G Protein Goα Antibody, clone 2A | EMD Millipore MAB3073 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

G Protein Goα Antibody, clone 2A

catalog :

MAB3073

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2A

The same clone is also sold as:

LifeSpan Biosciences: LS-C152924

reactivity :

African green monkey, fall armyworm, human, mouse, rat, bovine, Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

citations: 36

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; human; 1:1000; loading ...; fig 2	Kamar S, Howlett M, Klooster J, Graaff W, Csikós T, Rabelink M, et al. Degenerated Cones in Cultured Human Retinas Can Successfully Be Optogenetically Reactivated. Int J Mol Sci. 2020;21: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 8a	Simmons A, Camerino M, Clemons M, Sukeena J, Bloomsburg S, Borghuis B, et al. Increased density and age-related sharing of synapses at the cone to OFF bipolar cell synapse in the mouse retina. J Comp Neurol. 2019;: pubmed publisher
immunohistochemistry; human; loading ...; fig 4	Hendrickson A, Zhang C. Development of cone photoreceptors and their synapses in the human and monkey fovea. J Comp Neurol. 2019;527:38-51 pubmed publisher
immunocytochemistry; mouse; 1:1000; fig 3	van Wyk M, Pielecka Fortuna J, LÃ¶wel S, Kleinlogel S. Restoring the ON Switch in Blind Retinas: Opto-mGluR6, a Next-Generation, Cell-Tailored Optogenetic Tool. PLoS Biol. 2015;13:e1002143 pubmed publisher
immunohistochemistry; mouse; 1:5000; fig 7F	PÃ©rez de Sevilla MÃ¼ller L, Sargoy A, FernÃ¡ndez SÃ¡nchez L, Rodriguez A, Liu J, Cuenca N, et al. Expression and cellular localization of the voltage-gated calcium channel Î±2Î´3 in the rodent retina. J Comp Neurol. 2015;523:1443-60 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:500	Tse D, Chung I, Wu S. Pharmacological inhibitions of glutamate transporters EAAT1 and EAAT2 compromise glutamate transport in photoreceptor to ON-bipolar cell synapses. Vision Res. 2014;103:49-62 pubmed publisher
western blot; fall armyworm; 0.3 ug/ml	Agosto M, Zhang Z, He F, Anastassov I, Wright S, MCGEHEE J, et al. Oligomeric state of purified transient receptor potential melastatin-1 (TRPM1), a protein essential for dim light vision. J Biol Chem. 2014;289:27019-33 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:500	Tse D, Chung I, Wu S. Possible roles of glutamate transporter EAAT5 in mouse cone depolarizing bipolar cell light responses. Vision Res. 2014;103:63-74 pubmed publisher
immunohistochemistry - free floating section; Xenopus laevis; 1:1000 western blot; Xenopus laevis; 1:10000	Nakada T, Hagino Yamagishi K, Nakanishi K, Yokosuka M, Saito T, Toyoda F, et al. Expression of G proteins in the olfactory receptor neurons of the newt Cynops pyrrhogaster: their unique projection into the olfactory bulbs. J Comp Neurol. 2014;522:3501-19 pubmed publisher
western blot; human	Devi S, Markandeya Y, Maddodi N, Dhingra A, Vardi N, Balijepalli R, et al. Metabotropic glutamate receptor 6 signaling enhances TRPM1 calcium channel function and increases melanin content in human melanocytes. Pigment Cell Melanoma Res. 2013;26:348-56 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:5,000 immunohistochemistry - paraffin section; rat; 1:5,000	de Sevilla Müller L, Liu J, Solomon A, Rodriguez A, Brecha N. Expression of voltage-gated calcium channel ?(2)?(4) subunits in the mouse and rat retina. J Comp Neurol. 2013;521:2486-501 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500	Kranz K, Dorgau B, Pottek M, Herrling R, Schultz K, Bolte P, et al. Expression of Pannexin1 in the outer plexiform layer of the mouse retina and physiological impact of its knockout. J Comp Neurol. 2013;521:1119-35 pubmed publisher
immunohistochemistry - frozen section; Xenopus laevis; 1:500	Wiechmann A, Sherry D. Melatonin receptors are anatomically organized to modulate transmission specifically to cone pathways in the retina of Xenopus laevis. J Comp Neurol. 2012;520:1115-27 pubmed publisher
immunohistochemistry - paraffin section; Xenopus laevis	Hagino Yamagishi K, Nakazawa H. Involvement of G?(olf)-expressing neurons in the vomeronasal system of Bufo japonicus. J Comp Neurol. 2011;519:3189-201 pubmed publisher
immunohistochemistry; African green monkey; fig 1	Dhingra A, Fina M, Neinstein A, Ramsey D, Xu Y, Fishman G, et al. Autoantibodies in melanoma-associated retinopathy target TRPM1 cation channels of retinal ON bipolar cells. J Neurosci. 2011;31:3962-7 pubmed publisher
immunohistochemistry - frozen section; African green monkey; 1:300	Sulaiman P, Fina M, Feddersen R, Vardi N. Ret-PCP2 colocalizes with protein kinase C in a subset of primate ON cone bipolar cells. J Comp Neurol. 2010;518:1098-112 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:250	Kawano J, Tanizawa Y, Shinoda K. Wolfram syndrome 1 (Wfs1) gene expression in the normal mouse visual system. J Comp Neurol. 2008;510:1-23 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500	O Brien B, Caldwell J, Ehring G, Bumsted O Brien K, Luo S, Levinson S. Tetrodotoxin-resistant voltage-gated sodium channels Na(v)1.8 and Na(v)1.9 are expressed in the retina. J Comp Neurol. 2008;508:940-51 pubmed publisher
immunohistochemistry; African green monkey; 1:500	Lee S, Grünert U. Connections of diffuse bipolar cells in primate retina are biased against S-cones. J Comp Neurol. 2007;502:126-40 pubmed
immunohistochemistry - frozen section; mouse	Gargini C, Terzibasi E, Mazzoni F, Strettoi E. Retinal organization in the retinal degeneration 10 (rd10) mutant mouse: a morphological and ERG study. J Comp Neurol. 2007;500:222-38 pubmed
	Cronin T, Vandenberghe L, Hantz P, Jüttner J, Reimann A, Kacsó A, et al. Efficient transduction and optogenetic stimulation of retinal bipolar cells by a synthetic adeno-associated virus capsid and promoter. EMBO Mol Med. 2014;6:1175-90 pubmed publisher
	de Andrade G, Long S, Fleming H, Li W, Fuerst P. DSCAM localization and function at the mouse cone synapse. J Comp Neurol. 2014;522:2609-33 pubmed publisher
	Vuong L, Brobst D, Ivanovic I, Sherry D, Al Ubaidi M. p53 selectively regulates developmental apoptosis of rod photoreceptors. PLoS ONE. 2013;8:e67381 pubmed publisher
	Hilgen G, Huebner A, Tanimoto N, Sothilingam V, Seide C, Garcia Garrido M, et al. Lack of the sodium-driven chloride bicarbonate exchanger NCBE impairs visual function in the mouse retina. PLoS ONE. 2012;7:e46155 pubmed publisher
	Tan P, Yu P, Balaratnasingam C, Cringle S, Morgan W, McAllister I, et al. Quantitative confocal imaging of the retinal microvasculature in the human retina. Invest Ophthalmol Vis Sci. 2012;53:5728-36 pubmed publisher
	Sherry D, Kanan Y, Hamilton R, Hoffhines A, Arbogast K, Fliesler S, et al. Differential developmental deficits in retinal function in the absence of either protein tyrosine sulfotransferase-1 or -2. PLoS ONE. 2012;7:e39702 pubmed publisher
	Nakamuta S, Nakamuta N, Taniguchi K. Distinct axonal projections from two types of olfactory receptor neurons in the middle chamber epithelium of Xenopus laevis. Cell Tissue Res. 2011;346:27-33 pubmed publisher
	Ray A, Lee E, Humayun M, Weiland J. Continuous electrical stimulation decreases retinal excitability but does not alter retinal morphology. J Neural Eng. 2011;8:045003 pubmed publisher
	Lassová L, Fina M, Sulaiman P, Vardi N. Immunocytochemical evidence that monkey rod bipolar cells use GABA. Eur J Neurosci. 2010;31:685-96 pubmed publisher
	Hernandez M, Rodriguez F, Sharma S, Vecino E. Immunohistochemical changes in rat retinas at various time periods of elevated intraocular pressure. Mol Vis. 2009;15:2696-709 pubmed
	Koike C, Obara T, Uriu Y, Numata T, Sanuki R, Miyata K, et al. TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade. Proc Natl Acad Sci U S A. 2010;107:332-7 pubmed publisher
	Clarke R, Hognason K, Brimacombe M, Townes Anderson E. Cone and rod cells have different target preferences in vitro as revealed by optical tweezers. Mol Vis. 2008;14:706-20 pubmed
	Raven M, Orton N, Nassar H, Williams G, Stell W, Jacobs G, et al. Early afferent signaling in the outer plexiform layer regulates development of horizontal cell morphology. J Comp Neurol. 2008;506:745-58 pubmed
	Beltran W, Wen R, Acland G, Aguirre G. Intravitreal injection of ciliary neurotrophic factor (CNTF) causes peripheral remodeling and does not prevent photoreceptor loss in canine RPGR mutant retina. Exp Eye Res. 2007;84:753-71 pubmed
	Sherry D, Mitchell R, Standifer K, du Plessis B. Distribution of plasma membrane-associated syntaxins 1 through 4 indicates distinct trafficking functions in the synaptic layers of the mouse retina. BMC Neurosci. 2006;7:54 pubmed publisher
	Brzezinski J, Brown N, Tanikawa A, Bush R, Sieving P, Vitaterna M, et al. Loss of circadian photoentrainment and abnormal retinal electrophysiology in Math5 mutant mice. Invest Ophthalmol Vis Sci. 2005;46:2540-51 pubmed

