This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
DNA Antibody, single stranded, clone 16-19
catalog :
MAB3034
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
16-19
reactivity :
human, mouse
application :
immunocytochemistry, flow cytometry, other
citations: 35
Published Application/Species/Sample/DilutionReference
  • other; mouse; 1:200; loading ...; fig s8b
Sarek G, Kotsantis P, Ruis P, Van Ly D, Margalef P, Borel V, et al. CDK phosphorylation of TRF2 controls t-loop dynamics during the cell cycle. Nature. 2019;: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 5a
Utani K, Fu H, Jang S, Marks A, Smith O, Zhang Y, et al. Phosphorylated SIRT1 associates with replication origins to prevent excess replication initiation and preserve genomic stability. Nucleic Acids Res. 2017;45:7807-7824 pubmed publisher
  • flow cytometry; mouse
Flach J, Bakker S, Mohrin M, Conroy P, Pietras E, Reynaud D, et al. Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells. Nature. 2014;512:198-202 pubmed publisher
Mersaoui S, Yu Z, Coulombe Y, Karam M, Busatto F, Masson J, et al. Arginine methylation of the DDX5 helicase RGG/RG motif by PRMT5 regulates resolution of RNA:DNA hybrids. EMBO J. 2019;38:e100986 pubmed publisher
Mohni K, Wessel S, Zhao R, Wojciechowski A, Luzwick J, Layden H, et al. HMCES Maintains Genome Integrity by Shielding Abasic Sites in Single-Strand DNA. Cell. 2019;176:144-153.e13 pubmed publisher
Oh J, Lee S, Rothstein R, Symington L. Xrs2 and Tel1 Independently Contribute to MR-Mediated DNA Tethering and Replisome Stability. Cell Rep. 2018;25:1681-1692.e4 pubmed publisher
Tubbs A, Sridharan S, van Wietmarschen N, Maman Y, Callen E, Stanlie A, et al. Dual Roles of Poly(dA:dT) Tracts in Replication Initiation and Fork Collapse. Cell. 2018;174:1127-1142.e19 pubmed publisher
Clements K, Thakar T, Nicolae C, Liang X, Wang H, Moldovan G. Loss of E2F7 confers resistance to poly-ADP-ribose polymerase (PARP) inhibitors in BRCA2-deficient cells. Nucleic Acids Res. 2018;46:8898-8907 pubmed publisher
Schleicher E, Galvan A, Imamura Kawasawa Y, Moldovan G, Nicolae C. PARP10 promotes cellular proliferation and tumorigenesis by alleviating replication stress. Nucleic Acids Res. 2018;46:8908-8916 pubmed publisher
Andrews A, McCartney H, Errington T, D Andrea A, Macara I. A senataxin-associated exonuclease SAN1 is required for resistance to DNA interstrand cross-links. Nat Commun. 2018;9:2592 pubmed publisher
Rodriguez Acebes S, Mouron S, Mendez J. Uncoupling fork speed and origin activity to identify the primary cause of replicative stress phenotypes. J Biol Chem. 2018;293:12855-12861 pubmed publisher
García Rodríguez N, Morawska M, Wong R, Daigaku Y, Ulrich H. Spatial separation between replisome- and template-induced replication stress signaling. EMBO J. 2018;37: pubmed publisher
Pan X, Drosopoulos W, Sethi L, Madireddy A, Schildkraut C, Zhang D. FANCM, BRCA1, and BLM cooperatively resolve the replication stress at the ALT telomeres. Proc Natl Acad Sci U S A. 2017;114:E5940-E5949 pubmed publisher
Tan S, Chadha S, Liu Y, Gabasova E, Perera D, Ahmed K, et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell. 2017;169:1105-1118.e15 pubmed publisher
Hodroj D, Recolin B, Serhal K, Martinez S, Tsanov N, Abou Merhi R, et al. An ATR-dependent function for the Ddx19 RNA helicase in nuclear R-loop metabolism. EMBO J. 2017;36:1182-1198 pubmed publisher
Xu H, Di Antonio M, McKinney S, Mathew V, Ho B, O Neil N, et al. CX-5461 is a DNA G-quadruplex stabilizer with selective lethality in BRCA1/2 deficient tumours. Nat Commun. 2017;8:14432 pubmed publisher
Hiraga S, Ly T, Garzón J, Horejsi Z, Ohkubo Y, Endo A, et al. Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation. EMBO Rep. 2017;18:403-419 pubmed publisher
Abraham K, Chan J, Salvi J, Ho B, Hall A, Vidya E, et al. Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids. Nucleic Acids Res. 2016;44:8870-8884 pubmed
Zhang Y, Huang L, Fu H, Smith O, Lin C, Utani K, et al. A replicator-specific binding protein essential for site-specific initiation of DNA replication in mammalian cells. Nat Commun. 2016;7:11748 pubmed publisher
Benatti P, Belluti S, Miotto B, Neusiedler J, Dolfini D, Drac M, et al. Direct non transcriptional role of NF-Y in DNA replication. Biochim Biophys Acta. 2016;1863:673-85 pubmed publisher
