Integrin β1 Antibody, clone 12G10 | EMD Millipore MAB2247-I product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Integrin β1 Antibody, clone 12G10

catalog :

MAB2247-I

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

12G10

The same clone is also sold as:

Novus Biologicals: NB100-63255
Santa Cruz Biotechnology: sc-59827
OriGene: SM1740P, SM1740PT, TA353269L
Absolute Antibody: Ab00849-13.12, Ab00849-10.3, Ab00849-23.0, Ab00849-10.0, Ab00849-1.1
Bio-Rad: MCA2028T, MCA2028, MCA2028A647, MCA2028F, MCA2028PE
Abcam: ab202641, ab30394
Invitrogen: MA1-80764
LifeSpan Biosciences: LS-C43968

reactivity :

human

application :

western blot, immunocytochemistry, flow cytometry

citations: 19

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; fig 4	HÃ¸ye A, Couchman J, Wewer U, Yoneda A. The Phosphorylation and Distribution of Cortactin Downstream of Integrin Î±9Î²1 Affects Cancer Cell Behaviour. Sci Rep. 2016;6:28529 pubmed publisher
immunocytochemistry; human; loading ...; fig 6d	Romagnoli M, Mineva N, Polmear M, Conrad C, Srinivasan S, Loussouarn D, et al. ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis. EMBO Mol Med. 2014;6:278-94 pubmed publisher
flow cytometry; human; 1:150 western blot; human; 1:150; fig 2f	Valtcheva N, Primorac A, Jurisic G, Hollmen M, Detmar M. The orphan adhesion G protein-coupled receptor GPR97 regulates migration of lymphatic endothelial cells via the small GTPases RhoA and Cdc42. J Biol Chem. 2013;288:35736-48 pubmed publisher
western blot; human	Costa P, Scales T, Ivaska J, Parsons M. Integrin-specific control of focal adhesion kinase and RhoA regulates membrane protrusion and invasion. PLoS ONE. 2013;8:e74659 pubmed publisher
flow cytometry; human	Kligys K, Wu Y, Hamill K, Lewandowski K, Hopkinson S, Budinger G, et al. Laminin-332 and ?3?1 integrin-supported migration of bronchial epithelial cells is modulated by fibronectin. Am J Respir Cell Mol Biol. 2013;49:731-40 pubmed publisher
	Markus B, Bell G, Lorenzi H, Lourido S. Optimizing Systems for Cas9 Expression in Toxoplasma gondii. mSphere. 2019;4: pubmed publisher
	Kam C, Dubash A, Magistrati E, Polo S, Satchell K, Sheikh F, et al. Desmoplakin maintains gap junctions by inhibiting Ras/MAPK and lysosomal degradation of connexin-43. J Cell Biol. 2018;217:3219-3235 pubmed publisher
	Cook J, Ueno N, Lodoen M. Toxoplasma gondii disrupts β1 integrin signaling and focal adhesion formation during monocyte hypermotility. J Biol Chem. 2018;293:3374-3385 pubmed publisher
	Kumar R, Singh N, Abdin M, Patel A, Medigeshi G. Dengue Virus Capsid Interacts with DDX3X-A Potential Mechanism for Suppression of Antiviral Functions in Dengue Infection. Front Cell Infect Microbiol. 2017;7:542 pubmed publisher
	Mathew S, Palamuttam R, Mernaugh G, Ramalingam H, Lu Z, Zhang M, et al. Talin regulates integrin β1-dependent and -independent cell functions in ureteric bud development. Development. 2017;144:4148-4158 pubmed publisher
	Zhang G, Gu Y, Begum R, Chen H, Gao X, McGrath J, et al. Kindlin-1 Regulates Keratinocyte Electrotaxis. J Invest Dermatol. 2016;136:2229-2239 pubmed publisher
	Woskowicz A, Weaver S, Shitomi Y, Ito N, Itoh Y. MT-LOOP-dependent localization of membrane type I matrix metalloproteinase (MT1-MMP) to the cell adhesion complexes promotes cancer cell invasion. J Biol Chem. 2013;288:35126-37 pubmed publisher
	Rajshankar D, Downey G, McCulloch C. IL-1? enhances cell adhesion to degraded fibronectin. FASEB J. 2012;26:4429-44 pubmed publisher
	Ai J, Xin X, Zheng M, Wang S, Peng S, Li J, et al. A triad of lys12, lys41, arg78 spatial domain, a novel identified heparin binding site on tat protein, facilitates tat-driven cell adhesion. PLoS ONE. 2008;3:e2662 pubmed publisher
	Azab A, Quang P, Azab F, Pitsillides C, Thompson B, Chonghaile T, et al. P-selectin glycoprotein ligand regulates the interaction of multiple myeloma cells with the bone marrow microenvironment. Blood. 2012;119:1468-78 pubmed publisher
	Quast T, Eppler F, Semmling V, Schild C, Homsi Y, Levy S, et al. CD81 is essential for the formation of membrane protrusions and regulates Rac1-activation in adhesion-dependent immune cell migration. Blood. 2011;118:1818-27 pubmed publisher
	Gu Z, Noss E, Hsu V, Brenner M. Integrins traffic rapidly via circular dorsal ruffles and macropinocytosis during stimulated cell migration. J Cell Biol. 2011;193:61-70 pubmed publisher
	Lacroix B, Van Dijk J, Gold N, Guizetti J, Aldrian Herrada G, Rogowski K, et al. Tubulin polyglutamylation stimulates spastin-mediated microtubule severing. J Cell Biol. 2010;189:945-54 pubmed publisher
	Bass R, Wagstaff L, Ravenhill L, Ellis V. Binding of extracellular maspin to beta1 integrins inhibits vascular smooth muscle cell migration. J Biol Chem. 2009;284:27712-20 pubmed publisher

product information

Catalog Number :

MAB2247-I

Subcategory :

Cell Structure

Product Name :

Anti-Integrin β1 Antibody, clone 12G10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P05556

Host Name :

Mouse

Antigen :

Integrin β1

Clone :

12G10

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Integrin β1 Antibody, clone 12G10

Cross Reactivity :

Human

Background :

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

ALT Names :

Integrin beta-1;Fibronectin receptor subunit beta;VLA-4 subunit beta;CD29

Immunogen :

Purified human β1 Integrin preparation from HT1080 fibrosarcoma cell extract

Package Size :

100 μg

Uses :

Western Blotting;Flow Cytometry;Immunofluorescence

Storage :

Stable for 1 year at 2-8°C from date of receipt