product information

Catalog Number :

MAB3073

Subcategory :

Cell Signaling

Product Name :

Anti-G Protein Goα Antibody, clone 2A

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P29777

Host Name :

Mouse

Antigen :

G Protein Goα

Clone :

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-G Protein Goα Antibody, clone 2A

Cross Reactivity :

Bovine;Guinea Pig;Human;Mouse;Rat

Immunogen :

Bovine brain Go-alpha purified by the method of Sternweis and Robishaw (J. Biol. Chem. [1984] 259, 13806).

Specificity :

Go-alpha. Strong reactivity with bovine (100%), human (70%), rat (61%) and mouse (54%). Slight reactivity with guinea pig (27%). Specificity confirmed by Western blot versus bovine G-alpha-t, rat Go-alpha, rat Gi-alpha-1, Gi-alpha-2, rat Gi-alpha-3, bovine Gs-alpha, and murine Go-alpha-A and Go-alpha-B. The carboxy-terminal splice variants of Go-alpha (Go-alpha-A and Go-alpha-B) expressed in bacterial lysates were compared for reactivity and found to be equal . As Go-alpha-A and Go-alpha-B have 95% homology to rat Go-alpha-1 and human Go-alpha-2, MAB3073 will detect these G-proteins also.

Package Size :

100 µg

Uses :

Western Blotting

Storage :

Maintain at 2-8°C in undiluted aliquots for upt to 6 months after date of receipt. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.