This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
A2B5 Antibody, clone A2B5-105
catalog :
MAB312
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A2B5-105
reactivity :
human, mouse, rat, pigs
application :
immunohistochemistry, immunocytochemistry, flow cytometry
citations: 30
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; rat; 1:500; loading ...; fig s1o
Neumann B, Baror R, Zhao C, SEGEL M, Dietmann S, Rawji K, et al. Metformin Restores CNS Remyelination Capacity by Rejuvenating Aged Stem Cells. Cell Stem Cell. 2019;25:473-485.e8 pubmed publisher
  • immunocytochemistry; rat; 1:300; loading ...; tbl 1
Syed Y, Abdulla S, Kotter M. Studying the Effects of Semaphorins on Oligodendrocyte Lineage Cells. Methods Mol Biol. 2017;1493:363-378 pubmed
  • immunocytochemistry; rat; 1:100
Chen Y, Zhang J, Shen L, Qi Q, Cheng X, Zhong Z, et al. Schwann cells induce Proliferation and Migration of Oligodendrocyte Precursor Cells Through Secretion of PDGF-AA and FGF-2. J Mol Neurosci. 2015;56:999-1008 pubmed publisher
  • immunocytochemistry; mouse; 1:250; loading ...; tbl 1
Terzic D, Maxon J, Krevitt L, DiBartolomeo C, Goyal T, Low W, et al. Directed Differentiation of Oligodendrocyte Progenitor Cells From Mouse Induced Pluripotent Stem Cells. Cell Transplant. 2016;25:411-24 pubmed publisher
  • immunocytochemistry; human; 1:200; tbl 1
Gervois P, Struys T, Hilkens P, Bronckaers A, Ratajczak J, Politis C, et al. Neurogenic maturation of human dental pulp stem cells following neurosphere generation induces morphological and electrophysiological characteristics of functional neurons. Stem Cells Dev. 2015;24:296-311 pubmed publisher
  • immunohistochemistry; rat; 1:250
Davis H, Gonzalez M, Stancescu M, Love R, Hickman J, Lambert S. A phenotypic culture system for the molecular analysis of CNS myelination in the spinal cord. Biomaterials. 2014;35:8840-8845 pubmed publisher
  • immunocytochemistry; human; 1:500
Sareen D, Gowing G, Sahabian A, Staggenborg K, Paradis R, Avalos P, et al. Human induced pluripotent stem cells are a novel source of neural progenitor cells (iNPCs) that migrate and integrate in the rodent spinal cord. J Comp Neurol. 2014;522:2707-28 pubmed publisher
  • immunocytochemistry; pigs ; 1:200
Lim J, McCullen S, Piedrahita J, Loboa E, Olby N. Alternating current electric fields of varying frequencies: effects on proliferation and differentiation of porcine neural progenitor cells. Cell Reprogram. 2013;15:405-12 pubmed publisher
  • immunocytochemistry; human; 1:100
Burel Vandenbos F, Turchi L, Benchetrit M, Fontas E, Pedeutour Z, Rigau V, et al. Cells with intense EGFR staining and a high nuclear to cytoplasmic ratio are specific for infiltrative glioma: a useful marker in neuropathological practice. Neuro Oncol. 2013;15:1278-88 pubmed publisher
SEGEL M, Neumann B, Hill M, Weber I, Viscomi C, Zhao C, et al. Niche stiffness underlies the ageing of central nervous system progenitor cells. Nature. 2019;573:130-134 pubmed publisher
Baror R, Neumann B, SEGEL M, Chalut K, Fancy S, Schafer D, et al. Transforming growth factor-beta renders ageing microglia inhibitory to oligodendrocyte generation by CNS progenitors. Glia. 2019;67:1374-1384 pubmed publisher
Vega S, Liu E, Arvind V, Bushman J, Sung H, Becker M, et al. High-content image informatics of the structural nuclear protein NuMA parses trajectories for stem/progenitor cell lineages and oncogenic transformation. Exp Cell Res. 2017;351:11-23 pubmed publisher
Zhu B, Zhao C, Young F, Franklin R, Song B. Isolation and long-term expansion of functional, myelinating oligodendrocyte progenitor cells from neonatal rat brain. Curr Protoc Stem Cell Biol. 2014;31:2D.17.1-15 pubmed publisher
Kleinsimlinghaus K, Marx R, Serdar M, Bendix I, Dietzel I. Strategies for repair of white matter: influence of osmolarity and microglia on proliferation and apoptosis of oligodendrocyte precursor cells in different basal culture media. Front Cell Neurosci. 2013;7:277 pubmed publisher
Yoo M, Lee G, PARK C, Cohen R, Schachner M. Analysis of human embryonic stem cells with regulatable expression of the cell adhesion molecule l1 in regeneration after spinal cord injury. J Neurotrauma. 2014;31:553-64 pubmed publisher
