CNPase Antibody, clone 11-5B, Alexa Fluor®488 Conjugate | EMD Millipore MAB326A4 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

CNPase Antibody, clone 11-5B, Alexa Fluor®488 Conjugate

catalog :

MAB326A4

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

AF488

clone name :

11-5B

The same clone is also sold as:

LifeSpan Biosciences: LS-C88110
Abcam: ab6319
MilliporeSigma: C5922

reactivity :

human, mouse, rat, dogs, bovine

application :

immunocytochemistry, immunohistochemistry - free floating section

citations: 23

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; rat; 1:200	Reyes J, Rey N, Bousset L, Melki R, Brundin P, Angot E. Alpha-synuclein transfers from neurons to oligodendrocytes. Glia. 2014;62:387-98 pubmed publisher
	Kaneko M, Noguchi T, Ikegami S, Sakurai T, Kakita A, Toyoshima Y, et al. Zinc transporters ZnT3 and ZnT6 are downregulated in the spinal cords of patients with sporadic amyotrophic lateral sclerosis. J Neurosci Res. 2015;93:370-9 pubmed publisher
	Klein R, Blaschke S, Neumaier B, Endepols H, Graf R, Keuters M, et al. The synthetic NCAM mimetic peptide FGL mobilizes neural stem cells in vitro and in vivo. Stem Cell Rev. 2014;10:539-47 pubmed publisher
	May V, Ettle B, Poehler A, Nuber S, Ubhi K, Rockenstein E, et al. ?-Synuclein impairs oligodendrocyte progenitor maturation in multiple system atrophy. Neurobiol Aging. 2014;35:2357-68 pubmed publisher
	Sevc J, Matiašová A, Kútna V, Daxnerova Z. Evidence that the central canal lining of the spinal cord contributes to oligodendrogenesis during postnatal development and adulthood in intact rats. J Comp Neurol. 2014;522:3194-207 pubmed publisher
	Sydler T, Lesser M, Waldern N, Dennler M, Bode Lesniewska B, Pospischil A, et al. Malignant peripheral nerve sheath tumour in the nasopharynx of a cow. Schweiz Arch Tierheilkd. 2013;155:621-6 pubmed publisher
	Ramesh G, Santana Gould L, Inglis F, England J, Philipp M. The Lyme disease spirochete Borrelia burgdorferi induces inflammation and apoptosis in cells from dorsal root ganglia. J Neuroinflammation. 2013;10:88 pubmed publisher
	Zhan R, Yamamoto M, Ueki T, Yoshioka N, Tanaka K, Morisaki H, et al. A DEAD-box RNA helicase Ddx54 protein in oligodendrocytes is indispensable for myelination in the central nervous system. J Neurosci Res. 2013;91:335-48 pubmed publisher
	Sivukhina E, Helbling J, Minni A, Schäfer H, Pallet V, Jirikowski G, et al. Intrinsic expression of transcortin in neural cells of the mouse brain: a histochemical and molecular study. J Exp Biol. 2013;216:245-52 pubmed publisher
	Claus H, Walberer M, Simard M, Emig B, Muesken S, Rueger M, et al. NG2 and NG2-positive cells delineate focal cerebral infarct demarcation in rats. Neuropathology. 2013;33:30-8 pubmed publisher
	Gazzerro E, Baldassari S, Giacomini C, Musante V, Fruscione F, La Padula V, et al. Hyccin, the molecule mutated in the leukodystrophy hypomyelination and congenital cataract (HCC), is a neuronal protein. PLoS ONE. 2012;7:e32180 pubmed publisher
	Hansmann F, Pringproa K, Ulrich R, Sun Y, Herder V, Kreutzer M, et al. Highly malignant behavior of a murine oligodendrocyte precursor cell line following transplantation into the demyelinated and nondemyelinated central nervous system. Cell Transplant. 2012;21:1161-75 pubmed publisher
