MMP-2 Antibody, a.a. 468-483 hMMP2, clone 42-5D11 | EMD Millipore MAB3308 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MMP-2 Antibody, a.a. 468-483 hMMP2, clone 42-5D11

catalog :

MAB3308

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

42-5D11

The same clone is also sold as:

LifeSpan Biosciences: LS-C85320

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 56

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 3	Yang Y, Sun Y, Acott T, Keller K. Effects of induction and inhibition of matrix cross-linking on remodeling of the aqueous outflow resistance by ocular trabecular meshwork cells. Sci Rep. 2016;6:30505 pubmed publisher
immunohistochemistry - frozen section; rat; 1:500 western blot; rat; 1:1000	Guimaraes D, Rizzi E, Ceron C, Martins Oliveira A, Gerlach R, Shiva S, et al. Atorvastatin and sildenafil decrease vascular TGF-β levels and MMP-2 activity and ameliorate arterial remodeling in a model of renovascular hypertension. Redox Biol. 2015;6:386-395 pubmed publisher
western blot; human	Zhao H, Agazie Y. Inhibition of SHP2 in basal-like and triple-negative breast cells induces basal-to-luminal transition, hormone dependency, and sensitivity to anti-hormone treatment. BMC Cancer. 2015;15:109 pubmed publisher
western blot; mouse; 1:1000	Itman C, Bielanowicz A, Goh H, Lee Q, Fulcher A, Moody S, et al. Murine Inhibin Î±-Subunit Haploinsufficiency Causes Transient Abnormalities in Prepubertal Testis Development Followed by Adult Testicular Decline. Endocrinology. 2015;156:2254-68 pubmed publisher
western blot; rat; loading ...; fig 3c	Nishihara T, Remacle A, Angert M, Shubayev I, Shiryaev S, Liu H, et al. Matrix metalloproteinase-14 both sheds cell surface neuronal glial antigen 2 (NG2) proteoglycan on macrophages and governs the response to peripheral nerve injury. J Biol Chem. 2015;290:3693-707 pubmed publisher
immunohistochemistry; human; fig 3 western blot; human; fig 3	Aga M, Bradley J, Wanchu R, Yang Y, Acott T, Keller K. Differential effects of caveolin-1 and -2 knockdown on aqueous outflow and altered extracellular matrix turnover in caveolin-silenced trabecular meshwork cells. Invest Ophthalmol Vis Sci. 2014;55:5497-509 pubmed publisher
immunohistochemistry - frozen section; rat	Guerra C, Carla Lara Pereira Y, Issa J, Luiz K, Guimarães E, Gerlach R, et al. Histological, histochemical, and protein changes after induced malocclusion by occlusion alteration of Wistar rats. Biomed Res Int. 2014;2014:563463 pubmed publisher
western blot; rat	Kopaliani I, Martin M, Zatschler B, Bortlik K, Müller B, Deussen A. Cell-specific and endothelium-dependent regulations of matrix metalloproteinase-2 in rat aorta. Basic Res Cardiol. 2014;109:419 pubmed publisher
western blot; rat	Akkad H, Corpeno R, Larsson L. Masseter muscle myofibrillar protein synthesis and degradation in an experimental critical illness myopathy model. PLoS ONE. 2014;9:e92622 pubmed publisher
immunocytochemistry; mouse; fig 2	Hughes B, Fan X, Cho W, Schulz R. MMP-2 is localized to the mitochondria-associated membrane of the heart. Am J Physiol Heart Circ Physiol. 2014;306:H764-70 pubmed publisher
	Vu H, Hoang T, Kim J. All-Trans Retinoic Acid Enhances Matrix Metalloproteinase 2 Expression and Secretion in Human Myeloid Leukemia THP-1 Cells. Biomed Res Int. 2018;2018:5971080 pubmed publisher
	Liu B, Cui J, Sun J, Li J, Han X, Guo J, et al. Immunolocalization of MMP9 and MMP2 in osteolytic metastasis originating from MDA-MB-231 human breast cancer cells. Mol Med Rep. 2016;14:1099-106 pubmed publisher
	Liaw N, Fox J, Siddiqui A, Meng H, Kolega J. Endothelial nitric oxide synthase and superoxide mediate hemodynamic initiation of intracranial aneurysms. PLoS ONE. 2014;9:e101721 pubmed publisher
