CD59 Antibody, clone 2/24 | EMD Millipore MAB1759 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

CD59 Antibody, clone 2/24

catalog :

MAB1759

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2/24

reactivity :

human

application :

immunocytochemistry, flow cytometry

citations: 8

Reference
Hagiwara K, Obayashi T, Sakayori N, Yamanishi E, Hayashi R, Osumi N, et al. Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells. PLoS ONE. 2014;9:e84072 pubmed publisher
Jadaliha M, Lee H, Pakzad M, Fathi A, Jeong S, Cho S, et al. Quantitative proteomic analysis of human embryonic stem cell differentiation by 8-plex iTRAQ labelling. PLoS ONE. 2012;7:e38532 pubmed publisher
Nishii T, Oikawa Y, Ishida Y, Kawaichi M, Matsuda E. CtBP-interacting BTB zinc finger protein (CIBZ) promotes proliferation and G1/S transition in embryonic stem cells via Nanog. J Biol Chem. 2012;287:12417-24 pubmed publisher
Abe M, Tsai S, Jin S, Pfeifer G, SZABO P. Sex-specific dynamics of global chromatin changes in fetal mouse germ cells. PLoS ONE. 2011;6:e23848 pubmed publisher
Nakamura K, Salomonis N, Tomoda K, Yamanaka S, Conklin B. G(i)-coupled GPCR signaling controls the formation and organization of human pluripotent colonies. PLoS ONE. 2009;4:e7780 pubmed publisher
Ahmadiankia N, Ebrahimi M, Hosseini A, Baharvand H. Effects of different extracellular matrices and co-cultures on human limbal stem cell expansion in vitro. Cell Biol Int. 2009;33:978-87 pubmed publisher
Baharvand H, Fathi A, Gourabi H, Mollamohammadi S, Salekdeh G. Identification of mouse embryonic stem cell-associated proteins. J Proteome Res. 2008;7:412-23 pubmed
Baharvand H, Ebrahimi M, Javadi M. Comparison of characteristics of cultured limbal cells on denuded amniotic membrane and fresh conjunctival, limbal and corneal tissues. Dev Growth Differ. 2007;49:241-51 pubmed

product information

Catalog Number :

MAB1759

Subcategory :

Inflammation & Immunology

Product Name :

Anti-CD59 Antibody, clone 2/24

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P13987

Host Name :

Mouse

Antigen :

CD59

Clone :

2/24

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-CD59 Antibody, clone 2/24

Cross Reactivity :

Human

ALT Names :

Protectin

Immunogen :

Human erythrocytes

Specificity :

This antibody binds to a 17-25kD glycoprotein (Fletcher et al., 1990a,b) which is distributed on human cells and has a glycosyl-phosphatidylinositol (GPI) linkage to the plasma membrane(Hadam, 1989). It is identical to other CD59 monoclonals, MEM-43 and YTH 53.1, defined at the 4th International Conference on Human Leucocyte Differentiation Antigens(Davies et al., 1989; Stefanova et al., 1989). CD59 is an inhibitor of attack by autologous complement(Ojcius et al., 1990) possibly by limiting incorporation of C9 into the membrane complex(Rollins & Sims, 1990; Meri et al., 1990). GPI linked proteins such as CD59 are deficient on cells from patients with the acquired clonal disorder paroxysmal nocturnal haemoglobulinuria (PNH) in which the erythrocytes are susceptible to lysis by complement and leading to anemia(Rosse, 1990). Evidence suggests that CD59 may be the most important complement regulatory protein missing in PNH(Fletcher et al., 1990; Yamashina et al., 1990). Cell reactivity (Fletcher et al., 1990a,b; Hadam, 1989): The antibody reacts with all leucocytes, platelets, fibroblasts, erythrocytes and most cell lines. It is negative on U937 and Daudi cell lines. It is reduced on affected cells from patients with PNH with a distribution reflecting the clonal nature of the disease.

Package Size :

100 µg

Uses :

Flow Cytometry;Immunofluorescence

Storage :

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES. WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.