Mucin MUC5AC Antibody, clone CLH2 | EMD Millipore MAB2011 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Mucin MUC5AC Antibody, clone CLH2

catalog :

MAB2011

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CLH2

The same clone is also sold as:

Novus Biologicals: NBP2-44455-0.1mg
Abnova: MAB13374, MAB13373
LifeSpan Biosciences: LS-C312111, LS-C152909, LS-C348684, LS-B2434
Santa Cruz Biotechnology: sc-33667

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry knockout validation

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry knockout validation; human; 1:500; loading ...; fig 6d	Pothuraju R, Rachagani S, Krishn S, Chaudhary S, Nimmakayala R, Siddiqui J, et al. Molecular implications of MUC5AC-CD44 axis in colorectal cancer progression and chemoresistance. Mol Cancer. 2020;19:37 pubmed publisher
immunohistochemistry; human; fig s2b	Arthur G, Duffy S, Roach K, Hirst R, Shikotra A, Gaillard E, et al. KCa3.1 K+ Channel Expression and Function in Human Bronchial Epithelial Cells. PLoS ONE. 2015;10:e0145259 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig 3,4a	Du R, Richmond B, Cates J, Massion P, Ware L, Lee J, et al. Secretory IgA from submucosal glands does not compensate for its airway surface deficiency in chronic obstructive pulmonary disease. Virchows Arch. 2015;467:657-665 pubmed
immunocytochemistry; human	Bai J, Smock S, Jackson G, Macisaac K, Huang Y, Mankus C, et al. Phenotypic responses of differentiated asthmatic human airway epithelial cultures to rhinovirus. PLoS ONE. 2015;10:e0118286 pubmed publisher
	Chand H, Harris J, Tesfaigzi Y. IL-13 in LPS-Induced Inflammation Causes Bcl-2 Expression to Sustain Hyperplastic Mucous cells. Sci Rep. 2018;8:436 pubmed publisher
	Kang M, Lee K, Jang J, Kwon W, Park J, Chang Y, et al. Disease spectrum of intraductal papillary mucinous neoplasm with an associated invasive carcinoma invasive IPMN versus pancreatic ductal adenocarcinoma-associated IPMN. Pancreas. 2013;42:1267-74 pubmed
	Kang M, Lee K, Jang J, Han I, Kim S. Evaluation of clinical meaning of histological subtypes of intraductal papillary mucinous neoplasm of the pancreas. Pancreas. 2013;42:959-66 pubmed publisher
	Davies J, Wickström C, Thornton D. Gel-forming and cell-associated mucins: preparation for structural and functional studies. Methods Mol Biol. 2012;842:27-47 pubmed publisher
	Polosukhin V, Cates J, Lawson W, Milstone A, Matafonov A, Massion P, et al. Hypoxia-inducible factor-1 signalling promotes goblet cell hyperplasia in airway epithelium. J Pathol. 2011;224:203-11 pubmed publisher
	Mebratu Y, Schwalm K, Smith K, Schuyler M, Tesfaigzi Y. Cigarette smoke suppresses Bik to cause epithelial cell hyperplasia and mucous cell metaplasia. Am J Respir Crit Care Med. 2011;183:1531-8 pubmed publisher
	Wu Y, Nowack D, Omenn G, Haab B. Mucin glycosylation is altered by pro-inflammatory signaling in pancreatic-cancer cells. J Proteome Res. 2009;8:1876-86 pubmed publisher
	Karacsonyi C, Shanmugam N, Kagan E. A clinically relevant in vitro model for evaluating the effects of aerosolized vesicants. Toxicol Lett. 2009;185:38-44 pubmed publisher
	Smith R, Stern B, Smith A. Mucin immunohistochemistry in the diagnosis and mapping of extramammary Paget's disease. J Cell Mol Med. 2008;12:1605-10 pubmed

product information

Catalog Number :

MAB2011

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-Mucin MUC5AC Antibody, clone CLH2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q6W4X9

Host Name :

Mouse

Antigen :

Mucin MUC5AC

Clone :

CLH2

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Mucin MUC5AC Antibody, clone CLH2

Cross Reactivity :

Human

Background :

Mucins are high molecular weight glycoproteins that are heavily glycosylated with oligosaccharide side chains linked O-glycosidically to the protein backbone. At least nine mucin genes have been identified to date. The pattern of tissue and cell-specific expression of these mucin genes suggests a distinct role for each member of the diverse mucin family. In epithelial cancers, many of the phenotypic markers for pre-malignant and malignant cells have been found on the carbohydrate and peptide moieties of mucin glycoproteins. Deregulation of tissue and cell-specific expression of mucin genes has also been described in epithelial cancers. At present, the role of mucin glyoproteins in various states of epithelial cell carcinogenesis, including the pre-neoplastic state and metastasis, is under investigation.

ALT Names :

Gastric Mucin

Immunogen :

Directed against a synthetic peptide of the MUC5AC tandem repeat.

Specificity :

Antibody MAB2011 is specific for the MUC5AC tandem repeat of human mucin. No cross reactivity with other peptides from MUC2, 3, 6, or 7. Immunoreactivity of MAB2011 has been detected in the following normal human epithelial tissues: bronchus, endocervix, stomach, and gallbladder. The predominant staining pattern in normal tissues is perinuclear. Antibody reactivity has been observed in foveolar and mucopeptic neck cells of normal gastric mucosa. The expression of MUC5AC has been frequently observed in gastric carcinomas and may be used as a marker of gastric differentiation. The CLH2 antibody shows a weak reactivity with a high molecular weight smear characteristic of mucin from native mucin extract from normal gastric mucosa and from gastric diffuse carcinoma . The antibody was generated by immunizing mice with a synthetic tandem repeat peptide The antibody recognizes the sequence TTSTTSAP within the tandem repeat of MUC5AC (1). It further recognizes glycosylated as well as unglycosylated MUC5AC.

Package Size :

100 µg

Uses :

ELISA;Immunoprecipitation;Western Blotting;Immunocytochemistry;Immunohistochemistry (Paraffin)

Storage :

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.