This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Integrin α1 Antibody, clone FB12
catalog :
MAB1973
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
FB12
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments
citations: 40
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000
Velicky P, Haider S, Otti G, Fiala C, Pollheimer J, Knöfler M. Notch-dependent RBPJ? inhibits proliferation of human cytotrophoblasts and their differentiation into extravillous trophoblasts. Mol Hum Reprod. 2014;20:756-66 pubmed publisher
  • blocking or activating experiments; human; 10 ug/ml
  • flow cytometry; human; 2 ug/25 x104cells
Stampolidis P, Ullrich A, Iacobelli S. LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention. Oncogene. 2015;34:39-52 pubmed publisher
  • flow cytometry; human
Iwai L, Payne L, Luczynski M, Chang F, Xu H, Clinton R, et al. Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants. Biochem J. 2013;454:501-13 pubmed publisher
  • immunocytochemistry; human; 1:40
Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M. Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop. BMC Cancer. 2013;13:154 pubmed publisher
  • blocking or activating experiments; human; 10 ug/ml
  • immunocytochemistry; human; 10 ug/ml
Xu H, Bihan D, Chang F, Huang P, Farndale R, Leitinger B. Discoidin domain receptors promote α1β1- and α2β1-integrin mediated cell adhesion to collagen by enhancing integrin activation. PLoS ONE. 2012;7:e52209 pubmed publisher
  • western blot; human; fig 1
Tian W, Qu L, Meng L, Liu C, Wu J, Shou C. Phosphatase of regenerating liver-3 directly interacts with integrin ?1 and regulates its phosphorylation at tyrosine 783. BMC Biochem. 2012;13:22 pubmed publisher
  • flow cytometry; human
Steinberg F, Heesom K, Bass M, Cullen P. SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways. J Cell Biol. 2012;197:219-30 pubmed publisher
Katagiri F, Hara T, Yamada Y, Urushibata S, Hozumi K, Kikkawa Y, et al. Biological activities of the homologous loop regions in the laminin ? chain LG modules. Biochemistry. 2014;53:3699-708 pubmed publisher
Katagiri F, Takagi M, Nakamura M, Tanaka Y, Hozumi K, Kikkawa Y, et al. Screening of integrin-binding peptides in a laminin peptide library derived from the mouse laminin ? chain short arm regions. Arch Biochem Biophys. 2014;550-551:33-41 pubmed publisher
Meinhardt G, Haider S, Haslinger P, Proestling K, Fiala C, Pollheimer J, et al. Wnt-dependent T-cell factor-4 controls human etravillous trophoblast motility. Endocrinology. 2014;155:1908-20 pubmed publisher
Bolás G, de Rezende F, Lorente C, Sanz L, Eble J, Calvete J. Inhibitory effects of recombinant RTS-jerdostatin on integrin ?1?1 function during adhesion, migration and proliferation of rat aortic smooth muscle cells and angiogenesis. Toxicon. 2014;79:45-54 pubmed publisher
Otagiri D, Yamada Y, Hozumi K, Katagiri F, Kikkawa Y, Nomizu M. Cell attachment and spreading activity of mixed laminin peptide-chitosan membranes. Biopolymers. 2013;100:751-9 pubmed publisher
Bridgen D, Gilchrist C, Richardson W, Isaacs R, Brown C, Yang K, et al. Integrin-mediated interactions with extracellular matrix proteins for nucleus pulposus cells of the human intervertebral disc. J Orthop Res. 2013;31:1661-7 pubmed publisher
Bazan Socha S, Zuk J, Plutecka H, Marcinkiewicz C, Zareba L, Musial J. Collagen receptors ?(1)?(1) and ?(2)?(1) integrins are involved in transmigration of peripheral blood eosinophils, but not mononuclear cells through human microvascular endothelial cells monolayer. J Physiol Pharmacol. 2012;63:373-9 pubmed
Prutsch N, Fock V, Haslinger P, Haider S, Fiala C, Pollheimer J, et al. The role of interleukin-1? in human trophoblast motility. Placenta. 2012;33:696-703 pubmed publisher
Hozumi K, Ishikawa M, Hayashi T, Yamada Y, Katagiri F, Kikkawa Y, et al. Identification of cell adhesive sequences in the N-terminal region of the laminin ?2 chain. J Biol Chem. 2012;287:25111-22 pubmed publisher
Katagiri F, Ishikawa M, Yamada Y, Hozumi K, Kikkawa Y, Nomizu M. Screening of integrin-binding peptides from the laminin ?4 and ?5 chain G domain peptide library. Arch Biochem Biophys. 2012;521:32-42 pubmed publisher
Liu G, Oudit G, Fang F, Zhou J, Scholey J. Angiotensin-(1-7)-induced activation of ERK1/2 is cAMP/protein kinase A-dependent in glomerular mesangial cells. Am J Physiol Renal Physiol. 2012;302:F784-90 pubmed publisher
Anton L, Brown A, Parry S, Elovitz M. Lipopolysaccharide induces cytokine production and decreases extravillous trophoblast invasion through a mitogen-activated protein kinase-mediated pathway: possible mechanisms of first trimester placental dysfunction. Hum Reprod. 2012;27:61-72 pubmed publisher
Kikkawa Y, Miwa T, Tohara Y, Hamakubo T, Nomizu M. An antibody to the lutheran glycoprotein (Lu) recognizing the LU4 blood type variant inhibits cell adhesion to laminin ?5. PLoS ONE. 2011;6:e23329 pubmed publisher
