This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Integrin αVβ5 Antibody, clone P1F6
catalog :
MAB1961
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
P1F6

The same clone is also sold as:
reactivity :
human, rat, chicken
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments
citations: 73
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; rat; 1:125; loading ...; fig 2d
  • western blot; rat; loading ...; fig 2e, 2f
Werkman I, Sikkema A, Versluijs J, Qin J, de Boer P, Baron W. TLR3 agonists induce fibronectin aggregation by activated astrocytes: a role of pro-inflammatory cytokines and fibronectin splice variants. Sci Rep. 2020;10:532 pubmed publisher
  • flow cytometry; human; loading ...; fig 1
Nestić D, Uil T, Ma J, Roy S, Vellinga J, Baker A, et al. αvβ3 Integrin Is Required for Efficient Infection of Epithelial Cells with Human Adenovirus Type 26. J Virol. 2019;93: pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 6d
Gopal S, Veracini L, Grall D, Butori C, Schaub S, Audebert S, et al. Fibronectin-guided migration of carcinoma collectives. Nat Commun. 2017;8:14105 pubmed publisher
  • flow cytometry; human; tbl 3
Kaliberov S, Kaliberova L, Yan H, Kapoor V, Hallahan D. Retargeted adenoviruses for radiation-guided gene delivery. Cancer Gene Ther. 2016;23:303-14 pubmed publisher
  • flow cytometry; human; 1:200; loading ...; fig 1d
Gvozdenović A, Boro A, Meier D, Bode Lesniewska B, Born W, Muff R, et al. Targeting αvβ3 and αvβ5 integrins inhibits pulmonary metastasis in an intratibial xenograft osteosarcoma mouse model. Oncotarget. 2016;7:55141-55154 pubmed publisher
  • flow cytometry; human
Krishnan S, Szabo E, Burghardt I, Frei K, Tabatabai G, Weller M. Modulation of cerebral endothelial cell function by TGF-β in glioblastoma: VEGF-dependent angiogenesis versus endothelial mesenchymal transition. Oncotarget. 2015;6:22480-95 pubmed
  • flow cytometry; human; fig 1
Sugahara K, Braun G, de Mendoza T, Kotamraju V, French R, Lowy A, et al. Tumor-penetrating iRGD peptide inhibits metastasis. Mol Cancer Ther. 2015;14:120-8 pubmed publisher
  • flow cytometry; human
  • immunocytochemistry; human
Horiguchi H, Endo M, Miyamoto Y, Sakamoto Y, Odagiri H, Masuda T, et al. Angiopoietin-like protein 2 renders colorectal cancer cells resistant to chemotherapy by activating spleen tyrosine kinase-phosphoinositide 3-kinase-dependent anti-apoptotic signaling. Cancer Sci. 2014;105:1550-9 pubmed publisher
  • blocking or activating experiments; human
Wei Y, Zhang Y, Cai H, Mirza A, Iorio R, Peeples M, et al. Roles of the putative integrin-binding motif of the human metapneumovirus fusion (f) protein in cell-cell fusion, viral infectivity, and pathogenesis. J Virol. 2014;88:4338-52 pubmed publisher
  • flow cytometry; human
Sallach J, Di Pasquale G, Larcher F, Niehoff N, Rübsam M, Huber A, et al. Tropism-modified AAV vectors overcome barriers to successful cutaneous therapy. Mol Ther. 2014;22:929-39 pubmed publisher
  • flow cytometry; human
Majhen D, Stojanović N, Vukić D, Pichon C, Leduc C, Osmak M, et al. Increased adenovirus Type 5 mediated transgene expression due to RhoB down-regulation. PLoS ONE. 2014;9:e86698 pubmed publisher
  • western blot; human
Bianchi Smiraglia A, Kunnev D, Limoge M, Lee A, Beckerle M, Bakin A. Integrin-?5 and zyxin mediate formation of ventral stress fibers in response to transforming growth factor ?. Cell Cycle. 2013;12:3377-89 pubmed publisher
  • immunocytochemistry; human; 1:100
