Kindlin-2 Antibody, clone 3A3 | EMD Millipore MAB2617 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Kindlin-2 Antibody, clone 3A3

catalog :

MAB2617

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3A3

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation, immunohistochemistry knockout validation

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry knockout validation; mouse; loading ...; fig 5e immunohistochemistry - paraffin section; mouse; loading ...; fig 5e immunocytochemistry; rat; 1:200; loading ...; fig 3e western blot; rat; loading ...; fig 1c	Qi L, Chi X, Zhang X, Feng X, Chu W, Zhang S, et al. Kindlin-2 suppresses transcription factor GATA4 through interaction with SUV39H1 to attenuate hypertrophy. Cell Death Dis. 2019;10:890 pubmed publisher
western blot knockout validation; mouse; 1:1000; loading ...; fig e2e	El Brolosy M, Kontarakis Z, Rossi A, Kuenne C, Günther S, Fukuda N, et al. Genetic compensation triggered by mutant mRNA degradation. Nature. 2019;: pubmed publisher
western blot knockout validation; mouse; 1:1000; fig 1 immunocytochemistry; mouse; 1:500; fig 3	Theodosiou M, Widmaier M, BÃ¶ttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher
immunocytochemistry; human; 1:200; loading ...; fig 5E western blot; human; 1:1000; loading ...; fig 2A immunohistochemistry; mouse; 1:200; loading ...; fig 8B	Wei X, Wang X, Zhan J, Chen Y, Fang W, Zhang L, et al. Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation. J Cell Biol. 2017;216:1455-1471 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig s1d	Bharadwaj M, Strohmeyer N, Colo G, Helenius J, Beerenwinkel N, Schiller H, et al. αV-class integrins exert dual roles on α5β1 integrins to strengthen adhesion to fibronectin. Nat Commun. 2017;8:14348 pubmed publisher
immunocytochemistry; human; 1:200; fig 3a	Zeng F, Xie Y, Liao L, Li L, Chen B, Xie J, et al. Biological characterization of three immortalized esophageal epithelial cell lines. Mol Med Rep. 2016;14:4802-4810 pubmed publisher
immunocytochemistry; human; 1:400; fig 4	Austen K, Ringer P, Mehlich A, Chrostek Grashoff A, Kluger C, Klingner C, et al. Extracellular rigidity sensing by talin isoform-specific mechanical linkages. Nat Cell Biol. 2015;17:1597-606 pubmed publisher
immunoprecipitation; rat immunocytochemistry; rat western blot; rat	Qi L, Yu Y, Chi X, Xu W, Lu D, Song Y, et al. Kindlin-2 interacts with Î±-actinin-2 and Î²1 integrin to maintain the integrity of the Z-disc in cardiac muscles. FEBS Lett. 2015;589:2155-62 pubmed publisher
western blot; mouse; fig 9	Yazlovitskaya E, Tseng H, Viquez O, Tu T, Mernaugh G, McKee K, et al. Integrin Î±3Î²1 regulates kidney collecting duct development via TRAF6-dependent K63-linked polyubiquitination of Akt. Mol Biol Cell. 2015;26:1857-74 pubmed publisher
immunocytochemistry; human; fig 5a	Artym V, Swatkoski S, Matsumoto K, Campbell C, Petrie R, Dimitriadis E, et al. Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network. J Cell Biol. 2015;208:331-50 pubmed publisher
	Kawamura E, Hamilton G, Miskiewicz E, MacPhee D. Fermitin family homolog-2 (FERMT2) is highly expressed in human placental villi and modulates trophoblast invasion. BMC Dev Biol. 2018;18:19 pubmed publisher
	Mana G, Clapero F, Panieri E, Panero V, Böttcher R, Tseng H, et al. PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis. Nat Commun. 2016;7:13546 pubmed publisher
	Yang M, Du J, Lu D, Ren C, Shen H, Qiao J, et al. Increased expression of kindlin 2 in luteinized granulosa cells correlates with androgen receptor level in patients with polycystic ovary syndrome having hyperandrogenemia. Reprod Sci. 2014;21:696-703 pubmed publisher

product information

Catalog Number :

MAB2617

Subcategory :

Cell Structure

Product Name :

Anti-Kindlin-2 Antibody, clone 3A3

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q96AC1

Host Name :

Mouse

Antigen :

Kindlin-2

Clone :

3A3

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Kindlin-2 Antibody, clone 3A3

Cross Reactivity :

Human;Mouse;Rat;Bovine

Background :

The Kindlin family is a group of focal adhesion proteins that have become a point of interest because of their ability to bind and activate integrins. Abnormalities in this ability have linked Kindlin to several diseases, both inherited and acquired, such as Kindler syndrome, cancer, and leukocyte adhesion deficiency. The isoform Kindlin-2 specifically participates in the recruitment of migfilin protein to focal adhesion sites for cell-ECM interactions, coordinates the manufacture of actin and modifications to cell shape. It is also actively involved in connecting ECM adhesion sites and the actin cytoskeleton (Shi, 2007). Kindlin-2 is pervasively expressed throughout tissue.

ALT Names :

Mitogen-inducible gene 2 protein;Pleckstrin homology domain-containing family C member 1;fermitin family homolog 2;kindlin 2;mitogen inducible gene 2 protein

Immunogen :

GST-tagged recombinant protein corresponding to human Kindlin-2.

Specificity :

The antibody recognizes Kindlin-2.

Package Size :

100 μg

Uses :

Western Blotting;Immunocytochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt