MCAM Antibody, clone P1H12, FITC conjugated | EMD Millipore MAB16985F product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MCAM Antibody, clone P1H12, FITC conjugated

catalog :

MAB16985F

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

P1H12

The same clone is also sold as:

BioLegend: 361038, 361002, 361022, 361001, 361036, 361037, 361003, 361029, 361007, 361005, 361012, 361030, 361017, 361013, 361006, 361011, 361014, 361016, 361010, 361023, 361033, 361032, 361031, 361020, 361039, 361024, 361009, 361015, 361025, 361008, 361027, 361026, 361021, 361019, 361004, 361028
BD Biosciences: 560846, 561013, 562136, 550314, 562135, 550315
Abnova: MAB10893
Santa Cruz Biotechnology: sc-18837
Abcam: ab78451, ab24577
Invitrogen: MA1-25100, 25-1469-41, 14-1469-95, 50-1469-42, 14-1469-37, 12-1469-41, 25-1469-42, 11-1469-42, 14-1469-82, 12-1469-42, 48-1469-42, 11-1469-41
LifeSpan Biosciences: LS-B4050
OriGene: TA318345, TA320421
Exbio: 1P-783-T100, 11-783-C025, 11-783-C100, 1P-783-T025
Novus Biologicals: NBP1-43346-0.1mg

reactivity :

human, mouse

application :

flow cytometry

citations: 20

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:100; fig s1	Marques Howarth M, Simpson D, Ngok S, Nieves B, Chen R, Siprashvili Z, et al. Long noncoding RNA EWSAT1-mediated gene repression facilitates Ewing sarcoma oncogenesis. J Clin Invest. 2014;124:5275-90 pubmed publisher
flow cytometry; mouse; 1:20	Navone S, Marfia G, Nava S, Invernici G, Cristini S, Balbi S, et al. Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survival. Vasc Cell. 2013;5:10 pubmed publisher
	Stimpfel M, Cerkovnik P, Novakovic S, Maver A, Virant Klun I. Putative mesenchymal stem cells isolated from adult human ovaries. J Assist Reprod Genet. 2014;31:959-74 pubmed publisher
	Roubelakis M, Tsaknakis G, Pappa K, Anagnou N, Watt S. Spindle shaped human mesenchymal stem/stromal cells from amniotic fluid promote neovascularization. PLoS ONE. 2013;8:e54747 pubmed publisher
	Patel A, Vargas V, Revello P, Bull D. Mesenchymal stem cell population isolated from the subepithelial layer of umbilical cord tissue. Cell Transplant. 2013;22:513-9 pubmed publisher
	Guth A, Hafeman S, Elmslie R, Dow S. Liposomal clodronate treatment for tumour macrophage depletion in dogs with soft-tissue sarcoma. Vet Comp Oncol. 2013;11:296-305 pubmed publisher
	Irizarry Rovira A, Lynch S, David M, Ramos Vara J. Gonadoblastoma in the ovaries of a lesser galago (Galago senegalensis braccatus). J Comp Pathol. 2012;147:204-8 pubmed publisher
	Patel A, Yockman J, Vargas V, Bull D. Putative population of adipose-derived stem cells isolated from mediastinal tissue during cardiac surgery. Cell Transplant. 2013;22:507-11 pubmed publisher
	Sloboda N, Feve B, Thornton S, Nzietchueng R, Regnault V, Simon G, et al. Fatty acids impair endothelium-dependent vasorelaxation: a link between obesity and arterial stiffness in very old Zucker rats. J Gerontol A Biol Sci Med Sci. 2012;67:927-38 pubmed publisher
	Lapan A, Gussoni E. Isolation and characterization of human fetal myoblasts. Methods Mol Biol. 2012;798:3-19 pubmed publisher
	Agrawal V, Kelly J, Tottey S, Daly K, Johnson S, Siu B, et al. An isolated cryptic peptide influences osteogenesis and bone remodeling in an adult mammalian model of digit amputation. Tissue Eng Part A. 2011;17:3033-44 pubmed publisher
	Szoke K, Beckstrøm K, Brinchmann J. Human adipose tissue as a source of cells with angiogenic potential. Cell Transplant. 2012;21:235-50 pubmed publisher
	Ramalingam S, Belani C, Mack P, Vokes E, Longmate J, Govindan R, et al. Phase II study of Cediranib (AZD 2171), an inhibitor of the vascular endothelial growth factor receptor, for second-line therapy of small cell lung cancer (National Cancer Institute #7097). J Thorac Oncol. 2010;5:1279-84 pubmed publisher
	Dye D, Karlen S, Rohrbach B, Staub O, Braathen L, Eidne K, et al. hShroom1 links a membrane bound protein to the actin cytoskeleton. Cell Mol Life Sci. 2009;66:681-96 pubmed publisher
	Bagley R, Honma N, Weber W, Boutin P, Rouleau C, Shankara S, et al. Endosialin/TEM 1/CD248 is a pericyte marker of embryonic and tumor neovascularization. Microvasc Res. 2008;76:180-8 pubmed publisher
	Zhang Y, Fisher N, Newey S, Smythe J, Tatton L, Tsaknakis G, et al. The impact of proliferative potential of umbilical cord-derived endothelial progenitor cells and hypoxia on vascular tubule formation in vitro. Stem Cells Dev. 2009;18:359-75 pubmed publisher
	Scaffidi P, Misteli T. Lamin A-dependent misregulation of adult stem cells associated with accelerated ageing. Nat Cell Biol. 2008;10:452-9 pubmed publisher
	Schallmoser K, Bartmann C, Rohde E, Reinisch A, Kashofer K, Stadelmeyer E, et al. Human platelet lysate can replace fetal bovine serum for clinical-scale expansion of functional mesenchymal stromal cells. Transfusion. 2007;47:1436-46 pubmed
	Beaty R, Edwards J, Boon K, Siu I, Conway J, Riggins G. PLXDC1 (TEM7) is identified in a genome-wide expression screen of glioblastoma endothelium. J Neurooncol. 2007;81:241-8 pubmed
	Bagley R, Rouleau C, Morgenbesser S, Weber W, Cook B, Shankara S, et al. Pericytes from human non-small cell lung carcinomas: an attractive target for anti-angiogenic therapy. Microvasc Res. 2006;71:163-74 pubmed

