This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Spectrin alpha chain (nonerythroid) Antibody, clone AA6
catalog :
MAB1622
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
AA6

The same clone is also sold as:
reactivity :
human, mouse, rat, chicken
application :
western blot, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - frozen section
citations: 91
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig 1d
Du T, Dewey J, Wagner E, Cui R, Heo J, Park J, et al. LMO7 deficiency reveals the significance of the cuticular plate for hearing function. Nat Commun. 2019;10:1117 pubmed publisher
  • western blot; human; fig 3
Kurbatskaya K, Phillips E, Croft C, Dentoni G, Hughes M, Wade M, et al. Upregulation of calpain activity precedes tau phosphorylation and loss of synaptic proteins in Alzheimer's disease brain. Acta Neuropathol Commun. 2016;4:34 pubmed publisher
  • western blot; mouse; fig s1
Korwitz A, Merkwirth C, Richter Dennerlein R, Tröder S, Sprenger H, Quirós P, et al. Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria. J Cell Biol. 2016;212:157-66 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; fig 8
Burns J, Kelly M, Hoa M, Morell R, Kelley M. Single-cell RNA-Seq resolves cellular complexity in sensory organs from the neonatal inner ear. Nat Commun. 2015;6:8557 pubmed publisher
  • western blot; rat; 1:1000
Curcio M, Salazar I, Inácio A, Duarte E, Canzoniero L, Duarte C. Brain ischemia downregulates the neuroprotective GDNF-Ret signaling by a calpain-dependent mechanism in cultured hippocampal neurons. Cell Death Dis. 2015;6:e1645 pubmed publisher
  • western blot; mouse; 1:500; fig 6
Altmeppen H, Prox J, Krasemann S, Puig B, Kruszewski K, Dohler F, et al. The sheddase ADAM10 is a potent modulator of prion disease. elife. 2015;4: pubmed publisher
  • immunoprecipitation; human
Hedgepeth S, Garcia M, Wagner L, Rodriguez A, Chintapalli S, Snyder R, et al. The BRCA1 tumor suppressor binds to inositol 1,4,5-trisphosphate receptors to stimulate apoptotic calcium release. J Biol Chem. 2015;290:7304-13 pubmed publisher
  • western blot; mouse
Maltecca F, Baseggio E, Consolato F, Mazza D, Podini P, Young S, et al. Purkinje neuron Ca2+ influx reduction rescues ataxia in SCA28 model. J Clin Invest. 2015;125:263-74 pubmed publisher
  • western blot; rat
Zang Y, Chen S, Liao G, Zhu H, Wei X, Cui Y, et al. Calpain-2 contributes to neuropathic pain following motor nerve injury via up-regulating interleukin-6 in DRG neurons. Brain Behav Immun. 2015;44:37-47 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Hempel C, Hoyer N, Kildemoes A, Jendresen C, Kurtzhals J. Systemic and Cerebral Vascular Endothelial Growth Factor Levels Increase in Murine Cerebral Malaria along with Increased Calpain and Caspase Activity and Can be Reduced by Erythropoietin Treatment. Front Immunol. 2014;5:291 pubmed publisher
  • western blot; rat
Fernandes J, Vieira M, Carreto L, Santos M, Duarte C, Carvalho A, et al. In vitro ischemia triggers a transcriptional response to down-regulate synaptic proteins in hippocampal neurons. PLoS ONE. 2014;9:e99958 pubmed publisher
  • western blot; human; 1:40000