Chen C, Yeh M, Shiau C, Chiang C, Lu D. Efficient downregulation of VEGF in retinal pigment epithelial cells by integrin ligand-labeled liposome-mediated siRNA delivery. Int J Nanomedicine. 2013;8:2613-27 pubmed publisher
  • blocking or activating experiments; human; 20 ug/ml
Azab W, Lehmann M, Osterrieder N. Glycoprotein H and ?4?1 integrins determine the entry pathway of alphaherpesviruses. J Virol. 2013;87:5937-48 pubmed publisher
  • blocking or activating experiments; human
Cabarcas S, Sun L, Mathews L, Thomas S, Zhang X, Farrar W. The differentiation of pancreatic tumor-initiating cells by vitronectin can be blocked by cilengitide. Pancreas. 2013;42:861-70 pubmed publisher
Klingberg F, Chow M, Koehler A, Boo S, Buscemi L, Quinn T, et al. Prestress in the extracellular matrix sensitizes latent TGF-β1 for activation. J Cell Biol. 2014;207:283-97 pubmed publisher
Li X, Liu Y, Haas T. Peptides derived from central turn motifs within integrin αIIb and αV cytoplasmic tails inhibit integrin activation. Peptides. 2014;62:38-48 pubmed publisher
Jiang X, Teng M, Guo X, Zhang D, Zhang Q, Zhang J, et al. Switch from αvβ5 to αvβ6 integrin is required for CD9-regulated keratinocyte migration and MMP-9 activation. FEBS Lett. 2014;588:4044-52 pubmed publisher
Hsiao C, Wang W, Kuo W, Chen H, Chen T, Hamann J, et al. CD97 inhibits cell migration in human fibrosarcoma cells by modulating TIMP-2/MT1- MMP/MMP-2 activity--role of GPS autoproteolysis and functional cooperation between the N- and C-terminal fragments. FEBS J. 2014;281:4878-91 pubmed publisher
Zhan Y, Yu B, Wang Z, Zhang Y, Zhang H, Wu H, et al. A fiber-modified adenovirus co-expressing HSV-TK and Coli.NTR enhances antitumor activities in breast cancer cells. Int J Clin Exp Pathol. 2014;7:2850-60 pubmed
Kim B, Jang I, Shin S, Kwon Y, Heo S, Choi E, et al. Therapeutic angiogenesis in a murine model of limb ischemia by recombinant periostin and its fasciclin I domain. Biochim Biophys Acta. 2014;1842:1324-32 pubmed publisher
Ray A, Schaffner F, Janouskova H, Noulet F, Rognan D, Lelong Rebel I, et al. Single cell tracking assay reveals an opposite effect of selective small non-peptidic ?5?1 or ?v?3/?5 integrin antagonists in U87MG glioma cells. Biochim Biophys Acta. 2014;1840:2978-87 pubmed publisher
Rapraeger A, Ell B, Roy M, Li X, Morrison O, Thomas G, et al. Vascular endothelial-cadherin stimulates syndecan-1-coupled insulin-like growth factor-1 receptor and cross-talk between ?V?3 integrin and vascular endothelial growth factor receptor 2 at the onset of endothelial cell dissemination during angiogenesi. FEBS J. 2013;280:2194-206 pubmed publisher
Majhen D, Richardson J, Vukelić B, Dodig I, Cindric M, Benihoud K, et al. The disulfide bond of an RGD4C motif inserted within the Hi loop of the adenovirus type 5 fiber protein is critical for retargeting to ?v -integrins. J Gene Med. 2012;14:788-97 pubmed publisher
Canonici A, Pellegrino E, Siret C, Terciolo C, Czerucka D, Bastonero S, et al. Saccharomyces boulardii improves intestinal epithelial cell restitution by inhibiting ?v?5 integrin activation state. PLoS ONE. 2012;7:e45047 pubmed publisher
Westenskow P, Moreno S, Krohne T, Kurihara T, Zhu S, Zhang Z, et al. Using flow cytometry to compare the dynamics of photoreceptor outer segment phagocytosis in iPS-derived RPE cells. Invest Ophthalmol Vis Sci. 2012;53:6282-90 pubmed publisher
Bianchi Smiraglia A, Paesante S, Bakin A. Integrin ?5 contributes to the tumorigenic potential of breast cancer cells through the Src-FAK and MEK-ERK signaling pathways. Oncogene. 2013;32:3049-58 pubmed publisher