product information

Catalog Number :

MAB16985F

Subcategory :

Cell Structure

Product Name :

Anti-MCAM Antibody, clone P1H12, FITC conjugated

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P43121

Host Name :

Mouse

Antigen :

MCAM

Clone :

P1H12

Conjugate :

FITC

Isotype :

IgG1

Product Description :

Anti-MCAM Antibody, clone P1H12, FITC conjugated

Cross Reactivity :

Canine;Human;Mouse

Background :

MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).

ALT Names :

MUC18; CD146

Immunogen :

Immunization of mice with HUVEC

Specificity :

P1H12 reacts specifically with CD146 {MCAM, MUC18}. In blood and marrow P1H12 reacts only with endothelial cells, and has been used to detect circulating endothelial cells in human, dogs and mouse samples. It positively stains normal, primary HUVEC and MVEC cultures and the endothelial cells of all vessels in normal frozen sections of human skin, intestine, ovary tonsil, lymph node, lung, and kidney. It does not stain carcinoma cell lines HT-29 and COLO205, and M21, the T cell lines Jurkat and HuT78, fibroblasts, HL-60 or CHO cells, or EBV-transformed B cell lines, although it expected that CD146 expression is present in many tumor lines. P1H12 does not stain normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain with P1H12 also are positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375.S2 are positive, while murine melanoma M3 is negative. One smooth muscle line (HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphoctes are negative with or without prior stimulation.

Package Size :

100 μg

Uses :

Flow Cytometry

Storage :

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.