Tubulin Antibody, clone YL1/2 | EMD Millipore MAB1864 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Tubulin Antibody, clone YL1/2

catalog :

MAB1864

quantity :

100 µg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

YL1/2

reactivity :

human, mouse, fruit fly

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, radioimmunoassay

citations: 64

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly ; 1:500; loading ...; fig 3b	Smith S, Davidson L, Rebeiz M. Evolutionary expansion of apical extracellular matrix is required for the elongation of cells in a novel structure. elife. 2020;9: pubmed publisher
immunocytochemistry; human; 1:1000; loading ...; fig 1a	Dong C, Xu H, Zhang R, Tanaka N, Takeichi M, Meng W. CAMSAP3 accumulates in the pericentrosomal area and accompanies microtubule release from the centrosome via katanin. J Cell Sci. 2017;130:1709-1715 pubmed publisher
immunocytochemistry; fruit fly ; 1:500; loading ...; fig 6a	Qu Y, Hahn I, Webb S, Pearce S, Prokop A. Periodic actin structures in neuronal axons are required to maintain microtubules. Mol Biol Cell. 2017;28:296-308 pubmed publisher
immunocytochemistry; human; 1:1000; fig 4 western blot; human; 1:5000; fig s2	Bertolin G, Sizaire F, Herbomel G, Reboutier D, Prigent C, Tramier M. A FRET biosensor reveals spatiotemporal activation and functions of aurora kinase A in living cells. Nat Commun. 2016;7:12674 pubmed publisher
immunocytochemistry; human; fig 3	Ruane P, Gumy L, Bola B, Anderson B, Wozniak M, Hoogenraad C, et al. Tumour Suppressor Adenomatous Polyposis Coli (APC) localisation is regulated by both Kinesin-1 and Kinesin-2. Sci Rep. 2016;6:27456 pubmed publisher
immunocytochemistry; mouse; 1:500; fig s5a	Itoh Y, Higuchi M, Oishi K, Kishi Y, Okazaki T, Sakai H, et al. PDK1-Akt pathway regulates radial neuronal migration and microtubules in the developing mouse neocortex. Proc Natl Acad Sci U S A. 2016;113:E2955-64 pubmed publisher
western blot; mouse; 0.5 ug/ml; fig 5	Stritt S, Nurden P, Favier R, Favier M, Ferioli S, Gotru S, et al. Defects in TRPM7 channel function deregulate thrombopoiesis through altered cellular Mg(2+) homeostasis and cytoskeletal architecture. Nat Commun. 2016;7:11097 pubmed publisher
immunocytochemistry; human; fig 3	Lasek A, McPherson B, Trueman N, Burkard M. The Functional Significance of Posttranslational Modifications on Polo-Like Kinase 1 Revealed by Chemical Genetic Complementation. PLoS ONE. 2016;11:e0150225 pubmed publisher
immunocytochemistry; mouse	Okamoto M, Iguchi T, Hattori T, Matsuzaki S, Koyama Y, Taniguchi M, et al. DBZ regulates cortical cell positioning and neurite development by sustaining the anterograde transport of Lis1 and DISC1 through control of Ndel1 dual-phosphorylation. J Neurosci. 2015;35:2942-58 pubmed publisher
immunocytochemistry; fruit fly ; 1:100; fig s6	Williams L, Ganguly S, Loiseau P, Ng B, Palacios I. The auto-inhibitory domain and ATP-independent microtubule-binding region of Kinesin heavy chain are major functional domains for transport in the Drosophila germline. Development. 2014;141:176-86 pubmed publisher
western blot; human; fig 1	Cheng X, Chapple S, Patel B, Puszyk W, Sugden D, Yin X, et al. Gestational diabetes mellitus impairs Nrf2-mediated adaptive antioxidant defenses and redox signaling in fetal endothelial cells in utero. Diabetes. 2013;62:4088-97 pubmed publisher
	Hou Y, Okuda K, Edwards C, Martinez D, Asakura T, Dinnon K, et al. SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract. Cell. 2020;182:429-446.e14 pubmed publisher
	Pascolutti R, Algisi V, Conte A, Raimondi A, Pasham M, Upadhyayula S, et al. Molecularly Distinct Clathrin-Coated Pits Differentially Impact EGFR Fate and Signaling. Cell Rep. 2019;27:3049-3061.e6 pubmed publisher