Jandova J, Beyer T, Meuillet E, Watts G. The matrix protein CCN1/CYR61 is required for ?(V)?5-mediated cancer cell migration. Cell Biochem Funct. 2012;30:687-95 pubmed publisher
Kim S, Park S, Kim S, Bae D, Pyo J, Hong M, et al. Cross talk between engulfment receptors stabilin-2 and integrin ?v?5 orchestrates engulfment of phosphatidylserine-exposed erythrocytes. Mol Cell Biol. 2012;32:2698-708 pubmed publisher
Hung W, Huang K, Wu C, Chi C, Kao H, Li A, et al. Mitochondrial dysfunction promotes cell migration via reactive oxygen species-enhanced ?5-integrin expression in human gastric cancer SC-M1 cells. Biochim Biophys Acta. 2012;1820:1102-10 pubmed publisher
Bzowska M, Nogieć A, Skrzeczyńska Moncznik J, Mickowska B, Guzik K, Pryjma J. Oxidized LDLs inhibit TLR-induced IL-10 production by monocytes: a new aspect of pathogen-accelerated atherosclerosis. Inflammation. 2012;35:1567-84 pubmed publisher
Edkins A, Borland G, Acharya M, Cogdell R, Ozanne B, Cushley W. Differential regulation of monocyte cytokine release by ?V and ?(2) integrins that bind CD23. Immunology. 2012;136:241-51 pubmed publisher
Haseley A, Boone S, Wojton J, Yu L, Yoo J, Yu J, et al. Extracellular matrix protein CCN1 limits oncolytic efficacy in glioma. Cancer Res. 2012;72:1353-62 pubmed publisher
Azab W, Osterrieder N. Glycoproteins D of equine herpesvirus type 1 (EHV-1) and EHV-4 determine cellular tropism independently of integrins. J Virol. 2012;86:2031-44 pubmed publisher
Qin S, Rodrigues G. Roles of ?v?5, FAK and MerTK in oxidative stress inhibition of RPE cell phagocytosis. Exp Eye Res. 2012;94:63-70 pubmed publisher
Borovjagin A, Dong J, Passineau M, Ren C, Lamani E, Mamaeva O, et al. Adenovirus gene transfer to amelogenesis imperfecta ameloblast-like cells. PLoS ONE. 2011;6:e24281 pubmed publisher
Huang C, Zhang J, Ao M, Li Y, Zhang C, Xu Y, et al. Combination of retinal pigment epithelium cell-conditioned medium and photoreceptor outer segments stimulate mesenchymal stem cell differentiation toward a functional retinal pigment epithelium cell phenotype. J Cell Biochem. 2012;113:590-8 pubmed publisher
Conradi J, Huber S, Gaus K, Mertink F, Royo Gracia S, Strijowski U, et al. Cyclic RGD peptides interfere with binding of the Helicobacter pylori protein CagL to integrins ?V?3 and ?5?1. Amino Acids. 2012;43:219-32 pubmed publisher
Majhen D, Stojanović N, Špeljko T, Brozovic A, De Zan T, Osmak M, et al. Increased expression of the coxsackie and adenovirus receptor downregulates ?v?3 and ?v?5 integrin expression and reduces cell adhesion and migration. Life Sci. 2011;89:241-9 pubmed publisher
Shannon Lowe C, Rowe M. Epstein-Barr virus infection of polarized epithelial cells via the basolateral surface by memory B cell-mediated transfer infection. PLoS Pathog. 2011;7:e1001338 pubmed publisher
Zhang Y, Chen M, Venugopal S, Zhou Y, Xiang W, Li Y, et al. Isthmin exerts pro-survival and death-promoting effect on endothelial cells through alphavbeta5 integrin depending on its physical state. Cell Death Dis. 2011;2:e153 pubmed publisher
Kim J, Nam H, Kim T, Kim P, Ryu J, Yun C, et al. Active targeting of RGD-conjugated bioreducible polymer for delivery of oncolytic adenovirus expressing shRNA against IL-8 mRNA. Biomaterials. 2011;32:5158-66 pubmed publisher
Grzesiak J, Tran Cao H, Burton D, Kaushal S, Vargas F, Clopton P, et al. Knockdown of the ?(1) integrin subunit reduces primary tumor growth and inhibits pancreatic cancer metastasis. Int J Cancer. 2011;129:2905-15 pubmed publisher
