Filamin A Antibody, clone PM6/317 | EMD Millipore MAB1678 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Filamin A Antibody, clone PM6/317

catalog :

MAB1678

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PM6/317

The same clone is also sold as:

MilliporeSigma: F6682
Santa Cruz Biotechnology: sc-58764
LifeSpan Biosciences: LS-C85122

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - free floating section

citations: 29

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:200 western blot; human; 1:10000	Szeto S, Williams E, Rudner A, Lee J. Phosphorylation of filamin A by Cdk1 regulates filamin A localization and daughter cell separation. Exp Cell Res. 2015;330:248-66 pubmed publisher
immunoprecipitation; human immunocytochemistry; human western blot; human	Uotila L, Jahan F, Soto Hinojosa L, Melandri E, GrÃ¶nholm M, Gahmberg C. Specific phosphorylations transmit signals from leukocyte Î²2 to Î²1 integrins and regulate adhesion. J Biol Chem. 2014;289:32230-42 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Jiang X, Yue J, Lu H, Campbell N, Yang Q, Lan S, et al. Inhibition of filamin-A reduces cancer metastatic potential. Int J Biol Sci. 2013;9:67-77 pubmed publisher
immunohistochemistry; human western blot; human	Reinstein E, Frentz S, Morgan T, Garcia Minaur S, Leventer R, McGillivray G, et al. Vascular and connective tissue anomalies associated with X-linked periventricular heterotopia due to mutations in Filamin A. Eur J Hum Genet. 2013;21:494-502 pubmed publisher
immunohistochemistry - free floating section; rat; 1:1,000 western blot; rat; 1:1,000	Noam Y, Phan L, McClelland S, Manders E, Ehrengruber M, Wadman W, et al. Distinct regional and subcellular localization of the actin-binding protein filamin A in the mature rat brain. J Comp Neurol. 2012;520:3013-34 pubmed publisher
	Jahan F, Madhavan S, Rolova T, Viazmina L, Grönholm M, Gahmberg C. Phosphorylation of the α-chain in the integrin LFA-1 enables β2-chain phosphorylation and α-actinin binding required for cell adhesion. J Biol Chem. 2018;293:12318-12330 pubmed publisher
	Chiang T, Wu H, Lee F. ADP-ribosylation factor-like 4C binding to filamin-A modulates filopodium formation and cell migration. Mol Biol Cell. 2017;28:3013-3028 pubmed publisher
	Nakamura F, Song M, Hartwig J, Stossel T. Documentation and localization of force-mediated filamin A domain perturbations in moving cells. Nat Commun. 2014;5:4656 pubmed publisher
	Ramakrishnan N, Drescher M, Khan K, Hatfield J, Drescher D. HCN1 and HCN2 proteins are expressed in cochlear hair cells: HCN1 can form a ternary complex with protocadherin 15 CD3 and F-actin-binding filamin A or can interact with HCN2. J Biol Chem. 2012;287:37628-46 pubmed publisher
	Bu Z, Liu R, Shang B, Cao Z, Pan Y, Zhou Q, et al. A monoclonal antibody SZ-117 that recognizes filamin A derived from tumor cells. Hybridoma (Larchmt). 2012;31:214-8 pubmed publisher
	Deng W, Lopez Camacho C, Tang J, Mendoza Villanueva D, Maya Mendoza A, Jackson D, et al. Cytoskeletal protein filamin A is a nucleolar protein that suppresses ribosomal RNA gene transcription. Proc Natl Acad Sci U S A. 2012;109:1524-9 pubmed publisher
	Najib S, Saint Laurent N, Esteve J, Schulz S, Boutet Robinet E, Fourmy D, et al. A switch of G protein-coupled receptor binding preference from phosphoinositide 3-kinase (PI3K)-p85 to filamin A negatively controls the PI3K pathway. Mol Cell Biol. 2012;32:1004-16 pubmed publisher
	Daniel P, Morgan T, Alanay Y, Bijlsma E, Cho T, Cole T, et al. Disease-associated mutations in the actin-binding domain of filamin B cause cytoplasmic focal accumulations correlating with disease severity. Hum Mutat. 2012;33:665-73 pubmed publisher
	Lu Q, Ding K, Frosch M, Jones S, Wolfe M, Xia W, et al. Alzheimer's disease-linked presenilin mutation (PS1M146L) induces filamin expression and ?-secretase independent redistribution. J Alzheimers Dis. 2010;22:235-45 pubmed publisher
	Inoue T, Pecen P, Maddala R, Skiba N, Pattabiraman P, Epstein D, et al. Characterization of cytoskeleton-enriched protein fraction of the trabecular meshwork and ciliary muscle cells. Invest Ophthalmol Vis Sci. 2010;51:6461-71 pubmed publisher
	Takabayashi T, Xie M, Takeuchi S, Kawasaki M, Yagi H, Okamoto M, et al. LL5beta directs the translocation of filamin A and SHIP2 to sites of phosphatidylinositol 3,4,5-triphosphate (PtdIns(3,4,5)P3) accumulation, and PtdIns(3,4,5)P3 localization is mutually modified by co-recruited SHIP2. J Biol Chem. 2010;285:16155-65 pubmed publisher
	Clark A, Sawyer G, Robertson S, Sutherland Smith A. Skeletal dysplasias due to filamin A mutations result from a gain-of-function mechanism distinct from allelic neurological disorders. Hum Mol Genet. 2009;18:4791-800 pubmed publisher
	David T, Strassel C, Eckly A, Cazenave J, Gachet C, Lanza F. The platelet glycoprotein GPIbbeta intracellular domain participates in von Willebrand factor induced-filopodia formation independently of the Ser 166 phosphorylation site. J Thromb Haemost. 2010;8:1077-87 pubmed publisher
	Takala H, Nurminen E, Nurmi S, Aatonen M, Strandin T, Takatalo M, et al. Beta2 integrin phosphorylation on Thr758 acts as a molecular switch to regulate 14-3-3 and filamin binding. Blood. 2008;112:1853-62 pubmed publisher
	Johansen L, Naumanen T, Knudsen A, Westerlund N, Gromova I, Junttila M, et al. IKAP localizes to membrane ruffles with filamin A and regulates actin cytoskeleton organization and cell migration. J Cell Sci. 2008;121:854-64 pubmed publisher
	Nurmi S, Gahmberg C, Fagerholm S. 14-3-3 proteins bind both filamin and alphaLbeta2 integrin in activated T cells. Ann N Y Acad Sci. 2006;1090:318-25 pubmed
	Mammoto A, Huang S, Ingber D. Filamin links cell shape and cytoskeletal structure to Rho regulation by controlling accumulation of p190RhoGAP in lipid rafts. J Cell Sci. 2007;120:456-67 pubmed
	Bachmann A, Howard J, Vogel C. Actin-binding protein filamin A is displayed on the surface of human neuroblastoma cells. Cancer Sci. 2006;97:1359-65 pubmed
	Nyman Huttunen H, Tian L, Ning L, Gahmberg C. alpha-Actinin-dependent cytoskeletal anchorage is important for ICAM-5-mediated neuritic outgrowth. J Cell Sci. 2006;119:3057-66 pubmed
	Ostapkowicz A, Inai K, Smith L, Kreda S, Spychala J. Lipid rafts remodeling in estrogen receptor-negative breast cancer is reversed by histone deacetylase inhibitor. Mol Cancer Ther. 2006;5:238-45 pubmed
	Yaroslavskiy B, Zhang Y, Kalla S, Garcia Palacios V, Sharrow A, Li Y, et al. NO-dependent osteoclast motility: reliance on cGMP-dependent protein kinase I and VASP. J Cell Sci. 2005;118:5479-87 pubmed
	Kim C, Cho Y, Kang C, Kim M, Lee H, Cho E, et al. Filamin is essential for shedding of the transmembrane serine protease, epithin. EMBO Rep. 2005;6:1045-51 pubmed
	Salasznyk R, Westcott A, Klees R, Ward D, Xiang Z, Vandenberg S, et al. Comparing the protein expression profiles of human mesenchymal stem cells and human osteoblasts using gene ontologies. Stem Cells Dev. 2005;14:354-66 pubmed
	Flanagan L, Chou J, Falet H, Neujahr R, Hartwig J, Stossel T. Filamin A, the Arp2/3 complex, and the morphology and function of cortical actin filaments in human melanoma cells. J Cell Biol. 2001;155:511-7 pubmed