	Ghosh M, Lo R, Ivic I, Aguilera B, Qendro V, Devarakonda C, et al. CD13 tethers the IQGAP1-ARF6-EFA6 complex to the plasma membrane to promote ARF6 activation, β1 integrin recycling, and cell migration. Sci Signal. 2019;12: pubmed publisher
	Rodriguez Rodriguez J, Lewis C, McKinley K, Sikirzhytski V, Corona J, Maciejowski J, et al. Distinct Roles of RZZ and Bub1-KNL1 in Mitotic Checkpoint Signaling and Kinetochore Expansion. Curr Biol. 2018;28:3422-3429.e5 pubmed publisher
	Noethel B, Ramms L, Dreissen G, Hoffmann M, Springer R, Rübsam M, et al. Transition of responsive mechanosensitive elements from focal adhesions to adherens junctions on epithelial differentiation. Mol Biol Cell. 2018;29:2317-2325 pubmed publisher
	Qiang L, Sun X, Austin T, Muralidharan H, Jean D, Liu M, et al. Tau Does Not Stabilize Axonal Microtubules but Rather Enables Them to Have Long Labile Domains. Curr Biol. 2018;28:2181-2189.e4 pubmed publisher
	Xu X, Wicki Stordeur L, Sanchez Arias J, Liu M, Weaver M, Choi C, et al. Probenecid Disrupts a Novel Pannexin 1-Collapsin Response Mediator Protein 2 Interaction and Increases Microtubule Stability. Front Cell Neurosci. 2018;12:124 pubmed publisher
	Rhee S, Chung J, King D, D Amato G, Paik D, Duan A, et al. Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease. Nat Commun. 2018;9:368 pubmed publisher
	Halaoui R, Rejon C, Chatterjee S, Szymborski J, Meterissian S, Muller W, et al. Progressive polarity loss and luminal collapse disrupt tissue organization in carcinoma. Genes Dev. 2017;31:1573-1587 pubmed publisher
	Zhang J, Johnson J, He J, Napolitano G, Ramadass M, Rocca C, et al. Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A. J Biol Chem. 2017;292:10328-10346 pubmed publisher
	Verdeny Vilanova I, Wehnekamp F, Mohan N, Sandoval Ãlvarez Ã, Borbely J, Otterstrom J, et al. 3D motion of vesicles along microtubules helps them to circumvent obstacles in cells. J Cell Sci. 2017;130:1904-1916 pubmed publisher
	Ng B, Selvaraj G, Santa Cruz Mateos C, Grosheva I, Alvarez Garcia I, MartÃn Bermudo M, et al. Î±-Spectrin and integrins act together to regulate actomyosin and columnarization, and to maintain a monolayered follicular epithelium. Development. 2016;143:1388-99 pubmed publisher
	Denu R, Zasadil L, Kanugh C, Laffin J, Weaver B, Burkard M. Centrosome amplification induces high grade features and is prognostic of worse outcomes in breast cancer. BMC Cancer. 2016;16:47 pubmed publisher
	Eberhardt E, Havlicek S, Schmidt D, Link A, Neacsu C, Kohl Z, et al. Pattern of Functional TTX-Resistant Sodium Channels Reveals a Developmental Stage of Human iPSC- and ESC-Derived Nociceptors. Stem Cell Reports. 2015;5:305-13 pubmed publisher
	Ng D, Humphries J, Byron A, Millon FrÃ©millon A, Humphries M. Microtubule-dependent modulation of adhesion complex composition. PLoS ONE. 2014;9:e115213 pubmed publisher
	Gegg M, BÃ¶ttcher A, Burtscher I, Hasenoeder S, Van Campenhout C, Aichler M, et al. Flattop regulates basal body docking and positioning in mono- and multiciliated cells. elife. 2014;3: pubmed publisher
	Watanabe M, Serizawa M, Sawada T, Takeda K, Takahashi T, Yamamoto N, et al. A novel flow cytometry-based cell capture platform for the detection, capture and molecular characterization of rare tumor cells in blood. J Transl Med. 2014;12:143 pubmed publisher
	Lysko D, Putt M, Golden J. SDF1 reduces interneuron leading process branching through dual regulation of actin and microtubules. J Neurosci. 2014;34:4941-62 pubmed publisher
	Bugnicourt G, Brocard J, Nicolas A, Villard C. Nanoscale surface topography reshapes neuronal growth in culture. Langmuir. 2014;30:4441-9 pubmed publisher
	Maida Y, Yasukawa M, Okamoto N, Ohka S, Kinoshita K, Totoki Y, et al. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Mol Cell Biol. 2014;34:1576-93 pubmed publisher
