This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Integrin β1 Antibody, clone P4C10, azide free
catalog :
MAB1987Z
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
P4C10

The same clone is also sold as:
reactivity :
human, mouse
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments
citations: 56
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; fig 3b
  • western blot; human; fig 3c
Suginami K, Sato Y, Horie A, Matsumoto H, Kyo S, Araki Y, et al. Platelets are a possible regulator of human endometrial re-epithelialization during menstruation. Am J Reprod Immunol. 2017;77: pubmed publisher
  • blocking or activating experiments; human; loading ...; fig s1c
Kolanowski S, van Schijndel G, Van Ham S, ten Brinke A. Adaptation to replating of dendritic cells synergizes with Toll-like receptor stimuli and enhances the pro-inflammatory cytokine profile. Cytotherapy. 2016;18:902-10 pubmed publisher
  • blocking or activating experiments; human; 1:200
Fu X, Xu M, Liu J, Qi Y, Li S, Wang H. Regulation of migratory activity of human keratinocytes by topography of multiscale collagen-containing nanofibrous matrices. Biomaterials. 2014;35:1496-506 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:60
Mortillo S, Elste A, Ge Y, Patil S, Hsiao K, Huntley G, et al. Compensatory redistribution of neuroligins and N-cadherin following deletion of synaptic ?1-integrin. J Comp Neurol. 2012;520:2041-52 pubmed publisher
  • immunocytochemistry; human; 10 ug/ml
Queisser M, Kouri F, Konigshoff M, Wygrecka M, Schubert U, Eickelberg O, et al. Loss of RAGE in pulmonary fibrosis: molecular relations to functional changes in pulmonary cell types. Am J Respir Cell Mol Biol. 2008;39:337-45 pubmed publisher
Meshik X, O Neill P, Gautam N. Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration. ACS Synth Biol. 2019;8:498-510 pubmed publisher
Haun F, Neumann S, Peintner L, Wieland K, Habicht J, Schwan C, et al. Identification of a novel anoikis signalling pathway using the fungal virulence factor gliotoxin. Nat Commun. 2018;9:3524 pubmed publisher
Guo L, Cai T, Chen K, Wang R, Wang J, Cui C, et al. Kindlin-2 regulates mesenchymal stem cell differentiation through control of YAP1/TAZ. J Cell Biol. 2018;217:1431-1451 pubmed publisher
Lamason R, Bastounis E, Kafai N, Serrano R, Del Alamo J, Theriot J, et al. Rickettsia Sca4 Reduces Vinculin-Mediated Intercellular Tension to Promote Spread. Cell. 2016;167:670-683.e10 pubmed publisher
Liu C, Hu R, Huang M, Lai I, Chen C, Lai H, et al. C1GALT1 promotes invasive phenotypes of hepatocellular carcinoma cells by modulating integrin ?1 glycosylation and activity. PLoS ONE. 2014;9:e94995 pubmed publisher
von Kietzell K, Pozzuto T, Heilbronn R, Grössl T, Fechner H, Weger S. Antibody-mediated enhancement of parvovirus B19 uptake into endothelial cells mediated by a receptor for complement factor C1q. J Virol. 2014;88:8102-15 pubmed publisher
Cheng N, van Zandwijk N, Reid G. Cilengitide inhibits attachment and invasion of malignant pleural mesothelioma cells through antagonism of integrins ?v?3 and ?v?5. PLoS ONE. 2014;9:e90374 pubmed publisher
Chen C, Wang S, Liu C, Wu Y, Wang W, Huang J, et al. ?-1,4-Galactosyltransferase III suppresses ?1 integrin-mediated invasive phenotypes and negatively correlates with metastasis in colorectal cancer. Carcinogenesis. 2014;35:1258-66 pubmed publisher
