Integrin β1 Antibody, activated, clone HUTS-4, Azide Free | EMD Millipore MAB2079Z product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Integrin β1 Antibody, activated, clone HUTS-4, Azide Free

catalog :

MAB2079Z

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HUTS-4

The same clone is also sold as:

LifeSpan Biosciences: LS-B2861

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, proximity ligation assay, other

citations: 58

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:300; loading ...; fig 6a2 western blot; human; 1:1000; loading ...; fig 5a1	Shen C, Sun L, Zhu N, Qi F. Kindlin-1 contributes to EGF-induced re-epithelialization in skin wound healing. Int J Mol Med. 2017;39:949-959 pubmed publisher
immunohistochemistry; mouse; 1:100; loading ...; fig 5a	Chen Z, Givens C, Reader J, Tzima E. Haemodynamics Regulate Fibronectin Assembly via PECAM. Sci Rep. 2017;7:41223 pubmed publisher
immunocytochemistry; human; loading ...; fig 4e western blot; human; loading ...; fig 4c	SILVA P, Mendoza P, Rivas S, Diaz J, Moraga C, Quest A, et al. Hypoxia promotes Rab5 activation, leading to tumor cell migration, invasion and metastasis. Oncotarget. 2016;7:29548-62 pubmed publisher
immunohistochemistry; human; 1:100; loading ...; fig s6a	Nader G, Ezratty E, Gundersen G. FAK, talin and PIPKI? regulate endocytosed integrin activation to polarize focal adhesion assembly. Nat Cell Biol. 2016;18:491-503 pubmed publisher
proximity ligation assay; human; 1:1000; fig s3	Fiore V, Strane P, Bryksin A, White E, Hagood J, Barker T. Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction. J Cell Biol. 2015;211:173-90 pubmed publisher
flow cytometry; human western blot; human	Pasqualon T, Pruessmeyer J, Weidenfeld S, Babendreyer A, Groth E, Schumacher J, et al. A transmembrane C-terminal fragment of syndecan-1 is generated by the metalloproteinase ADAM17 and promotes lung epithelial tumor cell migration and lung metastasis formation. Cell Mol Life Sci. 2015;72:3783-801 pubmed publisher
other; mouse; 1:250; loading ...; fig 2a	Woo J, Zhao X, Khan H, Penn C, Wang X, Joly Amado A, et al. Slingshot-Cofilin activation mediates mitochondrial and synaptic dysfunction via AÎ² ligation to Î²1-integrin conformers. Cell Death Differ. 2015;22:921-34 pubmed publisher
flow cytometry; human; fig 5	Sugahara K, Braun G, de Mendoza T, Kotamraju V, French R, Lowy A, et al. Tumor-penetrating iRGD peptide inhibits metastasis. Mol Cancer Ther. 2015;14:120-8 pubmed publisher
western blot; mouse	De Rossi G, Evans A, Kay E, Woodfin A, McKay T, Nourshargh S, et al. Shed syndecan-2 inhibits angiogenesis. J Cell Sci. 2014;127:4788-99 pubmed publisher
immunoprecipitation; human immunocytochemistry; human western blot; human	Bi J, Wang R, Zhang Y, Han X, Ampah K, Liu W, et al. Identification of nucleolin as a lipid-raft-dependent ?1-integrin-interacting protein in A375 cell migration. Mol Cells. 2013;36:507-17 pubmed publisher
flow cytometry; human immunocytochemistry; human; 1:300	Fu X, Xu M, Liu J, Qi Y, Li S, Wang H. Regulation of migratory activity of human keratinocytes by topography of multiscale collagen-containing nanofibrous matrices. Biomaterials. 2014;35:1496-506 pubmed publisher
immunocytochemistry; human western blot; human; fig 2	Khotskaya Y, Beck B, Hurst D, Han Z, Xia W, Hung M, et al. Expression of metastasis suppressor BRMS1 in breast cancer cells results in a marked delay in cellular adhesion to matrix. Mol Carcinog. 2014;53:1011-26 pubmed publisher
western blot; human	Abbonante V, Gruppi C, Rubel D, Gross O, Moratti R, Balduini A. Discoidin domain receptor 1 protein is a novel modulator of megakaryocyte-collagen interactions. J Biol Chem. 2013;288:16738-46 pubmed publisher
