CNPase Antibody, clone 11-5B | EMD Millipore MAB326R product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

CNPase Antibody, clone 11-5B

catalog :

MAB326R

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

11-5B

The same clone is also sold as:

LifeSpan Biosciences: LS-C88110
Abcam: ab6319
MilliporeSigma: C5922

reactivity :

human, mouse, rat, zebrafish , domestic sheep, rhesus macaque

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 44

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; zebrafish ; 1:200; fig 3	Hui S, Nag T, Ghosh S. Characterization of Proliferating Neural Progenitors after Spinal Cord Injury in Adult Zebrafish. PLoS ONE. 2015;10:e0143595 pubmed publisher
immunocytochemistry; rat; 1:500; fig 5	Rabenstein M, Hucklenbroich J, Willuweit A, Ladwig A, Fink G, Schroeter M, et al. Osteopontin mediates survival, proliferation and migration of neural stem cells through the chemokine receptor CXCR4. Stem Cell Res Ther. 2015;6:99 pubmed publisher
immunohistochemistry - paraffin section; mouse; fig 6	Yufune S, Satoh Y, Takamatsu I, Ohta H, Kobayashi Y, Takaenoki Y, et al. Transient Blockade of ERK Phosphorylation in the Critical Period Causes Autistic Phenotypes as an Adult in Mice. Sci Rep. 2015;5:10252 pubmed publisher
immunohistochemistry - paraffin section; domestic sheep; 1:500	Kitanishi R, Matsuda T, Watanabe S, Saito M, Hanita T, Watanabe T, et al. Cerebral ischemia or intrauterine inflammation promotes differentiation of oligodendroglial precursors in preterm ovine fetuses: possible cellular basis for white matter injury. Tohoku J Exp Med. 2014;234:299-307 pubmed
western blot; mouse; 1:1000	May V, Ettle B, Poehler A, Nuber S, Ubhi K, Rockenstein E, et al. ?-Synuclein impairs oligodendrocyte progenitor maturation in multiple system atrophy. Neurobiol Aging. 2014;35:2357-68 pubmed publisher
immunohistochemistry - frozen section; rhesus macaque; 10 ug/mL	Ramesh G, Santana Gould L, Inglis F, England J, Philipp M. The Lyme disease spirochete Borrelia burgdorferi induces inflammation and apoptosis in cells from dorsal root ganglia. J Neuroinflammation. 2013;10:88 pubmed publisher
	Feng T, Sheng R, Solé Domènech S, Ullah M, Zhou X, Mendoza C, et al. A role of the frontotemporal lobar degeneration risk factor TMEM106B in myelination. Brain. 2020;143:2255-2271 pubmed publisher
	Rabl R, Breitschaedel C, Flunkert S, Duller S, Amschl D, Neddens J, et al. Early start of progressive motor deficits in Line 61 ?-synuclein transgenic mice. BMC Neurosci. 2017;18:22 pubmed publisher
	Li X, Floriddia E, Toskas K, Fernandes K, Guérout N, Barnabe Heider F. Regenerative Potential of Ependymal Cells for Spinal Cord Injuries Over Time. EBioMedicine. 2016;13:55-65 pubmed publisher
	Baskin J, Wu X, Christiano R, Oh M, Schauder C, Gazzerro E, et al. The leukodystrophy protein FAM126A (hyccin) regulates PtdIns(4)P synthesis at the plasma membrane. Nat Cell Biol. 2016;18:132-8 pubmed publisher
	Kaneko M, Noguchi T, Ikegami S, Sakurai T, Kakita A, Toyoshima Y, et al. Zinc transporters ZnT3 and ZnT6 are downregulated in the spinal cords of patients with sporadic amyotrophic lateral sclerosis. J Neurosci Res. 2015;93:370-9 pubmed publisher
	Klein R, Blaschke S, Neumaier B, Endepols H, Graf R, Keuters M, et al. The synthetic NCAM mimetic peptide FGL mobilizes neural stem cells in vitro and in vivo. Stem Cell Rev. 2014;10:539-47 pubmed publisher
	Sevc J, Matiašová A, Kútna V, Daxnerova Z. Evidence that the central canal lining of the spinal cord contributes to oligodendrogenesis during postnatal development and adulthood in intact rats. J Comp Neurol. 2014;522:3194-207 pubmed publisher
	Reyes J, Rey N, Bousset L, Melki R, Brundin P, Angot E. Alpha-synuclein transfers from neurons to oligodendrocytes. Glia. 2014;62:387-98 pubmed publisher
	Verginelli F, Perin A, Dali R, Fung K, Lo R, Longatti P, et al. Transcription factors FOXG1 and Groucho/TLE promote glioblastoma growth. Nat Commun. 2013;4:2956 pubmed publisher
	Sydler T, Lesser M, Waldern N, Dennler M, Bode Lesniewska B, Pospischil A, et al. Malignant peripheral nerve sheath tumour in the nasopharynx of a cow. Schweiz Arch Tierheilkd. 2013;155:621-6 pubmed publisher
	Arun T, Tomassini V, Sbardella E, de Ruiter M, Matthews L, Leite M, et al. Targeting ASIC1 in primary progressive multiple sclerosis: evidence of neuroprotection with amiloride. Brain. 2013;136:106-15 pubmed publisher
	Zhan R, Yamamoto M, Ueki T, Yoshioka N, Tanaka K, Morisaki H, et al. A DEAD-box RNA helicase Ddx54 protein in oligodendrocytes is indispensable for myelination in the central nervous system. J Neurosci Res. 2013;91:335-48 pubmed publisher
	Sivukhina E, Helbling J, Minni A, Schäfer H, Pallet V, Jirikowski G, et al. Intrinsic expression of transcortin in neural cells of the mouse brain: a histochemical and molecular study. J Exp Biol. 2013;216:245-52 pubmed publisher
	Hou J, Riise J, Pakkenberg B. Application of immunohistochemistry in stereology for quantitative assessment of neural cell populations illustrated in the Göttingen minipig. PLoS ONE. 2012;7:e43556 pubmed publisher
	Claus H, Walberer M, Simard M, Emig B, Muesken S, Rueger M, et al. NG2 and NG2-positive cells delineate focal cerebral infarct demarcation in rats. Neuropathology. 2013;33:30-8 pubmed publisher
