Integrin αVβ6 Antibody, clone 10D5, azide free | EMD Millipore MAB2077Z product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Integrin αVβ6 Antibody, clone 10D5, azide free

catalog :

MAB2077Z

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10D5

The same clone is also sold as:

Abcam: ab77906

reactivity :

human, bovine

application :

western blot, immunoprecipitation, flow cytometry, blocking or activating experiments

citations: 29

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig s2b	Kim D, Lee S, Fu Y, Jing F, Kim W, Hong S, et al. Del-1, an Endogenous Inhibitor of TGF-β Activation, Attenuates Fibrosis. Front Immunol. 2020;11:68 pubmed publisher
flow cytometry; bovine	Guzman E, Taylor G, Hope J, Herbert R, Cubillos Zapata C, Charleston B. Transduction of skin-migrating dendritic cells by human adenovirus 5 occurs via an actin-dependent phagocytic pathway. J Gen Virol. 2016;97:2703-2718 pubmed publisher
western blot; human; fig 2	Shimmon G, Wood B, Morris A, Mioulet V, Grazioli S, Brocchi E, et al. Truncated Bovine Integrin Alpha-v/Beta-6 as a Universal Capture Ligand for FMD Diagnosis. PLoS ONE. 2016;11:e0160696 pubmed publisher
flow cytometry; human; loading ...; fig 7b	Lu H, Wang T, Li J, Fedele C, Liu Q, Zhang J, et al. αvβ6 Integrin Promotes Castrate-Resistant Prostate Cancer through JNK1-Mediated Activation of Androgen Receptor. Cancer Res. 2016;76:5163-74 pubmed publisher
blocking or activating experiments; human; loading ...; fig 4b	Grudzien Nogalska E, Jiao X, Song M, Hart R, Kiledjian M. Nudt3 is an mRNA decapping enzyme that modulates cell migration. RNA. 2016;22:773-81 pubmed publisher
blocking or activating experiments; human	Wei Y, Zhang Y, Cai H, Mirza A, Iorio R, Peeples M, et al. Roles of the putative integrin-binding motif of the human metapneumovirus fusion (f) protein in cell-cell fusion, viral infectivity, and pathogenesis. J Virol. 2014;88:4338-52 pubmed publisher
flow cytometry; human; 10 ug/ml	Man Y, DiCara D, Chan N, Vessillier S, Mather S, Rowe M, et al. Structural guided scaffold phage display libraries as a source of bio-therapeutics. PLoS ONE. 2013;8:e70452 pubmed publisher
	Merilahti P, Tauriainen S, Susi P. Human Parechovirus 1 Infection Occurs via αVβ1 Integrin. PLoS ONE. 2016;11:e0154769 pubmed publisher
	Li X, Liu Y, Haas T. Peptides derived from central turn motifs within integrin Î±IIb and Î±V cytoplasmic tails inhibit integrin activation. Peptides. 2014;62:38-48 pubmed publisher
	John A, Luckett J, Tatler A, Awais R, Desai A, Habgood A, et al. Preclinical SPECT/CT imaging of ?v?6 integrins for molecular stratification of idiopathic pulmonary fibrosis. J Nucl Med. 2013;54:2146-52 pubmed publisher
	Allen M, Thomas G, Clark S, Dawoud M, Vallath S, Payne S, et al. Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of ?v?6 integrin in DCIS identifies high-risk patients and predicts recurrence. Clin Cancer Res. 2014;20:344-57 pubmed publisher
	Jones A, Lambert D, Speight P, Whawell S. ADAM 10 is over expressed in oral squamous cell carcinoma and contributes to invasive behaviour through a functional association with ?v?6 integrin. FEBS Lett. 2013;587:3529-34 pubmed publisher
	Eberlein C, Kendrew J, McDaid K, Alfred A, Kang J, Jacobs V, et al. A human monoclonal antibody 264RAD targeting ?v?6 integrin reduces tumour growth and metastasis, and modulates key biomarkers in vivo. Oncogene. 2013;32:4406-16 pubmed publisher
	Canonici A, Pellegrino E, Siret C, Terciolo C, Czerucka D, Bastonero S, et al. Saccharomyces boulardii improves intestinal epithelial cell restitution by inhibiting ?v?5 integrin activation state. PLoS ONE. 2012;7:e45047 pubmed publisher
	Brown A, Fiore V, Sulchek T, Barker T. Physical and chemical microenvironmental cues orthogonally control the degree and duration of fibrosis-associated epithelial-to-mesenchymal transitions. J Pathol. 2013;229:25-35 pubmed publisher
	Siu A, Lee C, Pham E, Ramos D. Revisiting epithelial-to-mesenchymal transition through adenoid cystic carcinoma. Anticancer Res. 2012;32:3683-8 pubmed
	Frith J, Mills R, Hudson J, Cooper White J. Tailored integrin-extracellular matrix interactions to direct human mesenchymal stem cell differentiation. Stem Cells Dev. 2012;21:2442-56 pubmed publisher
	Curnis F, Gasparri A, Longhi R, Colombo B, D Alessio S, Pastorino F, et al. Chromogranin A binds to ?v?6-integrin and promotes wound healing in mice. Cell Mol Life Sci. 2012;69:2791-803 pubmed publisher
	Goswami R, Bajjuri K, Forsyth J, Das S, Hassenpflug W, Huang Z, et al. Chemically programmed antibodies targeting multiple alpha(v) integrins and their effects on tumor-related functions in vitro. Bioconjug Chem. 2011;22:1535-44 pubmed publisher
	Grzesiak J, Tran Cao H, Burton D, Kaushal S, Vargas F, Clopton P, et al. Knockdown of the ?(1) integrin subunit reduces primary tumor growth and inhibits pancreatic cancer metastasis. Int J Cancer. 2011;129:2905-15 pubmed publisher
	Shimamura M, Murphy Ullrich J, Britt W. Human cytomegalovirus induces TGF-?1 activation in renal tubular epithelial cells after epithelial-to-mesenchymal transition. PLoS Pathog. 2010;6:e1001170 pubmed publisher
	Alexopoulou A, Leao M, Caballero O, Da Silva L, Reid L, Lakhani S, et al. Dissecting the transcriptional networks underlying breast cancer: NR4A1 reduces the migration of normal and breast cancer cell lines. Breast Cancer Res. 2010;12:R51 pubmed publisher
	Heikkilä O, Susi P, Tevaluoto T, Härmä H, Marjomaki V, Hyypia T, et al. Internalization of coxsackievirus A9 is mediated by {beta}2-microglobulin, dynamin, and Arf6 but not by caveolin-1 or clathrin. J Virol. 2010;84:3666-81 pubmed publisher
	Heikkilä O, Susi P, Stanway G, Hyypia T. Integrin alphaVbeta6 is a high-affinity receptor for coxsackievirus A9. J Gen Virol. 2009;90:197-204 pubmed publisher
	Bruun C, Jørgensen C, Bay L, Cirera S, Jensen H, Leifsson P, et al. Phenotypic and genetic characterization of a novel phenotype in pigs characterized by juvenile hairlessness and age dependent emphysema. BMC Genomics. 2008;9:283 pubmed publisher
	Fujiwara T, Kawakatsu T, Tayama S, Kobayashi Y, Sugiura N, Kimata K, et al. Hyaluronan-CD44 pathway regulates orientation of mitotic spindle in normal epithelial cells. Genes Cells. 2008;13:759-70 pubmed publisher
	Elayadi A, Samli K, Prudkin L, Liu Y, Bian A, Xie X, et al. A peptide selected by biopanning identifies the integrin alphavbeta6 as a prognostic biomarker for nonsmall cell lung cancer. Cancer Res. 2007;67:5889-95 pubmed
	Fouchier F, Penel C, Pierre Montero M, Bremond P, Champion S. Integrin alphavbeta6 mediates HT29-D4 cell adhesion to MMP-processed fibrinogen in the presence of Mn2+. Eur J Cell Biol. 2007;86:143-60 pubmed
	Pameijer C, Navanjo A, Meechoovet B, Wagner J, Aguilar B, Wright C, et al. Conversion of a tumor-binding peptide identified by phage display to a functional chimeric T cell antigen receptor. Cancer Gene Ther. 2007;14:91-7 pubmed

