This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Integrin α5 Antibody, clone P1D6, azide free
catalog :
MAB1956Z
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
P1D6

The same clone is also sold as:
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, proximity ligation assay, blocking or activating experiments
citations: 36
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; human; loading ...; fig 3
  • immunohistochemistry - frozen section; human; loading ...; fig 1
  • flow cytometry; human; loading ...; fig 2
Storm R, Persson B, Skalman L, Frängsmyr L, Lindström M, Rankin G, et al. Human Adenovirus Type 37 Uses αVβ1 and α3β1 Integrins for Infection of Human Corneal Cells. J Virol. 2017;91: pubmed publisher
  • blocking or activating experiments; human; loading ...; fig s1c
Kolanowski S, van Schijndel G, Van Ham S, ten Brinke A. Adaptation to replating of dendritic cells synergizes with Toll-like receptor stimuli and enhances the pro-inflammatory cytokine profile. Cytotherapy. 2016;18:902-10 pubmed publisher
  • immunohistochemistry; human; fig 5a
Walker L, Hussein H, Akula S. Subcellular fractionation method to study endosomal trafficking of Kaposi's sarcoma-associated herpesvirus. Cell Biosci. 2016;6:1 pubmed publisher
  • proximity ligation assay; human; 1:1000; fig s3
Fiore V, Strane P, Bryksin A, White E, Hagood J, Barker T. Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction. J Cell Biol. 2015;211:173-90 pubmed publisher
  • blocking or activating experiments; human
Toh Y, Xing J, Yu H. Modulation of integrin and E-cadherin-mediated adhesions to spatially control heterogeneity in human pluripotent stem cell differentiation. Biomaterials. 2015;50:87-97 pubmed publisher
  • flow cytometry; human; 1:50
Bassagañas S, Carvalho S, Dias A, Pérez Garay M, Ortiz M, Figueras J, et al. Pancreatic cancer cell glycosylation regulates cell adhesion and invasion through the modulation of ?2?1 integrin and E-cadherin function. PLoS ONE. 2014;9:e98595 pubmed publisher
  • blocking or activating experiments; human
  • western blot; human
Wei Y, Zhang Y, Cai H, Mirza A, Iorio R, Peeples M, et al. Roles of the putative integrin-binding motif of the human metapneumovirus fusion (f) protein in cell-cell fusion, viral infectivity, and pathogenesis. J Virol. 2014;88:4338-52 pubmed publisher
  • flow cytometry; human
Majhen D, Stojanović N, Vukić D, Pichon C, Leduc C, Osmak M, et al. Increased adenovirus Type 5 mediated transgene expression due to RhoB down-regulation. PLoS ONE. 2014;9:e86698 pubmed publisher
  • blocking or activating experiments; human; 10 ug/ml
  • flow cytometry; human; 2 ug/25 x104cells
Stampolidis P, Ullrich A, Iacobelli S. LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention. Oncogene. 2015;34:39-52 pubmed publisher
  • blocking or activating experiments; human
Damiano L, Stewart K, Cohet N, Mouw J, Lakins J, Debnath J, et al. Oncogenic targeting of BRM drives malignancy through C/EBP?-dependent induction of ?5 integrin. Oncogene. 2014;33:2441-53 pubmed publisher
  • immunocytochemistry; human
Ho Y, Kok S, Wang J, Lin L. Translucent titanium coating altered the composition of focal adhesions and promoted migration of osteoblast-like MG-63 cells on glass. J Biomed Mater Res A. 2014;102:1187-201 pubmed publisher
Hui T, Sørensen E, Rittling S. Osteopontin binding to the alpha 4 integrin requires highest affinity integrin conformation, but is independent of post-translational modifications of osteopontin. Matrix Biol. 2015;41:19-25 pubmed publisher
Juengel E, Makarevic J, Reiter M, Mani J, Tsaur I, Bartsch G, et al. Resistance to the mTOR inhibitor temsirolimus alters adhesion and migration behavior of renal cell carcinoma cells through an integrin ?5- and integrin ?3-dependent mechanism. Neoplasia. 2014;16:291-300 pubmed publisher
Walker L, Hussein H, Akula S. Disintegrin-like domain of glycoprotein B regulates Kaposi's sarcoma-associated herpesvirus infection of cells. J Gen Virol. 2014;95:1770-82 pubmed publisher
Tanaka R, Seki Y, Saito Y, Kamiya S, Fujita M, Okutsu H, et al. Tenascin-C-derived peptide TNIIIA2 highly enhances cell survival and platelet-derived growth factor (PDGF)-dependent cell proliferation through potentiated and sustained activation of integrin ?5?1. J Biol Chem. 2014;289:17699-708 pubmed publisher
Katagiri F, Takagi M, Nakamura M, Tanaka Y, Hozumi K, Kikkawa Y, et al. Screening of integrin-binding peptides in a laminin peptide library derived from the mouse laminin ? chain short arm regions. Arch Biochem Biophys. 2014;550-551:33-41 pubmed publisher
Kiwanuka E, Andersson L, Caterson E, Junker J, Gerdin B, Eriksson E. CCN2 promotes keratinocyte adhesion and migration via integrin ?5?1. Exp Cell Res. 2013;319:2938-46 pubmed publisher
