Integrin β3 Antibody, clone 25E11, azide free | EMD Millipore MAB1957Z product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Integrin β3 Antibody, clone 25E11, azide free

catalog :

MAB1957Z

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

25E11

reactivity :

human

application :

immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

citations: 12

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; fig 3	Artym V, Swatkoski S, Matsumoto K, Campbell C, Petrie R, Dimitriadis E, et al. Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network. J Cell Biol. 2015;208:331-50 pubmed publisher
	Zhang Z, Shively J. Acceleration of bone repair in NOD/SCID mice by human monoosteophils, novel LL-37-activated monocytes. PLoS ONE. 2013;8:e67649 pubmed publisher
	Burnett L, Rahmany M, Richter J, Aboushwareb T, Eberli D, Ward C, et al. Hemostatic properties and the role of cell receptor recognition in human hair keratin protein hydrogels. Biomaterials. 2013;34:2632-40 pubmed publisher
	Cox R, Livesay S, Johnson M, Ohi M, Williams J. The human metapneumovirus fusion protein mediates entry via an interaction with RGD-binding integrins. J Virol. 2012;86:12148-60 pubmed publisher
	Hasebe A, Nakamura Y, Tashima H, Takahashi K, Iijima M, Yoshimoto N, et al. The C-terminal region of NELL1 mediates osteoblastic cell adhesion through integrin ?3?1. FEBS Lett. 2012;586:2500-6 pubmed publisher
	Vincourt J, Etienne S, Grossin L, Cottet J, Bantsimba Malanda C, Netter P, et al. Matrilin-3 switches from anti- to pro-anabolic upon integration to the extracellular matrix. Matrix Biol. 2012;31:290-8 pubmed publisher
	Carrillo L, Arciniegas E, Rojas H, Ram rez R. Immunolocalization of endocan during the endothelial-mesenchymal transition process. Eur J Histochem. 2011;55:e13 pubmed
	Zhang Y, Chen M, Venugopal S, Zhou Y, Xiang W, Li Y, et al. Isthmin exerts pro-survival and death-promoting effect on endothelial cells through alphavbeta5 integrin depending on its physical state. Cell Death Dis. 2011;2:e153 pubmed publisher
	Vincourt J, Etienne S, Cottet J, Delaunay C, Malanda C, Malanda B, et al. C-propeptides of procollagens I alpha 1 and II that differentially accumulate in enchondromas versus chondrosarcomas regulate tumor cell survival and migration. Cancer Res. 2010;70:4739-48 pubmed publisher
	Cseke G, Maginnis M, Cox R, Tollefson S, Podsiad A, Wright D, et al. Integrin alphavbeta1 promotes infection by human metapneumovirus. Proc Natl Acad Sci U S A. 2009;106:1566-71 pubmed publisher
	Short S, Derrien A, Narsimhan R, Lawler J, Ingber D, Zetter B. Inhibition of endothelial cell migration by thrombospondin-1 type-1 repeats is mediated by beta1 integrins. J Cell Biol. 2005;168:643-53 pubmed
	Akimov S, Krylov D, Fleischman L, Belkin A. Tissue transglutaminase is an integrin-binding adhesion coreceptor for fibronectin. J Cell Biol. 2000;148:825-38 pubmed

product information

Catalog Number :

MAB1957Z

Subcategory :

Cell Structure

Product Name :

Anti-Integrin β3 Antibody, clone 25E11, azide free

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P05106

Host Name :

Mouse

Antigen :

Integrin β3

Clone :

25E11

Conjugate :

Purified

Isotype :

IgM

Product Description :

Anti-Integrin β3 Antibody, clone 25E11, azide free

Cross Reactivity :

Human

Background :

The integrin family of cell adhesion receptors consists of several membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Platelet membrane glycoprotein Integrin beta3 or CD61 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets.

ALT Names :

CD61;MAB1957

Immunogen :

Normal blood mononuclear cells activated by mixed lymphocyte reaction and cultured for 10 days in medium containing IL-2.

Specificity :

Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). The protein detectable is a complex of CD41 and CD61. Ligands are fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The monocytoid cell line U937 is highly positive. (original paper claimed IgG2ak)

Package Size :

100 µg

Uses :

Flow Cytometry;Immunoprecipitation;Immunocytochemistry;Affects Function

Storage :

Maintain at 2-8°C.