NFκB Antibody, p65 subunit, active subunit, clone 12H11 | EMD Millipore MAB3026 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

NFκB Antibody, p65 subunit, active subunit, clone 12H11

catalog :

MAB3026

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

12H11

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, EMSA, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay

citations: 58

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; rat; fig 3	Kim J, Kim C, Sohn E, Kim J. Cytoplasmic translocation of high-mobility group box-1 protein is induced by diabetes and high glucose in retinal pericytes. Mol Med Rep. 2016;14:3655-61 pubmed publisher
proximity ligation assay; human; 1:100; fig 7 immunocytochemistry; human; 1:100; fig 3	Brobeil A, KÃ¤mmerer F, Tag C, Steger K, GattenlÃ¶hner S, Wimmer M. PTPIP51â€”A New RelA-tionship with the NFÎºB Signaling Pathway. Biomolecules. 2015;5:485-504 pubmed publisher
western blot; rat	PÃ©rez Vargas J, Zarco N, Vergara P, Shibayama M, Segovia J, Tsutsumi V, et al. l-Theanine prevents carbon tetrachloride-induced liver fibrosis via inhibition of nuclear factor ÎºB and down-regulation of transforming growth factor Î² and connective tissue growth factor. Hum Exp Toxicol. 2016;35:135-46 pubmed publisher
immunohistochemistry - frozen section; mouse	Zhang Y, Song C, Yuan Y, Eber A, Rodriguez Y, Levitt R, et al. Diabetic neuropathic pain development in type 2 diabetic mouse model and the prophylactic and therapeutic effects of coenzyme Q10. Neurobiol Dis. 2013;58:169-78 pubmed publisher
	Martello A, Lauriola A, Mellis D, Parish E, Dawson J, Imrie L, et al. Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy. EMBO Rep. 2020;21:e48192 pubmed publisher
	Ke W, Hong E, Saito R, Rangel M, Wang J, Viard M, et al. RNA-DNA fibers and polygons with controlled immunorecognition activate RNAi, FRET and transcriptional regulation of NF-κB in human cells. Nucleic Acids Res. 2019;47:1350-1361 pubmed publisher
	Song Z, Xiong B, Guan X, Cao F, Manyande A, Zhou Y, et al. Minocycline attenuates bone cancer pain in rats by inhibiting NF-?B in spinal astrocytes. Acta Pharmacol Sin. 2016;37:753-62 pubmed publisher
	Bandrowski A, Brush M, Grethe J, Haendel M, Kennedy D, Hill S, et al. The Resource Identification Initiative: a cultural shift in publishing. Brain Behav. 2016;6:e00417 pubmed publisher
	Bandrowski A, Brush M, Grethe J, Haendel M, Kennedy D, Hill S, et al. The Resource Identification Initiative: A cultural shift in publishing. F1000Res. 2015;4:134 pubmed publisher
	DÃaz L, MartÃnez Bonet M, SÃ¡nchez J, FernÃ¡ndez Pineda A, JimÃ©nez J, MuÃ±oz E, et al. Bryostatin activates HIV-1 latent expression in human astrocytes through a PKC and NF-Ä¸B-dependent mechanism. Sci Rep. 2015;5:12442 pubmed publisher
	Chong L, Hsu Y, Lee T, Lin Y, Chiu Y, Yang K, et al. Fluvastatin attenuates hepatic steatosis-induced fibrogenesis in rats through inhibiting paracrine effect of hepatocyte on hepatic stellate cells. BMC Gastroenterol. 2015;15:22 pubmed publisher
	Shen K, Murphy C, Chan B, Kolind M, Cheng T, Mikulec K, et al. Activated protein C (APC) can increase bone anabolism via a protease-activated receptor (PAR)1/2 dependent mechanism. J Orthop Res. 2014;32:1549-56 pubmed publisher
	Deniro M, Al Mohanna F. Nuclear factor kappa-B signaling is integral to ocular neovascularization in ischemia-independent microenvironment. PLoS ONE. 2014;9:e101602 pubmed publisher
	Sheridan G, Moeendarbary E, Pickering M, O Connor J, Murphy K. Theta-burst stimulation of hippocampal slices induces network-level calcium oscillations and activates analogous gene transcription to spatial learning. PLoS ONE. 2014;9:e100546 pubmed publisher
	Lin H, Chang Y, Lin L, Haung C, Wu C, Ou K. An Immunomodulatory Protein (Ling Zhi-8) from a Ganoderma lucidum Induced Acceleration of Wound Healing in Rat Liver Tissues after Monopolar Electrosurgery. Evid Based Complement Alternat Med. 2014;2014:916531 pubmed publisher
	Srikoon P, Kariya R, Kudo E, Goto H, Vaeteewoottacharn K, Taura M, et al. Diethyldithiocarbamate suppresses an NF-kappaB dependent metastatic pathway in cholangiocarcinoma cells. Asian Pac J Cancer Prev. 2013;14:4441-6 pubmed
	Chen C, Liu H, Chao M, Liang Y, Tsang N, Huang H, et al. NF-κB-mediated transcriptional upregulation of TNFAIP2 by the Epstein-Barr virus oncoprotein, LMP1, promotes cell motility in nasopharyngeal carcinoma. Oncogene. 2014;33:3648-59 pubmed publisher
	Deniro M, Al Mohanna F, Alsmadi O, Al Mohanna F. The nexus between VEGF and NF?B orchestrates a hypoxia-independent neovasculogenesis. PLoS ONE. 2013;8:e59021 pubmed publisher
