This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Integrin αVβ3 Antibody, clone LM609
catalog :
MAB1976
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
LM609

The same clone is also sold as:
reactivity :
hamsters, Common quail, human, mouse, rat, chicken, bovine, domestic rabbit
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay, blocking or activating experiments
citations: 221
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; human; loading ...; fig 3b
Koh K, Cao Y, Mangos S, Tardi N, Dande R, Lee H, et al. Nonimmune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist. J Clin Invest. 2019;129:1713-1726 pubmed publisher
  • flow cytometry; human; loading ...; fig 1
  • immunocytochemistry; human; loading ...; fig 17
Nestić D, Uil T, Ma J, Roy S, Vellinga J, Baker A, et al. αvβ3 Integrin Is Required for Efficient Infection of Epithelial Cells with Human Adenovirus Type 26. J Virol. 2019;93: pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 4b
Oyama M, Kariya Y, Kariya Y, Matsumoto K, Kanno M, Yamaguchi Y, et al. Biological role of site-specific O-glycosylation in cell adhesion activity and phosphorylation of osteopontin. Biochem J. 2018;475:1583-1595 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s6a
Owen L, Adhikari A, Patel M, Grimmer P, Leijnse N, Kim M, et al. A cytoskeletal clutch mediates cellular force transmission in a soft, three-dimensional extracellular matrix. Mol Biol Cell. 2017;28:1959-1974 pubmed publisher
  • flow cytometry; human; 1:100; loading ...; fig 1a
  • immunohistochemistry; human
Zhang Y, Yang J, Ding M, Li L, Lu Z, Zhang Q, et al. Tumor-penetration and antitumor efficacy of cetuximab are enhanced by co-administered iRGD in a murine model of human NSCLC. Oncol Lett. 2016;12:3241-3249 pubmed
  • western blot; human; fig 6a
Yoo S, Leng L, Kim B, Du X, Tilstam P, Kim K, et al. MIF allele-dependent regulation of the MIF coreceptor CD44 and role in rheumatoid arthritis. Proc Natl Acad Sci U S A. 2016;113:E7917-E7926 pubmed
  • flow cytometry; bovine
Guzman E, Taylor G, Hope J, Herbert R, Cubillos Zapata C, Charleston B. Transduction of skin-migrating dendritic cells by human adenovirus 5 occurs via an actin-dependent phagocytic pathway. J Gen Virol. 2016;97:2703-2718 pubmed publisher
  • flow cytometry; human; tbl 3
Kaliberov S, Kaliberova L, Yan H, Kapoor V, Hallahan D. Retargeted adenoviruses for radiation-guided gene delivery. Cancer Gene Ther. 2016;23:303-14 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 5a
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 7c
Singh A, Fedele C, Lu H, Nevalainen M, Keen J, Languino L. Exosome-mediated Transfer of αvβ3 Integrin from Tumorigenic to Nontumorigenic Cells Promotes a Migratory Phenotype. Mol Cancer Res. 2016;14:1136-1146 pubmed
  • flow cytometry; human; 1:200; loading ...; fig 1d
Gvozdenović A, Boro A, Meier D, Bode Lesniewska B, Born W, Muff R, et al. Targeting αvβ3 and αvβ5 integrins inhibits pulmonary metastasis in an intratibial xenograft osteosarcoma mouse model. Oncotarget. 2016;7:55141-55154 pubmed publisher
  • blocking or activating experiments; human; fig 1
Pijuan Galitó S, Tamm C, Schuster J, Sobol M, Forsberg L, Merry C, et al. Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture. Nat Commun. 2016;7:12170 pubmed publisher
  • immunocytochemistry; human; fig s1
