Integrin β1 Antibody, clone P5D2 | EMD Millipore MAB1959 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Integrin β1 Antibody, clone P5D2

catalog :

MAB1959

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P5D2

The same clone is also sold as:

BioLegend: 921303, 921304
Abcam: ab24693
R&D Systems: FAB17781P, MAB17781
Developmental Studies Hybridoma Bank: P5D2
Santa Cruz Biotechnology: sc-13590 L, sc-13590

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

citations: 49

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; fig 4	HÃ¸ye A, Couchman J, Wewer U, Yoneda A. The Phosphorylation and Distribution of Cortactin Downstream of Integrin Î±9Î²1 Affects Cancer Cell Behaviour. Sci Rep. 2016;6:28529 pubmed publisher
blocking or activating experiments; human	Wei Y, Zhang Y, Cai H, Mirza A, Iorio R, Peeples M, et al. Roles of the putative integrin-binding motif of the human metapneumovirus fusion (f) protein in cell-cell fusion, viral infectivity, and pathogenesis. J Virol. 2014;88:4338-52 pubmed publisher
immunocytochemistry; human; 1:500	Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M. Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop. BMC Cancer. 2013;13:154 pubmed publisher
immunocytochemistry; human	Strauss S, Neumeister A, Barcikowski S, Kracht D, Kuhbier J, Radtke C, et al. Adhesion, vitality and osteogenic differentiation capacity of adipose derived stem cells seeded on nitinol nanoparticle coatings. PLoS ONE. 2013;8:e53309 pubmed publisher
	Condello S, Sima L, Ivan C, Cárdenas H, Schiltz G, Mishra R, et al. Tissue Tranglutaminase Regulates Interactions between Ovarian Cancer Stem Cells and the Tumor Niche. Cancer Res. 2018;78:2990-3001 pubmed publisher
	Han S, Li Z, Master L, Master Z, Wu A. Exogenous IGFBP-2 promotes proliferation, invasion, and chemoresistance to temozolomide in glioma cells via the integrin ?1-ERK pathway. Br J Cancer. 2014;111:1400-9 pubmed publisher
	Rui X, Mehrbod M, Van Agthoven J, Anand S, Xiong J, Mofrad M, et al. The ?-subunit regulates stability of the metal ion at the ligand-associated metal ion-binding site in ?3 integrins. J Biol Chem. 2014;289:23256-63 pubmed publisher
	Loddo M, Andryszkiewicz J, Rodriguez Acebes S, Stoeber K, Jones A, Dafou D, et al. Pregnancy-associated plasma protein A regulates mitosis and is epigenetically silenced in breast cancer. J Pathol. 2014;233:344-56 pubmed publisher
	Lee S, Kim J, Cho S, Kim H, Shin H, Jeon J, et al. TIMP-1 modulates chemotaxis of human neural stem cells through CD63 and integrin signalling. Biochem J. 2014;459:565-76 pubmed publisher
	O Brien X, Loosley A, Oakley K, Tang J, Reichner J. Technical advance: introducing a novel metric, directionality time, to quantify human neutrophil chemotaxis as a function of matrix composition and stiffness. J Leukoc Biol. 2014;95:993-1004 pubmed publisher
	Karki R, Kim S, Kim D. Magnolol inhibits migration of vascular smooth muscle cells via cytoskeletal remodeling pathway to attenuate neointima formation. Exp Cell Res. 2013;319:3238-50 pubmed publisher
	Iwai L, Payne L, Luczynski M, Chang F, Xu H, Clinton R, et al. Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants. Biochem J. 2013;454:501-13 pubmed publisher
	Yang Y, Hong Q, Shi P, Liu Z, Luo J, Shao Z. Elevated expression of syntenin in breast cancer is correlated with lymph node metastasis and poor patient survival. Breast Cancer Res. 2013;15:R50 pubmed publisher
	Blockhuys S, Van Rompaye B, De Rycke R, Lambein K, Claes K, Bracke M, et al. Radiation-induced myosin IIA expression stimulates collagen type I matrix reorganization. Radiother Oncol. 2013;108:162-7 pubmed publisher
	Krishnan M, Lapierre L, Knowles B, Goldenring J. Rab25 regulates integrin expression in polarized colonic epithelial cells. Mol Biol Cell. 2013;24:818-31 pubmed publisher