Sánchez Osuna M, García Belinchón M, Iglesias Guimarais V, Gil Guiñón E, Casanelles E, Yuste V. Caspase-activated DNase is necessary and sufficient for oligonucleosomal DNA breakdown, but not for chromatin disassembly during caspase-dependent apoptosis of LN-18 glioblastoma cells. J Biol Chem. 2014;289:18752-69 pubmed publisher
  • western blot; mouse; 1:1000
Stein L, Wozniak D, Dearborn J, Kubota S, Apte R, Izumi Y, et al. Expression of Nampt in hippocampal and cortical excitatory neurons is critical for cognitive function. J Neurosci. 2014;34:5800-15 pubmed publisher
  • western blot; human
Navarro Garcia F, Serapio Palacios A, Vidal J, Salazar M, Tapia Pastrana G. EspC promotes epithelial cell detachment by enteropathogenic Escherichia coli via sequential cleavages of a cytoskeletal protein and then focal adhesion proteins. Infect Immun. 2014;82:2255-65 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Hinrichsen I, Ernst B, Nuber F, Passmann S, Schäfer D, Steinke V, et al. Reduced migration of MLH1 deficient colon cancer cells depends on SPTAN1. Mol Cancer. 2014;13:11 pubmed publisher
  • western blot; rat
Halpin L, Northrop N, Yamamoto B. Ammonia mediates methamphetamine-induced increases in glutamate and excitotoxicity. Neuropsychopharmacology. 2014;39:1031-8 pubmed publisher
  • western blot; mouse
Subramanian V, Moorleghen J, Balakrishnan A, Howatt D, Chishti A, Uchida H. Calpain-2 compensation promotes angiotensin II-induced ascending and abdominal aortic aneurysms in calpain-1 deficient mice. PLoS ONE. 2013;8:e72214 pubmed publisher
  • western blot; rat
Liu L, Martin R, Kohler G, Chan C. Palmitate induces transcriptional regulation of BACE1 and presenilin by STAT3 in neurons mediated by astrocytes. Exp Neurol. 2013;248:482-90 pubmed publisher
  • western blot; mouse; 1:5,000
Yoshioka H, Katsu M, Sakata H, Okami N, Wakai T, Kinouchi H, et al. The role of PARL and HtrA2 in striatal neuronal injury after transient global cerebral ischemia. J Cereb Blood Flow Metab. 2013;33:1658-65 pubmed publisher
  • western blot; human; 1:5000; fig 2
Park Y, Ko J, Jang Y, Kwon Y. Activation of AMP-activated protein kinase alleviates homocysteine-mediated neurotoxicity in SH-SY5Y cells. Neurochem Res. 2013;38:1561-71 pubmed publisher
  • western blot; rat; 1:5000
Huang Q, Wang H, Perry S, Figueiredo Pereira M. Negative regulation of 26S proteasome stability via calpain-mediated cleavage of Rpn10 subunit upon mitochondrial dysfunction in neurons. J Biol Chem. 2013;288:12161-74 pubmed publisher
  • western blot; rat; 1:10000
Barros Miñones L, Martín de Saavedra D, Perez Alvarez S, Orejana L, Suquía V, Goni Allo B, et al. Inhibition of calpain-regulated p35/cdk5 plays a central role in sildenafil-induced protection against chemical hypoxia produced by malonate. Biochim Biophys Acta. 2013;1832:705-17 pubmed publisher
  • western blot; mouse; 1:5000
Okami N, Narasimhan P, Yoshioka H, Sakata H, Kim G, Jung J, et al. Prevention of JNK phosphorylation as a mechanism for rosiglitazone in neuroprotection after transient cerebral ischemia: activation of dual specificity phosphatase. J Cereb Blood Flow Metab. 2013;33:106-14 pubmed publisher
  • western blot; rat; 1:1000
Papadeas S, Halloran C, McCown T, Breese G, Blake B. Changes in apical dendritic structure correlate with sustained ERK1/2 phosphorylation in medial prefrontal cortex of a rat model of dopamine D1 receptor agonist sensitization. J Comp Neurol. 2008;511:271-85 pubmed publisher
