Integrin α5β1 Antibody, clone JBS5 | EMD Millipore MAB1969 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Integrin α5β1 Antibody, clone JBS5

catalog :

MAB1969

quantity :

100 μL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

JBS5

The same clone is also sold as:

LifeSpan Biosciences: LS-C43331
OriGene: SM1130
Santa Cruz Biotechnology: sc-59762

reactivity :

hamsters, human

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments

citations: 59

Published Application/Species/Sample/Dilution	Reference
flow cytometry; hamsters; fig 7 flow cytometry; human; fig 7	Khan T, Wang X, Maynard J. Inclusion of an RGD Motif Alters Invasin Integrin-Binding Affinity and Specificity. Biochemistry. 2016;55:2078-90 pubmed publisher
flow cytometry; human immunocytochemistry; human	Horiguchi H, Endo M, Miyamoto Y, Sakamoto Y, Odagiri H, Masuda T, et al. Angiopoietin-like protein 2 renders colorectal cancer cells resistant to chemotherapy by activating spleen tyrosine kinase-phosphoinositide 3-kinase-dependent anti-apoptotic signaling. Cancer Sci. 2014;105:1550-9 pubmed publisher
blocking or activating experiments; human	Wei Y, Zhang Y, Cai H, Mirza A, Iorio R, Peeples M, et al. Roles of the putative integrin-binding motif of the human metapneumovirus fusion (f) protein in cell-cell fusion, viral infectivity, and pathogenesis. J Virol. 2014;88:4338-52 pubmed publisher
immunocytochemistry; human; 1:100	Chen C, Yeh M, Shiau C, Chiang C, Lu D. Efficient downregulation of VEGF in retinal pigment epithelial cells by integrin ligand-labeled liposome-mediated siRNA delivery. Int J Nanomedicine. 2013;8:2613-27 pubmed publisher
	Ikeuchi T, de Vega S, Forcinito P, Doyle A, Amaral J, Rodriguez I, et al. Extracellular Protein Fibulin-7 and Its C-Terminal Fragment Have In Vivo Antiangiogenic Activity. Sci Rep. 2018;8:17654 pubmed publisher
	Dudvarski Stankovic N, Bicker F, Keller S, Jones D, Harter P, Kienzle A, et al. EGFL7 enhances surface expression of integrin α5β1 to promote angiogenesis in malignant brain tumors. EMBO Mol Med. 2018;10: pubmed publisher
	Gong X, Didan Y, Lock J, Stromblad S. KIF13A-regulated RhoB plasma membrane localization governs membrane blebbing and blebby amoeboid cell migration. EMBO J. 2018;37: pubmed publisher
	Zhu W, Kim B, Wang M, Huang J, Isak A, Bexiga N, et al. TGFÎ²1 reinforces arterial aging in the vascular smooth muscle cell through a long-range regulation of the cytoskeletal stiffness. Sci Rep. 2018;8:2668 pubmed publisher
	González Tarragó V, Elosegui Artola A, Bazellieres E, Oria R, Pérez González C, Roca Cusachs P. Binding of ZO-1 to ?5?1 integrins regulates the mechanical properties of ?5?1-fibronectin links. Mol Biol Cell. 2017;28:1847-1852 pubmed publisher
	Merilahti P, Tauriainen S, Susi P. Human Parechovirus 1 Infection Occurs via αVβ1 Integrin. PLoS ONE. 2016;11:e0154769 pubmed publisher
	Moran Jones K, Gloss B, Murali R, Chang D, Colvin E, Jones M, et al. Connective tissue growth factor as a novel therapeutic target in high grade serous ovarian cancer. Oncotarget. 2015;6:44551-62 pubmed publisher
	Sugahara K, Braun G, de Mendoza T, Kotamraju V, French R, Lowy A, et al. Tumor-penetrating iRGD peptide inhibits metastasis. Mol Cancer Ther. 2015;14:120-8 pubmed publisher
	Behzad H, Tsai S, Nassab P, Mousavizadeh R, McCormack R, Scott A. Mechanism of mast cell adhesion to human tenocytes in vitro. J Orthop Res. 2015;33:9-16 pubmed publisher
	de Witt S, Swieringa F, Cavill R, Lamers M, van Kruchten R, Mastenbroek T, et al. Identification of platelet function defects by multi-parameter assessment of thrombus formation. Nat Commun. 2014;5:4257 pubmed publisher