product information

Catalog Number :

MAB1678

Subcategory :

Cell Structure

Product Name :

Anti-Filamin A Antibody, clone PM6/317

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P21333

Host Name :

Mouse

Antigen :

Filamin A

Clone :

PM6/317

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-Filamin A Antibody, clone PM6/317

Cross Reactivity :

Chicken;Guinea Pig;Human;Mouse;Rat;Rabbit

Background :

Filamin is a structural protein that forms flexible cross-links between two actin filaments. Filamin is a homodimer of polypeptide chains each joined to the other at one end with an actin binding site ath the other. It is present in smooth muscle, fibroblasts, platelets and lymphocytes.

ALT Names :

Alpha-Filamin;Filamin I;Endothelial Actin-binding Protein;ABP-280;Nonmuscle Filamin

Immunogen :

Human platelet protein.

Specificity :

Recognizes unprocessed, full length Human filamin (actin-binding protein; 270-280 kDa) as well as the 190 kDa N-terminal calpain cleavage fragment of filamin (Aakhus, 1992). Following induction of apoptosis in U937 cells, the antibody recognizes 170, 150, and 120 kDa N-terminal cleavage fragments of the full-length form presumably resulting from cleavage by activated caspase-3 (Umeda, 2001).

Package Size :

100 µL

Uses :

Immunofluorescence;Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.