	Aberg M, Eden D, Siegbahn A. Activation of β1 integrins and caveolin-1 by TF/FVIIa promotes IGF-1R signaling and cell survival. Apoptosis. 2020;25:519-534 pubmed publisher
	Kalappurakkal J, Anilkumar A, Patra C, van Zanten T, Sheetz M, Mayor S. Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading. Cell. 2019;: pubmed publisher
	Meshik X, O Neill P, Gautam N. Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration. ACS Synth Biol. 2019;8:498-510 pubmed publisher
	Zhang H, Fredericks T, Xiong G, Qi Y, Rychahou P, Li J, et al. Membrane associated collagen XIII promotes cancer metastasis and enhances anoikis resistance. Breast Cancer Res. 2018;20:116 pubmed publisher
	Spiess M, Hernández Varas P, Oddone A, Olofsson H, Blom H, Waithe D, et al. Active and inactive β1 integrins segregate into distinct nanoclusters in focal adhesions. J Cell Biol. 2018;217:1929-1940 pubmed publisher
	Guo L, Cai T, Chen K, Wang R, Wang J, Cui C, et al. Kindlin-2 regulates mesenchymal stem cell differentiation through control of YAP1/TAZ. J Cell Biol. 2018;217:1431-1451 pubmed publisher
	Huet Calderwood C, Rivera Molina F, Iwamoto D, Kromann E, Toomre D, Calderwood D. Novel ecto-tagged integrins reveal their trafficking in live cells. Nat Commun. 2017;8:570 pubmed publisher
	Wan Q, TruongVo T, Steele H, Ozcelikkale A, Han B, Wang Y, et al. Subcellular domain-dependent molecular hierarchy of SFK and FAK in mechanotransduction and cytokine signaling. Sci Rep. 2017;7:9033 pubmed publisher
	Wu B, Wang Y, Yang X, Xu B, Feng F, Wang B, et al. Basigin-mediated redistribution of CD98 promotes cell spreading and tumorigenicity in hepatocellular carcinoma. J Exp Clin Cancer Res. 2015;34:110 pubmed publisher
	Li L, Jiang H, Xie Y, Liao L, Cao H, Xu X, et al. Macrolide analog F806 suppresses esophageal squamous cell carcinoma (ESCC) by blocking β1 integrin activation. Oncotarget. 2015;6:15940-52 pubmed
	Pan L, North H, Sahni V, Jeong S, McGuire T, Berns E, et al. ?1-Integrin and integrin linked kinase regulate astrocytic differentiation of neural stem cells. PLoS ONE. 2014;9:e104335 pubmed publisher
	Lee S, Cheng H, Yuan Y, Wu S. Regulation of ionizing radiation-induced adhesion of breast cancer cells to fibronectin by alpha5beta1 integrin. Radiat Res. 2014;181:650-8 pubmed publisher
	Lee S, Kim J, Cho S, Kim H, Shin H, Jeon J, et al. TIMP-1 modulates chemotaxis of human neural stem cells through CD63 and integrin signalling. Biochem J. 2014;459:565-76 pubmed publisher
	Itokazu Y, Pagano R, Schroeder A, O Grady S, Limper A, Marks D. Reduced GM1 ganglioside in CFTR-deficient human airway cells results in decreased ?1-integrin signaling and delayed wound repair. Am J Physiol Cell Physiol. 2014;306:C819-30 pubmed publisher
	Lim H, Couchman J. Syndecan-2 regulation of morphology in breast carcinoma cells is dependent on RhoGTPases. Biochim Biophys Acta. 2014;1840:2482-90 pubmed publisher
	Cheng H, Lee S, Wu S. Effects of N-acetyl-L-cysteine on adhesive strength between breast cancer cell and extracellular matrix proteins after ionizing radiation. Life Sci. 2013;93:798-803 pubmed publisher
	Yang Y, Hong Q, Shi P, Liu Z, Luo J, Shao Z. Elevated expression of syntenin in breast cancer is correlated with lymph node metastasis and poor patient survival. Breast Cancer Res. 2013;15:R50 pubmed publisher
	Wang R, Bi J, Ampah K, Zhang C, Li Z, Jiao Y, et al. Lipid raft regulates the initial spreading of melanoma A375 cells by modulating ?1 integrin clustering. Int J Biochem Cell Biol. 2013;45:1679-89 pubmed publisher