	Rapraeger A, Ell B, Roy M, Li X, Morrison O, Thomas G, et al. Vascular endothelial-cadherin stimulates syndecan-1-coupled insulin-like growth factor-1 receptor and cross-talk between ?V?3 integrin and vascular endothelial growth factor receptor 2 at the onset of endothelial cell dissemination during angiogenesi. FEBS J. 2013;280:2194-206 pubmed publisher
	Xu H, Bihan D, Chang F, Huang P, Farndale R, Leitinger B. Discoidin domain receptors promote Î±1Î²1- and Î±2Î²1-integrin mediated cell adhesion to collagen by enhancing integrin activation. PLoS ONE. 2012;7:e52209 pubmed publisher
	Vial D, McKeown Longo P. Epidermal growth factor (EGF) regulates ?5?1 integrin activation state in human cancer cell lines through the p90RSK-dependent phosphorylation of filamin A. J Biol Chem. 2012;287:40371-80 pubmed publisher
	Fennewald S, Kantara C, Sastry S, Resto V. Laminin interactions with head and neck cancer cells under low fluid shear conditions lead to integrin activation and binding. J Biol Chem. 2012;287:21058-66 pubmed publisher
	Vincourt J, Etienne S, Grossin L, Cottet J, Bantsimba Malanda C, Netter P, et al. Matrilin-3 switches from anti- to pro-anabolic upon integration to the extracellular matrix. Matrix Biol. 2012;31:290-8 pubmed publisher
	Steinberg F, Heesom K, Bass M, Cullen P. SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways. J Cell Biol. 2012;197:219-30 pubmed publisher
	Malik M, Catherino W. Development and validation of a three-dimensional in vitro model for uterine leiomyoma and patient-matched myometrium. Fertil Steril. 2012;97:1287-93 pubmed publisher
	Nummela P, Lammi J, Soikkeli J, Saksela O, Laakkonen P, Hölttä E. Transforming growth factor beta-induced (TGFBI) is an anti-adhesive protein regulating the invasive growth of melanoma cells. Am J Pathol. 2012;180:1663-74 pubmed publisher
	Kimura H, Tome Y, Momiyama M, Hayashi K, Tsuchiya H, Bouvet M, et al. Imaging the inhibition by anti-?1 integrin antibody of lung seeding of single osteosarcoma cells in live mice. Int J Cancer. 2012;131:2027-33 pubmed publisher
	Haseley A, Boone S, Wojton J, Yu L, Yoo J, Yu J, et al. Extracellular matrix protein CCN1 limits oncolytic efficacy in glioma. Cancer Res. 2012;72:1353-62 pubmed publisher
	Saghizadeh M, Soleymani S, Harounian A, Bhakta B, Troyanovsky S, Brunken W, et al. Alterations of epithelial stem cell marker patterns in human diabetic corneas and effects of c-met gene therapy. Mol Vis. 2011;17:2177-90 pubmed
	Sakai K, Nakamura T, Suzuki Y, Imizu T, Matsumoto K. 3-D collagen-dependent cell surface expression of MT1-MMP and MMP-2 activation regardless of integrin ?1 function and matrix stiffness. Biochem Biophys Res Commun. 2011;412:98-103 pubmed publisher
	Shannon Lowe C, Rowe M. Epstein-Barr virus infection of polarized epithelial cells via the basolateral surface by memory B cell-mediated transfer infection. PLoS Pathog. 2011;7:e1001338 pubmed publisher
	Grzesiak J, Tran Cao H, Burton D, Kaushal S, Vargas F, Clopton P, et al. Knockdown of the ?(1) integrin subunit reduces primary tumor growth and inhibits pancreatic cancer metastasis. Int J Cancer. 2011;129:2905-15 pubmed publisher
	Starke R, Ferraro F, Paschalaki K, Dryden N, McKinnon T, Sutton R, et al. Endothelial von Willebrand factor regulates angiogenesis. Blood. 2011;117:1071-80 pubmed publisher
	Shannon L, Calloway P, Welch T, Vines C. CCR7/CCL21 migration on fibronectin is mediated by phospholipase Cgamma1 and ERK1/2 in primary T lymphocytes. J Biol Chem. 2010;285:38781-7 pubmed publisher
	Xia B, Joubert A, Groves B, Vo K, Ashraf D, Djavaherian D, et al. Modulation of cell adhesion and migration by the histone methyltransferase subunit mDpy-30 and its interacting proteins. PLoS ONE. 2010;5:e11771 pubmed publisher
	Vincourt J, Etienne S, Cottet J, Delaunay C, Malanda C, Malanda B, et al. C-propeptides of procollagens I alpha 1 and II that differentially accumulate in enchondromas versus chondrosarcomas regulate tumor cell survival and migration. Cancer Res. 2010;70:4739-48 pubmed publisher