	Thinwa J, Segovia J, Bose S, Dube P. Integrin-mediated first signal for inflammasome activation in intestinal epithelial cells. J Immunol. 2014;193:1373-82 pubmed publisher
	Nakajima T, Hirata M, Shearer T, Azuma M. Mechanism for laser-induced neovascularization in rat choroid: accumulation of integrin ? chain-positive cells and their ligands. Mol Vis. 2014;20:864-71 pubmed
	Lee S, Cheng H, Yuan Y, Wu S. Regulation of ionizing radiation-induced adhesion of breast cancer cells to fibronectin by alpha5beta1 integrin. Radiat Res. 2014;181:650-8 pubmed publisher
	Moiseeva E, Straatman K, Leyland M, Bradding P. CADM1 controls actin cytoskeleton assembly and regulates extracellular matrix adhesion in human mast cells. PLoS ONE. 2014;9:e85980 pubmed publisher
	Caffarel M, Chattopadhyay A, Araujo A, Bauer J, Scarpini C, Coleman N. Tissue transglutaminase mediates the pro-malignant effects of oncostatin M receptor over-expression in cervical squamous cell carcinoma. J Pathol. 2013;231:168-79 pubmed publisher
	Wilken J, Perez Torres M, Nieves Alicea R, Cora E, Christensen T, Baron A, et al. Shedding of soluble epidermal growth factor receptor (sEGFR) is mediated by a metalloprotease/fibronectin/integrin axis and inhibited by cetuximab. Biochemistry. 2013;52:4531-40 pubmed publisher
	Traub S, Morgner J, Martino M, Honing S, Swartz M, Wickström S, et al. The promotion of endothelial cell attachment and spreading using FNIII10 fused to VEGF-A165. Biomaterials. 2013;34:5958-68 pubmed publisher
	Polcz M, Adamson L, Lu X, Chang M, Fowler L, Hobbs J. Increased IL-6 detection in adult and pediatric lymphoid tissue harboring parvovirus B19. J Clin Virol. 2013;57:233-8 pubmed publisher
	Zoppi N, Ritelli M, Colombi M. Type III and V collagens modulate the expression and assembly of EDA(+) fibronectin in the extracellular matrix of defective Ehlers-Danlos syndrome fibroblasts. Biochim Biophys Acta. 2012;1820:1576-87 pubmed publisher
	Tabariès S, Dupuy F, Dong Z, Monast A, Annis M, Spicer J, et al. Claudin-2 promotes breast cancer liver metastasis by facilitating tumor cell interactions with hepatocytes. Mol Cell Biol. 2012;32:2979-91 pubmed publisher
	Popugaeva E, Witkowski P, Schlegel M, Ulrich R, Auste B, Rang A, et al. Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans. PLoS ONE. 2012;7:e35587 pubmed publisher
	Labonté L, Li Y, Addison C, Brand M, Javidnia H, Corsten M, et al. Distinct profile of vascular progenitor attachment to extracellular matrix proteins in cancer patients. Clin Invest Med. 2012;35:E86-95 pubmed
	Gooz P, Dang Y, Higashiyama S, Twal W, Haycraft C, Gooz M. A disintegrin and metalloenzyme (ADAM) 17 activation is regulated by ?5?1 integrin in kidney mesangial cells. PLoS ONE. 2012;7:e33350 pubmed publisher
	Kesanakurti D, Chetty C, Dinh D, Gujrati M, Rao J. Role of MMP-2 in the regulation of IL-6/Stat3 survival signaling via interaction with ?5?1 integrin in glioma. Oncogene. 2013;32:327-40 pubmed publisher
	Liu Z, Li W, Xu H, Huang X, Pan L, Ren Y, et al. In vivo pharmacokinetics, immunogenicity and mechanism of PEGylated antitumor polypeptide. Curr Pharm Des. 2012;18:1655-62 pubmed
	Ogata A, Endo M, Aoi J, Takahashi O, Kadomatsu T, Miyata K, et al. The role of angiopoietin-like protein 2 in pathogenesis of dermatomyositis. Biochem Biophys Res Commun. 2012;418:494-9 pubmed publisher
	Klempa B, Witkowski P, Popugaeva E, Auste B, Koivogui L, Fichet Calvet E, et al. Sangassou virus, the first hantavirus isolate from Africa, displays genetic and functional properties distinct from those of other murinae-associated hantaviruses. J Virol. 2012;86:3819-27 pubmed publisher