Silver J, Mei Y. Transduction and oncolytic profile of a potent replication-competent adenovirus 11p vector (RCAd11pGFP) in colon carcinoma cells. PLoS ONE. 2011;6:e17532 pubmed publisher
Fukui N, Ikeda Y, Tanaka N, Wake M, Yamaguchi T, Mitomi H, et al. ?v?5 integrin promotes dedifferentiation of monolayer-cultured articular chondrocytes. Arthritis Rheum. 2011;63:1938-49 pubmed publisher
Desai R, Khan M, Gopal S, Chen C. Subcellular spatial segregation of integrin subtypes by patterned multicomponent surfaces. Integr Biol (Camb). 2011;3:560-7 pubmed publisher
Hsiao C, Chen H, Chang G, Lin H. GPS autoproteolysis is required for CD97 to up-regulate the expression of N-cadherin that promotes homotypic cell-cell aggregation. FEBS Lett. 2011;585:313-8 pubmed publisher
de Vrij J, Van Den Hengel S, Uil T, Koppers Lalic D, Dautzenberg I, Stassen O, et al. Enhanced transduction of CAR-negative cells by protein IX-gene deleted adenovirus 5 vectors. Virology. 2011;410:192-200 pubmed publisher
Reinehr R, Sommerfeld A, Haussinger D. Insulin induces swelling-dependent activation of the epidermal growth factor receptor in rat liver. J Biol Chem. 2010;285:25904-12 pubmed publisher
Okada T, Tsukano H, Endo M, Tabata M, Miyata K, Kadomatsu T, et al. Synoviocyte-derived angiopoietin-like protein 2 contributes to synovial chronic inflammation in rheumatoid arthritis. Am J Pathol. 2010;176:2309-19 pubmed publisher
Hedlund S, Persson A, Vujic A, Che K, Stendahl O, Larsson M. Dendritic cell activation by sensing Mycobacterium tuberculosis-induced apoptotic neutrophils via DC-SIGN. Hum Immunol. 2010;71:535-40 pubmed publisher
Kessentini Zouari R, Jebali J, Taboubi S, Srairi Abid N, Morjen M, Kallech Ziri O, et al. CC-PLA2-1 and CC-PLA2-2, two Cerastes cerastes venom-derived phospholipases A2, inhibit angiogenesis both in vitro and in vivo. Lab Invest. 2010;90:510-9 pubmed publisher
Cui J, Maberley D, Samad A, Ma P, Ning A, Matsubara J, et al. Expression of integrins on human choroidal neovascular membranes. J Ocul Biol Dis Infor. 2009;2:12-9 pubmed publisher
Dummula K, Vinukonda G, Xu H, Hu F, Zia M, Braun A, et al. Development of integrins in the vasculature of germinal matrix, cerebral cortex, and white matter of fetuses and premature infants. J Neurosci Res. 2010;88:1193-204 pubmed publisher
Rowland T, Miller L, Blaschke A, Doss E, Bonham A, Hikita S, et al. Roles of integrins in human induced pluripotent stem cell growth on Matrigel and vitronectin. Stem Cells Dev. 2010;19:1231-40 pubmed publisher
Supino R, Scovassi A, Croce A, Dal Bo L, Favini E, Corbelli A, et al. Biological effects of a new vacuolar-H,-ATPase inhibitor in colon carcinoma cell lines. Ann N Y Acad Sci. 2009;1171:606-16 pubmed publisher
Shelton L, Rada J. Inhibition of human scleral fibroblast cell attachment to collagen type I by TGFBIp. Invest Ophthalmol Vis Sci. 2009;50:3542-52 pubmed publisher
Juric V, Chen C, Lau L. Fas-mediated apoptosis is regulated by the extracellular matrix protein CCN1 (CYR61) in vitro and in vivo. Mol Cell Biol. 2009;29:3266-79 pubmed publisher
Beauvais D, Ell B, McWhorter A, Rapraeger A. Syndecan-1 regulates alphavbeta3 and alphavbeta5 integrin activation during angiogenesis and is blocked by synstatin, a novel peptide inhibitor. J Exp Med. 2009;206:691-705 pubmed publisher
Sandberg L, Papareddy P, Silver J, Bergh A, Mei Y. Replication-competent Ad11p vector (RCAd11p) efficiently transduces and replicates in hormone-refractory metastatic prostate cancer cells. Hum Gene Ther. 2009;20:361-73 pubmed publisher
