This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
GluR1-NT (NT) Antibody, clone RH95
catalog :
MAB2263
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
RH95

The same clone is also sold as:
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
citations: 52
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig 2e
Han W, Li J, Pelkey K, Pandey S, Chen X, Wang Y, et al. Shisa7 is a GABAA receptor auxiliary subunit controlling benzodiazepine actions. Science. 2019;366:246-250 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 4b
Duan J, Pandey S, Li T, Castellano D, Gu X, Li J, et al. Genetic Deletion of GABAA Receptors Reveals Distinct Requirements of Neurotransmitter Receptors for GABAergic and Glutamatergic Synapse Development. Front Cell Neurosci. 2019;13:217 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4g
Zhong L, Xu Y, Zhuo R, Wang T, Wang K, Huang R, et al. Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model. Nat Commun. 2019;10:1365 pubmed publisher
  • immunocytochemistry; rat; 1:300; loading ...; fig 3a
Gao R, Zaccard C, Shapiro L, Dionisio L, Martín de Saavedra M, Piguel N, et al. The CNTNAP2-CASK complex modulates GluA1 subcellular distribution in interneurons. Neurosci Lett. 2019;701:92-99 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s2a
  • western blot; human; 1:1000; loading ...; fig 1c
  • western blot; mouse; 1:1000; loading ...; fig 1d
Seo B, Cho T, Lee D, Lee J, Lee B, Kim S, et al. LARGE, an intellectual disability-associated protein, regulates AMPA-type glutamate receptor trafficking and memory. Proc Natl Acad Sci U S A. 2018;115:7111-7116 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s6c
Wang Y, Figueiredo D, Sun X, Dong Z, Chen W, Cui W, et al. Controlling of glutamate release by neuregulin3 via inhibiting the assembly of the SNARE complex. Proc Natl Acad Sci U S A. 2018;115:2508-2513 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 9g
  • western blot; mouse; loading ...; fig 9k
Niu Y, Dai Z, Liu W, Zhang C, Yang Y, Guo Z, et al. Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory. elife. 2017;6: pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 1b
Ouyang J, Carcea I, Schiavo J, Jones K, Rabinowitsch A, Kolaric R, et al. Food restriction induces synaptic incorporation of calcium-permeable AMPA receptors in nucleus accumbens. Eur J Neurosci. 2017;45:826-836 pubmed publisher
  • western blot; mouse; 1:1000; fig 7a
Shih Y, Hsueh Y. VCP and ATL1 regulate endoplasmic reticulum and protein synthesis for dendritic spine formation. Nat Commun. 2016;7:11020 pubmed publisher
  • western blot; rat; fig 4
Gu X, Mao X, Lussier M, Hutchison M, Zhou L, Hamra F, et al. GSG1L suppresses AMPA receptor-mediated synaptic transmission and uniquely modulates AMPA receptor kinetics in hippocampal neurons. Nat Commun. 2016;7:10873 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 2
  • western blot; mouse; fig 2
Li W, Xu X, Pozzo Miller L. Excitatory synapses are stronger in the hippocampus of Rett syndrome mice due to altered synaptic trafficking of AMPA-type glutamate receptors. Proc Natl Acad Sci U S A. 2016;113:E1575-84 pubmed publisher
  • western blot; mouse; fig 2
Mei Y, Monteiro P, Zhou Y, Kim J, Gao X, Fu Z, et al. Adult restoration of Shank3 expression rescues selective autistic-like phenotypes. Nature. 2016;530:481-4 pubmed publisher
  • western blot; mouse; fig s1
Kim G, Luján R, Schwenk J, Kelley M, Aguado C, Watanabe M, et al. Membrane palmitoylated protein 2 is a synaptic scaffold protein required for synaptic SK2-containing channel function. elife. 2016;5: pubmed publisher