	Wilk C, Schildberg F, Lauterbach M, Cadeddu R, Fröbel J, Westphal V, et al. The tissue inhibitor of metalloproteinases-1 improves migration and adhesion of hematopoietic stem and progenitor cells. Exp Hematol. 2013;41:823-831.e2 pubmed publisher
	Chiang Y, Ho K, Sun C, Chiu J, Lee F, Liao F, et al. CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell adhesion and migration. PLoS ONE. 2013;8:e61761 pubmed publisher
	Zimmerman C, Austin P, Khong A, McLeod S, Bean B, Forestieri R, et al. The small molecule genkwanine M induces single mode, mesenchymal tumor cell motility. Exp Cell Res. 2013;319:908-17 pubmed publisher
	Srikanth M, Das S, Berns E, Kim J, Stupp S, Kessler J. Nanofiber-mediated inhibition of focal adhesion kinase sensitizes glioma stemlike cells to epidermal growth factor receptor inhibition. Neuro Oncol. 2013;15:319-29 pubmed publisher
	Yao H, Duan M, Yang L, Buch S. Nonmuscle myosin light-chain kinase mediates microglial migration induced by HIV Tat: involvement of ?1 integrins. FASEB J. 2013;27:1532-48 pubmed publisher
	Babayan A, Kramar E, Barrett R, Jafari M, Haettig J, Chen L, et al. Integrin dynamics produce a delayed stage of long-term potentiation and memory consolidation. J Neurosci. 2012;32:12854-61 pubmed publisher
	Madera L, Hancock R. Synthetic immunomodulatory peptide IDR-1002 enhances monocyte migration and adhesion on fibronectin. J Innate Immun. 2012;4:553-68 pubmed publisher
	Xia H, Seeman J, Hong J, Hergert P, Bodem V, Jessurun J, et al. Low ?(2)?(1) integrin function enhances the proliferation of fibroblasts from patients with idiopathic pulmonary fibrosis by activation of the ?-catenin pathway. Am J Pathol. 2012;181:222-33 pubmed publisher
	Lippert D, Wilkins J. Glia maturation factor gamma regulates the migration and adherence of human T lymphocytes. BMC Immunol. 2012;13:21 pubmed
	Wang L, Pedroja B, Meyers E, Garcia A, Twining S, Bernstein A. Degradation of internalized ?v?5 integrin is controlled by uPAR bound uPA: effect on ?1 integrin activity and ?-SMA stress fiber assembly. PLoS ONE. 2012;7:e33915 pubmed publisher
	Lei W, Xing S, Deng C, Ju X, Jiang X, Luo Z. Laminin/?1 integrin signal triggers axon formation by promoting microtubule assembly and stabilization. Cell Res. 2012;22:954-72 pubmed publisher
	Wan S, Lin C, Lu Y, Lei H, Anderson R, Lin Y. Endothelial cell surface expression of protein disulfide isomerase activates ?1 and ?3 integrins and facilitates dengue virus infection. J Cell Biochem. 2012;113:1681-91 pubmed publisher
	Zou L, Cao S, Kang N, Huebert R, Shah V. Fibronectin induces endothelial cell migration through ?1 integrin and Src-dependent phosphorylation of fibroblast growth factor receptor-1 at tyrosines 653/654 and 766. J Biol Chem. 2012;287:7190-202 pubmed publisher
	Huang C, Fu X, Liu J, Qi Y, Li S, Wang H. The involvement of integrin ?1 signaling in the migration and myofibroblastic differentiation of skin fibroblasts on anisotropic collagen-containing nanofibers. Biomaterials. 2012;33:1791-800 pubmed publisher
	van de Vijver E, De Cuyper I, Gerrits A, Verhoeven A, Seeger K, Gutierrez L, et al. Defects in Glanzmann thrombasthenia and LAD-III (LAD-1/v) syndrome: the role of integrin ?1 and ?3 in platelet adhesion to collagen. Blood. 2012;119:583-6 pubmed publisher
	Das M, Ithychanda S, Qin J, Plow E. Migfilin and filamin as regulators of integrin activation in endothelial cells and neutrophils. PLoS ONE. 2011;6:e26355 pubmed publisher
	Augoff K, Das M, Bialkowska K, McCue B, Plow E, Sossey Alaoui K. miR-31 is a broad regulator of ?1-integrin expression and function in cancer cells. Mol Cancer Res. 2011;9:1500-8 pubmed publisher
