This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Calnexin Antibody, ER marker, clone C8.B6
catalog :
MAB3126
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
C8.B6

The same clone is also sold as:
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation
citations: 26
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:2000; fig 7
  • immunoprecipitation; human; fig 3
  • western blot; human; 1:2000; fig 6
Prior K, Wittig I, Leisegang M, Groenendyk J, Weissmann N, Michalak M, et al. The Endoplasmic Reticulum Chaperone Calnexin Is a NADPH Oxidase NOX4 Interacting Protein. J Biol Chem. 2016;291:7045-59 pubmed publisher
  • immunocytochemistry; human; 1:100-1:250; loading ...; fig 6a
Vais H, Wang M, Mallilankaraman K, Payne R, McKennan C, Lock J, et al. ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein. elife. 2020;9: pubmed publisher
  • western blot; human; fig 4c
Wang W, Wang J, Lin W, Kao C, Hung M, Teng Y, et al. Progerin in muscle leads to thermogenic and metabolic defects via impaired calcium homeostasis. Aging Cell. 2020;19:e13090 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig s4
Yang C, Lin S, Chiang C, Wu Y, H ng W, Chang C, et al. Loss of GPNMB Causes Autosomal-Recessive Amyloidosis Cutis Dyschromica in Humans. Am J Hum Genet. 2018;102:219-232 pubmed publisher
  • western blot; human; loading ...; fig 4e
Liebsch F, Aurousseau M, Bethge T, McGuire H, Scolari S, Herrmann A, et al. Full-length cellular ?-secretase has a trimeric subunit stoichiometry, and its sulfur-rich transmembrane interaction site modulates cytosolic copper compartmentalization. J Biol Chem. 2017;292:13258-13270 pubmed publisher
  • western blot; rat; 1:500; fig 6
Russell S, Puttick D, Sawyer A, Potter D, Mague S, Carlezon W, et al. Nucleus Accumbens AMPA Receptors Are Necessary for Morphine-Withdrawal-Induced Negative-Affective States in Rats. J Neurosci. 2016;36:5748-62 pubmed publisher
  • immunocytochemistry; mouse; fig 2
Kanemoto S, Kobayashi Y, Yamashita T, Miyamoto T, Cui M, Asada R, et al. Luman is involved in osteoclastogenesis through the regulation of DC-STAMP expression, stability and localization. J Cell Sci. 2015;128:4353-65 pubmed publisher
  • immunocytochemistry; human; fig 3
DiGiuseppe S, Keiffer T, Bienkowska Haba M, Luszczek W, Guion L, Muller M, et al. Topography of the Human Papillomavirus Minor Capsid Protein L2 during Vesicular Trafficking of Infectious Entry. J Virol. 2015;89:10442-52 pubmed publisher
  • western blot; human; 1:1000; fig 1
Bidaux G, Borowiec A, Gordienko D, Beck B, Shapovalov G, Lemonnier L, et al. Epidermal TRPM8 channel isoform controls the balance between keratinocyte proliferation and differentiation in a cold-dependent manner. Proc Natl Acad Sci U S A. 2015;112:E3345-54 pubmed publisher
  • western blot; human; 1:2000; fig s2
Warnier M, Roudbaraki M, Derouiche S, Delcourt P, Bokhobza A, Prevarskaya N, et al. CACNA2D2 promotes tumorigenesis by stimulating cell proliferation and angiogenesis. Oncogene. 2015;34:5383-94 pubmed publisher
  • immunocytochemistry; human
Matsuzaki H, Fujimoto T, Tanaka M, Shirasawa S. Tespa1 is a novel component of mitochondria-associated endoplasmic reticulum membranes and affects mitochondrial calcium flux. Biochem Biophys Res Commun. 2013;433:322-6 pubmed publisher
Zhao Y, Tan C, Krauchunas A, Scharf A, Dietrich N, Warnhoff K, et al. The zinc transporter ZIPT-7.1 regulates sperm activation in nematodes. PLoS Biol. 2018;16:e2005069 pubmed publisher
Bidaux G, Gordienko D, Shapovalov G, Farfariello V, Borowiec A, Iamshanova O, et al. 4TM-TRPM8 channels are new gatekeepers of the ER-mitochondria Ca2+ transfer. Biochim Biophys Acta Mol Cell Res. 2018;1865:981-994 pubmed publisher
O Neill S, Mathis M, Kovacic L, Zhang S, Reinhardt J, Scholz D, et al. Quantitative interaction analysis permits molecular insights into functional NOX4 NADPH oxidase heterodimer assembly. J Biol Chem. 2018;293:8750-8760 pubmed publisher
