BrdU Antibody, clone IIB5 | EMD Millipore MAB3222 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

BrdU Antibody, clone IIB5

catalog :

MAB3222

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

IIB5

application :

immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, In-Cell Western

citations: 10

Reference
Li L, Xu Z, Liu G, Xu C, Wang Z, Li X, et al. Expression of 1N3R-Tau isoform inhibits cell proliferation by inducing S phase arrest in N2a cells. PLoS ONE. 2015;10:e0119865 pubmed publisher
Akitake Y, Katsuragi S, Hosokawa M, Mishima K, Ikeda T, Miyazato M, et al. Moderate maternal food restriction in mice impairs physical growth, behavior, and neurodevelopment of offspring. Nutr Res. 2015;35:76-87 pubmed publisher
Zhang P, Wu C, Liu N, Niu L, Yan Z, Feng Y, et al. Protocadherin 11 x regulates differentiation and proliferation of neural stem cell in vitro and in vivo. J Mol Neurosci. 2014;54:199-210 pubmed publisher
Zhao S, Tian H, Ma L, Yuan Y, Yu C, Ma M. Activity-dependent modulation of odorant receptor gene expression in the mouse olfactory epithelium. PLoS ONE. 2013;8:e69862 pubmed publisher
Shang Y, Doan C, Arnold T, Lee S, Tang A, Reichardt L, et al. Transcriptional corepressors HIPK1 and HIPK2 control angiogenesis via TGF-?-TAK1-dependent mechanism. PLoS Biol. 2013;11:e1001527 pubmed publisher
Sakane F, Miyamoto Y. N-cadherin regulates the proliferation and differentiation of ventral midbrain dopaminergic progenitors. Dev Neurobiol. 2013;73:518-29 pubmed publisher
Morandi A, Barbetti V, Riverso M, Dello Sbarba P, Rovida E. The colony-stimulating factor-1 (CSF-1) receptor sustains ERK1/2 activation and proliferation in breast cancer cell lines. PLoS ONE. 2011;6:e27450 pubmed publisher
Glaw J, Skalak T, Peirce S. Inhibition of canonical Wnt signaling increases microvascular hemorrhaging and venular remodeling in adult rats. Microcirculation. 2010;17:348-57 pubmed publisher
Tang M, Villaescusa J, Luo S, Guitarte C, Lei S, Miyamoto Y, et al. Interactions of Wnt/beta-catenin signaling and sonic hedgehog regulate the neurogenesis of ventral midbrain dopamine neurons. J Neurosci. 2010;30:9280-91 pubmed publisher
Madhavan L, Daley B, Paumier K, Collier T. Transplantation of subventricular zone neural precursors induces an endogenous precursor cell response in a rat model of Parkinson's disease. J Comp Neurol. 2009;515:102-15 pubmed publisher

product information

Catalog Number :

MAB3222

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-BrdU Antibody, clone IIB5

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

BrdU

Clone :

IIB5

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-BrdU Antibody, clone IIB5

Cross Reactivity :

All

Background :

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

ALT Names :

BrdU

Immunogen :

A fusion of mouse myeloma cells with spleen cells from a mouse intraperitoneally immunized with BrdU conjugated to bovine serum albumin.

Specificity :

Reacts specifically with bromodeoxyuridine. It also recognizes BrdU when incorporated into nuclear DNA.

Package Size :

100 μg

Uses :

Flow Cytometry;Immunocytochemistry;Immunohistochemistry (Paraffin)

Storage :

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.