This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Integrin α1 Antibody, clone FB12, azide free
catalog :
MAB1973Z
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
FB12
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
citations: 39
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; fig s6
Gerber T, Willscher E, Loeffler Wirth H, Hopp L, Schadendorf D, Schartl M, et al. Mapping heterogeneity in patient-derived melanoma cultures by single-cell RNA-seq. Oncotarget. 2017;8:846-862 pubmed publisher
  • blocking or activating experiments; human; fig 3
Artym V, Swatkoski S, Matsumoto K, Campbell C, Petrie R, Dimitriadis E, et al. Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network. J Cell Biol. 2015;208:331-50 pubmed publisher
  • blocking or activating experiments; human; 10 ug/ml
  • flow cytometry; human; 2 ug/25 x104cells
Stampolidis P, Ullrich A, Iacobelli S. LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention. Oncogene. 2015;34:39-52 pubmed publisher
  • flow cytometry; human
Iwai L, Payne L, Luczynski M, Chang F, Xu H, Clinton R, et al. Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants. Biochem J. 2013;454:501-13 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:100
  • flow cytometry; human
Bridgen D, Gilchrist C, Richardson W, Isaacs R, Brown C, Yang K, et al. Integrin-mediated interactions with extracellular matrix proteins for nucleus pulposus cells of the human intervertebral disc. J Orthop Res. 2013;31:1661-7 pubmed publisher
  • immunocytochemistry; human
Ho Y, Kok S, Wang J, Lin L. Translucent titanium coating altered the composition of focal adhesions and promoted migration of osteoblast-like MG-63 cells on glass. J Biomed Mater Res A. 2014;102:1187-201 pubmed publisher
  • western blot; human; 1:500
Burkin H, Rice M, Sarathy A, Thompson S, Singer C, Buxton I. Integrin upregulation and localization to focal adhesion sites in pregnant human myometrium. Reprod Sci. 2013;20:804-12 pubmed publisher
  • blocking or activating experiments; human; 10 ug/ml
  • immunocytochemistry; human; 10 ug/ml
Xu H, Bihan D, Chang F, Huang P, Farndale R, Leitinger B. Discoidin domain receptors promote α1β1- and α2β1-integrin mediated cell adhesion to collagen by enhancing integrin activation. PLoS ONE. 2012;7:e52209 pubmed publisher
Katagiri F, Hara T, Yamada Y, Urushibata S, Hozumi K, Kikkawa Y, et al. Biological activities of the homologous loop regions in the laminin ? chain LG modules. Biochemistry. 2014;53:3699-708 pubmed publisher
Katagiri F, Takagi M, Nakamura M, Tanaka Y, Hozumi K, Kikkawa Y, et al. Screening of integrin-binding peptides in a laminin peptide library derived from the mouse laminin ? chain short arm regions. Arch Biochem Biophys. 2014;550-551:33-41 pubmed publisher
Meinhardt G, Haider S, Haslinger P, Proestling K, Fiala C, Pollheimer J, et al. Wnt-dependent T-cell factor-4 controls human etravillous trophoblast motility. Endocrinology. 2014;155:1908-20 pubmed publisher
Otagiri D, Yamada Y, Hozumi K, Katagiri F, Kikkawa Y, Nomizu M. Cell attachment and spreading activity of mixed laminin peptide-chitosan membranes. Biopolymers. 2013;100:751-9 pubmed publisher
Bazan Socha S, Zuk J, Plutecka H, Marcinkiewicz C, Zareba L, Musial J. Collagen receptors ?(1)?(1) and ?(2)?(1) integrins are involved in transmigration of peripheral blood eosinophils, but not mononuclear cells through human microvascular endothelial cells monolayer. J Physiol Pharmacol. 2012;63:373-9 pubmed
Prutsch N, Fock V, Haslinger P, Haider S, Fiala C, Pollheimer J, et al. The role of interleukin-1? in human trophoblast motility. Placenta. 2012;33:696-703 pubmed publisher
Hozumi K, Ishikawa M, Hayashi T, Yamada Y, Katagiri F, Kikkawa Y, et al. Identification of cell adhesive sequences in the N-terminal region of the laminin ?2 chain. J Biol Chem. 2012;287:25111-22 pubmed publisher
Katagiri F, Ishikawa M, Yamada Y, Hozumi K, Kikkawa Y, Nomizu M. Screening of integrin-binding peptides from the laminin ?4 and ?5 chain G domain peptide library. Arch Biochem Biophys. 2012;521:32-42 pubmed publisher
Liu G, Oudit G, Fang F, Zhou J, Scholey J. Angiotensin-(1-7)-induced activation of ERK1/2 is cAMP/protein kinase A-dependent in glomerular mesangial cells. Am J Physiol Renal Physiol. 2012;302:F784-90 pubmed publisher
Anton L, Brown A, Parry S, Elovitz M. Lipopolysaccharide induces cytokine production and decreases extravillous trophoblast invasion through a mitogen-activated protein kinase-mediated pathway: possible mechanisms of first trimester placental dysfunction. Hum Reprod. 2012;27:61-72 pubmed publisher
Kikkawa Y, Miwa T, Tohara Y, Hamakubo T, Nomizu M. An antibody to the lutheran glycoprotein (Lu) recognizing the LU4 blood type variant inhibits cell adhesion to laminin ?5. PLoS ONE. 2011;6:e23329 pubmed publisher
Yamada Y, Katagiri F, Hozumi K, Kikkawa Y, Nomizu M. Cell behavior on protein matrices containing laminin ?1 peptide AG73. Biomaterials. 2011;32:4327-35 pubmed publisher