	Mao S, Wang Y, Zhang M, Hinek A. Phytoestrogen, tanshinone IIA diminishes collagen deposition and stimulates new elastogenesis in cultures of human cardiac fibroblasts. Exp Cell Res. 2014;323:189-97 pubmed publisher
	Bencsik P, Pálóczi J, Kocsis G, Pipis J, Belecz I, Varga Z, et al. Moderate inhibition of myocardial matrix metalloproteinase-2 by ilomastat is cardioprotective. Pharmacol Res. 2014;80:36-42 pubmed publisher
	Nascimento G, Rizzi E, Gerlach R, Leite Panissi C. Expression of MMP-2 and MMP-9 in the rat trigeminal ganglion during the development of temporomandibular joint inflammation. Braz J Med Biol Res. 2013;46:956-967 pubmed
	Antonio R, Ceron C, Rizzi E, Coelho E, Tanus Santos J, Gerlach R. Antioxidant effect of doxycycline decreases MMP activity and blood pressure in SHR. Mol Cell Biochem. 2014;386:99-105 pubmed publisher
	Vuckovic A, Herber Jonat S, Flemmer A, Roubliova X, Jani J. Alveolarization genes modulated by fetal tracheal occlusion in the rabbit model for congenital diaphragmatic hernia: a randomized study. PLoS ONE. 2013;8:e69210 pubmed publisher
	Ceron C, Rizzi E, Guimaraes D, Martins Oliveira A, Gerlach R, Tanus Santos J. Nebivolol attenuates prooxidant and profibrotic mechanisms involving TGF-? and MMPs, and decreases vascular remodeling in renovascular hypertension. Free Radic Biol Med. 2013;65:47-56 pubmed publisher
	Sevin G, Ozsarlak Sozer G, Keles D, Gokce G, Reel B, Ozgur H, et al. Taurine inhibits increased MMP-2 expression in a model of oxidative stress induced by glutathione depletion in rabbit heart. Eur J Pharmacol. 2013;706:98-106 pubmed publisher
	Renna N, Diez E, Lembo C, Miatello R. Role of Cox-2 in vascular inflammation: an experimental model of metabolic syndrome. Mediators Inflamm. 2013;2013:513251 pubmed publisher
	Ferrer M, Rodriguez H, Zara L, Yu Y, Xu W, Oko R. MMP2 and acrosin are major proteinases associated with the inner acrosomal membrane and may cooperate in sperm penetration of the zona pellucida during fertilization. Cell Tissue Res. 2012;349:881-95 pubmed publisher
	Cardeal L, Boccardo E, Termini L, Rabachini T, Andreoli M, Di Loreto C, et al. HPV16 oncoproteins induce MMPs/RECK-TIMP-2 imbalance in primary keratinocytes: possible implications in cervical carcinogenesis. PLoS ONE. 2012;7:e33585 pubmed publisher
	GonÃ§alves A, Meschiari C, Stetler Stevenson W, Nonato M, Alves C, Espreafico E, et al. Expression of soluble and functional full-length human matrix metalloproteinase-2 in Escherichia coli. J Biotechnol. 2012;157:20-4 pubmed publisher
	Solini A, Rossi C, Santini E, Madec S, Salvati A, Ferrannini E. Angiotensin-II and rosuvastatin influence matrix remodeling in human mesangial cells via metalloproteinase modulation. J Hypertens. 2011;29:1930-9 pubmed publisher
	Fang J, Zhou H, Zeng C, Yang J, Liu Y, Huang X, et al. MicroRNA-29b suppresses tumor angiogenesis, invasion, and metastasis by regulating matrix metalloproteinase 2 expression. Hepatology. 2011;54:1729-40 pubmed publisher
	Cau S, Guimarães D, Rizzi E, Ceron C, Souza L, Tirapelli C, et al. Pyrrolidine dithiocarbamate down-regulates vascular matrix metalloproteinases and ameliorates vascular dysfunction and remodelling in renovascular hypertension. Br J Pharmacol. 2011;164:372-81 pubmed publisher
	Setz C, Brand Y, Radojevic V, Hanusek C, Mullen P, Levano S, et al. Matrix metalloproteinases 2 and 9 in the cochlea: expression and activity after aminoglycoside exposition. Neuroscience. 2011;181:28-39 pubmed publisher