Trad A, Riese M, Shomali M, Hedeman N, Effenberger T, Grötzinger J, et al. The disintegrin domain of ADAM17 antagonises fibroblast?carcinoma cell interactions. Int J Oncol. 2013;42:1793-800 pubmed publisher
Jerke U, Rolle S, Purfürst B, Luft F, Nauseef W, Kettritz R. ?2 integrin-mediated cell-cell contact transfers active myeloperoxidase from neutrophils to endothelial cells. J Biol Chem. 2013;288:12910-9 pubmed publisher
Chang H, Chen C, Chou C, Liao Y, Huang M, Chen Y, et al. ?-1,4-Galactosyltransferase III enhances invasive phenotypes via ?1-integrin and predicts poor prognosis in neuroblastoma. Clin Cancer Res. 2013;19:1705-16 pubmed publisher
Orlando C, Ster J, Gerber U, Fawcett J, Raineteau O. Perisynaptic chondroitin sulfate proteoglycans restrict structural plasticity in an integrin-dependent manner. J Neurosci. 2012;32:18009-17, 18017a pubmed publisher
Wong W, Sigvardsson M, Astrand Grundström I, Hogge D, Larsson J, Qian H, et al. Expression of integrin ?2 receptor in human cord blood CD34+CD38-CD90+ stem cells engrafting long-term in NOD/SCID-IL2R?(c) null mice. Stem Cells. 2013;31:360-71 pubmed publisher
Liao W, Liu C, Huang M, Chen C, Hung J, Chou C, et al. Expression of GALNT2 in human extravillous trophoblasts and its suppressive role in trophoblast invasion. Placenta. 2012;33:1005-11 pubmed publisher
Isaac J, Tarapore P, Zhang X, Lam Y, Ho S. Site-specific S-nitrosylation of integrin ?6 increases the extent of prostate cancer cell migration by enhancing integrin ?1 association and weakening adherence to laminin-1. Biochemistry. 2012;51:9689-97 pubmed publisher
Babayan A, Kramar E, Barrett R, Jafari M, Haettig J, Chen L, et al. Integrin dynamics produce a delayed stage of long-term potentiation and memory consolidation. J Neurosci. 2012;32:12854-61 pubmed publisher
Teo G, Ankrum J, Martinelli R, Boetto S, Simms K, Sciuto T, et al. Mesenchymal stem cells transmigrate between and directly through tumor necrosis factor-?-activated endothelial cells via both leukocyte-like and novel mechanisms. Stem Cells. 2012;30:2472-86 pubmed publisher
Madera L, Hancock R. Synthetic immunomodulatory peptide IDR-1002 enhances monocyte migration and adhesion on fibronectin. J Innate Immun. 2012;4:553-68 pubmed publisher
Balzer E, Tong Z, Paul C, Hung W, Stroka K, Boggs A, et al. Physical confinement alters tumor cell adhesion and migration phenotypes. FASEB J. 2012;26:4045-56 pubmed publisher
Andrian E, Qi G, Wang J, Halperin S, Lee S. Role of surface proteins SspA and SspB of Streptococcus gordonii in innate immunity. Microbiology. 2012;158:2099-106 pubmed publisher
Beauséjour M, Noël D, Thibodeau S, Bouchard V, Harnois C, Beaulieu J, et al. Integrin/Fak/Src-mediated regulation of cell survival and anoikis in human intestinal epithelial crypt cells: selective engagement and roles of PI3-K isoform complexes. Apoptosis. 2012;17:566-78 pubmed publisher
Shields M, Krantz S, Bentrem D, Dangi Garimella S, Munshi H. Interplay between ?1-integrin and Rho signaling regulates differential scattering and motility of pancreatic cancer cells by snail and Slug proteins. J Biol Chem. 2012;287:6218-29 pubmed publisher
Lee C, Marzan D, Lin G, Goodison S, Silletti S. ?2 Integrin-Dependent Suppression of Pancreatic Adenocarcinoma Cell Invasion Involves Ectodomain Regulation of Kallikrein-Related Peptidase-5. J Oncol. 2011;2011:365651 pubmed publisher
Khaled S, Gupta K, Kucik D. Ionizing radiation increases adhesiveness of human aortic endothelial cells via a chemokine-dependent mechanism. Radiat Res. 2012;177:594-601 pubmed
