Bim Antibody, clone 14A8 | EMD Millipore MAB17001-50UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Bim Antibody, clone 14A8

catalog :

MAB17001-50UG

quantity :

50 μg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

14A8

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000	Sosa Seda I, Mott J, Akazawa Y, Barreyro F, Bronk S, Kaufmann S, et al. Noxa mediates hepatic stellate cell apoptosis by proteasome inhibition. Hepatol Res. 2010;40:701-10 pubmed publisher
	Sale M, Cook S. The BH3 mimetic ABT-263 synergizes with the MEK1/2 inhibitor selumetinib/AZD6244 to promote BIM-dependent tumour cell death and inhibit acquired resistance. Biochem J. 2013;450:285-94 pubmed publisher
	Paterson A, Mockridge C, Adams J, Krysov S, Potter K, Duncombe A, et al. Mechanisms and clinical significance of BIM phosphorylation in chronic lymphocytic leukemia. Blood. 2012;119:1726-36 pubmed publisher
	Yamamura Y, Lee W, Inoue K, Ida H, Ito Y. RUNX3 cooperates with FoxO3a to induce apoptosis in gastric cancer cells. J Biol Chem. 2006;281:5267-76 pubmed

product information

Catalog Number :

MAB17001-50UG

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-Bim Antibody, clone 14A8

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

O43521

Host Name :

Rat

Antigen :

Bim

Clone :

14A8

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Bim Antibody, clone 14A8

Cross Reactivity :

Human;Mouse

Background :

Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: BimEL, BimL, and BimS. Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3. Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation. BimEL, the long isoform of Bim, is a ~28 kDa protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway. The activation of BimEL by ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.

Immunogen :

Recombinant mouse BimShort (BimS) protein.

Specificity :

Purified rat monoclonal antibody IgG2a Kappa isotype raised against recombinant mouse BimShort (BimS) protein. The antibody recognises a reactive epitope that is common to all splice variants of Bim including BimExtra Long, BimLong and BimShort. These antibodies can be used for the detection of Bim by standard techniques including Western blot, immunoprecipitation, immunohistochemistry and FACS. This antibody is highly sensitive and can, in most cases, recognize endogenous levels of BimS*.

Package Size :

50 μg

Uses :

Flow Cytometry;Immunohistochemistry;Immunoprecipitation;Western Blotting

Storage :

Maintain 6 months at 4°C, from date of shipment. Do not freeze. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.