Steele M, Schieler A, Kelley P, Tempero R. ?1 integrin regulates MMP-10 dependant tubulogenesis in human lymphatic endothelial cells. Matrix Biol. 2011;30:218-24 pubmed publisher
Biadasiewicz K, Sonderegger S, Haslinger P, Haider S, Saleh L, Fiala C, et al. Transcription factor AP-2? promotes EGF-dependent invasion of human trophoblast. Endocrinology. 2011;152:1458-69 pubmed publisher
Ratzinger S, Grassel S, Dowejko A, Reichert T, Bauer R. Induction of type XVI collagen expression facilitates proliferation of oral cancer cells. Matrix Biol. 2011;30:118-25 pubmed publisher
Teo J, Ng R, Ng C, Lin A. Surface characteristics of acrylic modified polysulfone membranes improves renal proximal tubule cell adhesion and spreading. Acta Biomater. 2011;7:2060-9 pubmed publisher
Adachi M, Nasu K, Tsuno A, Yuge A, Kawano Y, Narahara H. Attachment to extracellular matrices is enhanced in human endometriotic stromal cells: a possible mechanism underlying the pathogenesis of endometriosis. Eur J Obstet Gynecol Reprod Biol. 2011;155:85-8 pubmed publisher
Hozumi K, Akizuki T, Yamada Y, Hara T, Urushibata S, Katagiri F, et al. Cell adhesive peptide screening of the mouse laminin ?1 chain G domain. Arch Biochem Biophys. 2010;503:213-22 pubmed publisher
Chambrion C, Le Naour F. The tetraspanins CD9 and CD81 regulate CD9P1-induced effects on cell migration. PLoS ONE. 2010;5:e11219 pubmed publisher
Irigoyen M, Pajares M, Agorreta J, Ponz Sarvisé M, Salvo E, Lozano M, et al. TGFBI expression is associated with a better response to chemotherapy in NSCLC. Mol Cancer. 2010;9:130 pubmed publisher
Sonderegger S, Haslinger P, Sabri A, Leisser C, Otten J, Fiala C, et al. Wingless (Wnt)-3A induces trophoblast migration and matrix metalloproteinase-2 secretion through canonical Wnt signaling and protein kinase B/AKT activation. Endocrinology. 2010;151:211-20 pubmed publisher
Stratman A, Malotte K, Mahan R, Davis M, Davis G. Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation. Blood. 2009;114:5091-101 pubmed publisher
Tsuno A, Nasu K, Yuge A, Matsumoto H, Nishida M, Narahara H. Decidualization attenuates the contractility of eutopic and ectopic endometrial stromal cells: implications for hormone therapy of endometriosis. J Clin Endocrinol Metab. 2009;94:2516-23 pubmed publisher
Stratman A, Saunders W, Sacharidou A, Koh W, Fisher K, Zawieja D, et al. Endothelial cell lumen and vascular guidance tunnel formation requires MT1-MMP-dependent proteolysis in 3-dimensional collagen matrices. Blood. 2009;114:237-47 pubmed publisher
Irigoyen M, Anso E, Salvo E, Dotor de las Herrerías J, Martinez Irujo J, Rouzaut A. TGFbeta-induced protein mediates lymphatic endothelial cell adhesion to the extracellular matrix under low oxygen conditions. Cell Mol Life Sci. 2008;65:2244-55 pubmed publisher
Johnson K, Rose D, Terkeltaub R. Factor XIIIA mobilizes transglutaminase 2 to induce chondrocyte hypertrophic differentiation. J Cell Sci. 2008;121:2256-64 pubmed publisher
Bolduc G, Madoff L. The group B streptococcal alpha C protein binds alpha1beta1-integrin through a novel KTD motif that promotes internalization of GBS within human epithelial cells. Microbiology. 2007;153:4039-49 pubmed
Gilchrist C, Chen J, Richardson W, Loeser R, Setton L. Functional integrin subunits regulating cell-matrix interactions in the intervertebral disc. J Orthop Res. 2007;25:829-40 pubmed
Wright S, Malinin N, Powell K, Yednock T, Rydel R, Griswold Prenner I. Alpha2beta1 and alphaVbeta1 integrin signaling pathways mediate amyloid-beta-induced neurotoxicity. Neurobiol Aging. 2007;28:226-37 pubmed
Znoyko I, Trojanowska M, Reuben A. Collagen binding alpha2beta1 and alpha1beta1 integrins play contrasting roles in regulation of Ets-1 expression in human liver myofibroblasts. Mol Cell Biochem. 2006;282:89-99 pubmed
Bix G, Fu J, Gonzalez E, Macro L, Barker A, Campbell S, et al. Endorepellin causes endothelial cell disassembly of actin cytoskeleton and focal adhesions through alpha2beta1 integrin. J Cell Biol. 2004;166:97-109 pubmed
product information
Catalog Number :
MAB1973Z
Subcategory :
Cell Structure
Product Name :
Anti-Integrin α1 Antibody, clone FB12, azide free
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P56199
Host Name :
Mouse
Antigen :
Integrin α1
Clone :
FB12
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-Integrin α1 Antibody, clone FB12, azide free
Cross Reactivity :
Human
ALT Names :
CD49a;MAB1973
Specificity :
Reacts with the I domain (Val151 - Ala364) of human alpha1 integrin (Fabbri, 1996). Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi, 1991). Does not recognize recombinant I domain of rat integrin alpha1.
Package Size :
100 μg
Uses :
ELISA;Flow Cytometry;Immunohistochemistry;Immunoprecipitation;Affects Function;Western Blotting
Storage :
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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