Collagen Type I Antibody, clone 5D8-G9 | EMD Millipore MAB3391 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Collagen Type I Antibody, clone 5D8-G9

catalog :

MAB3391

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5D8-G9

The same clone is also sold as:

Invitrogen: MA1-141

reactivity :

human, bovine

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

citations: 38

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:1000	Patel P, Khan N, Rani M, Gupta D, Jameel S. The expression of HIV-1 Vpu in monocytes causes increased secretion of TGF-? that activates profibrogenic genes in hepatic stellate cells. PLoS ONE. 2014;9:e88934 pubmed publisher
immunohistochemistry; bovine; 10 ug/ml	Farrell M, Fisher M, HUANG A, Shin J, Farrell K, Mauck R. Functional properties of bone marrow-derived MSC-based engineered cartilage are unstable with very long-term in vitro culture. J Biomech. 2014;47:2173-82 pubmed publisher
flow cytometry; human; 1:100 immunocytochemistry; human; 1:100	Kidwai F, Movahednia M, Iqbal K, Jokhun D, Cao T, Fawzy A. Human embryonic stem cell differentiation into odontoblastic lineage: an in vitro study. Int Endod J. 2014;47:346-55 pubmed publisher
immunohistochemistry; human western blot; human	Choi W, Jeon H, Chung Y, Lim J, Shin D, Kim J, et al. Isolation and characterization of novel, highly proliferative human CD34/CD73-double-positive testis-derived stem cells for cell therapy. Stem Cells Dev. 2013;22:2158-73 pubmed publisher
	Kim M, Steinberg D, Burdick J, Mauck R. Extracellular vesicles mediate improved functional outcomes in engineered cartilage produced from MSC/chondrocyte cocultures. Proc Natl Acad Sci U S A. 2019;116:1569-1578 pubmed publisher
	Sinha R, Hoon M, Baudin J, Okawa H, Wong R, Rieke F. Cellular and Circuit Mechanisms Shaping the Perceptual Properties of the Primate Fovea. Cell. 2017;168:413-426.e12 pubmed publisher
	Yang L, Geng Z, Nickel T, Johnson C, Gao L, Dutton J, et al. Differentiation of Human Induced-Pluripotent Stem Cells into Smooth-Muscle Cells: Two Novel Protocols. PLoS ONE. 2016;11:e0147155 pubmed publisher
	BourdÃ³n Santoyo M, QuiÃ±ones Uriostegui I, MartÃnez LÃ³pez V, SÃ¡nchez ArÃ©valo F, Alessi Montero A, Velasquillo C, et al. Preliminary study of an in vitro development of new tissue applying mechanical stimulation with a bioreactor as an alternative for ligament reconstruction. Rev Invest Clin. 2014;66 Suppl 1:S100-10 pubmed
	Sharma M, Tiwari A, Sharma S, Bansal R, Gupta V, Gupta A, et al. Pathological vitreous causes cell line-derived (but not donor-derived) retinal pigment epithelial cells to display proliferative vitreoretinopathy-like features in culture. Clin Exp Ophthalmol. 2014;42:745-60 pubmed publisher
	Bastiaans J, van Meurs J, van Holten Neelen C, Nagtzaam N, van Hagen P, Chambers R, et al. Thrombin induces epithelial-mesenchymal transition and collagen production by retinal pigment epithelial cells via autocrine PDGF-receptor signaling. Invest Ophthalmol Vis Sci. 2013;54:8306-14 pubmed publisher
	Nischalke H, Berger C, Lutz P, Langhans B, Wolter F, Eisenhardt M, et al. Influence of the CXCL1 rs4074 A allele on alcohol induced cirrhosis and HCC in patients of European descent. PLoS ONE. 2013;8:e80848 pubmed publisher
	Marmotti A, Bruzzone M, Bonasia D, Castoldi F, Von Degerfeld M, Bignardi C, et al. Autologous cartilage fragments in a composite scaffold for one stage osteochondral repair in a goat model. Eur Cell Mater. 2013;26:15-31; discussion 31-2 pubmed
