This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
CNPase Antibody, clone 11-5B
catalog :
MAB326
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
11-5B

The same clone is also sold as:
reactivity :
human, mouse, rat, dogs, bovine, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 72
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; human; 1:100; loading ...; fig s6a
Wheeler M, Jaronen M, Covacu R, Zandee S, Scalisi G, Rothhammer V, et al. Environmental Control of Astrocyte Pathogenic Activities in CNS Inflammation. Cell. 2019;176:581-596.e18 pubmed publisher
  • immunohistochemistry; human; fig 4g
Arrizabalaga O, Moreno Cugnon L, Auzmendi Iriarte J, Aldaz P, Ibanez de Caceres I, Garros Regulez L, et al. High expression of MKP1/DUSP1 counteracts glioma stem cell activity and mediates HDAC inhibitor response. Oncogenesis. 2017;6:401 pubmed publisher
  • western blot; mouse; loading ...; fig 1
Szewczyk L, Brozko N, Nagalski A, Röckle I, Werneburg S, Hildebrandt H, et al. ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons. Glia. 2017;65:34-49 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 4d
Anesten F, Holt M, Schéle E, Pálsdóttir V, Reimann F, Gribble F, et al. Preproglucagon neurons in the hindbrain have IL-6 receptor-α and show Ca2+ influx in response to IL-6. Am J Physiol Regul Integr Comp Physiol. 2016;311:R115-23 pubmed publisher
  • immunocytochemistry; rat; 1:200; fig 2
  • western blot; rat; 1:1000; fig 3
Chao C, Kan D, Lo T, Lu K, Chien C. Induction of neural differentiation in rat C6 glioma cells with taxol. Brain Behav. 2015;5:e00414 pubmed publisher
  • immunocytochemistry; mouse; 1:400; fig 3
Forni M, Ramos Maia Lobba A, Pereira Ferreira A, Sogayar M. Simultaneous Isolation of Three Different Stem Cell Populations from Murine Skin. PLoS ONE. 2015;10:e0140143 pubmed publisher
  • western blot; rat
Peckham H, Giuffrida L, Wood R, Gonsalvez D, Ferner A, Kilpatrick T, et al. Fyn is an intermediate kinase that BDNF utilizes to promote oligodendrocyte myelination. Glia. 2016;64:255-69 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 1
Bellesi M, de Vivo L, Tononi G, Cirelli C. Effects of sleep and wake on astrocytes: clues from molecular and ultrastructural studies. BMC Biol. 2015;13:66 pubmed publisher
  • immunocytochemistry; rat; 1:500; fig 5
Rabenstein M, Hucklenbroich J, Willuweit A, Ladwig A, Fink G, Schroeter M, et al. Osteopontin mediates survival, proliferation and migration of neural stem cells through the chemokine receptor CXCR4. Stem Cell Res Ther. 2015;6:99 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:100
  • immunocytochemistry; rat; 1:100
Yang L, Kan E, Lu J, Wu C, Ling E. Expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and its roles in activated microglia in vivo and in vitro. J Neuroinflammation. 2014;11:148 pubmed publisher
  • western blot; rat; 1:1000; fig 1
Ettle B, Reiprich S, Deusser J, Schlachetzki J, Xiang W, Prots I, et al. Intracellular alpha-synuclein affects early maturation of primary oligodendrocyte progenitor cells. Mol Cell Neurosci. 2014;62:68-78 pubmed publisher
  • western blot; mouse; 1:1000
May V, Ettle B, Poehler A, Nuber S, Ubhi K, Rockenstein E, et al. ?-Synuclein impairs oligodendrocyte progenitor maturation in multiple system atrophy. Neurobiol Aging. 2014;35:2357-68 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:400
