-32 pubmed publisher
Sanchez E, Singru P, Wittmann G, Nouriel S, Barrett P, Fekete C, et al. Contribution of TNF-alpha and nuclear factor-kappaB signaling to type 2 iodothyronine deiodinase activation in the mediobasal hypothalamus after lipopolysaccharide administration. Endocrinology. 2010;151:3827-35 pubmed publisher
Tian R, Wu X, Hagemann T, Sosunov A, Messing A, McKhann G, et al. Alexander disease mutant glial fibrillary acidic protein compromises glutamate transport in astrocytes. J Neuropathol Exp Neurol. 2010;69:335-45 pubmed publisher
Langlois S, Cowan K, Shao Q, Cowan B, Laird D. The tumor-suppressive function of Connexin43 in keratinocytes is mediated in part via interaction with caveolin-1. Cancer Res. 2010;70:4222-32 pubmed publisher
Lee P, Lin H, Jiang S, Lu P, Chou K, Fang H, et al. Mouse kidney progenitor cells accelerate renal regeneration and prolong survival after ischemic injury. Stem Cells. 2010;28:573-84 pubmed publisher
Chi G, Kim M, Kim D, Jiang M, Son Y. Schwann cells differentiated from spheroid-forming cells of rat subcutaneous fat tissue myelinate axons in the spinal cord injury. Exp Neurol. 2010;222:304-17 pubmed publisher
Vince J, Pantaki D, Feltham R, Mace P, Cordier S, Schmukle A, et al. TRAF2 must bind to cellular inhibitors of apoptosis for tumor necrosis factor (tnf) to efficiently activate nf-{kappa}b and to prevent tnf-induced apoptosis. J Biol Chem. 2009;284:35906-15 pubmed publisher
Chaichana K, Guerrero Cazares H, Capilla Gonzalez V, Zamora Berridi G, Achanta P, Gonzalez Perez O, et al. Intra-operatively obtained human tissue: protocols and techniques for the study of neural stem cells. J Neurosci Methods. 2009;180:116-25 pubmed publisher
Mato E, Lucas M, Petriz J, Gomis R, Novials A. Identification of a pancreatic stellate cell population with properties of progenitor cells: new role for stellate cells in the pancreas. Biochem J. 2009;421:181-91 pubmed publisher
Omi H, Okamoto A, Nikaido T, Urashima M, Kawaguchi R, Umehara N, et al. Establishment of an immortalized human extravillous trophoblast cell line by retroviral infection of E6/E7/hTERT and its transcriptional profile during hypoxia and reoxygenation. Int J Mol Med. 2009;23:229-36 pubmed
Zangiacomi V, Balon N, Maddens S, Lapierre V, Tiberghien P, Schlichter R, et al. Cord blood-derived neurons are originated from CD133+/CD34 stem/progenitor cells in a cell-to-cell contact dependent manner. Stem Cells Dev. 2008;17:1005-16 pubmed publisher
Bramanti V, Bronzi D, Tomassoni D, Costa A, Raciti G, Avitabile M, et al. Growth factors and steroid mediated regulation of cytoskeletal protein expression in serum-deprived primary astrocyte cultures. Neurochem Res. 2008;33:2593-600 pubmed publisher
Niclou S, Danzeisen C, Eikesdal H, Wiig H, Brons N, Poli A, et al. A novel eGFP-expressing immunodeficient mouse model to study tumor-host interactions. FASEB J. 2008;22:3120-8 pubmed publisher
Gau B, Chu I, Huang M, Yang K, Chiou S, Fan Y, et al. Transcripts of enriched germ cells responding to heat shock as potential markers for porcine semen quality. Theriogenology. 2008;69:758-66 pubmed publisher
D Alessandro J, Lu K, Fung B, Colman A, Clarke D. Rapid and efficient in vitro generation of pancreatic islet progenitor cells from nonendocrine epithelial cells in the adult human pancreas. Stem Cells Dev. 2007;16:75-89 pubmed
Desilva T, Kinney H, Borenstein N, Trachtenberg F, Irwin N, Volpe J, et al. The glutamate transporter EAAT2 is transiently expressed in developing human cerebral white matter. J Comp Neurol. 2007;501:879-90 pubmed
Ackman J, Ramos R, Sarkisian M, LoTurco J. Citron kinase is required for postnatal neurogenesis in the hippocampus. Dev Neurosci. 2007;29:113-23 pubmed
Ackman J, Siddiqi F, Walikonis R, LoTurco J. Fusion of microglia with pyramidal neurons after retroviral infection. J Neurosci. 2006;26:11413-22 pubmed
Scoles D, Pavelka J, Cass I, Tran H, Baldwin R, Armstrong K, et al. Characterization of CSOC 882, a novel immortalized ovarian cancer cell line expressing EGFR, HER2, and activated AKT. Gynecol Oncol. 2007;104:120-8 pubmed
Flack E, Lindsey M, Squires C, Kaplan B, Stroud R, Clark L, et al. Alterations in cultured myocardial fibroblast function following the development of left ventricular failure. J Mol Cell Cardiol. 2006;40:474-83 pubmed
Schaffer C, Friedman B, Nishimura N, Schroeder L, Tsai P, Ebner F, et al. Two-photon imaging of cortical surface microvessels reveals a robust redistribution in blood flow after vascular occlusion. PLoS Biol. 2006;4:e22 pubmed
Konakci K, Bohle B, Blumer R, Hoetzenecker W, Roth G, Moser B, et al. Alpha-Gal on bioprostheses: xenograft immune response in cardiac surgery. Eur J Clin Invest. 2005;35:17-23 pubmed
product information
Catalog Number :
MAB3400
Subcategory :
Cell Structure
Product Name :
Anti-Vimentin Antibody, clone V9
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P08670
Host Name :
Mouse
Antigen :
Vimentin
Clone :
V9
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-Vimentin Antibody, clone V9
Cross Reactivity :
Bovine;Canine;Chicken;Feline;Human;Monkey;Pig;Rat
Background :
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin.
Immunogen :
Purified vimentin from pig eye lens
Specificity :
Recognizes a 57-60 kDa protein. Shows no cross reactivity with other closely related intermediate filament proteins including desmin and GFAP. In tissue sections, this reagent shows positive reaction on connective tissue, endothelial cells, vascular smooth muscle cells and astrocytes. In tissue culture, typical intermediate filament profiles are seen in fibroblasts and other cell types, which contain vimentin
Package Size :
40 μg
Uses :
Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting
Storage :
Stable for 1 year at 2-8ºC from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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