	Li Q, Ding X, Thomas J, Harding C, Pecora N, Ziady A, et al. Rv2468c, a novel Mycobacterium tuberculosis protein that costimulates human CD4+ T cells through VLA-5. J Leukoc Biol. 2012;91:311-20 pubmed publisher
	Chen C, Lu D, Yeh M, Shiau C, Chiang C. Novel RGD-lipid conjugate-modified liposomes for enhancing siRNA delivery in human retinal pigment epithelial cells. Int J Nanomedicine. 2011;6:2567-80 pubmed publisher
	Decaris M, Mojadedi A, Bhat A, Leach J. Transferable cell-secreted extracellular matrices enhance osteogenic differentiation. Acta Biomater. 2012;8:744-52 pubmed publisher
	Siegel A, Kimble Hill A, Garg S, Jordan R, Naumann C. Native ligands change integrin sequestering but not oligomerization in raft-mimicking lipid mixtures. Biophys J. 2011;101:1642-50 pubmed publisher
	Hamill K, Hopkinson S, Hoover P, Todorovic V, Green K, Jones J. Fibronectin expression determines skin cell motile behavior. J Invest Dermatol. 2012;132:448-57 pubmed publisher
	Huang R, Teo Z, Chong H, Zhu P, Tan M, Tan C, et al. ANGPTL4 modulates vascular junction integrity by integrin signaling and disruption of intercellular VE-cadherin and claudin-5 clusters. Blood. 2011;118:3990-4002 pubmed publisher
	Caiado F, Carvalho T, Silva F, Castro C, Clode N, Dye J, et al. The role of fibrin E on the modulation of endothelial progenitors adhesion, differentiation and angiogenic growth factor production and the promotion of wound healing. Biomaterials. 2011;32:7096-105 pubmed publisher
	Shannon Lowe C, Rowe M. Epstein-Barr virus infection of polarized epithelial cells via the basolateral surface by memory B cell-mediated transfer infection. PLoS Pathog. 2011;7:e1001338 pubmed publisher
	De Wever O, Sobczak Thepot J, Vercoutter Edouart A, Michalski J, Ouelaa Benslama R, Stupack D, et al. Priming and potentiation of DNA damage response by fibronectin in human colon cancer cells and tumor-derived myofibroblasts. Int J Oncol. 2011;39:393-400 pubmed publisher
	Li Z, Kreiner M, van der Walle C, Mardon H. Clustered integrin ?5?1 ligand displays model fibronectin-mediated adhesion of human endometrial stromal cells. Biochem Biophys Res Commun. 2011;407:777-82 pubmed publisher
	Fong Y, Lin C, Su Y, Chen W, Tsai F, Tsai C, et al. CCN6 enhances ICAM-1 expression and cell motility in human chondrosarcoma cells. J Cell Physiol. 2012;227:223-32 pubmed publisher
	Chuang J, Yang W, Lai C, Lin C, Tsai M, Tang C. CTGF inhibits cell motility and COX-2 expression in oral cancer cells. Int Immunopharmacol. 2011;11:948-54 pubmed publisher
	Kato T, Bilim V, Yuuki K, Naito S, Yamanobe T, Nagaoka A, et al. Bacillus Calmette-Guerin and BCG cell wall skeleton suppressed viability of bladder cancer cells in vitro. Anticancer Res. 2010;30:4089-96 pubmed
	Attia M, Santerre J, Kandel R. The response of annulus fibrosus cell to fibronectin-coated nanofibrous polyurethane-anionic dihydroxyoligomer scaffolds. Biomaterials. 2011;32:450-60 pubmed publisher
	Coulson Thomas V, Gesteira T, Coulson Thomas Y, Vicente C, Tersariol I, Nader H, et al. Fibroblast and prostate tumor cell cross-talk: fibroblast differentiation, TGF-?, and extracellular matrix down-regulation. Exp Cell Res. 2010;316:3207-26 pubmed publisher
	Christensen B, Schack L, Kläning E, Sørensen E. Osteopontin is cleaved at multiple sites close to its integrin-binding motifs in milk and is a novel substrate for plasmin and cathepsin D. J Biol Chem. 2010;285:7929-37 pubmed publisher
