Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10) | EMD Millipore MAB1965 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10)

catalog :

MAB1965

quantity :

100 μL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

JB1A

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments, other

citations: 37

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; loading ...; fig 4b	Oyama M, Kariya Y, Kariya Y, Matsumoto K, Kanno M, Yamaguchi Y, et al. Biological role of site-specific O-glycosylation in cell adhesion activity and phosphorylation of osteopontin. Biochem J. 2018;475:1583-1595 pubmed publisher
immunoprecipitation; human	Yalcin E, He Y, Orhan D, Pazzagli C, Emiralioğlu N, Has C. Crucial role of posttranslational modifications of integrin Î±3 in interstitial lung disease and nephrotic syndrome. Hum Mol Genet. 2015;24:3679-88 pubmed publisher
other; mouse; 1:250; loading ...; fig 2a	Woo J, Zhao X, Khan H, Penn C, Wang X, Joly Amado A, et al. Slingshot-Cofilin activation mediates mitochondrial and synaptic dysfunction via AÎ² ligation to Î²1-integrin conformers. Cell Death Differ. 2015;22:921-34 pubmed publisher
other; human; loading ...; fig 5A western blot; human; loading ...; fig 6C	Lee H, Kim Y, Kim D. Exosomes derived from human macrophages suppress endothelial cell migration by controlling integrin trafficking. Eur J Immunol. 2014;44:1156-69 pubmed publisher
immunohistochemistry; mouse	Barbaro V, Ferrari S, Fasolo A, Ponzin D, Di Iorio E. Reconstruction of a human hemicornea through natural scaffolds compatible with the growth of corneal epithelial stem cells and stromal keratocytes. Mol Vis. 2009;15:2084-93 pubmed
	Li L, Jiang H, Xie Y, Liao L, Cao H, Xu X, et al. Macrolide analog F806 suppresses esophageal squamous cell carcinoma (ESCC) by blocking β1 integrin activation. Oncotarget. 2015;6:15940-52 pubmed
	Klingberg F, Chow M, Koehler A, Boo S, Buscemi L, Quinn T, et al. Prestress in the extracellular matrix sensitizes latent TGF-Î²1 for activation. J Cell Biol. 2014;207:283-97 pubmed publisher
	Ho M, Hung S, Liang C, Liang S. Recombinant viral capsid protein VP1 suppresses lung cancer metastasis by inhibiting COX-2/PGE2 and MIG-7. Oncotarget. 2014;5:3931-43 pubmed
	Hashiba K, Sano M, Nio Kobayashi J, Hojo T, Skarzynski D, Okuda K. Galectin-3 contributes to luteolysis by binding to Beta 1 integrin in the bovine corpus luteum. Biol Reprod. 2014;91:2 pubmed publisher
	Nadalutti C, Korponay Szabo I, Kaukinen K, Griffin M, Maki M, Lindfors K. Celiac disease patient IgA antibodies induce endothelial adhesion and cell polarization defects via extracellular transglutaminase 2. Cell Mol Life Sci. 2014;71:1315-26 pubmed publisher
	Carduner L, Agniel R, Kellouche S, Picot C, Blanc Fournier C, Leroy Dudal J, et al. Ovarian cancer ascites-derived vitronectin and fibronectin: combined purification, molecular features and effects on cell response. Biochim Biophys Acta. 2013;1830:4885-97 pubmed publisher
	Tong L, Png E, Aihua H, Yong S, Yeo H, Riau A, et al. Molecular mechanism of transglutaminase-2 in corneal epithelial migration and adhesion. Biochim Biophys Acta. 2013;1833:1304-15 pubmed publisher
	Kumar P, Kumar P, Lindberg L, Thirkill T, Ji J, Martsching L, et al. The MUC1 extracellular domain subunit is found in nuclear speckles and associates with spliceosomes. PLoS ONE. 2012;7:e42712 pubmed publisher
	Tong W, Shen W, Yeung C, Zhao Y, Cheng S, Chu P, et al. Functional replication of the tendon tissue microenvironment by a bioimprinted substrate and the support of tenocytic differentiation of mesenchymal stem cells. Biomaterials. 2012;33:7686-98 pubmed publisher
	Chiu C, Peng J, Hung S, Liang C, Liang S. Recombinant viral capsid protein VP1 suppresses migration and invasion of human cervical cancer by modulating phosphorylated prohibitin in lipid rafts. Cancer Lett. 2012;320:205-14 pubmed publisher
	Peng J, Chen Y, Hung S, Chiu C, Ho M, Lee Y, et al. Recombinant viral protein promotes apoptosis and suppresses invasion of ovarian adenocarcinoma cells by targeting ?5?1 integrin to down-regulate Akt and MMP-2. Br J Pharmacol. 2012;165:479-93 pubmed publisher
	Suen R, Rampersad S, Stewart D, Courtman D. Differential roles of endothelin-1 in angiotensin II-induced atherosclerosis and aortic aneurysms in apolipoprotein E-null mice. Am J Pathol. 2011;179:1549-59 pubmed publisher
	Wright S, Parham C, Lee B, Clarke D, Auckland L, Johnston J, et al. Perlecan domain V inhibits ?2 integrin-mediated amyloid-? neurotoxicity. Neurobiol Aging. 2012;33:1379-88 pubmed publisher
	Garamszegi N, Garamszegi S, Samavarchi Tehrani P, Walford E, Schneiderbauer M, Wrana J, et al. Extracellular matrix-induced transforming growth factor-beta receptor signaling dynamics. Oncogene. 2010;29:2368-80 pubmed publisher
	Kaomongkolgit R, Manokawinchoke J, Sanchavanakit N, Pavasant P, Sumrejkanchanakij P. Fibronectin supports TNF-alpha-induced osteopontin expression through beta1 integrin and ERK in HN-22 cells. Arch Oral Biol. 2010;55:101-7 pubmed publisher
	Peng L, Xing X, Li W, Qu L, Meng L, Lian S, et al. PRL-3 promotes the motility, invasion, and metastasis of LoVo colon cancer cells through PRL-3-integrin beta1-ERK1/2 and-MMP2 signaling. Mol Cancer. 2009;8:110 pubmed publisher
	Iwashita J, Yamamoto T, Sasaki Y, Abe T. MUC5AC production is downregulated in NCI-H292 lung cancer cells cultured on type-IV collagen. Mol Cell Biochem. 2010;337:65-75 pubmed publisher
	Chai D, Arner E, Griggs D, Grodzinsky A. Alphav and beta1 integrins regulate dynamic compression-induced proteoglycan synthesis in 3D gel culture by distinct complementary pathways. Osteoarthritis Cartilage. 2010;18:249-56 pubmed publisher
	Isaji T, Sato Y, Fukuda T, Gu J. N-glycosylation of the I-like domain of beta1 integrin is essential for beta1 integrin expression and biological function: identification of the minimal N-glycosylation requirement for alpha5beta1. J Biol Chem. 2009;284:12207-16 pubmed publisher
	Le Maitre C, Frain J, Millward Sadler J, Fotheringham A, Freemont A, Hoyland J. Altered integrin mechanotransduction in human nucleus pulposus cells derived from degenerated discs. Arthritis Rheum. 2009;60:460-9 pubmed publisher
	Zhuo Y, Chammas R, Bellis S. Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis. J Biol Chem. 2008;283:22177-85 pubmed publisher
	Kirkland S, Ying H. Alpha2beta1 integrin regulates lineage commitment in multipotent human colorectal cancer cells. J Biol Chem. 2008;283:27612-9 pubmed publisher
	Ghosh D, Beavis R, Wilkins J. The identification and characterization of membranome components. J Proteome Res. 2008;7:1572-83 pubmed publisher
	Lim J, Dreiss A, Zhou Z, Hansen J, Siedlecki C, Hengstebeck R, et al. The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography. Biomaterials. 2007;28:1787-97 pubmed
	Yasuda M, Miyachi Y, Ishikawa O, Takahashi K. Spatial expressions of fibronectin and integrins by human and rodent dermal fibroblasts. Br J Dermatol. 2006;155:522-31 pubmed
	Koenig A, Mueller C, Hasel C, Adler G, Menke A. Collagen type I induces disruption of E-cadherin-mediated cell-cell contacts and promotes proliferation of pancreatic carcinoma cells. Cancer Res. 2006;66:4662-71 pubmed
	Kierszenbaum A, Rosselot C, Rivkin E, Tres L. Role of integrins, tetraspanins, and ADAM proteins during the development of apoptotic bodies by spermatogenic cells. Mol Reprod Dev. 2006;73:906-17 pubmed
	Wright S, Malinin N, Powell K, Yednock T, Rydel R, Griswold Prenner I. Alpha2beta1 and alphaVbeta1 integrin signaling pathways mediate amyloid-beta-induced neurotoxicity. Neurobiol Aging. 2007;28:226-37 pubmed
	Meng X, Wilkins J. Compositional characterization of the cytoskeleton of NK-like cells. J Proteome Res. 2005;4:2081-7 pubmed
	Seales E, Shaikh F, Woodard Grice A, Aggarwal P, McBrayer A, Hennessy K, et al. A protein kinase C/Ras/ERK signaling pathway activates myeloid fibronectin receptors by altering beta1 integrin sialylation. J Biol Chem. 2005;280:37610-5 pubmed
	Lim J, Taylor A, Li Z, Vogler E, Donahue H. Integrin expression and osteopontin regulation in human fetal osteoblastic cells mediated by substratum surface characteristics. Tissue Eng. 2005;11:19-29 pubmed
	Akimov S, Krylov D, Fleischman L, Belkin A. Tissue transglutaminase is an integrin-binding adhesion coreceptor for fibronectin. J Cell Biol. 2000;148:825-38 pubmed