Gitlin A, Mayer C, Oliveira T, Shulman Z, Jones M, Koren A, et al. HUMORAL IMMUNITY. T cell help controls the speed of the cell cycle in germinal center B cells. Science. 2015;349:643-6 pubmed publisher
Fu H, Martin M, Regairaz M, Huang L, You Y, Lin C, et al. The DNA repair endonuclease Mus81 facilitates fast DNA replication in the absence of exogenous damage. Nat Commun. 2015;6:6746 pubmed publisher
Chaib Mezrag H, Lemaçon D, Fontaine H, Bellon M, Bai X, Drac M, et al. Tax impairs DNA replication forks and increases DNA breaks in specific oncogenic genome regions. Mol Cancer. 2014;13:205 pubmed publisher
Piunti A, Rossi A, Cerutti A, Albert M, Jammula S, Scelfo A, et al. Polycomb proteins control proliferation and transformation independently of cell cycle checkpoints by regulating DNA replication. Nat Commun. 2014;5:3649 pubmed publisher
Gaggioli V, Zeiser E, Rivers D, Bradshaw C, Ahringer J, Zegerman P. CDK phosphorylation of SLD-2 is required for replication initiation and germline development in C. elegans. J Cell Biol. 2014;204:507-22 pubmed publisher
Fu H, Maunakea A, Martin M, Huang L, Zhang Y, Ryan M, et al. Methylation of histone H3 on lysine 79 associates with a group of replication origins and helps limit DNA replication once per cell cycle. PLoS Genet. 2013;9:e1003542 pubmed publisher
Hua H, Namdar M, Ganier O, Gregan J, Mechali M, Kearsey S. Sequential steps in DNA replication are inhibited to ensure reduction of ploidy in meiosis. Mol Biol Cell. 2013;24:578-87 pubmed publisher
Yang J, O Donnell L, Durocher D, Brown G. RMI1 promotes DNA replication fork progression and recovery from replication fork stress. Mol Cell Biol. 2012;32:3054-64 pubmed publisher
Lana E, Megarbane A, Tourrière H, Sarda P, Lefranc G, Claustres M, et al. DNA replication is altered in Immunodeficiency Centromeric instability Facial anomalies (ICF) cells carrying DNMT3B mutations. Eur J Hum Genet. 2012;20:1044-50 pubmed publisher
Domínguez Sánchez M, Barroso S, Gómez González B, Luna R, Aguilera A. Genome instability and transcription elongation impairment in human cells depleted of THO/TREX. PLoS Genet. 2011;7:e1002386 pubmed publisher
Guo R, Chen J, Mitchell D, Johnson D. GCN5 and E2F1 stimulate nucleotide excision repair by promoting H3K9 acetylation at sites of damage. Nucleic Acids Res. 2011;39:1390-7 pubmed publisher
Ciccia A, Bredemeyer A, Sowa M, Terret M, Jallepalli P, Harper J, et al. The SIOD disorder protein SMARCAL1 is an RPA-interacting protein involved in replication fork restart. Genes Dev. 2009;23:2415-25 pubmed publisher
Stanojcic S, Lemaitre J, Brodolin K, Danis E, Mechali M. In Xenopus egg extracts, DNA replication initiates preferentially at or near asymmetric AT sequences. Mol Cell Biol. 2008;28:5265-74 pubmed publisher
Shu A, Wu C, Chen Y, Peng H, Chang H, Yew T. Evidence of DNA transfer through F-pilus channels during Escherichia coli conjugation. Langmuir. 2008;24:6796-802 pubmed publisher
Ting L, Decarie D, Ensom M. Effect of acidification on protein binding of mycophenolic acid. Ther Drug Monit. 2007;29:132-3 pubmed
product information
Catalog Number :
MAB3034
Subcategory :
Apoptosis & Cancer
Product Name :
Anti-DNA Antibody, single stranded, clone 16-19
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Host Name :
Mouse
Antigen :
DNA
Clone :
16-19
Conjugate :
Purified
Isotype :
IgG2aκ
Product Description :
Anti-DNA Antibody, single stranded, clone 16-19
Cross Reactivity :
All
Specificity :
Single stranded DNA (ssDNA) (poly-deoxythymidine). Shows 90% reactivity to ssDNA and 12% reactivity to dsDNA. ANTIGEN BINDING PROFILE: Relative Binding To: Natural DNA Synthetic homopolymers dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU) 0.03 0.84 0.02 0.11 0.03 1.00 0.03 0.53 a) Native calf thymus DNA b) Heat-denatured calf thymus DNA Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 μg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0). BINDING ASSAY Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) for one hour at room temperature, followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation. ISOELECTRIC POINT = 8.3
Package Size :
100 µg
Uses :
ELISA
Storage :
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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