Schmid M, Varner J. Chapter 15. Methods to study myeloid cell roles in angiogenesis. Methods Enzymol. 2008;445:343-71 pubmed publisher
Holledge M, Millward Sadler S, Nuki G, Salter D. Mechanical regulation of proteoglycan synthesis in normal and osteoarthritic human articular chondrocytes--roles for alpha5 and alphaVbeta5 integrins. Biorheology. 2008;45:275-88 pubmed
Ma C, Rong Y, Radiloff D, Datto M, Centeno B, Bao S, et al. Extracellular matrix protein betaig-h3/TGFBI promotes metastasis of colon cancer by enhancing cell extravasation. Genes Dev. 2008;22:308-21 pubmed publisher
Fukushima Y, Tamura M, Nakagawa H, Itoh K. Induction of glioma cell migration by vitronectin in human serum and cerebrospinal fluid. J Neurosurg. 2007;107:578-85 pubmed
Hakkarainen T, Sarkioja M, Lehenkari P, Miettinen S, Ylikomi T, Suuronen R, et al. Human mesenchymal stem cells lack tumor tropism but enhance the antitumor activity of oncolytic adenoviruses in orthotopic lung and breast tumors. Hum Gene Ther. 2007;18:627-41 pubmed
Martina Y, Avitabile D, Piersanti S, Cherubini G, Saggio I. Different modulation of cellular transcription by adenovirus 5, DeltaE1/E3 adenovirus and helper-dependent vectors. Virus Res. 2007;130:71-84 pubmed
Elayadi A, Samli K, Prudkin L, Liu Y, Bian A, Xie X, et al. A peptide selected by biopanning identifies the integrin alphavbeta6 as a prognostic biomarker for nonsmall cell lung cancer. Cancer Res. 2007;67:5889-95 pubmed
Tanaka T, Huang J, Hirai S, Kuroki M, Kuroki M, Watanabe N, et al. Carcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin Cancer Res. 2006;12:3803-13 pubmed
Wright S, Malinin N, Powell K, Yednock T, Rydel R, Griswold Prenner I. Alpha2beta1 and alphaVbeta1 integrin signaling pathways mediate amyloid-beta-induced neurotoxicity. Neurobiol Aging. 2007;28:226-37 pubmed
Pande P, Mosleh T, Aust A. Role of alphavbeta5 integrin receptor in endocytosis of crocidolite and its effect on intracellular glutathione levels in human lung epithelial (A549) cells. Toxicol Appl Pharmacol. 2006;210:70-7 pubmed
Sun X, Wei L, Liden J, Hui G, Dahlman Wright K, Hjerpe A, et al. Molecular characterization of tumour heterogeneity and malignant mesothelioma cell differentiation by gene profiling. J Pathol. 2005;207:91-101 pubmed
Wang T, Ward Y, Tian L, Lake R, Guedez L, Stetler Stevenson W, et al. CD97, an adhesion receptor on inflammatory cells, stimulates angiogenesis through binding integrin counterreceptors on endothelial cells. Blood. 2005;105:2836-44 pubmed
Hamidi S, Salo T, Kainulainen T, Epstein J, Lerner K, Larjava H. Expression of alpha(v)beta6 integrin in oral leukoplakia. Br J Cancer. 2000;82:1433-40 pubmed
product information
Catalog Number :
MAB1961
Subcategory :
Cell Structure
Product Name :
Anti-Integrin αVβ5 Antibody, clone P1F6
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P06756
Host Name :
Mouse
Antigen :
Integrin αVβ5
Clone :
P1F6
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-Integrin αVβ5 Antibody, clone P1F6
Cross Reactivity :
Chicken;Human
Background :
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha V chain. Alpha chain V undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Immunogen :
Human UCLA P3 cells (lung carcinoma)
Specificity :
Reacts with the human integrin alphaVbeta5.
Package Size :
100 μg
Uses :
Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Affects Function
Storage :
Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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