	Gaggioli V, Zeiser E, Rivers D, Bradshaw C, Ahringer J, Zegerman P. CDK phosphorylation of SLD-2 is required for replication initiation and germline development in C. elegans. J Cell Biol. 2014;204:507-22 pubmed publisher
	Hell M, Thoma C, Fankhauser N, Christinat Y, Weber T, Krek W. miR-28-5p promotes chromosomal instability in VHL-associated cancers by inhibiting Mad2 translation. Cancer Res. 2014;74:2432-43 pubmed publisher
	Völler D, Reinders J, Meister G, Bosserhoff A. Strong reduction of AGO2 expression in melanoma and cellular consequences. Br J Cancer. 2013;109:3116-24 pubmed publisher
	Balogun F, Truman A, Kron S. DNA resection proteins Sgs1 and Exo1 are required for G1 checkpoint activation in budding yeast. DNA Repair (Amst). 2013;12:751-60 pubmed publisher
	Lera R, Burkard M. High mitotic activity of Polo-like kinase 1 is required for chromosome segregation and genomic integrity in human epithelial cells. J Biol Chem. 2012;287:42812-25 pubmed publisher
	Alves Silva J, SÃ¡nchez Soriano N, Beaven R, Klein M, Parkin J, Millard T, et al. Spectraplakins promote microtubule-mediated axonal growth by functioning as structural microtubule-associated proteins and EB1-dependent +TIPs (tip interacting proteins). J Neurosci. 2012;32:9143-58 pubmed publisher
	Preobrazhenska O, Wright J, Churg A. Regional heterogeneity in murine lung fibroblasts from normal mice or mice exposed once to cigarette smoke. PLoS ONE. 2012;7:e39761 pubmed publisher
	Kirby B, Jodari M, Loftus M, Gakhar G, Pratt E, Chanel Vos C, et al. Functional characterization of circulating tumor cells with a prostate-cancer-specific microfluidic device. PLoS ONE. 2012;7:e35976 pubmed publisher
	Lei W, Xing S, Deng C, Ju X, Jiang X, Luo Z. Laminin/?1 integrin signal triggers axon formation by promoting microtubule assembly and stabilization. Cell Res. 2012;22:954-72 pubmed publisher
	Mian I, Pierre Louis W, Dole N, Gilberti R, Dodge Kafka K, Tirnauer J. LKB1 destabilizes microtubules in myoblasts and contributes to myoblast differentiation. PLoS ONE. 2012;7:e31583 pubmed publisher
	Esson H, Morriswood B, Yavuz S, Vidilaseris K, Dong G, Warren G. Morphology of the trypanosome bilobe, a novel cytoskeletal structure. Eukaryot Cell. 2012;11:761-72 pubmed publisher
	Niediek V, Born S, Hampe N, Kirchgessner N, Merkel R, Hoffmann B. Cyclic stretch induces reorientation of cells in a Src family kinase- and p130Cas-dependent manner. Eur J Cell Biol. 2012;91:118-28 pubmed publisher
	Khoury H, Garcia Manero G, Borthakur G, Kadia T, Foudray M, Arellano M, et al. A phase 1 dose-escalation study of ARRY-520, a kinesin spindle protein inhibitor, in patients with advanced myeloid leukemias. Cancer. 2012;118:3556-64 pubmed publisher
	Hu X, Ballo L, Pietila L, Viesselmann C, Ballweg J, Lumbard D, et al. BDNF-induced increase of PSD-95 in dendritic spines requires dynamic microtubule invasions. J Neurosci. 2011;31:15597-603 pubmed publisher
	Bertossi A, Aichinger M, Sansonetti P, Lech M, Neff F, Pal M, et al. Loss of Roquin induces early death and immune deregulation but not autoimmunity. J Exp Med. 2011;208:1749-56 pubmed publisher
	Nakata T, Niwa S, Okada Y, Perez F, Hirokawa N. Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport. J Cell Biol. 2011;194:245-55 pubmed publisher
	Wang F, Kooistra M, Lee M, Liu L, Baltz J. Mouse embryos stressed by physiological levels of osmolarity become arrested in the late 2-cell stage before entry into M phase. Biol Reprod. 2011;85:702-13 pubmed publisher
	Zenner H, Yoshimura S, Barr F, Crump C. Analysis of Rab GTPase-activating proteins indicates that Rab1a/b and Rab43 are important for herpes simplex virus 1 secondary envelopment. J Virol. 2011;85:8012-21 pubmed publisher
	Lee S, Liu H, Lin W, Guo H, Lu B. LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction. J Neurosci. 2010;30:16959-69 pubmed publisher
