DNA Antibody, single stranded specific, clone F7-26 | EMD Millipore MAB3299 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

DNA Antibody, single stranded specific, clone F7-26

catalog :

MAB3299

quantity :

50 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

F7-26

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

citations: 26

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human	Liu F, Lou J, Zhao D, Li W, Zhao Y, Sun X, et al. Dysferlinopathy: mitochondrial abnormalities in human skeletal muscle. Int J Neurosci. 2016;126:499-509 pubmed publisher
	Guillermo Lagae R, Deep G, Ting H, Agarwal C, Agarwal R. Silibinin enhances the repair of ultraviolet B-induced DNA damage by activating p53-dependent nucleotide excision repair mechanism in human dermal fibroblasts. Oncotarget. 2015;6:39594-606 pubmed publisher
	Jackson K, Christiansen V, Yadav V, Silasi Mansat R, Lupu F, Awasthi V, et al. Suppression of tumor growth in mice by rationally designed pseudopeptide inhibitors of fibroblast activation protein and prolyl oligopeptidase. Neoplasia. 2015;17:43-54 pubmed publisher
	Chang A, Ulrich A, Suliman H, Piantadosi C. Redox regulation of mitophagy in the lung during murine Staphylococcus aureus sepsis. Free Radic Biol Med. 2015;78:179-89 pubmed publisher
	Golbidi S, Botta A, Gottfred S, Nusrat A, Laher I, Ghosh S. Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercise. Br J Pharmacol. 2014;171:5345-60 pubmed publisher
	Zoppis S, Muciaccia B, D Alessio A, Ziparo E, Vecchiotti C, Filippini A. DNA fingerprinting secondary transfer from different skin areas: Morphological and genetic studies. Forensic Sci Int Genet. 2014;11:137-43 pubmed publisher
	Iglesias Guimarais V, Gil Guiñón E, Sánchez Osuna M, Casanelles E, García Belinchón M, Comella J, et al. Chromatin collapse during caspase-dependent apoptotic cell death requires DNA fragmentation factor, 40-kDa subunit-/caspase-activated deoxyribonuclease-mediated 3'-OH single-strand DNA breaks. J Biol Chem. 2013;288:9200-15 pubmed publisher
	Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, et al. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis. Stem Cells. 2013;31:146-55 pubmed publisher
	Nakagawa S, Nishihara K, Inui K, Masuda S. Involvement of autophagy in the pharmacological effects of the mTOR inhibitor everolimus in acute kidney injury. Eur J Pharmacol. 2012;696:143-54 pubmed publisher
	Fung S, Xi M, Zhang J, Sampogna S, Chase M. Apnea produces excitotoxic hippocampal synapses and neuronal apoptosis. Exp Neurol. 2012;238:107-13 pubmed publisher
	Salvaing J, Aguirre Lavin T, Boulesteix C, Lehmann G, Debey P, Beaujean N. 5-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote. PLoS ONE. 2012;7:e38156 pubmed publisher
	Kobayashi F, Watanabe E, Nakagawa Y, Yamanishi S, Norose Y, Fukunaga Y, et al. Production of autoantibodies by murine B-1a cells stimulated with Helicobacter pylori urease through toll-like receptor 2 signaling. Infect Immun. 2011;79:4791-801 pubmed publisher
	Douglas R, Ryu J, Kanaan A, Del Carmen Rivero M, Dugan L, Haddad G, et al. Neuronal death during combined intermittent hypoxia/hypercapnia is due to mitochondrial dysfunction. Am J Physiol Cell Physiol. 2010;298:C1594-602 pubmed publisher
	Andrieu C, Taieb D, Baylot V, Ettinger S, Soubeyran P, de Thonel A, et al. Heat shock protein 27 confers resistance to androgen ablation and chemotherapy in prostate cancer cells through eIF4E. Oncogene. 2010;29:1883-96 pubmed publisher
	Fung S, Xi M, Zhang J, Yamuy J, Sampogna S, Tsai K, et al. Eszopiclone prevents excitotoxicity and neurodegeneration in the hippocampus induced by experimental apnea. Sleep. 2009;32:1593-601 pubmed
	Ahmed S, Silverman M, Marotte H, Kwan K, Matuszczak N, Koch A. Down-regulation of myeloid cell leukemia 1 by epigallocatechin-3-gallate sensitizes rheumatoid arthritis synovial fibroblasts to tumor necrosis factor alpha-induced apoptosis. Arthritis Rheum. 2009;60:1282-93 pubmed publisher
	Dang A, Kim H. Chondrocyte apoptosis after simulated intraarticular fracture: a comparison of histologic detection methods. Clin Orthop Relat Res. 2009;467:1877-84 pubmed publisher
	Machaalani R, Waters K. Neuronal cell death in the Sudden Infant Death Syndrome brainstem and associations with risk factors. Brain. 2008;131:218-28 pubmed
	Ulker O, Genc S, Ates H, Durak H, Atabey N. 99mTc-HMPAO labelling inhibits cell motility and cell proliferation and induces apoptosis of NC-NC cells. Mutat Res. 2007;631:69-76 pubmed
	Hamberg A, Ringler S, Krakowka S. A novel method for the detection of porcine circovirus type 2 replicative double stranded viral DNA and nonreplicative single stranded viral DNA in tissue sections. J Vet Diagn Invest. 2007;19:135-41 pubmed
	Rocchi P, Jugpal P, So A, Sinneman S, Ettinger S, Fazli L, et al. Small interference RNA targeting heat-shock protein 27 inhibits the growth of prostatic cell lines and induces apoptosis via caspase-3 activation in vitro. BJU Int. 2006;98:1082-9 pubmed
	Ito Y, Shibata M, Kusakabe K, Otsuki Y. Method of specific detection of apoptosis using formamide-induced DNA denaturation assay. J Histochem Cytochem. 2006;54:683-92 pubmed
	Rocchi P, Beraldi E, Ettinger S, Fazli L, Vessella R, Nelson C, et al. Increased Hsp27 after androgen ablation facilitates androgen-independent progression in prostate cancer via signal transducers and activators of transcription 3-mediated suppression of apoptosis. Cancer Res. 2005;65:11083-93 pubmed
	Bartlett J, Dwyer S, Beniash E, Skobe Z, Payne Ferreira T. Fluorosis: a new model and new insights. J Dent Res. 2005;84:832-6 pubmed
	Hodge S, Hodge G, Holmes M, Reynolds P. Increased peripheral blood T-cell apoptosis and decreased Bcl-2 in chronic obstructive pulmonary disease. Immunol Cell Biol. 2005;83:160-6 pubmed
	Orlowski R, Dees E. The role of the ubiquitination-proteasome pathway in breast cancer: applying drugs that affect the ubiquitin-proteasome pathway to the therapy of breast cancer. Breast Cancer Res. 2003;5:1-7 pubmed