	Macías Pérez J, Beltràn Ramírez O, Vásquez Garzón V, Salcido Neyoy M, Martínez Soriano P, Ruiz Sánchez M, et al. The effect of caffeic acid phenethyl ester analogues in a modified resistant hepatocyte model. Anticancer Drugs. 2013;24:394-405 pubmed publisher
	Chtarto A, Bockstael O, Gebara E, Vermoesen K, Melas C, Pythoud C, et al. An adeno-associated virus-based intracellular sensor of pathological nuclear factor-?B activation for disease-inducible gene transfer. PLoS ONE. 2013;8:e53156 pubmed publisher
	Aldaba Muruato L, Moreno M, Hernández Mercado E, Shibayama M, Muriel P. Secondary biliary cirrhosis in the rat is prevented by decreasing NF-?B nuclear translocation and TGF-? expression using allopurinol, an inhibitor of xanthine oxidase. Can J Physiol Pharmacol. 2012;90:1469-78 pubmed publisher
	Li X, Hopfer U, Zhuo J. Novel signaling mechanisms of intracellular angiotensin II-induced NHE3 expression and activation in mouse proximal tubule cells. Am J Physiol Renal Physiol. 2012;303:F1617-28 pubmed publisher
	Chang E, Zhang J, Sullivan S, Newman R, Singh I. N-acetylcysteine prevents preterm birth by attenuating the LPS-induced expression of contractile associated proteins in an animal model. J Matern Fetal Neonatal Med. 2012;25:2395-400 pubmed publisher
	Chavez E, Castro Sanchez L, Shibayama M, Tsutsumi V, Moreno M, Muriel P. Sulfasalazine prevents the increase in TGF-?, COX-2, nuclear NF?B translocation and fibrosis in CCl4-induced liver cirrhosis in the rat. Hum Exp Toxicol. 2012;31:913-20 pubmed
	Kim J, Kim O, Kim C, Sohn E, Jo K, Kim J. Accumulation of argpyrimidine, a methylglyoxal-derived advanced glycation end product, increases apoptosis of lens epithelial cells both in vitro and in vivo. Exp Mol Med. 2012;44:167-75 pubmed publisher
	Aldaba Muruato L, Moreno M, Shibayama M, Tsutsumi V, Muriel P. Protective effects of allopurinol against acute liver damage and cirrhosis induced by carbon tetrachloride: modulation of NF-?B, cytokine production and oxidative stress. Biochim Biophys Acta. 2012;1820:65-75 pubmed publisher
	Herkenham M, Rathore P, Brown P, Listwak S. Cautionary notes on the use of NF-?B p65 and p50 antibodies for CNS studies. J Neuroinflammation. 2011;8:141 pubmed publisher
	Chen P, Chang H, Chang J, Lin P. Aryl hydrocarbon receptor in association with RelA modulates IL-6 expression in non-smoking lung cancer. Oncogene. 2012;31:2555-65 pubmed publisher
	Hernández L, Gonzalo S, Castro M, Arruebo M, Plaza M, Murillo M, et al. Nuclear factor ?B is a key transcription factor in the duodenal contractility alterations induced by lipopolysaccharide. Exp Physiol. 2011;96:1151-62 pubmed publisher
	Ghose J, Sinha M, Das E, Jana N, Bhattacharyya N. Regulation of miR-146a by RelA/NFkB and p53 in STHdh(Q111)/Hdh(Q111) cells, a cell model of Huntington's disease. PLoS ONE. 2011;6:e23837 pubmed publisher
	Holkova B, Perkins E, Ramakrishnan V, Tombes M, Shrader E, Talreja N, et al. Phase I trial of bortezomib (PS-341; NSC 681239) and alvocidib (flavopiridol; NSC 649890) in patients with recurrent or refractory B-cell neoplasms. Clin Cancer Res. 2011;17:3388-97 pubmed publisher
	Chávez E, Castro Sanchez L, Shibayama M, Tsutsumi V, Perez Salazar E, Moreno M, et al. Effects of acetyl salycilic acid and ibuprofen in chronic liver damage induced by CCl4. J Appl Toxicol. 2012;32:51-9 pubmed publisher
	Kim J, Kim C, Sohn E, Kim J. Elevated N?-(carboxymethyl)lysine is associated with apoptosis of retinal pericytes in streptozotocin-induced diabetic rats. Ophthalmic Res. 2011;46:92-7 pubmed publisher
	Kim J, Kim O, Kim C, Kim N, Kim J. Cytotoxic role of methylglyoxal in rat retinal pericytes: Involvement of a nuclear factor-kappaB and inducible nitric oxide synthase pathway. Chem Biol Interact. 2010;188:86-93 pubmed publisher
	Lin H, Zhang L, Liu C, Xu X, Tang M, Lv H, et al. Haemin-enhanced expression of haem oxygenase-1 stabilizes erythrocyte-induced vulnerable atherosclerotic plaques. Br J Pharmacol. 2010;160:1484-95 pubmed publisher
	Jinawath N, Vasoontara C, Jinawath A, Fang X, Zhao K, Yap K, et al. Oncoproteomic analysis reveals co-upregulation of RELA and STAT5 in carboplatin resistant ovarian carcinoma. PLoS ONE. 2010;5:e11198 pubmed publisher
	Abdul Hussien H, Hanemaaijer R, Kleemann R, Verhaaren B, van Bockel J, Lindeman J. The pathophysiology of abdominal aortic aneurysm growth: corresponding and discordant inflammatory and proteolytic processes in abdominal aortic and popliteal artery aneurysms. J Vasc Surg. 2010;51:1479-87 pubmed publisher