Vial D, McKeown Longo P. Epidermal growth factor (EGF) regulates ?5?1 integrin activation state in human cancer cell lines through the p90RSK-dependent phosphorylation of filamin A. J Biol Chem. 2012;287:40371-80 pubmed publisher
Teo G, Ankrum J, Martinelli R, Boetto S, Simms K, Sciuto T, et al. Mesenchymal stem cells transmigrate between and directly through tumor necrosis factor-?-activated endothelial cells via both leukocyte-like and novel mechanisms. Stem Cells. 2012;30:2472-86 pubmed publisher
Tsaur I, Makarevic J, Juengel E, Gasser M, Waaga Gasser A, Kurosch M, et al. Resistance to the mTOR-inhibitor RAD001 elevates integrin ?2- and ?1-triggered motility, migration and invasion of prostate cancer cells. Br J Cancer. 2012;107:847-55 pubmed publisher
Matsunaga T, Fukai F, Kameda T, Shide K, Shimoda H, Torii E, et al. Potentiated activation of VLA-4 and VLA-5 accelerates proplatelet-like formation. Ann Hematol. 2012;91:1633-43 pubmed publisher
Ma J, Cui W, He S, Duan Y, Heng L, Wang L, et al. Human U87 astrocytoma cell invasion induced by interaction of ?ig-h3 with integrin ?5?1 involves calpain-2. PLoS ONE. 2012;7:e37297 pubmed publisher
Li Q, Ding X, Thomas J, Harding C, Pecora N, Ziady A, et al. Rv2468c, a novel Mycobacterium tuberculosis protein that costimulates human CD4+ T cells through VLA-5. J Leukoc Biol. 2012;91:311-20 pubmed publisher
Sabari J, Lax D, Connors D, Brotman I, Mindrebo E, Butler C, et al. Fibronectin matrix assembly suppresses dispersal of glioblastoma cells. PLoS ONE. 2011;6:e24810 pubmed publisher
Nägele V, Heesemann J, Schielke S, Jimenez Soto L, Kurzai O, Ackermann N. Neisseria meningitidis adhesin NadA targets beta1 integrins: functional similarity to Yersinia invasin. J Biol Chem. 2011;286:20536-46 pubmed publisher
Teo J, Ng R, Ng C, Lin A. Surface characteristics of acrylic modified polysulfone membranes improves renal proximal tubule cell adhesion and spreading. Acta Biomater. 2011;7:2060-9 pubmed publisher
Morgan M, Jazayeri M, Ramsay A, Thomas G, Boulanger M, Hart I, et al. Psoriasin (S100A7) associates with integrin ?6 subunit and is required for ?v?6-dependent carcinoma cell invasion. Oncogene. 2011;30:1422-35 pubmed publisher
Lim S, Kreipe B, Trzeciakowski J, DANGOTT L, Trache A. Extracellular matrix effect on RhoA signaling modulation in vascular smooth muscle cells. Exp Cell Res. 2010;316:2833-48 pubmed publisher
Han S, Arnold S, Sithu S, Mahoney E, Geralds J, Tran P, et al. Rescuing vasculature with intravenous angiopoietin-1 and alpha v beta 3 integrin peptide is protective after spinal cord injury. Brain. 2010;133:1026-42 pubmed publisher
Kessentini Zouari R, Jebali J, Taboubi S, Srairi Abid N, Morjen M, Kallech Ziri O, et al. CC-PLA2-1 and CC-PLA2-2, two Cerastes cerastes venom-derived phospholipases A2, inhibit angiogenesis both in vitro and in vivo. Lab Invest. 2010;90:510-9 pubmed publisher
Friedrichs J, Helenius J, Muller D. Stimulated single-cell force spectroscopy to quantify cell adhesion receptor crosstalk. Proteomics. 2010;10:1455-62 pubmed publisher
Stratman A, Saunders W, Sacharidou A, Koh W, Fisher K, Zawieja D, et al. Endothelial cell lumen and vascular guidance tunnel formation requires MT1-MMP-dependent proteolysis in 3-dimensional collagen matrices. Blood. 2009;114:237-47 pubmed publisher
Westhorpe C, Zhou J, Webster N, Kalionis B, Lewin S, Jaworowski A, et al. Effects of HIV-1 infection in vitro on transendothelial migration by monocytes and monocyte-derived macrophages. J Leukoc Biol. 2009;85:1027-35 pubmed publisher
Fukushima Y, Tamura M, Nakagawa H, Itoh K. Induction of glioma cell migration by vitronectin in human serum and cerebrospinal fluid. J Neurosurg. 2007;107:578-85 pubmed
Schwarz M, Zheng H, Liu J, Corbett S, Schwarz R. Endothelial-monocyte activating polypeptide II alters fibronectin based endothelial cell adhesion and matrix assembly via alpha5 beta1 integrin. Exp Cell Res. 2005;311:229-39 pubmed
Mazzocca A, Coppari R, De Franco R, Cho J, Libermann T, Pinzani M, et al. A secreted form of ADAM9 promotes carcinoma invasion through tumor-stromal interactions. Cancer Res. 2005;65:4728-38 pubmed
product information
Catalog Number :
MAB1956Z
Subcategory :
Cell Structure
Product Name :
Anti-Integrin α5 Antibody, clone P1D6, azide free
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P08648
Host Name :
Mouse
Antigen :
Integrin α5
Clone :
P1D6
Conjugate :
Purified
Isotype :
IgG3
Product Description :
Anti-Integrin α5 Antibody, clone P1D6, azide free
Cross Reactivity :
Human
Background :
Integrin alpha 5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell surface mediated signalling.
ALT Names :
CD49e;MAB1956
Specificity :
Reacts with Human alpha 5 integrin. Reactivity with other species is unknown.
Package Size :
100 μg
Uses :
Immunohistochemistry;Affects Function
Storage :
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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