	Brosig M, Ferralli J, Gelman L, Chiquet M, Chiquet Ehrismann R. Interfering with the connection between the nucleus and the cytoskeleton affects nuclear rotation, mechanotransduction and myogenesis. Int J Biochem Cell Biol. 2010;42:1717-28 pubmed publisher
	Schuhmann M, Stegner D, Berna Erro A, Bittner S, Braun A, Kleinschnitz C, et al. Stromal interaction molecules 1 and 2 are key regulators of autoreactive T cell activation in murine autoimmune central nervous system inflammation. J Immunol. 2010;184:1536-42 pubmed publisher
	Vodrazka P, Korostylev A, Hirschberg A, Swiercz J, Worzfeld T, Deng S, et al. The semaphorin 4D-plexin-B signalling complex regulates dendritic and axonal complexity in developing neurons via diverse pathways. Eur J Neurosci. 2009;30:1193-208 pubmed publisher
	Alves Silva J, Hahn I, Huber O, Mende M, Reissaus A, Prokop A. Prominent actin fiber arrays in Drosophila tendon cells represent architectural elements different from stress fibers. Mol Biol Cell. 2008;19:4287-97 pubmed publisher
	Nakaya Y, Sukowati E, Wu Y, Sheng G. RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation. Nat Cell Biol. 2008;10:765-75 pubmed publisher
	Amadio M, Scapagnini G, Lupo G, Drago F, Govoni S, Pascale A. PKCbetaII/HuR/VEGF: A new molecular cascade in retinal pericytes for the regulation of VEGF gene expression. Pharmacol Res. 2008;57:60-6 pubmed publisher
	Soper J, Roy S, Stieber A, Lee E, Wilson R, Trojanowski J, et al. Alpha-synuclein-induced aggregation of cytoplasmic vesicles in Saccharomyces cerevisiae. Mol Biol Cell. 2008;19:1093-103 pubmed publisher
	Montembault E, Dutertre S, Prigent C, Giet R. PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores. J Cell Biol. 2007;179:601-9 pubmed
	Bandyopadhyay B, Li G, Yin H, Kuret J. Tau aggregation and toxicity in a cell culture model of tauopathy. J Biol Chem. 2007;282:16454-64 pubmed
	Schaap I, Hoffmann B, Carrasco C, Merkel R, Schmidt C. Tau protein binding forms a 1 nm thick layer along protofilaments without affecting the radial elasticity of microtubules. J Struct Biol. 2007;158:282-92 pubmed
	Provenzani A, Fronza R, Loreni F, Pascale A, Amadio M, Quattrone A. Global alterations in mRNA polysomal recruitment in a cell model of colorectal cancer progression to metastasis. Carcinogenesis. 2006;27:1323-33 pubmed
	Cui W, Hawley R. The HhH2/NDD domain of the Drosophila Nod chromokinesin-like protein is required for binding to chromosomes in the oocyte nucleus. Genetics. 2005;171:1823-35 pubmed
	Marcus A, Peters U, Thomas S, Garrett S, Zelnak A, Kapoor T, et al. Mitotic kinesin inhibitors induce mitotic arrest and cell death in Taxol-resistant and -sensitive cancer cells. J Biol Chem. 2005;280:11569-77 pubmed
	Elena de Bellard M, Bronner Fraser M. Neural crest migration methods in the chicken embryo. Methods Mol Biol. 2005;294:247-67 pubmed

product information

Catalog Number :

MAB1864

Subcategory :

Cell Structure

Product Name :

Anti-Tubulin Antibody, clone YL1/2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q71U36

Host Name :

Rat

Antigen :

Tubulin

Clone :

YL1/2

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Tubulin Antibody, clone YL1/2

Cross Reactivity :

Human;Mouse;Rat

Background :

Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtubule formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.

Immunogen :

Yeast tubulin

Specificity :

MAB1864 recognizes the α subunit of tubulin-specificity binding to so called Tyr-Tubulin, which is produced by post-translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin.

Package Size :

100 µg

Uses :

ELISA;Immunohistochemistry;Immunoprecipitation;Radioimmunoassay;Western Blotting

Storage :

Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.