Case L, Waterman C. Adhesive F-actin waves: a novel integrin-mediated adhesion complex coupled to ventral actin polymerization. PLoS ONE. 2011;6:e26631 pubmed publisher
Shyu M, Chen C, Lin N, Liao W, Chen C, Lin C, et al. MUC1 expression is elevated in severe preeclamptic placentas and suppresses trophoblast cell invasion via ?1-integrin signaling. J Clin Endocrinol Metab. 2011;96:3759-67 pubmed publisher
Guilluy C, Swaminathan V, Garcia Mata R, O Brien E, Superfine R, Burridge K. The Rho GEFs LARG and GEF-H1 regulate the mechanical response to force on integrins. Nat Cell Biol. 2011;13:722-7 pubmed publisher
Zhang Y, Chen M, Venugopal S, Zhou Y, Xiang W, Li Y, et al. Isthmin exerts pro-survival and death-promoting effect on endothelial cells through alphavbeta5 integrin depending on its physical state. Cell Death Dis. 2011;2:e153 pubmed publisher
Vartanian A, Stepanova E, Grigorieva I, Solomko E, Belkin V, Baryshnikov A, et al. Melanoma vasculogenic mimicry capillary-like structure formation depends on integrin and calcium signaling. Microcirculation. 2011;18:390-9 pubmed publisher
Baker A, Cox T, Bird D, Lang G, Murray G, Sun X, et al. The role of lysyl oxidase in SRC-dependent proliferation and metastasis of colorectal cancer. J Natl Cancer Inst. 2011;103:407-24 pubmed publisher
Nagaharu K, Zhang X, Yoshida T, Katoh D, Hanamura N, Kozuka Y, et al. Tenascin C induces epithelial-mesenchymal transition-like change accompanied by SRC activation and focal adhesion kinase phosphorylation in human breast cancer cells. Am J Pathol. 2011;178:754-63 pubmed publisher
Collie A, Bota P, Johns R, Maier R, Stayton P. Differential monocyte/macrophage interleukin-1? production due to biomaterial topography requires the ?2 integrin signaling pathway. J Biomed Mater Res A. 2011;96:162-9 pubmed publisher
Alexopoulou A, Leao M, Caballero O, Da Silva L, Reid L, Lakhani S, et al. Dissecting the transcriptional networks underlying breast cancer: NR4A1 reduces the migration of normal and breast cancer cell lines. Breast Cancer Res. 2010;12:R51 pubmed publisher
Liu W, Han Y, Liu Y, Song A, Barnes B, Song X. Spinal matrix metalloproteinase-9 contributes to physical dependence on morphine in mice. J Neurosci. 2010;30:7613-23 pubmed publisher
Maijenburg M, Noort W, Kleijer M, Kompier C, Weijer K, van Buul J, et al. Cell cycle and tissue of origin contribute to the migratory behaviour of human fetal and adult mesenchymal stromal cells. Br J Haematol. 2010;148:428-40 pubmed publisher
Zeituni A, Jotwani R, CARRION J, Cutler C. Targeting of DC-SIGN on human dendritic cells by minor fimbriated Porphyromonas gingivalis strains elicits a distinct effector T cell response. J Immunol. 2009;183:5694-704 pubmed publisher
Elphick G, Sarangi P, Hyun Y, Hollenbaugh J, Ayala A, Biffl W, et al. Recombinant human activated protein C inhibits integrin-mediated neutrophil migration. Blood. 2009;113:4078-85 pubmed publisher
van Gils J, Stutterheim J, van Duijn T, Zwaginga J, Porcelijn L, de Haas M, et al. HPA-1a alloantibodies reduce endothelial cell spreading and monolayer integrity. Mol Immunol. 2009;46:406-15 pubmed publisher
Schmid M, Varner J. Chapter 15. Methods to study myeloid cell roles in angiogenesis. Methods Enzymol. 2008;445:343-71 pubmed publisher