Nakajima T, Yanagihara M, Nishii H. Temporal and regional patterns of Smad activation in the rat hippocampus following global ischemia. J Neurol Sci. 2014;337:25-37 pubmed publisher
  • immunohistochemistry; mouse; 1:200
Bellesi M, Pfister Genskow M, Maret S, Keles S, Tononi G, Cirelli C. Effects of sleep and wake on oligodendrocytes and their precursors. J Neurosci. 2013;33:14288-300 pubmed publisher
  • immunohistochemistry - frozen section; rhesus macaque; 10 ug/mL
Ramesh G, Santana Gould L, Inglis F, England J, Philipp M. The Lyme disease spirochete Borrelia burgdorferi induces inflammation and apoptosis in cells from dorsal root ganglia. J Neuroinflammation. 2013;10:88 pubmed publisher
  • immunohistochemistry; mouse
Maurin H, Seymour C, Lechat B, Borghgraef P, Devijver H, Jaworski T, et al. Tauopathy differentially affects cell adhesion molecules in mouse brain: early down-regulation of nectin-3 in stratum lacunosum moleculare. PLoS ONE. 2013;8:e63589 pubmed publisher
  • immunocytochemistry; mouse; 1:100
Ohtsuka N, Badurek S, Busslinger M, Benes F, Minichiello L, Rudolph U. GABAergic neurons regulate lateral ventricular development via transcription factor Pax5. Genesis. 2013;51:234-45 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:150
Jyothi V, Li M, Kilpatrick L, Smythe N, LaRue A, Zhou D, et al. Unmyelinated auditory type I spiral ganglion neurons in congenic Ly5.1 mice. J Comp Neurol. 2010;518:3254-71 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1,000
Ahlemeyer B, Neubert I, Kovacs W, Baumgart Vogt E. Differential expression of peroxisomal matrix and membrane proteins during postnatal development of mouse brain. J Comp Neurol. 2007;505:1-17 pubmed
Suo N, Guo Y, He B, Gu H, Xie X. Inhibition of MAPK/ERK pathway promotes oligodendrocytes generation and recovery of demyelinating diseases. Glia. 2019;67:1320-1332 pubmed publisher
Sun L, Mulinyawe S, Collins H, Ibrahim A, Li Q, Simon D, et al. Spatiotemporal Control of CNS Myelination by Oligodendrocyte Programmed Cell Death through the TFEB-PUMA Axis. Cell. 2018;175:1811-1826.e21 pubmed publisher
Guo Y, Suo N, Cui X, Yuan Q, Xie X. Vitamin C promotes oligodendrocytes generation and remyelination. Glia. 2018;66:1302-1316 pubmed publisher
Yoon H, Radulovic M, Walters G, Paulsen A, Drucker K, Starski P, et al. Protease activated receptor 2 controls myelin development, resiliency and repair. Glia. 2017;65:2070-2086 pubmed publisher
Rabl R, Breitschaedel C, Flunkert S, Duller S, Amschl D, Neddens J, et al. Early start of progressive motor deficits in Line 61 ?-synuclein transgenic mice. BMC Neurosci. 2017;18:22 pubmed publisher
Okuda A, Kurokawa S, Takehashi M, Maeda A, Fukuda K, Kubo Y, et al. Poly(ADP-ribose) polymerase inhibitors activate the p53 signaling pathway in neural stem/progenitor cells. BMC Neurosci. 2017;18:14 pubmed publisher
Makar T, Gerzanich V, Nimmagadda V, Jain R, Lam K, Mubariz F, et al. Silencing of Abcc8 or inhibition of newly upregulated Sur1-Trpm4 reduce inflammation and disease progression in experimental autoimmune encephalomyelitis. J Neuroinflammation. 2015;12:210 pubmed publisher
Baskin J, Wu X, Christiano R, Oh M, Schauder C, Gazzerro E, et al. The leukodystrophy protein FAM126A (hyccin) regulates PtdIns(4)P synthesis at the plasma membrane. Nat Cell Biol. 2016;18:132-8 pubmed publisher
Kaneko M, Noguchi T, Ikegami S, Sakurai T, Kakita A, Toyoshima Y, et al. Zinc transporters ZnT3 and ZnT6 are downregulated in the spinal cords of patients with sporadic amyotrophic lateral sclerosis. J Neurosci Res. 2015;93:370-9 pubmed publisher