  • western blot; rat
Beier C, Wischhusen J, Gleichmann M, Gerhardt E, Pekanovic A, Krueger A, et al. FasL (CD95L/APO-1L) resistance of neurons mediated by phosphatidylinositol 3-kinase-Akt/protein kinase B-dependent expression of lifeguard/neuronal membrane protein 35. J Neurosci. 2005;25:6765-74 pubmed
Tower Gilchrist C, Zlatic S, Yu D, Chang Q, Wu H, Lin X, et al. Adaptor protein-3 complex is required for Vangl2 trafficking and planar cell polarity of the inner ear. Mol Biol Cell. 2019;:mbcE16080592 pubmed publisher
Wan L, Ren L, Chen L, Wang G, Liu X, Wang B, et al. M-Calpain Activation Facilitates Seizure Induced KCC2 Down Regulation. Front Mol Neurosci. 2018;11:287 pubmed publisher
Wang Q, He Q, Chen Y, Shao W, Yuan C, Wang Y. JNK-mediated microglial DICER degradation potentiates inflammatory responses to induce dopaminergic neuron loss. J Neuroinflammation. 2018;15:184 pubmed publisher
Lu F, Zhu J, Guo S, Wong B, Chehab F, Ferriero D, et al. Upregulation of cholesterol 24-hydroxylase following hypoxia-ischemia in neonatal mouse brain. Pediatr Res. 2018;83:1218-1227 pubmed publisher
Kiprowska M, Stepanova A, Todaro D, Galkin A, Haas A, Wilson S, et al. Neurotoxic mechanisms by which the USP14 inhibitor IU1 depletes ubiquitinated proteins and Tau in rat cerebral cortical neurons: Relevance to Alzheimer's disease. Biochim Biophys Acta Mol Basis Dis. 2017;1863:1157-1170 pubmed publisher
Russo R, Adornetto A, Cavaliere F, Varano G, Rusciano D, Morrone L, et al. Intravitreal injection of forskolin, homotaurine, and L-carnosine affords neuroprotection to retinal ganglion cells following retinal ischemic injury. Mol Vis. 2015;21:718-29 pubmed
Marsili S, Genini S, Sudharsan R, Gingrich J, Aguirre G, Beltran W. Exclusion of the unfolded protein response in light-induced retinal degeneration in the canine T4R RHO model of autosomal dominant retinitis pigmentosa. PLoS ONE. 2015;10:e0115723 pubmed publisher
Zhang P, Herbig U, Coffman F, Lambert M. Non-erythroid alpha spectrin prevents telomere dysfunction after DNA interstrand cross-link damage. Nucleic Acids Res. 2013;41:5321-40 pubmed publisher
Zhou H, Chen S, Byun H, Chen H, Li L, Han H, et al. N-methyl-D-aspartate receptor- and calpain-mediated proteolytic cleavage of K+-Cl- cotransporter-2 impairs spinal chloride homeostasis in neuropathic pain. J Biol Chem. 2012;287:33853-64 pubmed
Burns J, On D, Baker W, Collado M, Corwin J. Over half the hair cells in the mouse utricle first appear after birth, with significant numbers originating from early postnatal mitotic production in peripheral and striolar growth zones. J Assoc Res Otolaryngol. 2012;13:609-27 pubmed publisher
Wang C, Huang Y. Calpain 2 activated through N-methyl-D-aspartic acid receptor signaling cleaves CPEB3 and abrogates CPEB3-repressed translation in neurons. Mol Cell Biol. 2012;32:3321-32 pubmed publisher
Zhang F, Saha S, Kashina A. Arginylation-dependent regulation of a proteolytic product of talin is essential for cell-cell adhesion. J Cell Biol. 2012;197:819-36 pubmed publisher
Ramos Miguel A, García Sevilla J. Crosstalk between cdk5 and MEK-ERK signalling upon opioid receptor stimulation leads to upregulation of activator p25 and MEK1 inhibition in rat brain. Neuroscience. 2012;215:17-30 pubmed publisher
