β-Catenin Antibody, clone 5H10 | EMD Millipore MAB2081 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

β-Catenin Antibody, clone 5H10

catalog :

MAB2081

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5H10

The same clone is also sold as:

Abnova: MAB14490, MAB14746

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:200; fig s8 western blot; human; fig 5	Sellerio A, Ciusani E, Ben Moshe N, Coco S, Piccinini A, Myers C, et al. Overshoot during phenotypic switching of cancer cell populations. Sci Rep. 2015;5:15464 pubmed publisher
western blot; mouse; 1:1000	Rutkowska Wlodarczyk I, Aller M, Valbuena S, Bologna J, PrÃ©zeau L, Lerma J. A proteomic analysis reveals the interaction of GluK1 ionotropic kainate receptor subunits with Go proteins. J Neurosci. 2015;35:5171-9 pubmed publisher
immunocytochemistry; human; 1:500	Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, et al. Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways. PLoS ONE. 2014;9:e104937 pubmed publisher
immunocytochemistry; human western blot; human; fig s1	Knoblich K, Wang H, Sharma C, Fletcher A, Turley S, Hemler M. Tetraspanin TSPAN12 regulates tumor growth and metastasis and inhibits ?-catenin degradation. Cell Mol Life Sci. 2014;71:1305-14 pubmed publisher
	Manso C, Querol L, Lleixà C, Poncelet M, Mekaouche M, Vallat J, et al. Anti-Neurofascin-155 IgG4 antibodies prevent paranodal complex formation in vivo. J Clin Invest. 2019;129:2222-2236 pubmed publisher
	Gurney A, Axelrod F, Bond C, Cain J, Chartier C, Donigan L, et al. Wnt pathway inhibition via the targeting of Frizzled receptors results in decreased growth and tumorigenicity of human tumors. Proc Natl Acad Sci U S A. 2012;109:11717-22 pubmed publisher
	Wan X, Zhao Y, Fan X, Cai H, Zhang Y, Chen M, et al. Molecular prognostic prediction for locally advanced nasopharyngeal carcinoma by support vector machine integrated approach. PLoS ONE. 2012;7:e31989 pubmed publisher
	Belgiovine C, Chiodi I, Mondello C. Relocalization of cell adhesion molecules during neoplastic transformation of human fibroblasts. Int J Oncol. 2011;39:1199-204 pubmed publisher
	Sablina A, Hector M, Colpaert N, Hahn W. Identification of PP2A complexes and pathways involved in cell transformation. Cancer Res. 2010;70:10474-84 pubmed publisher
	Cowden Dahl K, Symowicz J, Ning Y, Gutierrez E, Fishman D, Adley B, et al. Matrix metalloproteinase 9 is a mediator of epidermal growth factor-dependent e-cadherin loss in ovarian carcinoma cells. Cancer Res. 2008;68:4606-13 pubmed publisher
	Giuliani N, Morandi F, Tagliaferri S, Lazzaretti M, Donofrio G, Bonomini S, et al. Production of Wnt inhibitors by myeloma cells: potential effects on canonical Wnt pathway in the bone microenvironment. Cancer Res. 2007;67:7665-74 pubmed
	Zeineldin R, Rosenberg M, Ortega D, Buhr C, Chavez M, Stack M, et al. Mesenchymal transformation in epithelial ovarian tumor cells expressing epidermal growth factor receptor variant III. Mol Carcinog. 2006;45:851-60 pubmed
	Rieske P, Krynska B, Azizi S. Human fibroblast-derived cell lines have characteristics of embryonic stem cells and cells of neuro-ectodermal origin. Differentiation. 2005;73:474-83 pubmed
	Lee N, Mruk D, Wong C, Cheng C. Regulation of Sertoli-germ cell adherens junction dynamics in the testis via the nitric oxide synthase (NOS)/cGMP/protein kinase G (PRKG)/beta-catenin (CATNB) signaling pathway: an in vitro and in vivo study. Biol Reprod. 2005;73:458-71 pubmed

product information

Catalog Number :

MAB2081

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-β-Catenin Antibody, clone 5H10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P35222

Host Name :

Mouse

Antigen :

β-Catenin

Clone :

5H10

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-β-Catenin Antibody, clone 5H10

Cross Reactivity :

Human;Rat

Background :

The transmembrane, calcium dependent molecule E-cadherin is considered to be one of the key molecules in the formation of the intercellular junctional complex and establishment of polarity in epithelial cells. Catenins, the alpha-subunit (102 kDa), beta-subunit (88 kDa) and gamma-subunit (82 kDa), [reviewed in Takeichi, 1991] are a group of proteins that interact with the intercellular domain of E-cadherin, resulting in complexes of E-cadherin/beta-catenin/alpha-catenin or E-cadherin/gamma-catenin/alpha-Catenin (Hinck et al., 1994). Beta-catenin, the vertebrate homologue of Drosophila segment polarity gene armadillo, and key element of the Wnt signal transduction pathways, is important in embryogenesis, cell transformation, and intercellular adhesion including the adhesion, motility and metastasis of cancer cells. Thus, catenin protein expression, regulation and localization are potentially important markers to predict motility and invasiveness of epithelial neoplasms. The regions of both alpha- and beta-catenin, located on 5q21-22 and 3p21, have been shown to be involved in the development of certain tumors (see Jiang, 1996; Schlosshauer et al., 2002) and reduced expressions of both alpha- and beta-catenin have been described in various tumors including breast carcinoma (Hashizume et al, 1996). Besides adhesion functions, beta-catenin binds to the tumor suppressor gene APC. APC mutation disturbs the equilibrium and cytoplasmic levels of free beta-catenin level in the cell and may have a role in tumorigenesis.

Immunogen :

Produced by immunization with recombinant human beta-catenin.

Specificity :

Recognizes an epitope in the carboxy terminus, amino acids 758-771, of human beta-catenin.

Package Size :

100 μg

Uses :

Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.