Klein R, Blaschke S, Neumaier B, Endepols H, Graf R, Keuters M, et al. The synthetic NCAM mimetic peptide FGL mobilizes neural stem cells in vitro and in vivo. Stem Cell Rev. 2014;10:539-47 pubmed publisher
Sevc J, Matiašová A, Kútna V, Daxnerova Z. Evidence that the central canal lining of the spinal cord contributes to oligodendrogenesis during postnatal development and adulthood in intact rats. J Comp Neurol. 2014;522:3194-207 pubmed publisher
Maurin H, Chong S, Kraev I, Davies H, Kremer A, Seymour C, et al. Early structural and functional defects in synapses and myelinated axons in stratum lacunosum moleculare in two preclinical models for tauopathy. PLoS ONE. 2014;9:e87605 pubmed publisher
Milesi G, Garbelli R, Zucca I, Aronica E, Spreafico R, Frassoni C. Assessment of human hippocampal developmental neuroanatomy by means of ex-vivo 7 T magnetic resonance imaging. Int J Dev Neurosci. 2014;34:33-41 pubmed publisher
Reyes J, Rey N, Bousset L, Melki R, Brundin P, Angot E. Alpha-synuclein transfers from neurons to oligodendrocytes. Glia. 2014;62:387-98 pubmed publisher
Sydler T, Lesser M, Waldern N, Dennler M, Bode Lesniewska B, Pospischil A, et al. Malignant peripheral nerve sheath tumour in the nasopharynx of a cow. Schweiz Arch Tierheilkd. 2013;155:621-6 pubmed publisher
Herder V, Hansmann F, Wohlsein P, Peters M, Varela M, Palmarini M, et al. Immunophenotyping of inflammatory cells associated with Schmallenberg virus infection of the central nervous system of ruminants. PLoS ONE. 2013;8:e62939 pubmed publisher
Zhan R, Yamamoto M, Ueki T, Yoshioka N, Tanaka K, Morisaki H, et al. A DEAD-box RNA helicase Ddx54 protein in oligodendrocytes is indispensable for myelination in the central nervous system. J Neurosci Res. 2013;91:335-48 pubmed publisher
Sivukhina E, Helbling J, Minni A, Schäfer H, Pallet V, Jirikowski G, et al. Intrinsic expression of transcortin in neural cells of the mouse brain: a histochemical and molecular study. J Exp Biol. 2013;216:245-52 pubmed publisher
Xiao J, Ferner A, Wong A, Denham M, Kilpatrick T, Murray S. Extracellular signal-regulated kinase 1/2 signaling promotes oligodendrocyte myelination in vitro. J Neurochem. 2012;122:1167-80 pubmed publisher
Claus H, Walberer M, Simard M, Emig B, Muesken S, Rueger M, et al. NG2 and NG2-positive cells delineate focal cerebral infarct demarcation in rats. Neuropathology. 2013;33:30-8 pubmed publisher
Gazzerro E, Baldassari S, Giacomini C, Musante V, Fruscione F, La Padula V, et al. Hyccin, the molecule mutated in the leukodystrophy hypomyelination and congenital cataract (HCC), is a neuronal protein. PLoS ONE. 2012;7:e32180 pubmed publisher
Hansmann F, Pringproa K, Ulrich R, Sun Y, Herder V, Kreutzer M, et al. Highly malignant behavior of a murine oligodendrocyte precursor cell line following transplantation into the demyelinated and nondemyelinated central nervous system. Cell Transplant. 2012;21:1161-75 pubmed publisher
Okada H, Iwamaru Y, Fukuda S, Yokoyama T, Mohri S. Detection of disease-associated prion protein in the optic nerve and the adrenal gland of cattle with bovine spongiform encephalopathy by using highly sensitive immunolabeling procedures. J Histochem Cytochem. 2012;60:290-300 pubmed publisher
Rossmeisl J, Piñeyro P, Sponenberg D, Garman R, Jortner B. Clinicopathologic features of intracranial central neurocytomas in 2 dogs. J Vet Intern Med. 2012;26:186-91 pubmed publisher
