Cytokeratin 18 Antibody, clone RGE53 | EMD Millipore MAB3234 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Cytokeratin 18 Antibody, clone RGE53

catalog :

MAB3234

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RGE53

The same clone is also sold as:

Novus Biologicals: NBP1-97715
OriGene: AM02003SU-N, BM6005P
Invitrogen: MA1-06326
Santa Cruz Biotechnology: sc-32329
LifeSpan Biosciences: LS-C146731

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - free floating section

citations: 23

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:50; loading ...; fig 4b	Kam J, Dumontier E, Baim C, Brignall A, Mendes da Silva D, Cowan M, et al. RGMB and neogenin control cell differentiation in the developing olfactory epithelium. Development. 2016;143:1534-46 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:200; fig 4	Ostrowski S, Wright M, Bolock A, Geng X, Maricich S. Ectopic Atoh1 expression drives Merkel cell production in embryonic, postnatal and adult mouse epidermis. Development. 2015;142:2533-44 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100	Petrakova O, Terskikh V, Chernioglo E, Ashapkin V, Bragin E, Shtratnikova V, et al. Comparative analysis reveals similarities between cultured submandibular salivary gland cells and liver progenitor cells. Springerplus. 2014;3:183 pubmed publisher
immunocytochemistry; human; 1:100	Muchkaeva I, Dashinimaev E, Artyuhov A, Myagkova E, Vorotelyak E, Yegorov Y, et al. Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells. Acta Naturae. 2014;6:45-53 pubmed
	Ollech D, Pflästerer T, Shellard A, Zambarda C, Spatz J, Marcq P, et al. An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells. Nat Commun. 2020;11:472 pubmed publisher
	Li J, Said A, Ge X, Wang W, Zhang Y, Jin T. Development and validation of immortalized bovine mammary epithelial cell line as an in vitro model for the study of mammary gland functions. Cytotechnology. 2018;70:67-82 pubmed publisher
	Tadeu A, Lin S, Hou L, Chung L, Zhong M, Zhao H, et al. Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells. PLoS ONE. 2015;10:e0122493 pubmed publisher
	Tadeu A, Horsley V. Notch signaling represses p63 expression in the developing surface ectoderm. Development. 2013;140:3777-86 pubmed publisher
	Sun W, Tiemessen D, Sloff M, Lammers R, de Mulder E, Hilborn J, et al. Improving the cell distribution in collagen-coated poly-caprolactone knittings. Tissue Eng Part C Methods. 2012;18:731-9 pubmed
	Pal R, Mamidi M, Das A, Bhonde R. Diverse effects of dimethyl sulfoxide (DMSO) on the differentiation potential of human embryonic stem cells. Arch Toxicol. 2012;86:651-61 pubmed publisher
	Yuan X, Liu L, Fu Q, Wang C. Effects of ligustrazine on ureteral obstruction-induced renal tubulointerstitial fibrosis. Phytother Res. 2012;26:697-703 pubmed publisher
	Gokoffski K, Wu H, Beites C, Kim J, Kim E, Matzuk M, et al. Activin and GDF11 collaborate in feedback control of neuroepithelial stem cell proliferation and fate. Development. 2011;138:4131-42 pubmed publisher
	Essbauer S, Krautkrämer E, Herzog S, Pfeffer M. A new permanent cell line derived from the bank vole (Myodes glareolus) as cell culture model for zoonotic viruses. Virol J. 2011;8:339 pubmed publisher
	Hegg C, Jia C, Chick W, Restrepo D, Hansen A. Microvillous cells expressing IP3 receptor type 3 in the olfactory epithelium of mice. Eur J Neurosci. 2010;32:1632-45 pubmed publisher
	Schmack I, Berglin L, Nie X, Wen J, Kang S, Marcus A, et al. Modulation of choroidal neovascularization by subretinal injection of retinal pigment epithelium and polystyrene microbeads. Mol Vis. 2009;15:146-61 pubmed
	Shevchenko A, Demina V, Mazurok N, Zhelezova A, Efremov I, Shilov A, et al. [Extraembryonic endoderm stem cell lines from common voles of the genus Microtus]. Genetika. 2008;44:1477-85 pubmed
	Whitehead R, Robinson P. Establishment of conditionally immortalized epithelial cell lines from the intestinal tissue of adult normal and transgenic mice. Am J Physiol Gastrointest Liver Physiol. 2009;296:G455-60 pubmed publisher
	Hansen A, Finger T. Is TrpM5 a reliable marker for chemosensory cells? Multiple types of microvillous cells in the main olfactory epithelium of mice. BMC Neurosci. 2008;9:115 pubmed publisher
	Medawar A, Virolle T, Rostagno P, de la Forest Divonne S, Gambaro K, Rouleau M, et al. DeltaNp63 is essential for epidermal commitment of embryonic stem cells. PLoS ONE. 2008;3:e3441 pubmed publisher
	Ishizaka S, Ouji Y, Yoshikawa M, Nakatani K. Derivation and characterization of hepatocytes from embryonic stem cells in vitro. Methods Mol Biol. 2006;330:387-99 pubmed
	Jiang X, Tang L, Cao X, Zhou H, Xia Q, Wu J, et al. Two-dimensional gel electrophoresis maps of the proteome and phosphoproteome of primitively cultured rat mesangial cells. Electrophoresis. 2005;26:4540-62 pubmed
	Gambaro K, Aberdam E, Virolle T, Aberdam D, Rouleau M. BMP-4 induces a Smad-dependent apoptotic cell death of mouse embryonic stem cell-derived neural precursors. Cell Death Differ. 2006;13:1075-87 pubmed
	Bonne S, Gilbert B, Hatzfeld M, Chen X, Green K, van Roy F. Defining desmosomal plakophilin-3 interactions. J Cell Biol. 2003;161:403-16 pubmed

product information

Catalog Number :

MAB3234

Subcategory :

Cell Structure

Product Name :

Anti-Cytokeratin 18 Antibody, clone RGE53

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P05783

Host Name :

Mouse

Antigen :

Cytokeratin 18

Clone :

RGE53

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Cytokeratin 18 Antibody, clone RGE53

Cross Reactivity :

Canine;Chicken;Human;Hamster;Mouse;Pig;Rat;Rabbit

Background :

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.

Immunogen :

Cytoskeletal preparation from HeLa cells

Specificity :

Specifically recognizes glandular epithelial cells from gastro-intestinal tract, respiratory tract and urogenetical tract. As well as endocrine and exocrine tissues and mesothelial cells. It is unreactive with stratified squamous epithelia (esophagus, epidermis, ectocervix). In transitional epithelium of the bladder RGE53 only reacts with the superficial (umbrella) cells. RGE53 can distinguish adenocarcinomas and mesotheliomas from squamous cell carcinoma and non-epithelial tumours. The antibody is directed against 45 kD cytokeratin protein (cytokeratin 18). Cross reactivity with; Keratin from most mammals, birds.

Package Size :

100 μg

Uses :

Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Western Blotting

Storage :

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.