	Rahman M, Midtling E, Svingen P, Xiong Y, Bell M, Tung J, et al. The pathogen recognition receptor NOD2 regulates human FOXP3+ T cell survival. J Immunol. 2010;184:7247-56 pubmed publisher
	Jensen K, Patel A, Larin A, Hoperia V, Saji M, Bauer A, et al. Human herpes simplex viruses in benign and malignant thyroid tumours. J Pathol. 2010;221:193-200 pubmed publisher
	Ghaly A, Marsh D. Aging-associated oxidative stress modulates the acute inflammatory response in skeletal muscle after contusion injury. Exp Gerontol. 2010;45:381-8 pubmed publisher
	Kim J, Kim C, Sohn E, Kim H, Jeong I, Kim J. Lens epithelial cell apoptosis initiates diabetic cataractogenesis in the Zucker diabetic fatty rat. Graefes Arch Clin Exp Ophthalmol. 2010;248:811-8 pubmed publisher
	O Sullivan N, Croydon L, McGettigan P, Pickering M, Murphy K. Hippocampal region-specific regulation of NF-kappaB may contribute to learning-associated synaptic reorganisation. Brain Res Bull. 2010;81:385-90 pubmed publisher
	Creus K, De Paepe B, Werbrouck B, Vervaet V, Weis J, De Bleecker J. Distribution of the NF-kappaB complex in the inflammatory exudates characterizing the idiopathic inflammatory myopathies. Ann N Y Acad Sci. 2009;1173:370-7 pubmed publisher
	Lindeman J, Abdul Hussien H, van Bockel J, Wolterbeek R, Kleemann R. Clinical trial of doxycycline for matrix metalloproteinase-9 inhibition in patients with an abdominal aneurysm: doxycycline selectively depletes aortic wall neutrophils and cytotoxic T cells. Circulation. 2009;119:2209-16 pubmed publisher
	Hallow K, Taylor W, Rachev A, Vito R. Markers of inflammation collocate with increased wall stress in human coronary arterial plaque. Biomech Model Mechanobiol. 2009;8:473-86 pubmed publisher
	Gushchina S, Leinster V, Wu D, Jasim A, Demestre M, Lopez de Heredia L, et al. Observations on the function of nuclear factor kappa B (NF-kappaB) in the survival of adult primary sensory neurons after nerve injury. Mol Cell Neurosci. 2009;40:207-16 pubmed publisher
	Janic B, Iskander A, Rad A, Soltanian Zadeh H, Arbab A. Effects of ferumoxides-protamine sulfate labeling on immunomodulatory characteristics of macrophage-like THP-1 cells. PLoS ONE. 2008;3:e2499 pubmed publisher
	Wang H, Jiang X, Xu J, Xu J, Tong J, Wang Y. The profile of gene expression and role of nuclear factor kappa B on glomerular injury in rats with Thy-1 nephritis. Clin Exp Immunol. 2008;152:559-67 pubmed publisher
	Zhang Q, Hamajima Y, Zhang Q, Lin J. Identification of Id1 in acquired middle ear cholesteatoma. Arch Otolaryngol Head Neck Surg. 2008;134:306-10 pubmed publisher
	Bush A, Hyson R. Effects of lithium and deafferentation on expression of glycogen synthase kinase-3beta, NFkappaB, beta-catenin and pCreb in the chick cochlear nucleus. Brain Res. 2008;1203:18-25 pubmed publisher
	Sheridan G, Pickering M, Twomey C, Moynagh P, O Connor J, Murphy K. NF-kappaB activity in distinct neural subtypes of the rat hippocampus: Influence of time and GABA antagonism in acute slice preparations. Learn Mem. 2007;14:525-32 pubmed
	O Sullivan N, McGettigan P, Sheridan G, Pickering M, Conboy L, O Connor J, et al. Temporal change in gene expression in the rat dentate gyrus following passive avoidance learning. J Neurochem. 2007;101:1085-98 pubmed
	Mitsiades C, McMillin D, Kotoula V, Poulaki V, McMullan C, Negri J, et al. Antitumor effects of the proteasome inhibitor bortezomib in medullary and anaplastic thyroid carcinoma cells in vitro. J Clin Endocrinol Metab. 2006;91:4013-21 pubmed
	Mitsiades C, Kotoula V, Poulaki V, Sozopoulos E, Negri J, Charalambous E, et al. Epidermal growth factor receptor as a therapeutic target in human thyroid carcinoma: mutational and functional analysis. J Clin Endocrinol Metab. 2006;91:3662-6 pubmed
	Guo J, Cho C, Wang J, Koo M. Differential effects of selective and non-selective inhibition of nitric oxide synthase on the expression and activity of cyclooxygenase-2 during gastric ulcer healing. Eur J Pharmacol. 2006;536:301-8 pubmed
	Bodger K, Halfvarson J, Dodson A, Campbell F, Wilson S, Lee R, et al. Altered colonic glycoprotein expression in unaffected monozygotic twins of inflammatory bowel disease patients. Gut. 2006;55:973-7 pubmed
	Ueda Y, Nakagawa T, Kubota T, Ido K, Sato K. Glioma cells under hypoxic conditions block the brain microvascular endothelial cell death induced by serum starvation. J Neurochem. 2005;95:99-110 pubmed
	Guthmann F, Wissel H, Schachtrup C, Tölle A, Rudiger M, Spener F, et al. Inhibition of TNFalpha in vivo prevents hyperoxia-mediated activation of caspase 3 in type II cells. Respir Res. 2005;6:10 pubmed

product information

Catalog Number :

MAB3026

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-NFκB Antibody, p65 subunit, active subunit, clone 12H11

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q04206

Host Name :

Mouse

Antigen :

NFκB

Clone :

12H11

Conjugate :

Purified

Isotype :

IgG3

Product Description :

Anti-NFκB Antibody, p65 subunit, active subunit, clone 12H11

Cross Reactivity :

Human;Mouse;Rat;Rabbit

Background :

The transcription factor NFkappaB (Nuclear Factor kappa B) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFkappaB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFkappaB1 (p50 and its precursor p105), and NFkappaB2 (p52 and its precursor p100). NFkappaB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFkappaB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFkappaB is the most abundant in cells.

ALT Names :

Rel A

Immunogen :

Peptide corresponding to human p65 coupled to BSA.

Specificity :

Recognizes an epitope overlapping the nuclear location signal (NLS) of the p65 subunit of the NFkB heterodimer. Thus it selectively binds to the activated form of NFkB (Zabel et al., 1993). The antibody can also be used for electrophoretic mobility supershift assays (EMSA). The nuclear factor kB (NFkB) is a sequence-specific DNA-binding protein (Sen & Baltimore, 1986). It is a pleiotropic transcription factor which is involved in the expression of a variety of cellular and viral genes (Lenardo & Baltimore, 1989). NFkB consists of two subunits which are named according to their molecular weight, p50 and p65 (Kawakami et al., 1988). The subunits are stabilized by a so-called inhibitory chain IkB (Baeuerle & Baltimore, 1988). In quiescent cells, NFkB resides in the cytosol in an inactive form which can be activated in vivo by treatment of cells with cytokines or protein kinase activators. In vitro, it is possible to generate the active form of NFkB by treatment with sodium deoxycholate, formadine or electrophoretic size fractionation. The active form of NFkB is a heterotetrameric protein, consisting of the two p50 and two p65 subunits (Lenardo et al., 1987). After activation NFkB translocates from the cytosol to the nucleus of the cell, binds to specific DNA sequences and initiates transcription.

Package Size :

100 µg

Uses :

Electrophoretic Mobility Shift Assay;Flow Cytometry;Immunocytochemistry;Immunofluorescence;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Stable for 6 months at 2-8ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.