DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11 | EMD Millipore MAB3118 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11

catalog :

MAB3118

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2EL-1B11

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

citations: 4

Reference
D Arcy B, Swingle M, Schambeau L, Pannell L, Prakash A, Honkanen R. Development of a Synthetic 3-ketosteroid Δ1-dehydrogenase for the Generation of a Novel Catabolic Pathway Enabling Cholesterol Degradation in Human Cells. Sci Rep. 2019;9:5969 pubmed publisher
Ashford A, Dunkley T, Cockerill M, Rowlinson R, Baak L, Gallo R, et al. Identification of DYRK1B as a substrate of ERK1/2 and characterisation of the kinase activity of DYRK1B mutants from cancer and metabolic syndrome. Cell Mol Life Sci. 2016;73:883-900 pubmed publisher
Swedan S, Andrews J, Majumdar T, Musiyenko A, Barik S. Multiple functional domains and complexes of the two nonstructural proteins of human respiratory syncytial virus contribute to interferon suppression and cellular location. J Virol. 2011;85:10090-100 pubmed publisher
Swedan S, Musiyenko A, Barik S. Respiratory syncytial virus nonstructural proteins decrease levels of multiple members of the cellular interferon pathways. J Virol. 2009;83:9682-93 pubmed publisher

product information

Catalog Number :

MAB3118

Subcategory :

Epitope Tags & General Use

Product Name :

Anti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

DYKDDDDK (FLAG® epitope tag)

Clone :

2EL-1B11

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11

Cross Reactivity :

Vertebrates; E. coli

Background :

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins. FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Immunogen :

Synthetic peptide (Cys)-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys coupled to ovalbumin

Specificity :

DYKDDDDK sequence containing proteins. Reacts with both N-terminal and C-terminal fusion proteins.

Package Size :

100 µL

Uses :

ELISA;Immunocytochemistry;Immunofluorescence;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.