Daubon T, Spuul P, Alonso F, Fremaux I, Genot E. VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells. J Cell Sci. 2016;129:2586-98 pubmed publisher
  • blocking or activating experiments; human; fig 2
Cornick S, Moreau F, Chadee K. Entamoeba histolytica Cysteine Proteinase 5 Evokes Mucin Exocytosis from Colonic Goblet Cells via αvβ3 Integrin. PLoS Pathog. 2016;12:e1005579 pubmed publisher
  • flow cytometry; human; fig 7
  • flow cytometry; hamsters; fig 7
Khan T, Wang X, Maynard J. Inclusion of an RGD Motif Alters Invasin Integrin-Binding Affinity and Specificity. Biochemistry. 2016;55:2078-90 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 4b
Schaufler V, Czichos Medda H, Hirschfeld Warnecken V, Neubauer S, Rechenmacher F, Medda R, et al. Selective binding and lateral clustering of ?5?1 and ?v?3 integrins: Unraveling the spatial requirements for cell spreading and focal adhesion assembly. Cell Adh Migr. 2016;10:505-515 pubmed
  • blocking or activating experiments; human; fig 5
Eriksson J, Le Joncour V, Nummela P, Jahkola T, Virolainen S, Laakkonen P, et al. Gene expression analyses of primary melanomas reveal CTHRC1 as an important player in melanoma progression. Oncotarget. 2016;7:15065-92 pubmed publisher
  • flow cytometry; human; fig 4
  • immunocytochemistry; human; fig 3
Oudart J, Doué M, Vautrin A, Brassart B, Sellier C, Dupont Deshorgue A, et al. The anti-tumor NC1 domain of collagen XIX inhibits the FAK/ PI3K/Akt/mTOR signaling pathway through αvβ3 integrin interaction. Oncotarget. 2016;7:1516-28 pubmed publisher
  • proximity ligation assay; human; 1:1000; fig s3
Fiore V, Strane P, Bryksin A, White E, Hagood J, Barker T. Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction. J Cell Biol. 2015;211:173-90 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 6
Zoppi N, Chiarelli N, Cinquina V, Ritelli M, Colombi M. GLUT10 deficiency leads to oxidative stress and non-canonical αvβ3 integrin-mediated TGFβ signalling associated with extracellular matrix disarray in arterial tortuosity syndrome skin fibroblasts. Hum Mol Genet. 2015;24:6769-87 pubmed publisher
  • western blot; human
Mori S, Kodaira M, Ito A, Okazaki M, Kawaguchi N, Hamada Y, et al. Enhanced Expression of Integrin αvβ3 Induced by TGF-β Is Required for the Enhancing Effect of Fibroblast Growth Factor 1 (FGF1) in TGF-β-Induced Epithelial-Mesenchymal Transition (EMT) in Mammary Epithelial Cells. PLoS ONE. 2015;10:e0137486 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 2a
  • flow cytometry; human; loading ...; fig 2b
Martin A, Cardoso A, Selistre de Araujo H, Cominetti M. Recombinant disintegrin domain of human ADAM9 inhibits migration and invasion of DU145 prostate tumor cells. Cell Adh Migr. 2015;9:293-9 pubmed publisher
  • flow cytometry; human
Krishnan S, Szabo E, Burghardt I, Frei K, Tabatabai G, Weller M. Modulation of cerebral endothelial cell function by TGF-β in glioblastoma: VEGF-dependent angiogenesis versus endothelial mesenchymal transition. Oncotarget. 2015;6:22480-95 pubmed
  • blocking or activating experiments; human; fig 5
Ross J, Huh D, Noble L, Tavazoie S. Identification of molecular determinants of primary and metastatic tumour re-initiation in breast cancer. Nat Cell Biol. 2015;17:651-64 pubmed publisher
  • blocking or activating experiments; rat