product information

Catalog Number :

MAB2077Z

Subcategory :

Cell Structure

Product Name :

Anti-Integrin αVβ6 Antibody, clone 10D5, azide free

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P06756

Host Name :

Mouse

Antigen :

Integrin αVβ6

Clone :

10D5

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Integrin αVβ6 Antibody, clone 10D5, azide free

Cross Reactivity :

Human;Mouse;Rat

Background :

The integrin family of cell adhesion receptors consists of at least 19 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. To date, 16 different alpha-subunits and 8 different beta-subunits have been identified. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Integrin alphaV is a type I membrane glycoptroein with no I (inserted) domain. The alphaV protein is post-translationally cleaved into a heavy and light chain, which is expressed on the cell surface as a disulfide linked polypeptide. The integrin alphavbeta6 is expressed on a variety of epithelial cells during dynamic processes including organogenesis, tissue injury and malignant transformation.

ALT Names :

MAB2077

Immunogen :

Immunization of beta 6 knockout mice with wild type murine keratinocytes.

Specificity :

Monoclonal antibody MAB2077Z, raised by immunizing beta6 knockout mice with wild type murine keratinocytes, recognizes human and murine alphaVbeta6 by flow cytometry and immunoprecipitation and has blocking activity against murine and human alphaVbeta6. 100% inhibition of alphaVbeta6-mediated cell adhesion and cell migration are achieved at a concentration of 10 μg/mL. Because the antibody was raised in knockout mice, it is likely to recognize the receptor in other species, however it has been evaluated only in human and mouse.The antibody is specific for alphaVbeta6 and does not recognize aV or other aV integrins.

Package Size :

100 μg

Uses :

Flow Cytometry;Immunoprecipitation;Affects Function

Storage :

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.