Mosig S, Rennert K, Krause S, Kzhyshkowska J, Neunübel K, Heller R, et al. Different functions of monocyte subsets in familial hypercholesterolemia: potential function of CD14+ CD16+ monocytes in detoxification of oxidized LDL. FASEB J. 2009;23:866-74 pubmed publisher
Chen Y, Zhang J, Wang Y, Zhang L, Julien R, Tang K, et al. Real-time monitoring approach: assessment of effects of antibodies on the adhesion of NCI-H460 cancer cells to the extracellular matrix. Biosens Bioelectron. 2008;23:1390-6 pubmed publisher
Yamamoto H, Nishi N, Shoji H, Itoh A, Lu L, Hirashima M, et al. Induction of cell adhesion by galectin-8 and its target molecules in Jurkat T-cells. J Biochem. 2008;143:311-24 pubmed
Smith M, Bivins Smith E, Tilley A, Bentz G, Chan G, Minard J, et al. Roles of phosphatidylinositol 3-kinase and NF-kappaB in human cytomegalovirus-mediated monocyte diapedesis and adhesion: strategy for viral persistence. J Virol. 2007;81:7683-94 pubmed
Kuo J, Wang W, Yao C, Wu P, Chen R. The tumor suppressor DAPK inhibits cell motility by blocking the integrin-mediated polarity pathway. J Cell Biol. 2006;172:619-31 pubmed
Shi Y, Ethell I. Integrins control dendritic spine plasticity in hippocampal neurons through NMDA receptor and Ca2+/calmodulin-dependent protein kinase II-mediated actin reorganization. J Neurosci. 2006;26:1813-22 pubmed
Goel H, Moro L, King M, Teider N, Centrella M, McCarthy T, et al. Beta1 integrins modulate cell adhesion by regulating insulin-like growth factor-II levels in the microenvironment. Cancer Res. 2006;66:331-42 pubmed
Bernard Trifilo J, Kramar E, Torp R, Lin C, Pineda E, Lynch G, et al. Integrin signaling cascades are operational in adult hippocampal synapses and modulate NMDA receptor physiology. J Neurochem. 2005;93:834-49 pubmed
Short S, Derrien A, Narsimhan R, Lawler J, Ingber D, Zetter B. Inhibition of endothelial cell migration by thrombospondin-1 type-1 repeats is mediated by beta1 integrins. J Cell Biol. 2005;168:643-53 pubmed
Goel H, Fornaro M, Moro L, Teider N, Rhim J, King M, et al. Selective modulation of type 1 insulin-like growth factor receptor signaling and functions by beta1 integrins. J Cell Biol. 2004;166:407-18 pubmed
Hintermann E, Bilban M, Sharabi A, Quaranta V. Inhibitory role of alpha 6 beta 4-associated erbB-2 and phosphoinositide 3-kinase in keratinocyte haptotactic migration dependent on alpha 3 beta 1 integrin. J Cell Biol. 2001;153:465-78 pubmed
Silletti S, Mei F, Sheppard D, Montgomery A. Plasmin-sensitive dibasic sequences in the third fibronectin-like domain of L1-cell adhesion molecule (CAM) facilitate homomultimerization and concomitant integrin recruitment. J Cell Biol. 2000;149:1485-502 pubmed
product information
Catalog Number :
MAB1987Z
Subcategory :
Cell Structure
Product Name :
Anti-Integrin β1 Antibody, clone P4C10, azide free
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P05556
Host Name :
Mouse
Antigen :
Integrin β1
Clone :
P4C10
Conjugate :
Purified
Isotype :
IgG2a
Product Description :
Anti-Integrin β1 Antibody, clone P4C10, azide free
Cross Reactivity :
Human
Background :
Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
ALT Names :
CD29;MAB1987
Specificity :
Reacts with the beta-1 subunit of the integrin protein family. Appears to be human specific.Unlike many beta-1 monoclonals 4C10's epitope is not trypsin sensitive.
Package Size :
100 μg
Uses :
ELISA;Immunocytochemistry;Immunoprecipitation;Affects Function;Western Blotting
Storage :
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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