product information

Catalog Number :

MAB1965

Subcategory :

Cell Structure

Product Name :

Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10)

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P05556

Host Name :

Mouse

Antigen :

Integrin β1

Clone :

JB1A (a.k.a. J10)

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10)

Cross Reactivity :

Human

Background :

The primary function of the integrin family is to mediate cell-cell and cell-matrix adhesion interaction. CD29 is ubiquitous, with broad tissue distribution, but is not expressed on erythrocytes and is expressed only weakly on granulocytes. Integrins play an important role in cell adhesion and migration, and their normal function is critical in the induction and maintenance of cell differentiation. Loss or downregulation of CD29 has been proposed to be one of the general pathways through which carcinoma cells may acquire a more invasive and differentiated phenotype. Furthermore, loss or downregulation of integrin occurs relatively frequently in poorly differentiated colorectal and breast carcinomas as determined by immunohistochemistry.

ALT Names :

CD29

Immunogen :

Purified beta 1 integrin and Jurkat cells

Specificity :

Reacts with the human integrin beta 1. Note: integrin beta 1 is expressed in all tissues/cell types except erythrocytes. The epitope recognized by JB1a was recently mapped to residues 82-87 of the mature beta 1 integrin chain. In this study Mn++ was found to inhibit binding of JB1a both to mature beta 1 integrin and to a synthetic peptide corresponding to residues 82-87{Ni, 1998}.

Package Size :

100 μL

Uses :

Flow Cytometry;Immunohistochemistry;Immunoprecipitation;Affects Function;Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.