	Guimaraes D, Rizzi E, Ceron C, Oliveira A, Oliveira D, Castro M, et al. Doxycycline dose-dependently inhibits MMP-2-mediated vascular changes in 2K1C hypertension. Basic Clin Pharmacol Toxicol. 2011;108:318-25 pubmed publisher
	Marçal D, Rizzi E, Martins Oliveira A, Ceron C, Guimaraes D, Gerlach R, et al. Comparative study on antioxidant effects and vascular matrix metalloproteinase-2 downregulation by dihydropyridines in renovascular hypertension. Naunyn Schmiedebergs Arch Pharmacol. 2011;383:35-44 pubmed publisher
	Sen E, Balikoglu Yilmaz M, Bozdag Pehlivan S, Sungu N, Aksakal F, Altinok A, et al. Effect of doxycycline on postoperative scarring after trabeculectomy in an experimental rabbit model. J Ocul Pharmacol Ther. 2010;26:399-406 pubmed publisher
	Lee Y, Lee N, Bhattarai G, Yun J, Kim T, Jhee E, et al. PPARgamma inhibits inflammatory reaction in oxidative stress induced human diploid fibloblast. Cell Biochem Funct. 2010;28:490-6 pubmed publisher
	Rizzi E, Castro M, Prado C, Silva C, Fazan R, Rossi M, et al. Matrix metalloproteinase inhibition improves cardiac dysfunction and remodeling in 2-kidney, 1-clip hypertension. J Card Fail. 2010;16:599-608 pubmed publisher
	Choi J, Park G, Na E, Wi H, Lee S, Lee J. Molecular changes following topical photodynamic therapy using methyl aminolaevulinate in mouse skin. J Dermatol Sci. 2010;58:198-203 pubmed publisher
	Ceron C, Castro M, Rizzi E, Montenegro M, Fontana V, Salgado M, et al. Spironolactone and hydrochlorothiazide exert antioxidant effects and reduce vascular matrix metalloproteinase-2 activity and expression in a model of renovascular hypertension. Br J Pharmacol. 2010;160:77-87 pubmed publisher
	Paula Silva F, da Silva L, Kapila Y. Matrix metalloproteinase expression in teeth with apical periodontitis is differentially modulated by the modality of root canal treatment. J Endod. 2010;36:231-7 pubmed publisher
	Castro M, Rizzi E, Prado C, Rossi M, Tanus Santos J, Gerlach R. Imbalance between matrix metalloproteinases and tissue inhibitor of metalloproteinases in hypertensive vascular remodeling. Matrix Biol. 2010;29:194-201 pubmed publisher
	Lahat G, Tuvin D, Wei C, Wang W, Pollock R, Anaya D, et al. Molecular prognosticators of complex karyotype soft tissue sarcoma outcome: a tissue microarray-based study. Ann Oncol. 2010;21:1112-20 pubmed publisher
	Schenke Layland K, Xie J, Magnusson M, Angelis E, Li X, Wu K, et al. Lymphocytic infiltration leads to degradation of lacrimal gland extracellular matrix structures in NOD mice exhibiting a Sjögren's syndrome-like exocrinopathy. Exp Eye Res. 2010;90:223-37 pubmed publisher
	Cena J, Lalu M, Cho W, Chow A, Bagdan M, Daniel E, et al. Inhibition of matrix metalloproteinase activity in vivo protects against vascular hyporeactivity in endotoxemia. Am J Physiol Heart Circ Physiol. 2010;298:H45-51 pubmed publisher
	Dooley J, Abdel Latif D, St Laurent C, Puttagunta L, Befus D, Lacy P. Regulation of inflammation by Rac2 in immune complex-mediated acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2009;297:L1091-102 pubmed publisher
	Goetzenich A, Hatam N, Zernecke A, Weber C, Czarnotta T, Autschbach R, et al. Alteration of matrix metalloproteinases in selective left ventricular adriamycin-induced cardiomyopathy in the pig. J Heart Lung Transplant. 2009;28:1087-93 pubmed publisher
	de Paula Silva F, D Silva N, da Silva L, Kapila Y. High matrix metalloproteinase activity is a hallmark of periapical granulomas. J Endod. 2009;35:1234-42 pubmed publisher
	Akkaya P, Onalan G, Haberal N, Bayraktar N, Mulayim B, Zeyneloglu H. Doxycycline causes regression of endometriotic implants: a rat model. Hum Reprod. 2009;24:1900-8 pubmed publisher