  • immunocytochemistry; rat; 1:100; fig s3
Stanic J, Carta M, Eberini I, Pelucchi S, Marcello E, Genazzani A, et al. Rabphilin 3A retains NMDA receptors at synaptic sites through interaction with GluN2A/PSD-95 complex. Nat Commun. 2015;6:10181 pubmed publisher
  • western blot; human; 1:1500; fig s5
Farrow P, Khodosevich K, Sapir Y, Schulmann A, Aslam M, Stern Bach Y, et al. Auxiliary subunits of the CKAMP family differentially modulate AMPA receptor properties. elife. 2015;4:e09693 pubmed publisher
  • western blot; mouse; 1:2000; fig 1
Zhang P, Fu W, Fu A, Ip N. S-nitrosylation-dependent proteasomal degradation restrains Cdk5 activity to regulate hippocampal synaptic strength. Nat Commun. 2015;6:8665 pubmed publisher
  • western blot; mouse; 1:1000
Atkin G, Moore S, Lu Y, Nelson R, Tipper N, Rajpal G, et al. Loss of F-box only protein 2 (Fbxo2) disrupts levels and localization of select NMDA receptor subunits, and promotes aberrant synaptic connectivity. J Neurosci. 2015;35:6165-78 pubmed publisher
  • western blot; rat; 1:1500
Peng X, Lister A, Rabinowitsch A, Kolaric R, Cabeza de Vaca S, Ziff E, et al. Episodic sucrose intake during food restriction increases synaptic abundance of AMPA receptors in nucleus accumbens and augments intake of sucrose following restoration of ad libitum feeding. Neuroscience. 2015;295:58-71 pubmed publisher
  • immunoprecipitation; rat; 1:10; fig 4
  • immunocytochemistry; rat; 1:500; fig 6
  • western blot; rat; 1:1000; fig 1
Garcia Alvarez G, Lu B, Yap K, Wong L, Thevathasan J, Lim L, et al. STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs. Mol Biol Cell. 2015;26:1141-59 pubmed publisher
  • western blot; rat; 1:1000
Zheng D, Cabeza de Vaca S, Jurkowski Z, Carr K. Nucleus accumbens AMPA receptor involvement in cocaine-conditioned place preference under different dietary conditions in rats. Psychopharmacology (Berl). 2015;232:2313-22 pubmed publisher
  • immunohistochemistry; rat
Nakano Kobayashi A, Tai Y, Nadif Kasri N, Van Aelst L. The X-linked mental retardation protein OPHN1 interacts with Homer1b/c to control spine endocytic zone positioning and expression of synaptic potentiation. J Neurosci. 2014;34:8665-71 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:1000
Gong K, Kung L, Magni G, Bhargava A, Jasmin L. Increased response to glutamate in small diameter dorsal root ganglion neurons after sciatic nerve injury. PLoS ONE. 2014;9:e95491 pubmed publisher
  • immunocytochemistry; mouse
Sullivan S, Dillon G, Sullivan J, Ho A. Mint proteins are required for synaptic activity-dependent amyloid precursor protein (APP) trafficking and amyloid ? generation. J Biol Chem. 2014;289:15374-83 pubmed publisher
  • immunoprecipitation; mouse
  • western blot; mouse
Copits B, Swanson G. Kainate receptor post-translational modifications differentially regulate association with 4.1N to control activity-dependent receptor endocytosis. J Biol Chem. 2013;288:8952-65 pubmed publisher
Royo M, Gutiérrez Y, Fernandez Monreal M, Gutiérrez Eisman S, Jimenez R, Jurado S, et al. A retention-release mechanism based on RAB11FIP2 for AMPA receptor synaptic delivery during long-term potentiation. J Cell Sci. 2019;132: pubmed publisher
Mitroi D, Pereyra Gómez G, Soto Huelin B, Senovilla F, Kobayashi T, Esteban J, et al. NPC1 enables cholesterol mobilization during long-term potentiation that can be restored in Niemann-Pick disease type C by CYP46A1 activation. EMBO Rep. 2019;20:e48143 pubmed publisher
Gobbo F, Marchetti L, Jacob A, Pinto B, Binini N, Pecoraro Bisogni F, et al. Activity-dependent expression of Channelrhodopsin at neuronal synapses. Nat Commun. 2017;8:1629 pubmed publisher
