This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Integrin α5β1 Antibody, clone HA5
catalog :
MAB1999
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HA5
reactivity :
Chinese hamsters, human, mouse
application :
immunocytochemistry, immunoprecipitation, flow cytometry
citations: 23
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig s6b
Owen L, Adhikari A, Patel M, Grimmer P, Leijnse N, Kim M, et al. A cytoskeletal clutch mediates cellular force transmission in a soft, three-dimensional extracellular matrix. Mol Biol Cell. 2017;28:1959-1974 pubmed publisher
  • flow cytometry; Chinese hamsters; fig 1
Hang Q, Isaji T, Hou S, Zhou Y, Fukuda T, Gu J. N-Glycosylation of integrin ?5 acts as a switch for EGFR-mediated complex formation of integrin ?5?1 to ?6?4. Sci Rep. 2016;6:33507 pubmed publisher
  • flow cytometry; human; fig 5d
Vergani E, Di Guardo L, Dugo M, Rigoletto S, Tragni G, Ruggeri R, et al. Overcoming melanoma resistance to vemurafenib by targeting CCL2-induced miR-34a, miR-100 and miR-125b. Oncotarget. 2016;7:4428-41 pubmed publisher
  • flow cytometry; human
Pelillo C, Bergamo A, Mollica H, Bestagno M, Sava G. Colorectal Cancer Metastases Settle in the Hepatic Microenvironment Through α5β1 Integrin. J Cell Biochem. 2015;116:2385-96 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7B
Farnoodian M, Kinter J, Yadranji Aghdam S, Zaitoun I, Sorenson C, Sheibani N. Expression of pigment epithelium-derived factor and thrombospondin-1 regulate proliferation and migration of retinal pigment epithelial cells. Physiol Rep. 2015;3: pubmed publisher
  • flow cytometry; mouse
Shin E, Huang Q, Gurel Z, Sorenson C, Sheibani N. High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress. PLoS ONE. 2014;9:e103148 pubmed publisher
  • flow cytometry; human
Sallach J, Di Pasquale G, Larcher F, Niehoff N, Rübsam M, Huber A, et al. Tropism-modified AAV vectors overcome barriers to successful cutaneous therapy. Mol Ther. 2014;22:929-39 pubmed publisher
  • flow cytometry; mouse; 1:200
Zhao Y, Wang S, Sorenson C, Teixeira L, Dubielzig R, Peters D, et al. Cyp1b1 mediates periostin regulation of trabecular meshwork development by suppression of oxidative stress. Mol Cell Biol. 2013;33:4225-40 pubmed publisher
  • flow cytometry; human; 1:100
Caffarel M, Chattopadhyay A, Araujo A, Bauer J, Scarpini C, Coleman N. Tissue transglutaminase mediates the pro-malignant effects of oncostatin M receptor over-expression in cervical squamous cell carcinoma. J Pathol. 2013;231:168-79 pubmed publisher
Merilahti P, Tauriainen S, Susi P. Human Parechovirus 1 Infection Occurs via αVβ1 Integrin. PLoS ONE. 2016;11:e0154769 pubmed publisher
Haubner R, Maschauer S, Einsiedel J, Eder I, Rangger C, Gmeiner P, et al. H-CRRETAWAC-OH, a lead structure for the development of radiotracer targeting integrin α5β1?. Biomed Res Int. 2014;2014:243185 pubmed publisher
Lee S, Cheng H, Yuan Y, Wu S. Regulation of ionizing radiation-induced adhesion of breast cancer cells to fibronectin by alpha5beta1 integrin. Radiat Res. 2014;181:650-8 pubmed publisher
Serres E, Debarbieux F, Stanchi F, Maggiorella L, Grall D, Turchi L, et al. Fibronectin expression in glioblastomas promotes cell cohesion, collective invasion of basement membrane in vitro and orthotopic tumor growth in mice. Oncogene. 2014;33:3451-62 pubmed publisher
Morozevich G, Kozlova N, Ushakova N, Preobrazhenskaya M, Berman A. Integrin ?5?1 simultaneously controls EGFR-dependent proliferation and Akt-dependent pro-survival signaling in epidermoid carcinoma cells. Aging (Albany NY). 2012;4:368-74 pubmed
Kesanakurti D, Chetty C, Dinh D, Gujrati M, Rao J. Role of MMP-2 in the regulation of IL-6/Stat3 survival signaling via interaction with ?5?1 integrin in glioma. Oncogene. 2013;32:327-40 pubmed publisher
Day P, Riggs K, Hasan N, Corbin D, Humphrey D, Hu C. Syntaxins 3 and 4 mediate vesicular trafficking of ?5?1 and ?3?1 integrins and cancer cell migration. Int J Oncol. 2011;39:863-71 pubmed publisher
Nevo J, Mai A, Tuomi S, Pellinen T, Pentikäinen O, Heikkila P, et al. Mammary-derived growth inhibitor (MDGI) interacts with integrin ?-subunits and suppresses integrin activity and invasion. Oncogene. 2010;29:6452-63 pubmed publisher
Tall E, Bernstein A, Oliver N, Gray J, Masur S. TGF-?-stimulated CTGF production enhanced by collagen and associated with biogenesis of a novel 31-kDa CTGF form in human corneal fibroblasts. Invest Ophthalmol Vis Sci. 2010;51:5002-11 pubmed publisher
Kim H, Kim P, Bae S, Son H, Thoudam D, Kim J, et al. Transforming growth factor-beta-induced protein (TGFBIp/beta ig-h3) activates platelets and promotes thrombogenesis. Blood. 2009;114:5206-15 pubmed publisher
Garcia A, Dellatore S, Messersmith P, Miller W. Effects of supported lipid monolayer fluidity on the adhesion of hematopoietic progenitor cell lines to fibronectin-derived peptide ligands for alpha5beta1 and alpha4beta1 integrins. Langmuir. 2009;25:2994-3002 pubmed
Sato Y, Isaji T, Tajiri M, Yoshida Yamamoto S, Yoshinaka T, Somehara T, et al. An N-glycosylation site on the beta-propeller domain of the integrin alpha5 subunit plays key roles in both its function and site-specific modification by beta1,4-N-acetylglucosaminyltransferase III. J Biol Chem. 2009;284:11873-81 pubmed publisher
Isaji T, Sato Y, Zhao Y, Miyoshi E, Wada Y, Taniguchi N, et al. N-glycosylation of the beta-propeller domain of the integrin alpha5 subunit is essential for alpha5beta1 heterodimerization, expression on the cell surface, and its biological function. J Biol Chem. 2006;281:33258-67 pubmed
Taliana L, Benezra M, Greenberg R, Masur S, Bernstein A. ZO-1: lamellipodial localization in a corneal fibroblast wound model. Invest Ophthalmol Vis Sci. 2005;46:96-103 pubmed
product information
Catalog Number :
MAB1999
Subcategory :
Cell Structure
Product Name :
Anti-Integrin α5β1 Antibody, clone HA5
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P08648
Host Name :
Mouse
Antigen :
Integrin α5β1
Clone :
HA5
Conjugate :
Purified
Isotype :
IgG2bκ
Product Description :
Anti-Integrin α5β1 Antibody, clone HA5
Cross Reactivity :
Human;Pig
ALT Names :
VLA-5
Specificity :
Reacts with the fibronectin receptor VLA-5 (alpha-5beta-1) integrin complex.
Package Size :
100 μg
Uses :
Flow Cytometry;Immunoprecipitation
Storage :
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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