	Castro M, Rizzi E, Rodrigues G, Ceron C, Bendhack L, Gerlach R, et al. Antioxidant treatment reduces matrix metalloproteinase-2-induced vascular changes in renovascular hypertension. Free Radic Biol Med. 2009;46:1298-307 pubmed publisher
	Porto I, Rocha L, Rossi M, Gerlach R. In situ zymography and immunolabeling in fixed and decalcified craniofacial tissues. J Histochem Cytochem. 2009;57:615-22 pubmed publisher
	Castro M, Rizzi E, Figueiredo Lopes L, Fernandes K, Bendhack L, Pitol D, et al. Metalloproteinase inhibition ameliorates hypertension and prevents vascular dysfunction and remodeling in renovascular hypertensive rats. Atherosclerosis. 2008;198:320-31 pubmed
	Cho W, Chow A, Schulz R, Daniel E. Matrix metalloproteinase-2, caveolins, focal adhesion kinase and c-Kit in cells of the mouse myocardium. J Cell Mol Med. 2007;11:1069-86 pubmed
	Watts S, Thakali K, Smark C, Rondelli C, Fink G. Big ET-1 processing into vasoactive peptides in arteries and veins. Vascul Pharmacol. 2007;47:302-12 pubmed
	Lluri G, Langlois G, Soloway P, Jaworski D. Tissue inhibitor of metalloproteinase-2 (TIMP-2) regulates myogenesis and beta1 integrin expression in vitro. Exp Cell Res. 2008;314:11-24 pubmed
	Veiseh M, Gabikian P, Bahrami S, Veiseh O, Zhang M, Hackman R, et al. Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci. Cancer Res. 2007;67:6882-8 pubmed
	Frich L, Bjørnland K, Pettersen S, Clausen O, Gladhaug I. Increased activity of matrix metalloproteinase 2 and 9 after hepatic radiofrequency ablation. J Surg Res. 2006;135:297-304 pubmed
	Liu M, Akle V, Zheng W, Kitlen J, O Steen B, Larner S, et al. Extensive degradation of myelin basic protein isoforms by calpain following traumatic brain injury. J Neurochem. 2006;98:700-12 pubmed
	Lalu M, Cena J, Chowdhury R, Lam A, Schulz R. Matrix metalloproteinases contribute to endotoxin and interleukin-1beta induced vascular dysfunction. Br J Pharmacol. 2006;149:31-42 pubmed
	Lalu M, Pasini E, Schulze C, Ferrari Vivaldi M, Ferrari Vivaldi G, Bachetti T, et al. Ischaemia-reperfusion injury activates matrix metalloproteinases in the human heart. Eur Heart J. 2005;26:27-35 pubmed
	Qiu W, Bai S, Zhao M, Wu X, Zhao Y, Sang Q, et al. Spatio-temporal expression of matrix metalloproteinase-26 in human placental trophoblasts and fetal red cells during normal placentation. Biol Reprod. 2005;72:954-9 pubmed

product information

Catalog Number :

MAB3308

Subcategory :

Cell Structure

Product Name :

Anti-MMP-2 Antibody, a.a. 468-483 hMMP2, clone 42-5D11

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P08253

Host Name :

Mouse

Antigen :

MMP-2

Clone :

42-5D11

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-MMP-2 Antibody, a.a. 468-483 hMMP2, clone 42-5D11

Cross Reactivity :

Bovine;Horse;Human;Mouse;Pig;Rat;Rabbit

Background :

The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis. MMP2, also known as Gelatinase A, is a type IV collagenase that specifically cleaves type IV collagen, the major structural component of basement membranes. The metastatic potential of tumor cells has been found to correlate with the activity of this enzyme.

ALT Names :

Gelatinase A; 72 kDa Type IV Collagenase

Immunogen :

Synthetic oligopeptide corresponding to amino acid residue 468-483 (VTPRDKPMGPLLVATF) of human matrix metalloproteinase 2 (human MMP-2, gelatinase A).

Specificity :

Specifically reacts with precursor and active forms of human MMP-2. Does not cross react with human MMP-1, -3, -9, or -13. Also reacts with rat, mouse, and bovine MMP2, other species not tested.

Package Size :

100 μg

Uses :

ELISA;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.