Farhy Tselnicker I, van Casteren A, Lee A, Chang V, Aricescu A, Allen N. Astrocyte-Secreted Glypican 4 Regulates Release of Neuronal Pentraxin 1 from Axons to Induce Functional Synapse Formation. Neuron. 2017;96:428-445.e13 pubmed publisher
Korb E, Herre M, Zucker Scharff I, Gresack J, Allis C, Darnell R. Excess Translation of Epigenetic Regulators Contributes to Fragile X Syndrome and Is Alleviated by Brd4 Inhibition. Cell. 2017;170:1209-1223.e20 pubmed publisher
Zhang B, Lu W. Src homology 2 domain-containing phosphotyrosine phosphatase 2 (Shp2) controls surface GluA1 protein in synaptic homeostasis. J Biol Chem. 2017;292:15481-15488 pubmed publisher
Szíber Z, Liliom H, Morales C, Ignácz A, Rátkai A, Ellwanger K, et al. Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover. Mol Biol Cell. 2017;28:285-295 pubmed publisher
Song R, Tolentino R, Sobie E, Neves Zaph S. Cross-regulation of Phosphodiesterase 1 and Phosphodiesterase 2 Activities Controls Dopamine-mediated Striatal ?-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Trafficking. J Biol Chem. 2016;291:23257-23267 pubmed
Burgdorf J, Zhang X, Colechio E, Ghoreishi Haack N, Gross A, Kroes R, et al. Insulin-Like Growth Factor I Produces an Antidepressant-Like Effect and Elicits N-Methyl-D-Aspartate Receptor Independent Long-Term Potentiation of Synaptic Transmission in Medial Prefrontal Cortex and Hippocampus. Int J Neuropsychopharmacol. 2015;19: pubmed publisher
Garcia Alvarez G, Shetty M, Lu B, Yap K, Oh Hora M, Sajikumar S, et al. Impaired spatial memory and enhanced long-term potentiation in mice with forebrain-specific ablation of the Stim genes. Front Behav Neurosci. 2015;9:180 pubmed publisher
Mignogna M, Giannandrea M, Gurgone A, Fanelli F, Raimondi F, Mapelli L, et al. The intellectual disability protein RAB39B selectively regulates GluA2 trafficking to determine synaptic AMPAR composition. Nat Commun. 2015;6:6504 pubmed publisher
Lussier M, Gu X, Lu W, Roche K. Casein kinase 2 phosphorylates GluA1 and regulates its surface expression. Eur J Neurosci. 2014;39:1148-58 pubmed publisher
Wang J, Bina R, Wingard J, Terwilliger E, Hammer R, Nikulina E. Knockdown of tropomyosin-related kinase B receptor expression in the nucleus accumbens shell prevents intermittent social defeat stress-induced cross-sensitization to amphetamine in rats. Eur J Neurosci. 2014;39:1009-17 pubmed publisher
Balasuriya D, Goetze T, Barrera N, Stewart A, Suzuki Y, Edwardson J. ?-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptors adopt different subunit arrangements. J Biol Chem. 2013;288:21987-98 pubmed publisher
Salussolia C, Gan Q, Kazi R, Singh P, Allopenna J, Furukawa H, et al. A eukaryotic specific transmembrane segment is required for tetramerization in AMPA receptors. J Neurosci. 2013;33:9840-5 pubmed publisher
Raynaud F, Janossy A, Dahl J, Bertaso F, Perroy J, Varrault A, et al. Shank3-Rich2 interaction regulates AMPA receptor recycling and synaptic long-term potentiation. J Neurosci. 2013;33:9699-715 pubmed publisher
Incontro S, Ciruela F, ZIFF E, Hofmann F, Sánchez Prieto J, Torres M. The type II cGMP dependent protein kinase regulates GluA1 levels at the plasma membrane of developing cerebellar granule cells. Biochim Biophys Acta. 2013;1833:1820-31 pubmed publisher
Caba ero D, Baker A, Zhou S, Hargett G, Irie T, Xia Y, et al. Pain after discontinuation of morphine treatment is associated with synaptic increase of GluA4-containing AMPAR in the dorsal horn of the spinal cord. Neuropsychopharmacology. 2013;38:1472-84 pubmed publisher