	Viola I, Palamà I, Coluccia A, Biasiucci M, Dozza B, Lucarelli E, et al. Physiological formation of fluorescent and conductive protein microfibers in live fibroblasts upon spontaneous uptake of biocompatible fluorophores. Integr Biol (Camb). 2013;5:1057-66 pubmed publisher
	Behl B, Papageorgiou I, Brown C, Hall R, Tipper J, Fisher J, et al. Biological effects of cobalt-chromium nanoparticles and ions on dural fibroblasts and dural epithelial cells. Biomaterials. 2013;34:3547-58 pubmed publisher
	Guidotti S, Facchini A, Platano D, Olivotto E, Minguzzi M, Trisolino G, et al. Enhanced osteoblastogenesis of adipose-derived stem cells on spermine delivery via ?-catenin activation. Stem Cells Dev. 2013;22:1588-601 pubmed publisher
	Cheng T, Yang C, Weber N, Kim H, Kuo A. Fibroblast growth factor 2 enhances the kinetics of mesenchymal stem cell chondrogenesis. Biochem Biophys Res Commun. 2012;426:544-50 pubmed publisher
	HUANG A, Baker B, Ateshian G, Mauck R. Sliding contact loading enhances the tensile properties of mesenchymal stem cell-seeded hydrogels. Eur Cell Mater. 2012;24:29-45 pubmed
	Saliken D, Mulet Sierra A, Jomha N, Adesida A. Decreased hypertrophic differentiation accompanies enhanced matrix formation in co-cultures of outer meniscus cells with bone marrow mesenchymal stromal cells. Arthritis Res Ther. 2012;14:R153 pubmed publisher
	Adhikari A, Glassey E, Dunn A. Conformational dynamics accompanying the proteolytic degradation of trimeric collagen I by collagenases. J Am Chem Soc. 2012;134:13259-65 pubmed publisher
	Adesida A, Mulet Sierra A, Laouar L, Jomha N. Oxygen tension is a determinant of the matrix-forming phenotype of cultured human meniscal fibrochondrocytes. PLoS ONE. 2012;7:e39339 pubmed publisher
	Bucchieri F, Fucarino A, Marino Gammazza A, Pitruzzella A, Marciano V, Paderni C, et al. Medium-term culture of normal human oral mucosa: a novel three-dimensional model to study the effectiveness of drugs administration. Curr Pharm Des. 2012;18:5421-30 pubmed
	Matthies N, Mulet Sierra A, Jomha N, Adesida A. Matrix formation is enhanced in co-cultures of human meniscus cells with bone marrow stromal cells. J Tissue Eng Regen Med. 2013;7:965-73 pubmed publisher
	Adesida A, Mulet Sierra A, Jomha N. Hypoxia mediated isolation and expansion enhances the chondrogenic capacity of bone marrow mesenchymal stromal cells. Stem Cell Res Ther. 2012;3:9 pubmed publisher
	Kim J, Choi I, Lee B, Park J, Kim J, Jeong J, et al. Neuregulin induces CTGF expression in hypertrophic scarring fibroblasts. Mol Cell Biochem. 2012;365:181-9 pubmed publisher
	Gu W, Liu W, Yang X, Yuan X, Tian Y, Meng R, et al. Cutis laxa: analysis of metalloproteinases and extracellular matrix expression by immunohistochemistry and histochemistry. Eur J Dermatol. 2011;21:717-21 pubmed publisher
	Mi H, Lee M, Fu E, Chow L, Lin C. Highly efficient multipotent differentiation of human periodontal ligament fibroblasts induced by combined BMP4 and hTERT gene transfer. Gene Ther. 2011;18:452-61 pubmed publisher
	Waterman R, Tomchuck S, Henkle S, Betancourt A. A new mesenchymal stem cell (MSC) paradigm: polarization into a pro-inflammatory MSC1 or an Immunosuppressive MSC2 phenotype. PLoS ONE. 2010;5:e10088 pubmed publisher
	HUANG A, Stein A, Mauck R. Evaluation of the complex transcriptional topography of mesenchymal stem cell chondrogenesis for cartilage tissue engineering. Tissue Eng Part A. 2010;16:2699-708 pubmed publisher
	Erickson I, Huang A, Sengupta S, Kestle S, Burdick J, Mauck R. Macromer density influences mesenchymal stem cell chondrogenesis and maturation in photocrosslinked hyaluronic acid hydrogels. Osteoarthritis Cartilage. 2009;17:1639-48 pubmed publisher
	Gemmiti C, Guldberg R. Shear stress magnitude and duration modulates matrix composition and tensile mechanical properties in engineered cartilaginous tissue. Biotechnol Bioeng. 2009;104:809-20 pubmed publisher
	HUANG A, Stein A, Tuan R, Mauck R. Transient exposure to transforming growth factor beta 3 improves the mechanical properties of mesenchymal stem cell-laden cartilage constructs in a density-dependent manner. Tissue Eng Part A. 2009;15:3461-72 pubmed publisher
	Mehlhorn A, Zwingmann J, Finkenzeller G, Niemeyer P, Dauner M, Stark B, et al. Chondrogenesis of adipose-derived adult stem cells in a poly-lactide-co-glycolide scaffold. Tissue Eng Part A. 2009;15:1159-67 pubmed publisher
	Kelly T, Ng K, Ateshian G, Hung C. Analysis of radial variations in material properties and matrix composition of chondrocyte-seeded agarose hydrogel constructs. Osteoarthritis Cartilage. 2009;17:73-82 pubmed publisher
	Huang A, Yeger McKeever M, Stein A, Mauck R. Tensile properties of engineered cartilage formed from chondrocyte- and MSC-laden hydrogels. Osteoarthritis Cartilage. 2008;16:1074-82 pubmed publisher
	Gavish L, Perez L, Gertz S. Low-level laser irradiation modulates matrix metalloproteinase activity and gene expression in porcine aortic smooth muscle cells. Lasers Surg Med. 2006;38:779-86 pubmed
	Gemmiti C, Guldberg R. Fluid flow increases type II collagen deposition and tensile mechanical properties in bioreactor-grown tissue-engineered cartilage. Tissue Eng. 2006;12:469-79 pubmed
	Jiang J, Nicoll S, Lu H. Co-culture of osteoblasts and chondrocytes modulates cellular differentiation in vitro. Biochem Biophys Res Commun. 2005;338:762-70 pubmed
	Grigolo B, Roseti L, De Franceschi L, Piacentini A, Cattini L, Manfredini M, et al. Molecular and immunohistological characterization of human cartilage two years following autologous cell transplantation. J Bone Joint Surg Am. 2005;87:46-57 pubmed

product information

Catalog Number :

MAB3391

Subcategory :

Cell Structure

Product Name :

Anti-Collagen Type I Antibody, clone 5D8-G9

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P02452

Host Name :

Mouse

Antigen :

Collagen Type I

Clone :

5D8-G9

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Collagen Type I Antibody, clone 5D8-G9

Cross Reactivity :

Bovine;Canine;Human;Pig;Sheep

Background :

Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Specificity :

Collagen Type I Antibody is a monoclonal IgG1 antibody which binds an epitope near the C-terminal of Type I Collagen. Collagen Type I Antibody displays a high affinity for human, bovine and ovine Type I Collagens. There is no evidence for cross reactivity with Collagen Types III, V and VI or connective tissue protein. Antibody reacts only with native, non-denatured Collagen I

Package Size :

100 µg

Uses :

ELISA;Flow Cytometry;Western Blotting;Immunohistochemistry

Storage :

Collagen Type I Antibody is shipped, in liquid form, at ambient temperature. This material is stable for up to 6 months when stored at 2-8°C. WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.