Barucker C, Harmeier A, Weiske J, Fauler B, Albring K, Prokop S, et al. Nuclear translocation uncovers the amyloid peptide A?42 as a regulator of gene transcription. J Biol Chem. 2014;289:20182-91 pubmed publisher
Zumhagen S, Veldkamp M, Stallmeyer B, Baartscheer A, Eckardt L, Paul M, et al. A heterozygous deletion mutation in the cardiac sodium channel gene SCN5A with loss- and gain-of-function characteristics manifests as isolated conduction disease, without signs of Brugada or long QT syndrome. PLoS ONE. 2013;8:e67963 pubmed publisher
Bartolomé A, Guillen C, Benito M. Autophagy plays a protective role in endoplasmic reticulum stress-mediated pancreatic β cell death. Autophagy. 2012;8:1757-68 pubmed publisher
Kaden D, Harmeier A, Weise C, Münter L, Althoff V, Rost B, et al. Novel APP/A? mutation K16N produces highly toxic heteromeric A? oligomers. EMBO Mol Med. 2012;4:647-59 pubmed publisher
Tada M, Itoh S, Ishii Watabe A, Suzuki T, Kawasaki N. Functional analysis of the Notch ligand Jagged1 missense mutant proteins underlying Alagille syndrome. FEBS J. 2012;279:2096-107 pubmed publisher
Fassler M, Li X, Kaether C. Polar transmembrane-based amino acids in presenilin 1 are involved in endoplasmic reticulum localization, Pen2 protein binding, and ?-secretase complex stabilization. J Biol Chem. 2011;286:38390-6 pubmed publisher
Zenner H, Yoshimura S, Barr F, Crump C. Analysis of Rab GTPase-activating proteins indicates that Rab1a/b and Rab43 are important for herpes simplex virus 1 secondary envelopment. J Virol. 2011;85:8012-21 pubmed publisher
Methner D, Mayfield R. Ethanol alters endosomal recycling of human dopamine transporters. J Biol Chem. 2010;285:10310-7 pubmed publisher
Casini S, Tan H, Demirayak I, Remme C, Amin A, Scicluna B, et al. Tubulin polymerization modifies cardiac sodium channel expression and gating. Cardiovasc Res. 2010;85:691-700 pubmed publisher
Rajcevic U, Petersen K, Knol J, Loos M, Bougnaud S, Klychnikov O, et al. iTRAQ-based proteomics profiling reveals increased metabolic activity and cellular cross-talk in angiogenic compared with invasive glioblastoma phenotype. Mol Cell Proteomics. 2009;8:2595-612 pubmed publisher
Sitaram A, Piccirillo R, Palmisano I, Harper D, Dell Angelica E, Schiaffino M, et al. Localization to mature melanosomes by virtue of cytoplasmic dileucine motifs is required for human OCA2 function. Mol Biol Cell. 2009;20:1464-77 pubmed publisher
McSharry B, Burgert H, Owen D, Stanton R, Prod homme V, Sester M, et al. Adenovirus E3/19K promotes evasion of NK cell recognition by intracellular sequestration of the NKG2D ligands major histocompatibility complex class I chain-related proteins A and B. J Virol. 2008;82:4585-94 pubmed publisher
product information
Catalog Number :
MAB3126
Subcategory :
Neuroscience
Product Name :
Anti-Calnexin Antibody, ER marker, clone C8.B6
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P27824
Host Name :
Mouse
Antigen :
Calnexin
Clone :
C8.B6
Conjugate :
Ascites
Isotype :
IgG1
Product Description :
Anti-Calnexin Antibody, ER marker, clone C8.B6
Cross Reactivity :
Human;Mouse
Background :
Calnexin is a Ca++ binding protein found in endoplasmic reticulum membranes that binds to specific n-linked oligosaccharides found on newly synthesized proteins. It may play role in protein folding or retention and degradation of misfolded proteins in the endoplasmic reticulum.
ALT Names :
IP90 Protein; Integral Membrane Protein IP90; Phosphoprotein pp90
Immunogen :
Recombinant human calnexin
Specificity :
Recognizes Calnexin.
Package Size :
100 μg
Uses :
Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western Blotting
Storage :
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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