Zheng D, Liu S, Cabeza de Vaca S, Carr K. Effects of time of feeding on psychostimulant reward, conditioned place preference, metabolic hormone levels, and nucleus accumbens biochemical measures in food-restricted rats. Psychopharmacology (Berl). 2013;227:307-20 pubmed publisher
Burgdorf J, Zhang X, Nicholson K, Balster R, Leander J, Stanton P, et al. GLYX-13, a NMDA receptor glycine-site functional partial agonist, induces antidepressant-like effects without ketamine-like side effects. Neuropsychopharmacology. 2013;38:729-42 pubmed publisher
Havekes R, Canton D, Park A, Huang T, Nie T, Day J, et al. Gravin orchestrates protein kinase A and ?2-adrenergic receptor signaling critical for synaptic plasticity and memory. J Neurosci. 2012;32:18137-49 pubmed publisher
Zhong X, Li H, Chang Q. MeCP2 phosphorylation is required for modulating synaptic scaling through mGluR5. J Neurosci. 2012;32:12841-7 pubmed publisher
Corthell J, Fadool D, Trombley P. Connexin and AMPA receptor expression changes over time in the rat olfactory bulb. Neuroscience. 2012;222:38-48 pubmed publisher
Lee D, CHUNG J, Chung K, Kang M. Reactive oxygen species (ROS) modulate AMPA receptor phosphorylation and cell-surface localization in concert with pain-related behavior. Pain. 2012;153:1905-15 pubmed publisher
Kang M, Nuriya M, Guo Y, Martindale K, Lee D, Huganir R. Proteomic analysis of ?-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor complexes. J Biol Chem. 2012;287:28632-45 pubmed publisher
Liu S, Zheng D, Peng X, Cabeza de Vaca S, Carr K. Enhanced cocaine-conditioned place preference and associated brain regional levels of BDNF, p-ERK1/2 and p-Ser845-GluA1 in food-restricted rats. Brain Res. 2011;1400:31-41 pubmed publisher
Peng X, Ziff E, Carr K. Effects of food restriction and sucrose intake on synaptic delivery of AMPA receptors in nucleus accumbens. Synapse. 2011;65:1024-31 pubmed publisher
Ferrario C, Loweth J, Milovanovic M, Ford K, Galiñanes G, Heng L, et al. Alterations in AMPA receptor subunits and TARPs in the rat nucleus accumbens related to the formation of Ca²?-permeable AMPA receptors during the incubation of cocaine craving. Neuropharmacology. 2011;61:1141-51 pubmed publisher
product information
Catalog Number :
MAB2263
Subcategory :
Neuroscience
Product Name :
Anti-GluR1-NT (NT) Antibody, clone RH95
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P19490
Host Name :
Mouse
Antigen :
GluR1-NT (NT)
Clone :
RH95
Conjugate :
Purified
Isotype :
IgG2aκ
Product Description :
Anti-GluR1-NT (NT) Antibody, clone RH95
Cross Reactivity :
Rat
Background :
Glutamate receptors (GluRs) are a diverse group responsible for mediating most of the excitatory synaptic transmission in the CNS of vertebrates. They can be categorized as ionotropic or metabotropic and subcategorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this position is occupied by a Gln residue. The insertion or removal of GluR1/GluR4 oligomeric channels from postsynaptic membranes appears to be LTP/LTD activity dependent while GluR2/GluR3 oligomers are continuously cycling.
ALT Names :
AMPA 1;AMPA-selective glutamate receptor 1;Glutamate receptor ionotropic, AMPA 1;glutamate receptor, ionotropic, AMPA 1
Immunogen :
Thyroglobulin-conjugated linear peptide of rat GluR1 designed around the N-terminus.
Specificity :
This antibody recognizes GluR1 at the N-terminus.
Package Size :
100 μg
Uses :
Western Blotting;Immunoprecipitation;Immunocytochemistry;ELISA
Storage :
Stable for 1 year at 2-8°C from date of receipt
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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