Oliveira C, Marqueti R, Cominetti M, Douat E, Ribeiro J, Pontes C, et al. Effects of blocking αvβ3 integrin by a recombinant RGD disintegrin on remodeling of wound healing after induction of incisional hernia in rats. Acta Cir Bras. 2015;30:134-42 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:800
Withofs N, Signolle N, Somja J, Lovinfosse P, Nzaramba E, Mievis F, et al. 18F-FPRGD2 PET/CT imaging of integrin αvβ3 in renal carcinomas: correlation with histopathology. J Nucl Med. 2015;56:361-4 pubmed publisher
  • immunocytochemistry; human; 1:40; fig 3
Hakanpaa L, Sipilä T, Leppänen V, Gautam P, Nurmi H, Jacquemet G, et al. Endothelial destabilization by angiopoietin-2 via integrin β1 activation. Nat Commun. 2015;6:5962 pubmed publisher
  • ELISA; Common quail; 1:1000; loading ...; fig 2
Scotti L, Abramovich D, Pascuali N, Durand L, Irusta G, de Zúñiga I, et al. Inhibition of angiopoietin-1 (ANGPT1) affects vascular integrity in ovarian hyperstimulation syndrome (OHSS). Reprod Fertil Dev. 2016;28:690-9 pubmed publisher
  • flow cytometry; human; fig 1
Sugahara K, Braun G, de Mendoza T, Kotamraju V, French R, Lowy A, et al. Tumor-penetrating iRGD peptide inhibits metastasis. Mol Cancer Ther. 2015;14:120-8 pubmed publisher
  • flow cytometry; human
Horiguchi H, Endo M, Miyamoto Y, Sakamoto Y, Odagiri H, Masuda T, et al. Angiopoietin-like protein 2 renders colorectal cancer cells resistant to chemotherapy by activating spleen tyrosine kinase-phosphoinositide 3-kinase-dependent anti-apoptotic signaling. Cancer Sci. 2014;105:1550-9 pubmed publisher
  • immunohistochemistry - frozen section; human
Contois L, Akalu A, Caron J, Tweedie E, Cretu A, Henderson T, et al. Inhibition of tumor-associated αvβ3 integrin regulates the angiogenic switch by enhancing expression of IGFBP-4 leading to reduced melanoma growth and angiogenesis in vivo. Angiogenesis. 2015;18:31-46 pubmed publisher
  • immunocytochemistry; human
Cohen K, Flint N, Shalev S, Erez D, Baharal T, Davis P, et al. Thyroid hormone regulates adhesion, migration and matrix metalloproteinase 9 activity via ?v?3 integrin in myeloma cells. Oncotarget. 2014;5:6312-22 pubmed
  • immunohistochemistry; human
Boehlke C, Hartleben B, Huber T, Hopfer H, Walz G, Neumann Haefelin E. Hantavirus infection with severe proteinuria and podocyte foot-process effacement. Am J Kidney Dis. 2014;64:452-6 pubmed publisher
  • flow cytometry; human; 1:100
Faralli J, Gagen D, Filla M, Crotti T, Peters D. Dexamethasone increases ?v?3 integrin expression and affinity through a calcineurin/NFAT pathway. Biochim Biophys Acta. 2013;1833:3306-3313 pubmed publisher
  • flow cytometry; mouse; 1:200
Zhao Y, Wang S, Sorenson C, Teixeira L, Dubielzig R, Peters D, et al. Cyp1b1 mediates periostin regulation of trabecular meshwork development by suppression of oxidative stress. Mol Cell Biol. 2013;33:4225-40 pubmed publisher
  • immunocytochemistry; human; 1:100
Chen C, Yeh M, Shiau C, Chiang C, Lu D. Efficient downregulation of VEGF in retinal pigment epithelial cells by integrin ligand-labeled liposome-mediated siRNA delivery. Int J Nanomedicine. 2013;8:2613-27 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
Zhang C, Yang C, Wang R, Jiao Y, Ampah K, Wang X, et al. c-Abl Kinase Is a Regulator of ?v?3 Integrin Mediated Melanoma A375 Cell Migration. PLoS ONE. 2013;8:e66108 pubmed publisher
  • blocking or activating experiments; human
Riches K, Franklin L, Maqbool A, Peckham M, Adams M, Bond J, et al. Apolipoprotein(a) acts as a chemorepellent to human vascular smooth muscle cells via integrin ?V?3 and RhoA/ROCK-mediated mechanisms. Int J Biochem Cell Biol. 2013;45:1776-83 pubmed publisher