	Gazzerro E, Baldassari S, Giacomini C, Musante V, Fruscione F, La Padula V, et al. Hyccin, the molecule mutated in the leukodystrophy hypomyelination and congenital cataract (HCC), is a neuronal protein. PLoS ONE. 2012;7:e32180 pubmed publisher
	Hansmann F, Pringproa K, Ulrich R, Sun Y, Herder V, Kreutzer M, et al. Highly malignant behavior of a murine oligodendrocyte precursor cell line following transplantation into the demyelinated and nondemyelinated central nervous system. Cell Transplant. 2012;21:1161-75 pubmed publisher
	Okada H, Iwamaru Y, Fukuda S, Yokoyama T, Mohri S. Detection of disease-associated prion protein in the optic nerve and the adrenal gland of cattle with bovine spongiform encephalopathy by using highly sensitive immunolabeling procedures. J Histochem Cytochem. 2012;60:290-300 pubmed publisher
	Rossmeisl J, Piñeyro P, Sponenberg D, Garman R, Jortner B. Clinicopathologic features of intracranial central neurocytomas in 2 dogs. J Vet Intern Med. 2012;26:186-91 pubmed publisher
	Mühlebner A, Coras R, Kobow K, Feucht M, Czech T, Stefan H, et al. Neuropathologic measurements in focal cortical dysplasias: validation of the ILAE 2011 classification system and diagnostic implications for MRI. Acta Neuropathol. 2012;123:259-72 pubmed publisher
	De Biase L, Kang S, Baxi E, Fukaya M, Pucak M, Mishina M, et al. NMDA receptor signaling in oligodendrocyte progenitors is not required for oligodendrogenesis and myelination. J Neurosci. 2011;31:12650-62 pubmed publisher
	Deng H, Chen W, Hong S, Boycott K, Gorrie G, Siddique N, et al. Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia. Nature. 2011;477:211-5 pubmed publisher
	Maxwell M, Tomkinson E, Nobles J, Wizeman J, Amore A, Quinti L, et al. The Sirtuin 2 microtubule deacetylase is an abundant neuronal protein that accumulates in the aging CNS. Hum Mol Genet. 2011;20:3986-96 pubmed publisher
	Raasch J, Zeller N, Van Loo G, Merkler D, Mildner A, Erny D, et al. IkappaB kinase 2 determines oligodendrocyte loss by non-cell-autonomous activation of NF-kappaB in the central nervous system. Brain. 2011;134:1184-98 pubmed publisher
	Vergo S, Craner M, Etzensperger R, Attfield K, Friese M, Newcombe J, et al. Acid-sensing ion channel 1 is involved in both axonal injury and demyelination in multiple sclerosis and its animal model. Brain. 2011;134:571-84 pubmed publisher
	Zhang J, Giesert F, Kloos K, Vogt Weisenhorn D, Aigner L, Wurst W, et al. A powerful transgenic tool for fate mapping and functional analysis of newly generated neurons. BMC Neurosci. 2010;11:158 pubmed publisher
	Shen L, Li Y, Chopp M. Astrocytic endogenous glial cell derived neurotrophic factor production is enhanced by bone marrow stromal cell transplantation in the ischemic boundary zone after stroke in adult rats. Glia. 2010;58:1074-81 pubmed publisher
	Liu Y, Bando Y, Vargas Lowy D, Elyaman W, Khoury S, Huang T, et al. CD200R1 agonist attenuates mechanisms of chronic disease in a murine model of multiple sclerosis. J Neurosci. 2010;30:2025-38 pubmed publisher
	Muller D, Greenland K, Speth R, Middendorff R. Neuronal differentiation of NG108-15 cells has impact on nitric oxide- and membrane (natriuretic peptide receptor-A) cyclic GMP-generating proteins. Mol Cell Endocrinol. 2010;320:118-27 pubmed publisher
	Kuleshova L, Tan F, Magalhaes R, Gouk S, Lee K, Dawe G. Effective cryopreservation of neural stem or progenitor cells without serum or proteins by vitrification. Cell Transplant. 2009;18:135-44 pubmed
	Harrer M, von Büdingen H, Stoppini L, Alliod C, Pouly S, Fischer K, et al. Live imaging of remyelination after antibody-mediated demyelination in an ex-vivo model for immune mediated CNS damage. Exp Neurol. 2009;216:431-8 pubmed
	Zhang J, Brodie C, Li Y, Zheng X, Roberts C, Lu M, et al. Bone marrow stromal cell therapy reduces proNGF and p75 expression in mice with experimental autoimmune encephalomyelitis. J Neurol Sci. 2009;279:30-8 pubmed publisher
	Mann S, Versmold B, Marx R, Stahlhofen S, Dietzel I, Heumann R, et al. Corticosteroids reverse cytokine-induced block of survival and differentiation of oligodendrocyte progenitor cells from rats. J Neuroinflammation. 2008;5:39 pubmed publisher
	Tracy E, Aldrink J, Panosian J, Beam D, Thacker J, Reese M, et al. Isolation of oligodendrocyte-like cells from human umbilical cord blood. Cytotherapy. 2008;10:518-25 pubmed publisher
	Chitnis T, Imitola J, Wang Y, Elyaman W, Chawla P, Sharuk M, et al. Elevated neuronal expression of CD200 protects Wlds mice from inflammation-mediated neurodegeneration. Am J Pathol. 2007;170:1695-712 pubmed
	Lyck L, Jelsing J, Jensen P, Lambertsen K, Pakkenberg B, Finsen B. Immunohistochemical visualization of neurons and specific glial cells for stereological application in the porcine neocortex. J Neurosci Methods. 2006;152:229-42 pubmed
	Dracheva S, Davis K, Chin B, Woo D, Schmeidler J, Haroutunian V. Myelin-associated mRNA and protein expression deficits in the anterior cingulate cortex and hippocampus in elderly schizophrenia patients. Neurobiol Dis. 2006;21:531-40 pubmed
	Tricaud N, Perrin Tricaud C, BRUSES J, Rutishauser U. Adherens junctions in myelinating Schwann cells stabilize Schmidt-Lanterman incisures via recruitment of p120 catenin to E-cadherin. J Neurosci. 2005;25:3259-69 pubmed