	Zahn G, Volk K, Lewis G, Vossmeyer D, Stragies R, Heier J, et al. Assessment of the integrin alpha5beta1 antagonist JSM6427 in proliferative vitreoretinopathy using in vitro assays and a rabbit model of retinal detachment. Invest Ophthalmol Vis Sci. 2010;51:1028-35 pubmed publisher
	Kim H, Kim P, Bae S, Son H, Thoudam D, Kim J, et al. Transforming growth factor-beta-induced protein (TGFBIp/beta ig-h3) activates platelets and promotes thrombogenesis. Blood. 2009;114:5206-15 pubmed publisher
	Tandle A, Calvani M, Uranchimeg B, Zahavi D, Melillo G, Libutti S. Endothelial monocyte activating polypeptide-II modulates endothelial cell responses by degrading hypoxia-inducible factor-1alpha through interaction with PSMA7, a component of the proteasome. Exp Cell Res. 2009;315:1850-9 pubmed publisher
	Le Maitre C, Frain J, Millward Sadler J, Fotheringham A, Freemont A, Hoyland J. Altered integrin mechanotransduction in human nucleus pulposus cells derived from degenerated discs. Arthritis Rheum. 2009;60:460-9 pubmed publisher
	Pi L, Ding X, Jorgensen M, Pan J, Oh S, Pintilie D, et al. Connective tissue growth factor with a novel fibronectin binding site promotes cell adhesion and migration during rat oval cell activation. Hepatology. 2008;47:996-1004 pubmed publisher
	Wu L, Bernard Trifilo J, Lim Y, Lim S, Mitra S, Uryu S, et al. Distinct FAK-Src activation events promote alpha5beta1 and alpha4beta1 integrin-stimulated neuroblastoma cell motility. Oncogene. 2008;27:1439-48 pubmed
	Diaz L, Chuan Y, Lewitt M, Fernandez Perez L, Carrasco Rodríguez S, Sanchez Gomez M, et al. IGF-II regulates metastatic properties of choriocarcinoma cells through the activation of the insulin receptor. Mol Hum Reprod. 2007;13:567-76 pubmed
	Larsen C, Kligman F, Tang C, Kottke Marchant K, Marchant R. A biomimetic peptide fluorosurfactant polymer for endothelialization of ePTFE with limited platelet adhesion. Biomaterials. 2007;28:3537-48 pubmed
	McNally A, Macewan S, Anderson J. alpha subunit partners to beta1 and beta2 integrins during IL-4-induced foreign body giant cell formation. J Biomed Mater Res A. 2007;82:568-74 pubmed
	Huang Y, Wu C, Chen M, Fung C, Peng H. Characterization of the type 3 fimbriae with different MrkD adhesins: possible role of the MrkD containing an RGD motif. Biochem Biophys Res Commun. 2006;350:537-42 pubmed
	Riemenschneider M, Mueller W, Betensky R, Mohapatra G, Louis D. In situ analysis of integrin and growth factor receptor signaling pathways in human glioblastomas suggests overlapping relationships with focal adhesion kinase activation. Am J Pathol. 2005;167:1379-87 pubmed
	Bix G, Fu J, Gonzalez E, Macro L, Barker A, Campbell S, et al. Endorepellin causes endothelial cell disassembly of actin cytoskeleton and focal adhesions through alpha2beta1 integrin. J Cell Biol. 2004;166:97-109 pubmed

product information

Catalog Number :

MAB1969

Subcategory :

Cell Structure

Product Name :

Anti-Integrin α5β1 Antibody, clone JBS5

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P08648

Host Name :

Mouse

Antigen :

Integrin α5β1

Clone :

JBS5

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-Integrin α5β1 Antibody, clone JBS5

Cross Reactivity :

Human;Monkey

Background :

Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.

ALT Names :

VLA-5

Immunogen :

Jurkat Cells

Specificity :

Reacts with the human integrin alpha5beta1.

Package Size :

100 μL

Uses :

Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Affects Function

Storage :

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.