  • flow cytometry; human
Wang J, Wang L, Xia B, Yang C, Lai H, Chen X. BSP gene silencing inhibits migration, invasion, and bone metastasis of MDA-MB-231BO human breast cancer cells. PLoS ONE. 2013;8:e62936 pubmed publisher
  • immunocytochemistry; domestic rabbit
Seo Y, Park J, Song C, Kwon S. Comparison of light-emitting diode wavelength on activity and migration of rabbit ACL cells. Lasers Med Sci. 2014;29:245-55 pubmed publisher
  • blocking or activating experiments; mouse
Lin L, Grimme J, Sun J, Lu S, Gai L, Cropek D, et al. The antagonistic roles of PDGF and integrin ?v?3 in regulating ROS production at focal adhesions. Biomaterials. 2013;34:3807-15 pubmed publisher
  • blocking or activating experiments; human
Cabarcas S, Sun L, Mathews L, Thomas S, Zhang X, Farrar W. The differentiation of pancreatic tumor-initiating cells by vitronectin can be blocked by cilengitide. Pancreas. 2013;42:861-70 pubmed publisher
Soriano A, De Cristofaro T, Di Palma T, Dotolo S, Gokulnath P, Izzo A, et al. PAX8 expression in high-grade serous ovarian cancer positively regulates attachment to ECM via Integrin β3. Cancer Cell Int. 2019;19:303 pubmed publisher
Thinn A, Wang Z, Zhou D, Zhao Y, Curtis B, Zhu J. Autonomous conformational regulation of β3 integrin and the conformation-dependent property of HPA-1a alloantibodies. Proc Natl Acad Sci U S A. 2018;115:E9105-E9114 pubmed publisher
Galbraith C, Davidson M, Galbraith J. Coupling integrin dynamics to cellular adhesion behaviors. Biol Open. 2018;7: pubmed publisher
Dudvarski Stankovic N, Bicker F, Keller S, Jones D, Harter P, Kienzle A, et al. EGFL7 enhances surface expression of integrin α5β1 to promote angiogenesis in malignant brain tumors. EMBO Mol Med. 2018;10: pubmed publisher
Yosef G, Arkadash V, Papo N. Targeting the MMP-14/MMP-2/integrin αvβ3 axis with multispecific N-TIMP2-based antagonists for cancer therapy. J Biol Chem. 2018;293:13310-13326 pubmed publisher
Lugano R, Vemuri K, Yu D, Bergqvist M, Smits A, Essand M, et al. CD93 promotes β1 integrin activation and fibronectin fibrillogenesis during tumor angiogenesis. J Clin Invest. 2018;128:3280-3297 pubmed publisher
Sun Q, Lesperance J, Wettersten H, Luterstein E, Derose Y, Welm A, et al. Proapoptotic PUMA targets stem-like breast cancer cells to suppress metastasis. J Clin Invest. 2018;128:531-544 pubmed publisher
Stockis J, Li nart S, Colau D, Collignon A, Nishimura S, Sheppard D, et al. Blocking immunosuppression by human Tregs in vivo with antibodies targeting integrin αVβ8. Proc Natl Acad Sci U S A. 2017;114:E10161-E10168 pubmed publisher
Jessen T, Jessen J. VANGL2 interacts with integrin αv to regulate matrix metalloproteinase activity and cell adhesion to the extracellular matrix. Exp Cell Res. 2017;361:265-276 pubmed publisher
Portillo J, Muniz Feliciano L, Lopez Corcino Y, Lee S, Van Grol J, Parsons S, et al. Toxoplasma gondii induces FAK-Src-STAT3 signaling during infection of host cells that prevents parasite targeting by autophagy. PLoS Pathog. 2017;13:e1006671 pubmed publisher
Bondu V, Wu C, Cao W, Simons P, Gillette J, Zhu J, et al. Low-affinity binding in cis to P2Y2R mediates force-dependent integrin activation during hantavirus infection. Mol Biol Cell. 2017;28:2887-2903 pubmed publisher
