Connexin 43 Antibody, clone 4E6.2 | EMD Millipore MAB3067 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Connexin 43 Antibody, clone 4E6.2

catalog :

MAB3067

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4E6.2

The same clone is also sold as:

Abcam: ab79010

reactivity :

human, mouse, rat, pigs

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 32

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:200; loading ...; fig 3a	Gemel J, Su Z, Gileles Hillel A, Khalyfa A, Gozal D, Beyer E. Intermittent hypoxia causes NOX2-dependent remodeling of atrial connexins. BMC Cell Biol. 2017;18:7 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:50; fig 3	Zaglia T, Di Bona A, Chioato T, Basso C, Ausoni S, Mongillo M. Optimized protocol for immunostaining of experimental GFP-expressing and human hearts. Histochem Cell Biol. 2016;146:407-19 pubmed publisher
immunocytochemistry; human; 1:50; fig 2a	Siciliano C, Chimenti I, Bordin A, Ponti D, Iudicone P, Peruzzi M, et al. The potential of GMP-compliant platelet lysate to induce a permissive state for cardiovascular transdifferentiation in human mediastinal adipose tissue-derived mesenchymal stem cells. Biomed Res Int. 2015;2015:162439 pubmed publisher
western blot; mouse	Noritake K, Aki T, Funakoshi T, Unuma K, Uemura K. Direct Exposure to Ethanol Disrupts Junctional Cell-Cell Contact and Hippo-YAP Signaling in HL-1 Murine Atrial Cardiomyocytes. PLoS ONE. 2015;10:e0136952 pubmed publisher
immunocytochemistry; human; 1:100; fig 2	Zatti S, Martewicz S, Serena E, Uno N, Giobbe G, Kazuki Y, et al. Complete restoration of multiple dystrophin isoforms in genetically corrected Duchenne muscular dystrophy patient-derived cardiomyocytes. Mol Ther Methods Clin Dev. 2014;1:1 pubmed publisher
western blot; human; 2 ug/ml	Losa D, Köhler T, Bellec J, Dudez T, Crespin S, Bacchetta M, et al. Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells is mediated by gap junctional communication in a JNK-dependent manner. J Immunol. 2014;192:4804-12 pubmed publisher
immunohistochemistry - paraffin section; pigs ; 1:50 western blot; pigs ; 1:2000	Santiquet N, Robert C, Richard F. The dynamics of connexin expression, degradation and localisation are regulated by gonadotropins during the early stages of in vitro maturation of swine oocytes. PLoS ONE. 2013;8:e68456 pubmed publisher
immunoprecipitation; mouse	Bao M, Kanter E, Huang R, Maxeiner S, Frank M, Zhang Y, et al. Residual Cx45 and its relationship to Cx43 in murine ventricular myocardium. Channels (Austin). 2011;5:489-99 pubmed publisher
	Parameswaran R, Koehler K, Rotenberg M, Burke M, Kim J, Jeong K, et al. Optical stimulation of cardiac cells with a polymer-supported silicon nanowire matrix. Proc Natl Acad Sci U S A. 2019;116:413-421 pubmed publisher
	Li K, Zhou H, Zhan L, Shi Z, Sun W, Liu D, et al. Hypoxic Preconditioning Maintains GLT-1 Against Transient Global Cerebral Ischemia Through Upregulating Cx43 and Inhibiting c-Src. Front Mol Neurosci. 2018;11:344 pubmed publisher
	Veeraraghavan R, Hoeker G, Alvarez Laviada A, Hoagland D, Wan X, King D, et al. The adhesion function of the sodium channel beta subunit (β1) contributes to cardiac action potential propagation. elife. 2018;7: pubmed publisher
	Veeraraghavan R, Gourdie R. Stochastic optical reconstruction microscopy-based relative localization analysis (STORM-RLA) for quantitative nanoscale assessment of spatial protein organization. Mol Biol Cell. 2016;27:3583-3590 pubmed
	Veeraraghavan R, Lin J, Hoeker G, Keener J, Gourdie R, Poelzing S. Sodium channels in the Cx43 gap junction perinexus may constitute a cardiac ephapse: an experimental and modeling study. Pflugers Arch. 2015;467:2093-105 pubmed publisher
	Moore K, Bryant Z, Ghatnekar G, Singh U, Gourdie R, Potts J. A synthetic connexin 43 mimetic peptide augments corneal wound healing. Exp Eye Res. 2013;115:178-88 pubmed publisher
	Andrysik Z, Prochazkova J, Kabátková M, Umannová L, Simeckova P, Kohoutek J, et al. Aryl hydrocarbon receptor-mediated disruption of contact inhibition is associated with connexin43 downregulation and inhibition of gap junctional intercellular communication. Arch Toxicol. 2013;87:491-503 pubmed publisher
	Rhett J, Ongstad E, Jourdan J, Gourdie R. Cx43 associates with Na(v)1.5 in the cardiomyocyte perinexus. J Membr Biol. 2012;245:411-22 pubmed publisher
	Ferguson T, Scherer S. Neuronal cadherin (NCAD) increases sensory neurite formation and outgrowth on astrocytes. Neurosci Lett. 2012;522:108-12 pubmed publisher
	Pinder J, Taylor Harris P, Bennett P, Carter E, Hayes N, King M, et al. Isoforms of protein 4.1 are differentially distributed in heart muscle cells: relation of 4.1R and 4.1G to components of the Ca2+ homeostasis system. Exp Cell Res. 2012;318:1467-79 pubmed publisher
	Garcia Pinto A, de Matos V, Rocha V, Moraes Teixeira J, Carvalho J. Low-Intensity physical activity beneficially alters the ultrastructural renal morphology of spontaneously hypertensive rats. Clinics (Sao Paulo). 2011;66:855-63 pubmed
	Wang Y, Wu Y, Liu M, Wang J, Ju G. Nogo-66 inhibits the dye-coupling of astrocytic gap junctions in vitro. Neurochem Res. 2011;36:1129-34 pubmed publisher
	Azarashvili T, Baburina Y, Grachev D, Krestinina O, Evtodienko Y, Stricker R, et al. Calcium-induced permeability transition in rat brain mitochondria is promoted by carbenoxolone through targeting connexin43. Am J Physiol Cell Physiol. 2011;300:C707-20 pubmed publisher
	Yoon J, Nicholson L, Feng S, Vis J, Green C. A novel method of organotypic brain slice culture using connexin-specific antisense oligodeoxynucleotides to improve neuronal survival. Brain Res. 2010;1353:194-203 pubmed publisher
	Eberwein P, Hiss S, Auw Haedrich C, Sundmacher R, Hauer K, Boehringer D, et al. Epithelial marker expression in Salzmann nodular degeneration shows characteristics of limbal transient amplifying cells and alludes to an involvement of the epithelium in its pathogenesis. Acta Ophthalmol. 2010;88:e184-9 pubmed publisher
	Niger C, Hebert C, Stains J. Interaction of connexin43 and protein kinase C-delta during FGF2 signaling. BMC Biochem. 2010;11:14 pubmed publisher
	Davis D, Zhang Y, Smith R, Cheng K, Terrovitis J, Malliaras K, et al. Validation of the cardiosphere method to culture cardiac progenitor cells from myocardial tissue. PLoS ONE. 2009;4:e7195 pubmed publisher
	Kim D, Lee E, Martens T, Kara R, Chaudhry H, Itescu S, et al. Engineered cardiac tissues for in vitro assessment of contractile function and repair mechanisms. Conf Proc IEEE Eng Med Biol Soc. 2006;1:849-52 pubmed
	Giardina S, Mikami M, Goubaeva F, Yang J. Connexin 43 confers resistance to hydrogen peroxide-mediated apoptosis. Biochem Biophys Res Commun. 2007;362:747-52 pubmed
	Goubaeva F, Mikami M, Giardina S, Ding B, Abe J, Yang J. Cardiac mitochondrial connexin 43 regulates apoptosis. Biochem Biophys Res Commun. 2007;352:97-103 pubmed
	Tansey E, Kwaku K, Hammer P, Cowan D, Federman M, Levitsky S, et al. Reduction and redistribution of gap and adherens junction proteins after ischemia and reperfusion. Ann Thorac Surg. 2006;82:1472-9 pubmed
	Cabo C, Yao J, Boyden P, Chen S, Hussain W, Duffy H, et al. Heterogeneous gap junction remodeling in reentrant circuits in the epicardial border zone of the healing canine infarct. Cardiovasc Res. 2006;72:241-9 pubmed
	Zahs K, Ceelen P. Gap junctional coupling and connexin immunoreactivity in rabbit retinal glia. Vis Neurosci. 2006;23:1-10 pubmed
	Hunter A, Barker R, Zhu C, Gourdie R. Zonula occludens-1 alters connexin43 gap junction size and organization by influencing channel accretion. Mol Biol Cell. 2005;16:5686-98 pubmed

