Integrin β1D Antibody, CT cytoplasmic domain, clone 2B1 | EMD Millipore MAB1900 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Integrin β1D Antibody, CT cytoplasmic domain, clone 2B1

catalog :

MAB1900

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2B1

The same clone is also sold as:

Abcam: ab8991
Invitrogen: MA1-06906
Novus Biologicals: NBP1-97734
OriGene: BM6028P
Santa Cruz Biotechnology: sc-59973
LifeSpan Biosciences: LS-C24780

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

citations: 20

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:250; fig 6 western blot; mouse; 1:500; fig 6 western blot; rat; 1:500; fig 6s1	Vanhoutte D, Schips T, Kwong J, Davis J, Tjondrokoesoemo A, Brody M, et al. Thrombospondin expression in myofibers stabilizes muscle membranes. elife. 2016;5: pubmed publisher
western blot; rat; fig 5e	Lin Y, Warren C, Li J, McKinsey T, Russell B. Myofibril growth during cardiac hypertrophy is regulated through dual phosphorylation and acetylation of the actin capping protein CapZ. Cell Signal. 2016;28:1015-24 pubmed publisher
western blot; mouse; 1:200; fig 5, 6	Tjondrokoesoemo A, Schips T, Kanisicak O, Sargent M, Molkentin J. Genetic overexpression of Serpina3n attenuates muscular dystrophy in mice. Hum Mol Genet. 2016;25:1192-202 pubmed publisher
western blot; mouse; 1:200; fig 5	Parvatiyar M, Marshall J, Nguyen R, Jordan M, Richardson V, Roos K, et al. Sarcospan Regulates Cardiac Isoproterenol Response and Prevents Duchenne Muscular Dystrophy-Associated Cardiomyopathy. J Am Heart Assoc. 2015;4: pubmed publisher
immunocytochemistry; mouse; 1:40; loading ...; fig 7q	Van Ry P, Wuebbles R, Key M, Burkin D. Galectin-1 Protein Therapy Prevents Pathology and Improves Muscle Function in the mdx Mouse Model of Duchenne Muscular Dystrophy. Mol Ther. 2015;23:1285-1297 pubmed publisher
western blot; mouse; 1:200; fig 4	Marshall J, Oh J, Chou E, Lee J, Holmberg J, Burkin D, et al. Sarcospan integration into laminin-binding adhesion complexes that ameliorate muscular dystrophy requires utrophin and α7 integrin. Hum Mol Genet. 2015;24:2011-22 pubmed publisher
western blot; rat	Ogasawara R, Nakazato K, Sato K, Boppart M, Fujita S. Resistance exercise increases active MMP and Î²1-integrin protein expression in skeletal muscle. Physiol Rep. 2014;2: pubmed publisher
immunocytochemistry; mouse western blot; mouse	Peter A, Ko C, Kim M, Hsu N, Ouchi N, Rhie S, et al. Myogenic Akt signaling upregulates the utrophin-glycoprotein complex and promotes sarcolemma stability in muscular dystrophy. Hum Mol Genet. 2009;18:318-27 pubmed publisher
	Sengupta S, Rothenberg K, Li H, Hoffman B, Bursac N. Altering integrin engagement regulates membrane localization of Kir2.1 channels. J Cell Sci. 2019;132: pubmed publisher
	Schips T, Vanhoutte D, Vo A, Correll R, Brody M, Khalil H, et al. Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability. Nat Commun. 2019;10:76 pubmed publisher
	Li J, Tanhehco E, Russell B. Actin dynamics is rapidly regulated by the PTEN and PIP2 signaling pathways leading to myocyte hypertrophy. Am J Physiol Heart Circ Physiol. 2014;307:H1618-25 pubmed publisher
	Marshall J, Holmberg J, Chou E, Ocampo A, Oh J, Lee J, et al. Sarcospan-dependent Akt activation is required for utrophin expression and muscle regeneration. J Cell Biol. 2012;197:1009-27 pubmed publisher
	Cabrera P, Pang M, Marshall J, Kung R, Nelson S, Stalnaker S, et al. High throughput screening for compounds that alter muscle cell glycosylation identifies new role for N-glycans in regulating sarcolemmal protein abundance and laminin binding. J Biol Chem. 2012;287:22759-70 pubmed publisher
	Straadt I, Rasmussen M, Young J, Bertram H. Any link between integrin degradation and water-holding capacity in pork?. Meat Sci. 2008;80:722-7 pubmed publisher
	Zhang W, Lonergan S, Gardner M, Huff Lonergan E. Contribution of postmortem changes of integrin, desmin and ?-calpain to variation in water holding capacity of pork. Meat Sci. 2006;74:578-85 pubmed publisher
	Fujita M, Mitsuhashi H, Isogai S, Nakata T, Kawakami A, Nonaka I, et al. Filamin C plays an essential role in the maintenance of the structural integrity of cardiac and skeletal muscles, revealed by the medaka mutant zacro. Dev Biol. 2012;361:79-89 pubmed publisher
	Bauer R, Ratzinger S, Wales L, Bosserhoff A, Senner V, Grifka J, et al. Inhibition of collagen XVI expression reduces glioma cell invasiveness. Cell Physiol Biochem. 2011;27:217-26 pubmed publisher
	Hayashi Y, Mizuno K, Kojima Y, Moritoki Y, Nishio H, Kato T, et al. Characterization of the urethral plate and the underlying tissue defined by expression of collagen subtypes and microarchitecture in hypospadias. Int J Urol. 2011;18:317-22 pubmed publisher
	Ratzinger S, Grassel S, Dowejko A, Reichert T, Bauer R. Induction of type XVI collagen expression facilitates proliferation of oral cancer cells. Matrix Biol. 2011;30:118-25 pubmed publisher
	Kramerova I, Kudryashova E, Wu B, Spencer M. Regulation of the M-cadherin-beta-catenin complex by calpain 3 during terminal stages of myogenic differentiation. Mol Cell Biol. 2006;26:8437-47 pubmed

product information

Catalog Number :

MAB1900

Subcategory :

Cell Structure

Product Name :

Anti-Integrin β1D Antibody, CT cytoplasmic domain, clone 2B1

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P05556

Host Name :

Mouse

Antigen :

Integrin β1D

Clone :

2B1

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Integrin β1D Antibody, CT cytoplasmic domain, clone 2B1

Cross Reactivity :

Human;Mouse;Canine

Background :

Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated alpha and beta subunits. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. There are two major forms of integrin beta 1:beta 1A and beta 1D which differ in 13 amino acids. Their distribution pattern in adult tissues is mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle, which instead express the beta1D variant.

ALT Names :

CD29

Immunogen :

Synthetic peptide including an appending N-terminal cysteine: CQENPIYKSPINNFKNPNYGRKAGL coupled to KLH.

Specificity :

MAB1900 is specific for the cytoplasmic domain of integrin beta1D subunit. Integrin beta1D is present in cardiac and skeletal muscle. In proliferating C2C12 cells, beta1D is not expressed; C2C12 after differentiation into myotubes, however, express high levels of beta1D integrin. beta1D integrin was also found expressed in adult mouse extracts from heat, hind limb muscle, and tongue, with a faint band in skin. No reactivity was observed in extracts from lung, kidney, liver, or spleen {van der Flier et al, 1997}.

Package Size :

100 µg

Uses :

Western Blotting;Immunocytochemistry;Immunohistochemistry

Storage :

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.