Saitoh S, Maruyama T, Yako Y, Kajita M, Fujioka Y, Ohba Y, et al. Rab5-regulated endocytosis plays a crucial role in apical extrusion of transformed cells. Proc Natl Acad Sci U S A. 2017;114:E2327-E2336 pubmed publisher
Horiguchi H, Endo M, Kawane K, Kadomatsu T, Terada K, Morinaga J, et al. ANGPTL2 expression in the intestinal stem cell niche controls epithelial regeneration and homeostasis. EMBO J. 2017;36:409-424 pubmed publisher
Lamason R, Bastounis E, Kafai N, Serrano R, Del Alamo J, Theriot J, et al. Rickettsia Sca4 Reduces Vinculin-Mediated Intercellular Tension to Promote Spread. Cell. 2016;167:670-683.e10 pubmed publisher
Merilahti P, Tauriainen S, Susi P. Human Parechovirus 1 Infection Occurs via αVβ1 Integrin. PLoS ONE. 2016;11:e0154769 pubmed publisher
Symonds J, Ohm A, Tan A, Reyland M. PKCδ regulates integrin αVβ3 expression and transformed growth of K-ras dependent lung cancer cells. Oncotarget. 2016;7:17905-19 pubmed publisher
Ng D, Humphries J, Byron A, Millon Frémillon A, Humphries M. Microtubule-dependent modulation of adhesion complex composition. PLoS ONE. 2014;9:e115213 pubmed publisher
Nakamura T, Shinriki S, Jono H, Ueda M, Nagata M, Guo J, et al. Osteopontin-integrin α(v)β(3) axis is crucial for 5-fluorouracil resistance in oral squamous cell carcinoma. FEBS Lett. 2015;589:231-9 pubmed publisher
Haubner R, Maschauer S, Einsiedel J, Eder I, Rangger C, Gmeiner P, et al. H-CRRETAWAC-OH, a lead structure for the development of radiotracer targeting integrin α5β1?. Biomed Res Int. 2014;2014:243185 pubmed publisher
Klingberg F, Chow M, Koehler A, Boo S, Buscemi L, Quinn T, et al. Prestress in the extracellular matrix sensitizes latent TGF-β1 for activation. J Cell Biol. 2014;207:283-97 pubmed publisher
Li X, Liu Y, Haas T. Peptides derived from central turn motifs within integrin αIIb and αV cytoplasmic tails inhibit integrin activation. Peptides. 2014;62:38-48 pubmed publisher
Rylova S, Barnucz E, Fani M, Braun F, Werner M, Lassmann S, et al. Does imaging αvβ3 integrin expression with PET detect changes in angiogenesis during bevacizumab therapy?. J Nucl Med. 2014;55:1878-84 pubmed publisher
Hsiao C, Wang W, Kuo W, Chen H, Chen T, Hamann J, et al. CD97 inhibits cell migration in human fibrosarcoma cells by modulating TIMP-2/MT1- MMP/MMP-2 activity--role of GPS autoproteolysis and functional cooperation between the N- and C-terminal fragments. FEBS J. 2014;281:4878-91 pubmed publisher
Morjen M, Honoré S, Bazaa A, Abdelkafi Koubaa Z, Ellafi A, Mabrouk K, et al. PIVL, a snake venom Kunitz-type serine protease inhibitor, inhibits in vitro and in vivo angiogenesis. Microvasc Res. 2014;95:149-56 pubmed publisher
Zhan Y, Yu B, Wang Z, Zhang Y, Zhang H, Wu H, et al. A fiber-modified adenovirus co-expressing HSV-TK and Coli.NTR enhances antitumor activities in breast cancer cells. Int J Clin Exp Pathol. 2014;7:2850-60 pubmed
Fuhrmann A, Li J, Chien S, Engler A. Cation type specific cell remodeling regulates attachment strength. PLoS ONE. 2014;9:e102424 pubmed publisher
Kariya Y, Kanno M, Matsumoto Morita K, Konno M, Yamaguchi Y, Hashimoto Y. Osteopontin O-glycosylation contributes to its phosphorylation and cell-adhesion properties. Biochem J. 2014;463:93-102 pubmed publisher
Rui X, Mehrbod M, Van Agthoven J, Anand S, Xiong J, Mofrad M, et al. The ?-subunit regulates stability of the metal ion at the ligand-associated metal ion-binding site in ?3 integrins. J Biol Chem. 2014;289:23256-63 pubmed publisher
