Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone A7L6 | EMD Millipore MAB1948P product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone A7L6

catalog :

MAB1948P

quantity :

100 μg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

A7L6

The same clone is also sold as:

Santa Cruz Biotechnology: sc-33707
Invitrogen: MA1-06821, MA5-14641
LifeSpan Biosciences: LS-C88866, LS-C85362, LS-C88431, LS-B10057, LS-C86884, LS-C88432
OriGene: DM3099P
Abnova: MAB13313, MAB12489, MAB13314
Novus Biologicals: NBP2-44448-0.1mg, NBP2-47695-0.1mg
Abcam: ab2501

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig 7e	Kim J, Kim Y, Kim J, Park D, Bae H, Lee D, et al. YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation. J Clin Invest. 2017;127:3441-3461 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 7	Foltz S, Luan J, Call J, Patel A, Peissig K, Fortunato M, et al. Four-week rapamycin treatment improves muscular dystrophy in a fukutin-deficient mouse model of dystroglycanopathy. Skelet Muscle. 2016;6:20 pubmed publisher
western blot; human; 1:500; fig 3	Reine T, Vuong T, Rutkovskiy A, Meen A, Vaage J, Jenssen T, et al. Serglycin in Quiescent and Proliferating Primary Endothelial Cells. PLoS ONE. 2015;10:e0145584 pubmed publisher
western blot; rat; loading ...; fig 9d	Colombelli C, Palmisano M, Eshed Eisenbach Y, Zambroni D, Pavoni E, Ferri C, et al. Perlecan is recruited by dystroglycan to nodes of Ranvier and binds the clustering molecule gliomedin. J Cell Biol. 2015;208:313-29 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:50	Reine T, Grøndahl F, Jenssen T, Hadler Olsen E, Prydz K, Kolset S. Reduced sulfation of chondroitin sulfate but not heparan sulfate in kidneys of diabetic db/db mice. J Histochem Cytochem. 2013;61:606-16 pubmed publisher
	Kyprianou C, Christodoulou N, Hamilton R, Nahaboo W, Boomgaard D, Amadei G, et al. Basement membrane remodelling regulates mouse embryogenesis. Nature. 2020;582:253-258 pubmed publisher
	Hirata A, Katayama K, Tsuji T, Natsume N, Sugahara T, Koga Y, et al. Heparanase localization during palatogenesis in mice. Biomed Res Int. 2013;2013:760236 pubmed publisher
	Bangratz M, Sarrazin N, Devaux J, Zambroni D, Echaniz Laguna A, Rene F, et al. A mouse model of Schwartz-Jampel syndrome reveals myelinating Schwann cell dysfunction with persistent axonal depolarization in vitro and distal peripheral nerve hyperexcitability when perlecan is lacking. Am J Pathol. 2012;180:2040-55 pubmed publisher
	Meech R, Gonzalez K, Barro M, Gromova A, Zhuang L, Hulin J, et al. Barx2 is expressed in satellite cells and is required for normal muscle growth and regeneration. Stem Cells. 2012;30:253-65 pubmed publisher
	Doppler K, Lindner A, Schütz W, Schütz M, Bornemann A. Gain and loss of extracellular molecules in sporadic inclusion body myositis and polymyositis--a proteomics-based study. Brain Pathol. 2012;22:32-40 pubmed publisher
	Ellis A, Pan W, Yang G, Jones K, Chuang C, Whitelock J, et al. Similarity of recombinant human perlecan domain 1 by alternative expression systems bioactive heterogenous recombinant human perlecan D1. BMC Biotechnol. 2010;10:66 pubmed publisher
	Chen S, Wassenhove McCarthy D, Yamaguchi Y, Holzman L, van Kuppevelt T, Orr A, et al. Podocytes require the engagement of cell surface heparan sulfate proteoglycans for adhesion to extracellular matrices. Kidney Int. 2010;78:1088-99 pubmed publisher
	Yang W, Su T, Yang Y, Chang S, Chen C, Chen C. Altered perlecan expression in placental development and gestational diabetes mellitus. Placenta. 2005;26:780-8 pubmed

product information

Catalog Number :

MAB1948P

Subcategory :

Cell Structure

Product Name :

Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone A7L6

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P98160

Host Name :

Rat

Antigen :

Heparan Sulfate Proteoglycan (Perlecan)

Clone :

A7L6

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone A7L6

Cross Reactivity :

Human

Background :

Heparan sulfate proteoglycans (HSPGs) are found on cell surfaces and in the extracelluar matrix of all mammalian tissues, particularly in the core of the basement membrane. HSPGs found in the brain are dystroglycan, N-syndecan, glypican, and perlecan. The composition of HSPGs differs in the protein core, and is thought to determine the location of HSPG in the cell membrane (syndecan and glypican) and extracellular matrix (perlecan and dystroglycan). The postulated functions of HSPG include cell proliferation, differentiation, adhesion, migration, and morphogenesis. Heparan Sulfate Proteoglycan antibodies, in tissues, react strongly and uniformly with basement membranes. Clone A7L6 recognizes domain IV of the core protein of the large heparan sulphate proteoglycan or perlecan. The reactivity is independent of the galactosaminoglycan moieties; therefore, the epitope is not sensitive to heparinase treatment.

ALT Names :

heparan sulfate proteoglycan 2;Schwartz-Jampel syndrome 1 (chondrodystrophic myotonia);endorepellin (domain V region);perlecan proteoglycan;heparan sulfate proteoglycan of basement membrane

Immunogen :

Heparan Sulfate Proteoglycan from EHS mouse tumor

Specificity :

The antibody recognizes a high molecular weight core protein of Heparan Sulfate Proteoglycan (Perlecan). No cross reactivity to laminin, collagen IV, entactin/nidogen, or fibronectin (Horiguchi, 1989).

Package Size :

100 μg

Uses :

Immunohistochemistry;Immunocytochemistry;Western Blotting;Immunoprecipitation

Storage :

Stable for 1 year at 2-8°C from date of receipt