Mühlebner A, Coras R, Kobow K, Feucht M, Czech T, Stefan H, et al. Neuropathologic measurements in focal cortical dysplasias: validation of the ILAE 2011 classification system and diagnostic implications for MRI. Acta Neuropathol. 2012;123:259-72 pubmed publisher
Sheridan G, Dev K. S1P1 receptor subtype inhibits demyelination and regulates chemokine release in cerebellar slice cultures. Glia. 2012;60:382-92 pubmed publisher
Zabierowski S, Baubet V, Himes B, Li L, Fukunaga Kalabis M, Patel S, et al. Direct reprogramming of melanocytes to neural crest stem-like cells by one defined factor. Stem Cells. 2011;29:1752-62 pubmed publisher
De Biase L, Kang S, Baxi E, Fukaya M, Pucak M, Mishina M, et al. NMDA receptor signaling in oligodendrocyte progenitors is not required for oligodendrogenesis and myelination. J Neurosci. 2011;31:12650-62 pubmed publisher
Maxwell M, Tomkinson E, Nobles J, Wizeman J, Amore A, Quinti L, et al. The Sirtuin 2 microtubule deacetylase is an abundant neuronal protein that accumulates in the aging CNS. Hum Mol Genet. 2011;20:3986-96 pubmed publisher
Shu Y, Yang Y, Qiu W, Lu Z, Li Y, Bao J, et al. Neuroprotection by ulinastatin in experimental autoimmune encephalomyelitis. Neurochem Res. 2011;36:1969-77 pubmed publisher
Sato J, Sasaki S, Yamada N, Tsuchitani M. Hereditary cerebellar degenerative disease (cerebellar cortical abiotrophy) in rabbits. Vet Pathol. 2012;49:621-8 pubmed publisher
Wang D, Lu Q, Shao B, Cui G, Wang Y, Liu Y, et al. An upregulation of SIAH1 after spinal cord injury in adult rats. J Mol Neurosci. 2011;45:134-44 pubmed publisher
Raasch J, Zeller N, Van Loo G, Merkler D, Mildner A, Erny D, et al. IkappaB kinase 2 determines oligodendrocyte loss by non-cell-autonomous activation of NF-kappaB in the central nervous system. Brain. 2011;134:1184-98 pubmed publisher
Xiao J, Wong A, Willingham M, van den Buuse M, Kilpatrick T, Murray S. Brain-derived neurotrophic factor promotes central nervous system myelination via a direct effect upon oligodendrocytes. Neurosignals. 2010;18:186-202 pubmed publisher
Yao S, Ljunggren Rose A, Chandramohan N, Whetsell W, Sriram S. In vitro and in vivo induction and activation of nNOS by LPS in oligodendrocytes. J Neuroimmunol. 2010;229:146-56 pubmed publisher
Androutsellis Theotokis A, Walbridge S, Park D, Lonser R, McKay R. Cholera toxin regulates a signaling pathway critical for the expansion of neural stem cell cultures from the fetal and adult rodent brains. PLoS ONE. 2010;5:e10841 pubmed publisher
Androutsellis Theotokis A, Rueger M, Park D, Boyd J, Padmanabhan R, Campanati L, et al. Angiogenic factors stimulate growth of adult neural stem cells. PLoS ONE. 2010;5:e9414 pubmed publisher
Liu Y, Bando Y, Vargas Lowy D, Elyaman W, Khoury S, Huang T, et al. CD200R1 agonist attenuates mechanisms of chronic disease in a murine model of multiple sclerosis. J Neurosci. 2010;30:2025-38 pubmed publisher
Rishal I, Michaelevski I, Rozenbaum M, Shinder V, Medzihradszky K, Burlingame A, et al. Axoplasm isolation from peripheral nerve. Dev Neurobiol. 2010;70:126-33 pubmed publisher
Harrer M, von Büdingen H, Stoppini L, Alliod C, Pouly S, Fischer K, et al. Live imaging of remyelination after antibody-mediated demyelination in an ex-vivo model for immune mediated CNS damage. Exp Neurol. 2009;216:431-8 pubmed