Nakajima T, Hirata M, Shearer T, Azuma M. Mechanism for laser-induced neovascularization in rat choroid: accumulation of integrin ? chain-positive cells and their ligands. Mol Vis. 2014;20:864-71 pubmed
Janib S, Gustafson J, Minea R, Swenson S, Liu S, Pastuszka M, et al. Multimeric disintegrin protein polymer fusions that target tumor vasculature. Biomacromolecules. 2014;15:2347-58 pubmed publisher
Ray A, Schaffner F, Janouskova H, Noulet F, Rognan D, Lelong Rebel I, et al. Single cell tracking assay reveals an opposite effect of selective small non-peptidic ?5?1 or ?v?3/?5 integrin antagonists in U87MG glioma cells. Biochim Biophys Acta. 2014;1840:2978-87 pubmed publisher
Klegerman M, Zou Y, Golunski E, Peng T, Huang S, McPherson D. Use of thermodynamic coupling between antibody-antigen binding and phospholipid acyl chain phase transition energetics to predict immunoliposome targeting affinity. J Liposome Res. 2014;24:216-22 pubmed publisher
Mor Cohen R, Rosenberg N, Averbukh Y, Seligsohn U, Lahav J. Disulfide bond exchanges in integrins ?IIb?3 and ?v?3 are required for activation and post-ligation signaling during clot retraction. Thromb Res. 2014;133:826-36 pubmed publisher
Podolnikova N, Yakovlev S, Yakubenko V, Wang X, Gorkun O, Ugarova T. The interaction of integrin ?IIb?3 with fibrin occurs through multiple binding sites in the ?IIb ?-propeller domain. J Biol Chem. 2014;289:2371-83 pubmed publisher
Aulakh G, Balachandran Y, Liu L, Singh B. Angiostatin inhibits activation and migration of neutrophils. Cell Tissue Res. 2014;355:375-96 pubmed publisher
Belcher J, Chen C, Nguyen J, Milbauer L, Abdulla F, Alayash A, et al. Heme triggers TLR4 signaling leading to endothelial cell activation and vaso-occlusion in murine sickle cell disease. Blood. 2014;123:377-90 pubmed publisher
Wang R, Bi J, Ampah K, Ba X, Liu W, Zeng X. Lipid rafts control human melanoma cell migration by regulating focal adhesion disassembly. Biochim Biophys Acta. 2013;1833:3195-3205 pubmed publisher
Tome Y, Kimura H, Maehara H, Sugimoto N, Bouvet M, Tsuchiya H, et al. High lung-metastatic variant of human osteosarcoma cells, selected by passage of lung metastasis in nude mice, is associated with increased expression of ?(v)?(3) integrin. Anticancer Res. 2013;33:3623-7 pubmed
Bifulco K, Longanesi Cattani I, Liguori E, Arra C, Rea D, Masucci M, et al. A urokinase receptor-derived peptide inhibiting VEGF-dependent directional migration and vascular sprouting. Mol Cancer Ther. 2013;12:1981-93 pubmed publisher
Serres E, Debarbieux F, Stanchi F, Maggiorella L, Grall D, Turchi L, et al. Fibronectin expression in glioblastomas promotes cell cohesion, collective invasion of basement membrane in vitro and orthotopic tumor growth in mice. Oncogene. 2014;33:3451-62 pubmed publisher
Otagiri D, Yamada Y, Hozumi K, Katagiri F, Kikkawa Y, Nomizu M. Cell attachment and spreading activity of mixed laminin peptide-chitosan membranes. Biopolymers. 2013;100:751-9 pubmed publisher
Gagen D, Filla M, Clark R, LITON P, Peters D. Activated ?v?3 integrin regulates ?v?5 integrin-mediated phagocytosis in trabecular meshwork cells. Invest Ophthalmol Vis Sci. 2013;54:5000-11 pubmed publisher
Schmieder A, Winter P, Williams T, Allen J, Hu G, Zhang H, et al. Molecular MR imaging of neovascular progression in the Vx2 tumor with ?v?3-targeted paramagnetic nanoparticles. Radiology. 2013;268:470-80 pubmed publisher