product information

Catalog Number :

MAB3067

Subcategory :

Cell Structure

Product Name :

Anti-Connexin 43 Antibody, clone 4E6.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P17302

Host Name :

Mouse

Antigen :

Connexin 43

Clone :

4E6.2

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Connexin 43 Antibody, clone 4E6.2

Cross Reactivity :

Mouse;Human;Rat;Pig;Canine

Background :

Connexins are highly homologous proteins that assemble as hexamers (connexons) to form gap junctions, small channels which allow for the passage of ions and other small molecules up to 1,200 daltons between neighboring cells. Gap junctions play an important role in a variety of cellular processes including homeostasis, intercellular metabolic cooperation and synchronization, morphogenesis, cell differentiation, and growth control. The most broadly studied connexins are the 26, 32, 43 and 45 kDa isoforms. Connexin 43 is a 382 a.a. protein belonging to the alpha-type (Group II) subfamily of connexin proteins and possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. Connexin 43 has been detected in a variety of tissues including: atrial and ventricular myocytes, granulose cells, astrocytes, fibroblasts, leptomeningeal cells, brain, lung, smooth muscle, uterine muscle, kidney, lens, epithelium, cornea and testis. Connexin 43 also may have a role in communication between migrating cardiac neural crest cells.

ALT Names :

Gap junction 43 kDa heart protein;connexin 43;gap junction protein, alpha 1, 43kDa;gap junction protein, alpha 1, 43kDa (connexin 43);gap junction protein, alpha-like

Immunogen :

A synthetic peptide corresponding to positions 252-270 of the mouse connexin 43 sequence. This sequence is identical in rat and bovine.

Specificity :

Connexin-43. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate. Mouse connexin 43 sequence is identical in rat and bovine, and differs by one amino acid in pig. This sequence has no known homology with other connexins. Also effective for detection of Cx43 in mouse keratinocyte cells and on frozen sections of rat heart.

Package Size :

100 µg

Uses :

ELISA;Immunocytochemistry;Immunohistochemistry;Western Blotting

Storage :

Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt.