Integrin α3 Antibody, clone P1B5, azide free | EMD Millipore MAB1952Z product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Integrin α3 Antibody, clone P1B5, azide free

catalog :

MAB1952Z

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P1B5

The same clone is also sold as:

Developmental Studies Hybridoma Bank: P1B5
BioLegend: 921601, 865201, 865204, 865203, 865202
Santa Cruz Biotechnology: sc-13545
Invitrogen: 17-0494-42
LifeSpan Biosciences: LS-C187531, LS-B13291
Bio-Rad: MCA5694GA, MCA5694T, MCA5694, MCA5694F

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments

citations: 46

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; loading ...; fig 3 flow cytometry; human; loading ...; fig 2 immunocytochemistry; human; loading ...; fig 6a	Storm R, Persson B, Skalman L, Frängsmyr L, Lindström M, Rankin G, et al. Human Adenovirus Type 37 Uses αVβ1 and α3β1 Integrins for Infection of Human Corneal Cells. J Virol. 2017;91: pubmed publisher
immunohistochemistry - paraffin section; human	Yalcin E, He Y, Orhan D, Pazzagli C, Emiralioğlu N, Has C. Crucial role of posttranslational modifications of integrin Î±3 in interstitial lung disease and nephrotic syndrome. Hum Mol Genet. 2015;24:3679-88 pubmed publisher
blocking or activating experiments; human; fig 3	Artym V, Swatkoski S, Matsumoto K, Campbell C, Petrie R, Dimitriadis E, et al. Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network. J Cell Biol. 2015;208:331-50 pubmed publisher
flow cytometry; human; 1:50	Bassagañas S, Carvalho S, Dias A, Pérez Garay M, Ortiz M, Figueras J, et al. Pancreatic cancer cell glycosylation regulates cell adhesion and invasion through the modulation of ?2?1 integrin and E-cadherin function. PLoS ONE. 2014;9:e98595 pubmed publisher
western blot; human	Glinskii O, Li F, Wilson L, Barnes S, Rittenhouse Olson K, Barchi J, et al. Endothelial integrin ?3?1 stabilizes carbohydrate-mediated tumor/endothelial cell adhesion and induces macromolecular signaling complex formation at the endothelial cell membrane. Oncotarget. 2014;5:1382-9 pubmed
blocking or activating experiments; human; 10 ug/ml flow cytometry; human; 2 ug/25 x104cells	Stampolidis P, Ullrich A, Iacobelli S. LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention. Oncogene. 2015;34:39-52 pubmed publisher
blocking or activating experiments; human flow cytometry; human immunocytochemistry; human	Kikkawa Y, Ogawa T, Sudo R, Yamada Y, Katagiri F, Hozumi K, et al. The lutheran/basal cell adhesion molecule promotes tumor cell migration by modulating integrin-mediated cell attachment to laminin-511 protein. J Biol Chem. 2013;288:30990-1001 pubmed publisher
blocking or activating experiments; human	Racicot K, Cardenas I, WÃ¼nsche V, Aldo P, Guller S, Means R, et al. Viral infection of the pregnant cervix predisposes to ascending bacterial infection. J Immunol. 2013;191:934-41 pubmed publisher
immunocytochemistry; human	Ho Y, Kok S, Wang J, Lin L. Translucent titanium coating altered the composition of focal adhesions and promoted migration of osteoblast-like MG-63 cells on glass. J Biomed Mater Res A. 2014;102:1187-201 pubmed publisher
flow cytometry; human	Kligys K, Wu Y, Hamill K, Lewandowski K, Hopkinson S, Budinger G, et al. Laminin-332 and ?3?1 integrin-supported migration of bronchial epithelial cells is modulated by fibronectin. Am J Respir Cell Mol Biol. 2013;49:731-40 pubmed publisher
blocking or activating experiments; human	Wondimu Z, Omrani S, Ishikawa T, Javed F, Oikawa Y, Virtanen I, et al. A novel monoclonal antibody to human laminin ?5 chain strongly inhibits integrin-mediated cell adhesion and migration on laminins 511 and 521. PLoS ONE. 2013;8:e53648 pubmed publisher
	Wang H, Jin H, Beauvais D, Rapraeger A. Cytoplasmic domain interactions of syndecan-1 and syndecan-4 with Î±6Î²4 integrin mediate human epidermal growth factor receptor (HER1 and HER2)-dependent motility and survival. J Biol Chem. 2014;289:30318-32 pubmed publisher
	Katagiri F, Hara T, Yamada Y, Urushibata S, Hozumi K, Kikkawa Y, et al. Biological activities of the homologous loop regions in the laminin ? chain LG modules. Biochemistry. 2014;53:3699-708 pubmed publisher
	Katagiri F, Takagi M, Nakamura M, Tanaka Y, Hozumi K, Kikkawa Y, et al. Screening of integrin-binding peptides in a laminin peptide library derived from the mouse laminin ? chain short arm regions. Arch Biochem Biophys. 2014;550-551:33-41 pubmed publisher
	Park S, Yun J, Kim J, Kim K, Cho C, Kim J. Angiopoietin 2 induces pericyte apoptosis via ?3?1 integrin signaling in diabetic retinopathy. Diabetes. 2014;63:3057-68 pubmed publisher
	Zwolanek D, Veit G, Eble J, Gullberg D, Ruggiero F, Heino J, et al. Collagen XXII binds to collagen-binding integrins via the novel motifs GLQGER and GFKGER. Biochem J. 2014;459:217-27 pubmed publisher
	Pochec E, Janik M, Hoja Łukowicz D, Link Lenczowski P, Przybyło M, Litynska A. Expression of integrins ?3?1 and ?5?1 and GlcNAc ?1,6 glycan branching influences metastatic melanoma cell migration on fibronectin. Eur J Cell Biol. 2013;92:355-62 pubmed publisher
	Palmer T, Martinez C, Vasquez C, Hebron K, Jones Paris C, Arnold S, et al. Integrin-free tetraspanin CD151 can inhibit tumor cell motility upon clustering and is a clinical indicator of prostate cancer progression. Cancer Res. 2014;74:173-87 pubmed publisher
	Serres E, Debarbieux F, Stanchi F, Maggiorella L, Grall D, Turchi L, et al. Fibronectin expression in glioblastomas promotes cell cohesion, collective invasion of basement membrane in vitro and orthotopic tumor growth in mice. Oncogene. 2014;33:3451-62 pubmed publisher
	Otagiri D, Yamada Y, Hozumi K, Katagiri F, Kikkawa Y, Nomizu M. Cell attachment and spreading activity of mixed laminin peptide-chitosan membranes. Biopolymers. 2013;100:751-9 pubmed publisher
	Saito D, Kyakumoto S, Chosa N, Ibi M, Takahashi N, Okubo N, et al. Transforming growth factor-?1 induces epithelial-mesenchymal transition and integrin ?3?1-mediated cell migration of HSC-4 human squamous cell carcinoma cells through Slug. J Biochem. 2013;153:303-15 pubmed publisher
	Hamill K, Hopkinson S, Skalli O, Jones J. Actinin-4 in keratinocytes regulates motility via an effect on lamellipodia stability and matrix adhesions. FASEB J. 2013;27:546-56 pubmed publisher
	Hozumi K, Ishikawa M, Hayashi T, Yamada Y, Katagiri F, Kikkawa Y, et al. Identification of cell adhesive sequences in the N-terminal region of the laminin ?2 chain. J Biol Chem. 2012;287:25111-22 pubmed publisher
	Kligys K, Wu Y, Hopkinson S, Kaur S, Platanias L, Jones J. ?6?4 integrin, a master regulator of expression of integrins in human keratinocytes. J Biol Chem. 2012;287:17975-84 pubmed publisher
	Katagiri F, Ishikawa M, Yamada Y, Hozumi K, Kikkawa Y, Nomizu M. Screening of integrin-binding peptides from the laminin ?4 and ?5 chain G domain peptide library. Arch Biochem Biophys. 2012;521:32-42 pubmed publisher
	Zhang H, Guo F, Zhu G. Involvement of host cell integrin ?2 in Cryptosporidium parvum infection. Infect Immun. 2012;80:1753-8 pubmed publisher
	Lee C, Marzan D, Lin G, Goodison S, Silletti S. ?2 Integrin-Dependent Suppression of Pancreatic Adenocarcinoma Cell Invasion Involves Ectodomain Regulation of Kallikrein-Related Peptidase-5. J Oncol. 2011;2011:365651 pubmed publisher
	Kikkawa Y, Miwa T, Tohara Y, Hamakubo T, Nomizu M. An antibody to the lutheran glycoprotein (Lu) recognizing the LU4 blood type variant inhibits cell adhesion to laminin ?5. PLoS ONE. 2011;6:e23329 pubmed publisher
	Caiado F, Carvalho T, Silva F, Castro C, Clode N, Dye J, et al. The role of fibrin E on the modulation of endothelial progenitors adhesion, differentiation and angiogenic growth factor production and the promotion of wound healing. Biomaterials. 2011;32:7096-105 pubmed publisher
	Yamada Y, Katagiri F, Hozumi K, Kikkawa Y, Nomizu M. Cell behavior on protein matrices containing laminin ?1 peptide AG73. Biomaterials. 2011;32:4327-35 pubmed publisher
	Kusuma N, Denoyer D, Eble J, Redvers R, Parker B, Pelzer R, et al. Integrin-dependent response to laminin-511 regulates breast tumor cell invasion and metastasis. Int J Cancer. 2012;130:555-66 pubmed publisher