Long Q, Zhou M, Liu X, Du Y, Fan J, Li X, et al. Interaction of CCN1 with ?v?3 integrin induces P-glycoprotein and confers vinblastine resistance in renal cell carcinoma cells. Anticancer Drugs. 2013;24:810-7 pubmed publisher
Dall Angelo S, Zhang Q, Fleming I, Piras M, Schweiger L, O Hagan D, et al. Efficient bioconjugation of 5-fluoro-5-deoxy-ribose (FDR) to RGD peptides for positron emission tomography (PET) imaging of ?(v)?(3) integrin receptor. Org Biomol Chem. 2013;11:4551-8 pubmed publisher
Traub S, Morgner J, Martino M, Honing S, Swartz M, Wickström S, et al. The promotion of endothelial cell attachment and spreading using FNIII10 fused to VEGF-A165. Biomaterials. 2013;34:5958-68 pubmed publisher
Wang R, Bi J, Ampah K, Zhang C, Li Z, Jiao Y, et al. Lipid raft regulates the initial spreading of melanoma A375 cells by modulating ?1 integrin clustering. Int J Biochem Cell Biol. 2013;45:1679-89 pubmed publisher
Jim Leu S, Sung J, Huang M, Chen M, Tsai T. A novel anti-CCN1 monoclonal antibody suppresses Rac-dependent cytoskeletal reorganization and migratory activities in breast cancer cells. Biochem Biophys Res Commun. 2013;434:885-91 pubmed publisher
Conti L, Lanzardo S, Iezzi M, Montone M, Bolli E, Brioschi C, et al. Optical imaging detection of microscopic mammary cancer in ErbB-2 transgenic mice through the DA364 probe binding ?v ?3 integrins. Contrast Media Mol Imaging. 2013;8:350-60 pubmed publisher
Ruiz Ontañon P, Orgaz J, Aldaz B, Elosegui Artola A, Martino J, Berciano M, et al. Cellular plasticity confers migratory and invasive advantages to a population of glioblastoma-initiating cells that infiltrate peritumoral tissue. Stem Cells. 2013;31:1075-85 pubmed publisher
Rapraeger A, Ell B, Roy M, Li X, Morrison O, Thomas G, et al. Vascular endothelial-cadherin stimulates syndecan-1-coupled insulin-like growth factor-1 receptor and cross-talk between ?V?3 integrin and vascular endothelial growth factor receptor 2 at the onset of endothelial cell dissemination during angiogenesi. FEBS J. 2013;280:2194-206 pubmed publisher
Whitson R, Lucia M, Lambert J. Growth differentiation factor-15 (GDF-15) suppresses in vitro angiogenesis through a novel interaction with connective tissue growth factor (CCN2). J Cell Biochem. 2013;114:1424-33 pubmed publisher
Kemeny S, Cicalese S, Figueroa D, Clyne A. Glycated collagen and altered glucose increase endothelial cell adhesion strength. J Cell Physiol. 2013;228:1727-36 pubmed publisher
Krautkrämer E, Lehmann M, Bollinger V, Zeier M. Polar release of pathogenic Old World hantaviruses from renal tubular epithelial cells. Virol J. 2012;9:299 pubmed publisher
Georgoulis A, Havaki S, Drosos Y, Goutas N, Vlachodimitropoulos D, Aleporou Marinou V, et al. RGD binding to integrin alphavbeta3 affects cell motility and adhesion in primary human breast cancer cultures. Ultrastruct Pathol. 2012;36:387-99 pubmed publisher
Majhen D, Richardson J, Vukelić B, Dodig I, Cindric M, Benihoud K, et al. The disulfide bond of an RGD4C motif inserted within the Hi loop of the adenovirus type 5 fiber protein is critical for retargeting to ?v -integrins. J Gene Med. 2012;14:788-97 pubmed publisher
Eberlein C, Kendrew J, McDaid K, Alfred A, Kang J, Jacobs V, et al. A human monoclonal antibody 264RAD targeting ?v?6 integrin reduces tumour growth and metastasis, and modulates key biomarkers in vivo. Oncogene. 2013;32:4406-16 pubmed publisher