Yamada Y, Katagiri F, Hozumi K, Kikkawa Y, Nomizu M. Cell behavior on protein matrices containing laminin ?1 peptide AG73. Biomaterials. 2011;32:4327-35 pubmed publisher
Steele M, Schieler A, Kelley P, Tempero R. ?1 integrin regulates MMP-10 dependant tubulogenesis in human lymphatic endothelial cells. Matrix Biol. 2011;30:218-24 pubmed publisher
Biadasiewicz K, Sonderegger S, Haslinger P, Haider S, Saleh L, Fiala C, et al. Transcription factor AP-2? promotes EGF-dependent invasion of human trophoblast. Endocrinology. 2011;152:1458-69 pubmed publisher
Ishii K, Shimoda M, Sugiura T, Seki K, Takahashi M, Abe M, et al. Involvement of epithelial-mesenchymal transition in adenoid cystic carcinoma metastasis. Int J Oncol. 2011;38:921-31 pubmed publisher
Teo J, Ng R, Ng C, Lin A. Surface characteristics of acrylic modified polysulfone membranes improves renal proximal tubule cell adhesion and spreading. Acta Biomater. 2011;7:2060-9 pubmed publisher
Adachi M, Nasu K, Tsuno A, Yuge A, Kawano Y, Narahara H. Attachment to extracellular matrices is enhanced in human endometriotic stromal cells: a possible mechanism underlying the pathogenesis of endometriosis. Eur J Obstet Gynecol Reprod Biol. 2011;155:85-8 pubmed publisher
Nevo J, Mai A, Tuomi S, Pellinen T, Pentikäinen O, Heikkila P, et al. Mammary-derived growth inhibitor (MDGI) interacts with integrin ?-subunits and suppresses integrin activity and invasion. Oncogene. 2010;29:6452-63 pubmed publisher
Hozumi K, Akizuki T, Yamada Y, Hara T, Urushibata S, Katagiri F, et al. Cell adhesive peptide screening of the mouse laminin ?1 chain G domain. Arch Biochem Biophys. 2010;503:213-22 pubmed publisher
Chambrion C, Le Naour F. The tetraspanins CD9 and CD81 regulate CD9P1-induced effects on cell migration. PLoS ONE. 2010;5:e11219 pubmed publisher
Coelho N, González García C, Planell J, Salmerón Sánchez M, Altankov G. Different assembly of type IV collagen on hydrophilic and hydrophobic substrata alters endothelial cells interaction. Eur Cell Mater. 2010;19:262-72 pubmed
Chen X, Whiting C, Borza C, Hu W, Mont S, Bulus N, et al. Integrin alpha1beta1 regulates epidermal growth factor receptor activation by controlling peroxisome proliferator-activated receptor gamma-dependent caveolin-1 expression. Mol Cell Biol. 2010;30:3048-58 pubmed publisher
Friedrichs J, Helenius J, Muller D. Stimulated single-cell force spectroscopy to quantify cell adhesion receptor crosstalk. Proteomics. 2010;10:1455-62 pubmed publisher
Sonderegger S, Haslinger P, Sabri A, Leisser C, Otten J, Fiala C, et al. Wingless (Wnt)-3A induces trophoblast migration and matrix metalloproteinase-2 secretion through canonical Wnt signaling and protein kinase B/AKT activation. Endocrinology. 2010;151:211-20 pubmed publisher
Tsuno A, Nasu K, Yuge A, Matsumoto H, Nishida M, Narahara H. Decidualization attenuates the contractility of eutopic and ectopic endometrial stromal cells: implications for hormone therapy of endometriosis. J Clin Endocrinol Metab. 2009;94:2516-23 pubmed publisher
Peng Y, Zallocchi M, Meehan D, Delimont D, Chang B, Hawes N, et al. Progressive morphological and functional defects in retinas from alpha1 integrin-null mice. Invest Ophthalmol Vis Sci. 2008;49:4647-54 pubmed publisher
Johnson K, Rose D, Terkeltaub R. Factor XIIIA mobilizes transglutaminase 2 to induce chondrocyte hypertrophic differentiation. J Cell Sci. 2008;121:2256-64 pubmed publisher
Frasca G, Carbonaro V, Merlo S, Copani A, Sortino M. Integrins mediate beta-amyloid-induced cell-cycle activation and neuronal death. J Neurosci Res. 2008;86:350-5 pubmed
Lallier T, Miner Q, Sonnier J, Spencer A. A simple cell motility assay demonstrates differential motility of human periodontal ligament fibroblasts, gingival fibroblasts, and pre-osteoblasts. Cell Tissue Res. 2007;328:339-54 pubmed
Pellinen T, Arjonen A, Vuoriluoto K, Kallio K, Fransen J, Ivaska J. Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins. J Cell Biol. 2006;173:767-80 pubmed
Maginnis M, Forrest J, Kopecky Bromberg S, Dickeson S, Santoro S, Zutter M, et al. Beta1 integrin mediates internalization of mammalian reovirus. J Virol. 2006;80:2760-70 pubmed
product information
Catalog Number :
MAB1973
Subcategory :
Cell Structure
Product Name :
Anti-Integrin α1 Antibody, clone FB12
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P56199
Host Name :
Mouse
Antigen :
Integrin α1
Clone :
FB12
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-Integrin α1 Antibody, clone FB12
Cross Reactivity :
Human
ALT Names :
CD49a
Specificity :
Reacts with the I domain (Val151 - Ala364) of human alpha-1 integrin (CD49a)1. Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi et al., 1991).
Package Size :
100 μg
Uses :
ELISA;Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Affects Function;Western Blotting
Storage :
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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