product information

Catalog Number :

MAB3299

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-DNA Antibody, single stranded specific, clone F7-26

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

DNA

Clone :

F7-26

Conjugate :

Purified

Isotype :

IgM

Product Description :

Anti-DNA Antibody, single stranded specific, clone F7-26

Cross Reactivity :

All

Background :

Apoptosis is a genetically and biochemically regulated mechanism of programmed cell death that plays an important role in a variety of human diseases including cancer, immune disorders, and neurologic, cardiovascular and infectious diseases. Decreased stability of apoptotic DNA toward thermal denaturation is induced by the proteolysis of DNA-bound proteins during the execution of apoptosis. This conclusion is supported by the binding of anti-ssDNA monoclonal antibodies to non-apoptotic cells treated with proteinase K before heating and by prevention of DNA denaturation and MAb reactivity in apoptotic nuclei reconstituted with histones (Frankfurt et al. 1996, 1997). Thus, staining of cell suspensions and tissue sections with such antibodies following a heat treatment, which induces DNA denaturation in situ only in apoptotic nuclei, is a specific and sensitive method for the detection of apoptotic cells. CHEMICON MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types.

Immunogen :

F7-26 was generated by the immunization of mice with calf thymus single-stranded DNA.

Specificity :

Specifically reactive with single-stranded DNA and is ideal for the detection of apoptotic cells in suspension or in tissue sections. The antibody does not recognize DNA in double-stranded conformations. MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types. Antibody reacts specifically with deoxycytidine and requires stretch of ssDNA of at least 25-30 bases in length for the binding. Importantly, in contrast with the TUNEL method, monoclonal antibodies to ssDNA are specific for apoptotic cell death and do not detect necrotic cells.

Package Size :

50 μg

Uses :

Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin)

Storage :

Maintain frozen at -80°C in undiluted aliquots for up to 12 months after date of purchase.