	VanderWyst S, Perkumas K, Read A, Overby D, Stamer W. Structural basement membrane components and corresponding integrins in Schlemm's canal endothelia. Mol Vis. 2011;17:199-209 pubmed
	Teo J, Ng R, Ng C, Lin A. Surface characteristics of acrylic modified polysulfone membranes improves renal proximal tubule cell adhesion and spreading. Acta Biomater. 2011;7:2060-9 pubmed publisher
	Hozumi K, Akizuki T, Yamada Y, Hara T, Urushibata S, Katagiri F, et al. Cell adhesive peptide screening of the mouse laminin ?1 chain G domain. Arch Biochem Biophys. 2010;503:213-22 pubmed publisher
	Ghosh S, Koblinski J, Johnson J, Liu Y, Ericsson A, Davis J, et al. Urinary-type plasminogen activator receptor/alpha 3 beta 1 integrin signaling, altered gene expression, and oral tumor progression. Mol Cancer Res. 2010;8:145-58 pubmed publisher
	Kessentini Zouari R, Jebali J, Taboubi S, Srairi Abid N, Morjen M, Kallech Ziri O, et al. CC-PLA2-1 and CC-PLA2-2, two Cerastes cerastes venom-derived phospholipases A2, inhibit angiogenesis both in vitro and in vivo. Lab Invest. 2010;90:510-9 pubmed publisher
	Ozawa T, Tsuruta D, Jones J, Ishii M, Ikeda K, Harada T, et al. Dynamic relationship of focal contacts and hemidesmosome protein complexes in live cells. J Invest Dermatol. 2010;130:1624-35 pubmed publisher
	Phan H, Sugino M, Niimi T. The production of recombinant human laminin-332 in a Leishmania tarentolae expression system. Protein Expr Purif. 2009;68:79-84 pubmed publisher
	Stratman A, Saunders W, Sacharidou A, Koh W, Fisher K, Zawieja D, et al. Endothelial cell lumen and vascular guidance tunnel formation requires MT1-MMP-dependent proteolysis in 3-dimensional collagen matrices. Blood. 2009;114:237-47 pubmed publisher
	Ohara T, Kawashiri S, Tanaka A, Noguchi N, Kitahara H, Okamune A, et al. Integrin expression levels correlate with invasion, metastasis and prognosis of oral squamous cell carcinoma. Pathol Oncol Res. 2009;15:429-36 pubmed publisher
	Fiorilli P, Partridge D, Staniszewska I, Wang J, Grabacka M, So K, et al. Integrins mediate adhesion of medulloblastoma cells to tenascin and activate pathways associated with survival and proliferation. Lab Invest. 2008;88:1143-56 pubmed publisher
	Sawai H, Okada Y, Funahashi H, Takahashi H, Matsuo Y, Yasuda A, et al. Basement membrane proteins play an important role in the invasive processes of human pancreatic cancer cells. J Surg Res. 2008;144:117-23 pubmed
	Sroka T, Pennington M, Cress A. Synthetic D-amino acid peptide inhibits tumor cell motility on laminin-5. Carcinogenesis. 2006;27:1748-57 pubmed
	Yuen H, Ziober A, Gopal P, Nasrallah I, Falls E, Meneguzzi G, et al. Suppression of laminin-5 expression leads to increased motility, tumorigenicity, and invasion. Exp Cell Res. 2005;309:198-210 pubmed
	Mazzocca A, Coppari R, De Franco R, Cho J, Libermann T, Pinzani M, et al. A secreted form of ADAM9 promotes carcinoma invasion through tumor-stromal interactions. Cancer Res. 2005;65:4728-38 pubmed
	Benini S, Perbal B, Zambelli D, Colombo M, Manara M, Serra M, et al. In Ewing's sarcoma CCN3(NOV) inhibits proliferation while promoting migration and invasion of the same cell type. Oncogene. 2005;24:4349-61 pubmed

product information

Catalog Number :

MAB1952Z

Subcategory :

Cell Structure

Product Name :

Anti-Integrin α3 Antibody, clone P1B5, azide free

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P26006

Host Name :

Mouse

Antigen :

Integrin α3

Clone :

P1B5

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Integrin α3 Antibody, clone P1B5, azide free

Cross Reactivity :

Human

Background :

Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.

ALT Names :

CD49c;MAB1952

Specificity :

Reacts with Human alpha3 integrin.

Package Size :

100 μg

Uses :

Immunocytochemistry;Immunohistochemistry (Paraffin);Affects Function

Storage :

Maintain at 2–8°C in undiluted aliquots for up to 6 months after date of receipt. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.