	Ismail N, Pravda E, Li D, Shih S, Dallabrida S. Angiopoietin-1 reduces H(2)O(2)-induced increases in reactive oxygen species and oxidative damage to skin cells. J Invest Dermatol. 2010;130:1307-17 pubmed publisher
	Drossopoulou G, Tsotakos N, Tsilibary E. Impaired transcription factor interplay in addition to advanced glycation end products suppress podocalyxin expression in high glucose-treated human podocytes. Am J Physiol Renal Physiol. 2009;297:F594-603 pubmed publisher
	Schneider I, Hays C, Waterman C. Epidermal growth factor-induced contraction regulates paxillin phosphorylation to temporally separate traction generation from de-adhesion. Mol Biol Cell. 2009;20:3155-67 pubmed publisher
	Barbolina M, Adley B, Kelly D, Shepard J, Fought A, Scholtens D, et al. Downregulation of connective tissue growth factor by three-dimensional matrix enhances ovarian carcinoma cell invasion. Int J Cancer. 2009;125:816-25 pubmed publisher
	Ksiazek K, Mikula Pietrasik J, Korybalska K, Dworacki G, Jorres A, Witowski J. Senescent peritoneal mesothelial cells promote ovarian cancer cell adhesion: the role of oxidative stress-induced fibronectin. Am J Pathol. 2009;174:1230-40 pubmed publisher
	Rizki A, Mott J, Bissell M. Polo-like kinase 1 is involved in invasion through extracellular matrix. Cancer Res. 2007;67:11106-10 pubmed
	Bhoopathi P, Chetty C, Kunigal S, Vanamala S, Rao J, Lakka S. Blockade of tumor growth due to matrix metalloproteinase-9 inhibition is mediated by sequential activation of beta1-integrin, ERK, and NF-kappaB. J Biol Chem. 2008;283:1545-52 pubmed
	Degen M, Brellier F, Kain R, Ruiz C, Terracciano L, Orend G, et al. Tenascin-W is a novel marker for activated tumor stroma in low-grade human breast cancer and influences cell behavior. Cancer Res. 2007;67:9169-79 pubmed
	Nolz J, Medeiros R, Mitchell J, Zhu P, Freedman B, Shimizu Y, et al. WAVE2 regulates high-affinity integrin binding by recruiting vinculin and talin to the immunological synapse. Mol Cell Biol. 2007;27:5986-6000 pubmed
	Yahiro K, Morinaga N, Satoh M, Matsuura G, Tomonaga T, Nomura F, et al. Identification and characterization of receptors for vacuolating activity of subtilase cytotoxin. Mol Microbiol. 2006;62:480-90 pubmed
	Jung K, Liu X, Chirco R, Fridman R, Kim H. Identification of CD63 as a tissue inhibitor of metalloproteinase-1 interacting cell surface protein. EMBO J. 2006;25:3934-42 pubmed
	Oinuma I, Katoh H, Negishi M. Semaphorin 4D/Plexin-B1-mediated R-Ras GAP activity inhibits cell migration by regulating beta(1) integrin activity. J Cell Biol. 2006;173:601-13 pubmed
	Driss A, Charrier L, Yan Y, Nduati V, Sitaraman S, Merlin D. Dystroglycan receptor is involved in integrin activation in intestinal epithelia. Am J Physiol Gastrointest Liver Physiol. 2006;290:G1228-42 pubmed
	Zaslavsky A, Li S, Xu Y. Sphingosine-1-phosphate induces a PDGFR-dependent cell detachment via inhibiting beta1 integrin in HEK293 cells. FEBS Lett. 2005;579:3899-906 pubmed
	Tucker B, Rahimtula M, Mearow K. Integrin activation and neurotrophin signaling cooperate to enhance neurite outgrowth in sensory neurons. J Comp Neurol. 2005;486:267-80 pubmed
	Janes S, Watt F. Switch from alphavbeta5 to alphavbeta6 integrin expression protects squamous cell carcinomas from anoikis. J Cell Biol. 2004;166:419-31 pubmed

product information

Catalog Number :

MAB1959

Subcategory :

Cell Structure

Product Name :

Anti-Integrin β1 Antibody, clone P5D2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P05556

Host Name :

Mouse

Antigen :

Integrin β1

Clone :

P5D2

Conjugate :

Purified

Isotype :

IgG 2b κ

Product Description :

Anti-Integrin β1 Antibody, clone P5D2

Cross Reactivity :

Human

Background :

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

ALT Names :

CD29;MAB1959Z

Immunogen :

Human keratinocytes derived from skin.

Specificity :

Reacts with Human Integrin Beta 1.

Package Size :

100 μg

Uses :

ELISA;Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Affects Function

Storage :

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.