Dong X, Mi L, Zhu J, Wang W, Hu P, Luo B, et al. ?(V)?(3) integrin crystal structures and their functional implications. Biochemistry. 2012;51:8814-28 pubmed publisher
Ravelli C, Mitola S, Corsini M, Presta M. Involvement of ?v?3 integrin in gremlin-induced angiogenesis. Angiogenesis. 2013;16:235-43 pubmed publisher
Manetti M, Guiducci S, Romano E, Rosa I, Ceccarelli C, Mello T, et al. Differential expression of junctional adhesion molecules in different stages of systemic sclerosis. Arthritis Rheum. 2013;65:247-57 pubmed publisher
Iida J, Dorchak J, Lehman J, Clancy R, Luo C, Chen Y, et al. FH535 inhibited migration and growth of breast cancer cells. PLoS ONE. 2012;7:e44418 pubmed publisher
Cox R, Livesay S, Johnson M, Ohi M, Williams J. The human metapneumovirus fusion protein mediates entry via an interaction with RGD-binding integrins. J Virol. 2012;86:12148-60 pubmed publisher
Lescarbeau R, Seib F, Prewitz M, Werner C, Kaplan D. In vitro model of metastasis to bone marrow mediates prostate cancer castration resistant growth through paracrine and extracellular matrix factors. PLoS ONE. 2012;7:e40372 pubmed publisher
Veron D, Villegas G, Aggarwal P, Bertuccio C, Jimenez J, Velazquez H, et al. Acute podocyte vascular endothelial growth factor (VEGF-A) knockdown disrupts alphaVbeta3 integrin signaling in the glomerulus. PLoS ONE. 2012;7:e40589 pubmed publisher
Zoppi N, Ritelli M, Colombi M. Type III and V collagens modulate the expression and assembly of EDA(+) fibronectin in the extracellular matrix of defective Ehlers-Danlos syndrome fibroblasts. Biochim Biophys Acta. 2012;1820:1576-87 pubmed publisher
Jin Z, Furukawa T, Claron M, Boturyn D, Coll J, Fukumura T, et al. Positron emission tomography imaging of tumor angiogenesis and monitoring of antiangiogenic efficacy using the novel tetrameric peptide probe 64Cu-cyclam-RAFT-c(-RGDfK-)4. Angiogenesis. 2012;15:569-80 pubmed publisher
Hahn A, Kaufmann J, Wies E, Naschberger E, Panteleev Ivlev J, Schmidt K, et al. The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma–associated herpesvirus. Nat Med. 2012;18:961-6 pubmed publisher
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product information
Catalog Number :
MAB1976
Subcategory :
Cell Structure
Product Name :
Anti-Integrin αVβ3 Antibody, clone LM609
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P06756
Host Name :
Mouse
Antigen :
Integrin αVβ3
Clone :
LM609
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-Integrin αVβ3 Antibody, clone LM609
Cross Reactivity :
Avian;Bovine;Canine;Chicken;Human;Monkey;Pig;Rabbit
Background :
The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue (Brooks, 1994). Due to its involvement in angiogenesis, integrin alphaVbeta3 is one of the most intensely studied of the integrin receptors.
ALT Names :
Vitronectin Receptor; CD51/CD61
Immunogen :
Purified adhesion receptor from the human melanoma cell line M21 (Cheresh, 1987).
Specificity :
Reacts with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, 1987). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, 1994). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits.
Package Size :
100 μg
Uses :
Flow Cytometry;Immunofluorescence;Immunohistochemistry;Immunoprecipitation;Affects Function
Storage :
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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