	Okada H, Iwamaru Y, Fukuda S, Yokoyama T, Mohri S. Detection of disease-associated prion protein in the optic nerve and the adrenal gland of cattle with bovine spongiform encephalopathy by using highly sensitive immunolabeling procedures. J Histochem Cytochem. 2012;60:290-300 pubmed publisher
	Rossmeisl J, Piñeyro P, Sponenberg D, Garman R, Jortner B. Clinicopathologic features of intracranial central neurocytomas in 2 dogs. J Vet Intern Med. 2012;26:186-91 pubmed publisher
	Mühlebner A, Coras R, Kobow K, Feucht M, Czech T, Stefan H, et al. Neuropathologic measurements in focal cortical dysplasias: validation of the ILAE 2011 classification system and diagnostic implications for MRI. Acta Neuropathol. 2012;123:259-72 pubmed publisher
	De Biase L, Kang S, Baxi E, Fukaya M, Pucak M, Mishina M, et al. NMDA receptor signaling in oligodendrocyte progenitors is not required for oligodendrogenesis and myelination. J Neurosci. 2011;31:12650-62 pubmed publisher
	Maxwell M, Tomkinson E, Nobles J, Wizeman J, Amore A, Quinti L, et al. The Sirtuin 2 microtubule deacetylase is an abundant neuronal protein that accumulates in the aging CNS. Hum Mol Genet. 2011;20:3986-96 pubmed publisher
	Raasch J, Zeller N, Van Loo G, Merkler D, Mildner A, Erny D, et al. IkappaB kinase 2 determines oligodendrocyte loss by non-cell-autonomous activation of NF-kappaB in the central nervous system. Brain. 2011;134:1184-98 pubmed publisher
	Liu Y, Bando Y, Vargas Lowy D, Elyaman W, Khoury S, Huang T, et al. CD200R1 agonist attenuates mechanisms of chronic disease in a murine model of multiple sclerosis. J Neurosci. 2010;30:2025-38 pubmed publisher
	Harrer M, von Büdingen H, Stoppini L, Alliod C, Pouly S, Fischer K, et al. Live imaging of remyelination after antibody-mediated demyelination in an ex-vivo model for immune mediated CNS damage. Exp Neurol. 2009;216:431-8 pubmed
	Tracy E, Aldrink J, Panosian J, Beam D, Thacker J, Reese M, et al. Isolation of oligodendrocyte-like cells from human umbilical cord blood. Cytotherapy. 2008;10:518-25 pubmed publisher
	Chitnis T, Imitola J, Wang Y, Elyaman W, Chawla P, Sharuk M, et al. Elevated neuronal expression of CD200 protects Wlds mice from inflammation-mediated neurodegeneration. Am J Pathol. 2007;170:1695-712 pubmed
	Tricaud N, Perrin Tricaud C, BRUSES J, Rutishauser U. Adherens junctions in myelinating Schwann cells stabilize Schmidt-Lanterman incisures via recruitment of p120 catenin to E-cadherin. J Neurosci. 2005;25:3259-69 pubmed

product information

Catalog Number :

MAB326A4

Subcategory :

Neuroscience

Product Name :

Anti-CNPase Antibody, clone 11-5B, Alexa Fluor®488 Conjugate

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P09543

Host Name :

Mouse

Antigen :

CNPase

Clone :

11-5B

Conjugate :

AlexaFluor®488

Isotype :

IgG1

Product Description :

Anti-CNPase Antibody, clone 11-5B, Alexa Fluor®488 Conjugate

Cross Reactivity :

Rat;Human;Mouse;Rabbit;Pig;Canine;Bovine;Sheep

Background :

CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligodendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively. Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.

ALT Names :

2',3'-cyclic-nucleotide 3'-phosphodiesterase;CNP;CNPase

Immunogen :

Purified human brain CNPase

Specificity :

The unconjugated parent antibody (Cat. No. MAB326) reacts with CNPase, 48, and 46 kDa polypeptides by SDS-PAGE. Differentiates clearly oligodendrocytes and Schwann cells from neurons and astrocytes.

Package Size :

100 µL

Uses :

Immunocytochemistry

Storage :

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.