product information

Catalog Number :

MAB326R

Subcategory :

Neuroscience

Product Name :

Anti-CNPase Antibody, clone 11-5B

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P09543

Host Name :

Mouse

Antigen :

CNPase

Clone :

11-5B

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-CNPase Antibody, clone 11-5B

Cross Reactivity :

Bovine;Canine;Human;Mouse;Pig;Rat;Rabbit;Sheep

Background :

The enzyme 2', 3'-cyclic nucleotide 3'-phosphodi-esterase (CNP) is expressed at high levels by oligodendrocytes in the central nervous system and by Schwann cells in the peripheral nervous system (Sprinkle, 1989). By virtue of this cell-specific expression, CNP is recognized as a characteristic marker for these two myelin-producing glial cell types (Sprinkle, 1989; Kim et al., 1984; Sheedlo & Sprinkle, 1984; McMorris et al., 1984). Beyond its enzymatic activity of cleaving the 2', 3'-cyclic terminus of nucleotides (Sprinkle, 1989), the physiological role of CNP is still under investigation. CNP activity has been correlated with myelin and myelin formation, and a dramatic decrease in CNP activity is associated with demyelinating diseases such as multiple sclerosis (Sprinkle, 1989). This enzyme is composed of two proteins, CNP1 (46 kD) and CNP2 (48 kD) (Sprinkle, 1989; Sprinkle et al., 1987). Although the ratio of CNP1/CNP2 may vary from species to species, their shared primary sequence is conserved phyIogenetically. CNP has recently been localized to human chromosome 17 by amplification of somatic cell hybrid DNA using the polymerase chain reaction (PCR) and by Southern blotting of Hind Ill genomic DNA digests (Sprinkle et al., 1991). Since anti-CNP reacts with a highly conserved region of the enzyme, it can be considered as pan-anti-CNP (Sprinkle et al., 1987). Anti-CNP can be used as a marker to identify Schwann cells and oligodendrocytes in cell cuIture and in tissue sections, as well as to localize CNP in cell membrane fractions. As CNP is expressed relatively early in postnatal development, anti-CNP is especially useful for the early identification of oligodendrocytes.

Immunogen :

Purified human 2’, 3’-cyclic nucleotide 3’-phosphodiesterase

Specificity :

Anti-CNP reacts with both CNP1 and CNP2 in many species.

Package Size :

100 μg

Uses :

Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Stable for 1 year at 2-8ºC from date of receipt.