Darbinyan A, Kaminski R, White M, Darbinian Sarkissian N, Khalili K. Polyomavirus JC infection inhibits differentiation of oligodendrocyte progenitor cells. J Neurosci Res. 2013;91:116-27 pubmed publisher
Ahlemeyer B, Kehr K, Richter E, Hirz M, Baumgart Vogt E, Herden C. Phenotype, differentiation, and function differ in rat and mouse neocortical astrocytes cultured under the same conditions. J Neurosci Methods. 2013;212:156-64 pubmed publisher
Barateiro A, Vaz A, Silva S, Fernandes A, Brites D. ER stress, mitochondrial dysfunction and calpain/JNK activation are involved in oligodendrocyte precursor cell death by unconjugated bilirubin. Neuromolecular Med. 2012;14:285-302 pubmed publisher
Ponce Regalado M, Ortuño Sahagún D, Zarate C, Gudiño Cabrera G. Ensheathing cell-conditioned medium directs the differentiation of human umbilical cord blood cells into aldynoglial phenotype cells. Hum Cell. 2012;25:51-60 pubmed publisher
Kuronen M, Hermansson M, Manninen O, Zech I, Talvitie M, Laitinen T, et al. Galactolipid deficiency in the early pathogenesis of neuronal ceroid lipofuscinosis model Cln8mnd : implications to delayed myelination and oligodendrocyte maturation. Neuropathol Appl Neurobiol. 2012;38:471-86 pubmed publisher
Ueki T, Tsuruo Y, Yamamoto Y, Yoshimura K, Takanaga H, Seiwa C, et al. A new monoclonal antibody, 4F2, specific for the oligodendroglial cell lineage, recognizes ATP-dependent RNA helicase Ddx54: possible association with myelin basic protein. J Neurosci Res. 2012;90:48-59 pubmed publisher
Murphy J, Archibald M, Baldridge W, Chauhan B. Endothelin-1-induced proliferation is reduced and Ca²? signaling is enhanced in endothelin B-deficient optic nerve head astrocytes. Invest Ophthalmol Vis Sci. 2011;52:7771-7 pubmed publisher
Lim J, Piedrahita J, Jackson L, Ghashghaei T, Olby N. Development of a model of sacrocaudal spinal cord injury in cloned Yucatan minipigs for cellular transplantation research. Cell Reprogram. 2010;12:689-97 pubmed publisher
Lim J, Boozer L, Mariani C, Piedrahita J, Olby N. Generation and characterization of neurospheres from canine adipose tissue-derived stromal cells. Cell Reprogram. 2010;12:417-25 pubmed publisher
Murphy J, Archibald M, Chauhan B. The role of endothelin-1 and its receptors in optic nerve head astrocyte proliferation. Br J Ophthalmol. 2010;94:1233-8 pubmed publisher
Mihaliak A, Gilbert C, Li L, Daou M, Moser R, Reeves A, et al. Clinically relevant doses of chemotherapy agents reversibly block formation of glioblastoma neurospheres. Cancer Lett. 2010;296:168-77 pubmed publisher
Chi G, Kim M, Kim D, Jiang M, Son Y. Schwann cells differentiated from spheroid-forming cells of rat subcutaneous fat tissue myelinate axons in the spinal cord injury. Exp Neurol. 2010;222:304-17 pubmed publisher
Markakis E, Sasaki M, Lankford K, Kocsis J. Convergence of cells from the progenitor fraction of adult olfactory bulb tissue to remyelinating glia in demyelinating spinal cord lesions. PLoS ONE. 2009;4:e7260 pubmed publisher
Kranich S, Hattermann K, Specht A, Lucius R, Mentlein R. VEGFR-3/Flt-4 mediates proliferation and chemotaxis in glial precursor cells. Neurochem Int. 2009;55:747-53 pubmed publisher
Zhao X, Jin W, Ju G. An in vitro study on the involvement of LINGO-1 and Rho GTPases in Nogo-A regulated differentiation of oligodendrocyte precursor cells. Mol Cell Neurosci. 2007;36:260-9 pubmed
product information
Catalog Number :
MAB312
Subcategory :
Neuroscience
Product Name :
Anti-A2B5 Antibody, clone A2B5-105
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Host Name :
Mouse
Antigen :
A2B5
Clone :
A2B5-105
Conjugate :
Purified
Isotype :
IgM
Product Description :
Anti-A2B5 Antibody, clone A2B5-105
Cross Reactivity :
Mammals
ALT Names :
Neuron Cell Surface Antigen
Immunogen :
Embryonic chicken retinal cells
Specificity :
Reacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia, and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Binds with GQ ganglioside on the plasma membrane of neurons of animals of all species tested to date (Eisenbarth, et al., 1979).
Package Size :
100 μg
Uses :
Flow Cytometry;Immunocytochemistry;Immunofluorescence;Immunohistochemistry
Storage :
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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