	Kigel B, Rabinowicz N, Varshavsky A, Kessler O, Neufeld G. Plexin-A4 promotes tumor progression and tumor angiogenesis by enhancement of VEGF and bFGF signaling. Blood. 2011;118:4285-96 pubmed publisher
	Quast T, Eppler F, Semmling V, Schild C, Homsi Y, Levy S, et al. CD81 is essential for the formation of membrane protrusions and regulates Rac1-activation in adhesion-dependent immune cell migration. Blood. 2011;118:1818-27 pubmed publisher
	Jan H, Wei M, Peng C, Lin S, Lai P, Shieh M. The use of polyethylenimine-DNA to topically deliver hTERT to promote hair growth. Gene Ther. 2012;19:86-93 pubmed publisher
	Liu X, Yan Z, Huang L, Guo M, Zhang Z, Guo C. Cell surface heat shock protein 90 modulates prostate cancer cell adhesion and invasion through the integrin-?1/focal adhesion kinase/c-Src signaling pathway. Oncol Rep. 2011;25:1343-51 pubmed publisher
	Ohsawa K, Irino Y, Sanagi T, Nakamura Y, Suzuki E, Inoue K, et al. P2Y12 receptor-mediated integrin-beta1 activation regulates microglial process extension induced by ATP. Glia. 2010;58:790-801 pubmed publisher
	Matsuda Y, Schlange T, Oakeley E, Boulay A, Hynes N. WNT signaling enhances breast cancer cell motility and blockade of the WNT pathway by sFRP1 suppresses MDA-MB-231 xenograft growth. Breast Cancer Res. 2009;11:R32 pubmed publisher
	Chigaev A, Waller A, Amit O, Halip L, Bologa C, Sklar L. Real-time analysis of conformation-sensitive antibody binding provides new insights into integrin conformational regulation. J Biol Chem. 2009;284:14337-46 pubmed publisher
	Ithychanda S, Das M, Ma Y, Ding K, Wang X, Gupta S, et al. Migfilin, a molecular switch in regulation of integrin activation. J Biol Chem. 2009;284:4713-22 pubmed publisher
	Singh R, Holicky E, Cheng Z, Kim S, Wheatley C, Marks D, et al. Inhibition of caveolar uptake, SV40 infection, and beta1-integrin signaling by a nonnatural glycosphingolipid stereoisomer. J Cell Biol. 2007;176:895-901 pubmed
	Jung K, Liu X, Chirco R, Fridman R, Kim H. Identification of CD63 as a tissue inhibitor of metalloproteinase-1 interacting cell surface protein. EMBO J. 2006;25:3934-42 pubmed

product information

Catalog Number :

MAB2079Z

Subcategory :

Cell Structure

Product Name :

Anti-Integrin β1 Antibody, activated, clone HUTS-4, Azide Free

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P05556

Host Name :

Mouse

Antigen :

Integrin β1

Clone :

HUTS-4

Conjugate :

Purified

Isotype :

IgG2b

Product Description :

Anti-Integrin β1 Antibody, activated, clone HUTS-4, Azide Free

Cross Reactivity :

Human;Rat

Background :

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

ALT Names :

Ligand-inducedBindingSite;LIBS;CLIBS;MAB2079

Immunogen :

Generated against human beta1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues. MAB2079Z was specifically selected due to its increased reactivity with T-lymphoblasts upon Mn2+ stimulation.

Specificity :

Monoclonal antibody MAB2079Z is specific for active conformation of human beta1 integrins. The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit nonconvalently associated with the common beta1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg2+ or Mn2+, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies such as MAB2079Z which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. Antibody MAB2079Z recognizes epitopes in the 355-425 region of the common beta1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli.

Package Size :

100 μg

Uses :

ELISA;Flow Cytometry;Immunohistochemistry;Western Blotting

Storage :

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.