Liu J, Zang D. Response of neural precursor cells in the brain of Parkinson's disease mouse model after LIF administration. Neurol Res. 2009;31:681-6 pubmed publisher
Ahn S, Byun K, Kim D, Lee K, Yoo J, Kim S, et al. Olig2-induced neural stem cell differentiation involves downregulation of Wnt signaling and induction of Dickkopf-1 expression. PLoS ONE. 2008;3:e3917 pubmed publisher
Lindahl J, Kjellsen B, Tigert J, Miskimins R. In utero PCP exposure alters oligodendrocyte differentiation and myelination in developing rat frontal cortex. Brain Res. 2008;1234:137-47 pubmed publisher
Tracy E, Aldrink J, Panosian J, Beam D, Thacker J, Reese M, et al. Isolation of oligodendrocyte-like cells from human umbilical cord blood. Cytotherapy. 2008;10:518-25 pubmed publisher
Androutsellis Theotokis A, Murase S, Boyd J, Park D, Hoeppner D, Ravin R, et al. Generating neurons from stem cells. Methods Mol Biol. 2008;438:31-8 pubmed publisher
Pringproa K, Kumnok J, Ulrich R, Baumgartner W, Wewetzer K. In vitro characterization of a murine oligodendrocyte precursor cell line (BO-1) following spontaneous immortalization. Int J Dev Neurosci. 2008;26:283-91 pubmed publisher
Chitnis T, Imitola J, Wang Y, Elyaman W, Chawla P, Sharuk M, et al. Elevated neuronal expression of CD200 protects Wlds mice from inflammation-mediated neurodegeneration. Am J Pathol. 2007;170:1695-712 pubmed
Juan L, Dawei Z, Julie A. Increased number and differentiation of neural precursor cells in the brainstem of superoxide dismutase 1(G93A) (G1H) transgenic mouse model of amyotrophic lateral sclerosis. Neurol Res. 2007;29:204-9 pubmed
Dugas J, Tai Y, Speed T, Ngai J, Barres B. Functional genomic analysis of oligodendrocyte differentiation. J Neurosci. 2006;26:10967-83 pubmed
Li Q, Hosaka N, Cui W, Wang X, Cui Y, Cui Y, et al. Lin(-)CD34(-) bone marrow cells from adult mice can differentiate into neural-like cells. Neurosci Lett. 2006;408:51-6 pubmed
Hinman J, Peters A, Cabral H, Rosene D, Hollander W, Rasband M, et al. Age-related molecular reorganization at the node of Ranvier. J Comp Neurol. 2006;495:351-62 pubmed
Ahlemeyer B, Baumgart Vogt E. Optimized protocols for the simultaneous preparation of primary neuronal cultures of the neocortex, hippocampus and cerebellum from individual newborn (P0.5) C57Bl/6J mice. J Neurosci Methods. 2005;149:110-20 pubmed
Tricaud N, Perrin Tricaud C, BRUSES J, Rutishauser U. Adherens junctions in myelinating Schwann cells stabilize Schmidt-Lanterman incisures via recruitment of p120 catenin to E-cadherin. J Neurosci. 2005;25:3259-69 pubmed
product information
Catalog Number :
MAB326
Subcategory :
Neuroscience
Product Name :
Anti-CNPase Antibody, clone 11-5B
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P09543
Host Name :
Mouse
Antigen :
CNPase
Clone :
11-5B
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-CNPase Antibody, clone 11-5B
Cross Reactivity :
Bovine;Canine;Human;Mouse;Pig;Rat;Rabbit;Sheep
Background :
CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively. Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.
ALT Names :
2'3'-Cyclic Nucleotide 3'-Phosphohydrolase
Immunogen :
Purified human brain CNPase
Specificity :
CNPase, 48 and 46 kD polypeptides by SDS-PAGE. Differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc.
Package Size :
100 μg
Uses :
Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting
Storage :
Stable for 1 year at 2-8ºC from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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