Gomes J, Costa J, Melo C, Felizzi F, Monteiro P, Pinto M, et al. Excitotoxicity downregulates TrkB.FL signaling and upregulates the neuroprotective truncated TrkB receptors in cultured hippocampal and striatal neurons. J Neurosci. 2012;32:4610-22 pubmed publisher
von Reyn C, Mott R, Siman R, Smith D, Meaney D. Mechanisms of calpain mediated proteolysis of voltage gated sodium channel ?-subunits following in vitro dynamic stretch injury. J Neurochem. 2012;121:793-805 pubmed publisher
Iglesias Guimarais V, Gil Guiñón E, Gabernet G, García Belinchón M, Sánchez Osuna M, Casanelles E, et al. Apoptotic DNA degradation into oligonucleosomal fragments, but not apoptotic nuclear morphology, relies on a cytosolic pool of DFF40/CAD endonuclease. J Biol Chem. 2012;287:7766-79 pubmed publisher
Morales Corraliza J, Berger J, Mazzella M, Veeranna -, Neubert T, Ghiso J, et al. Calpastatin modulates APP processing in the brains of ?-amyloid depositing but not wild-type mice. Neurobiol Aging. 2012;33:1125.e9-18 pubmed publisher
Siman R, Giovannone N, Toraskar N, Frangos S, Stein S, Levine J, et al. Evidence that a panel of neurodegeneration biomarkers predicts vasospasm, infarction, and outcome in aneurysmal subarachnoid hemorrhage. PLoS ONE. 2011;6:e28938 pubmed publisher
Briz V, Parkash J, Sánchez Redondo S, Prevot V, Suñol C. Allopregnanolone prevents dieldrin-induced NMDA receptor internalization and neurotoxicity by preserving GABA(A) receptor function. Endocrinology. 2012;153:847-60 pubmed publisher
Subramanian V, Uchida H, Ijaz T, Moorleghen J, Howatt D, Balakrishnan A. Calpain inhibition attenuates angiotensin II-induced abdominal aortic aneurysms and atherosclerosis in low-density lipoprotein receptor-deficient mice. J Cardiovasc Pharmacol. 2012;59:66-76 pubmed publisher
Shi Y, De Maria A, Wang H, Mathias R, FitzGerald P, Bassnett S. Further analysis of the lens phenotype in Lim2-deficient mice. Invest Ophthalmol Vis Sci. 2011;52:7332-9 pubmed publisher
Tu Y, Tsai Y, Wang L, Wu H, Huang C, Ho C. Overweight worsens apoptosis, neuroinflammation and blood-brain barrier damage after hypoxic ischemia in neonatal brain through JNK hyperactivation. J Neuroinflammation. 2011;8:40 pubmed publisher
Russo R, Berliocchi L, Adornetto A, Varano G, Cavaliere F, Nucci C, et al. Calpain-mediated cleavage of Beclin-1 and autophagy deregulation following retinal ischemic injury in vivo. Cell Death Dis. 2011;2:e144 pubmed publisher
Sato K, Minegishi S, Takano J, Plattner F, Saito T, Asada A, et al. Calpastatin, an endogenous calpain-inhibitor protein, regulates the cleavage of the Cdk5 activator p35 to p25. J Neurochem. 2011;117:504-15 pubmed publisher
Yang G, Liang B, Zhu J, Luo Z. Calpain activation by Wingless-type murine mammary tumor virus integration site family, member 5A (Wnt5a) promotes axonal growth. J Biol Chem. 2011;286:6566-76 pubmed publisher
Wu S, Arevalo J, Neubrand V, Zhang H, Arancio O, Chao M. The ankyrin repeat-rich membrane spanning (ARMS)/Kidins220 scaffold protein is regulated by activity-dependent calpain proteolysis and modulates synaptic plasticity. J Biol Chem. 2010;285:40472-8 pubmed publisher
Cassar S, Tovcimak A, Rustay N, Ellis T, Hooker B, Witte D, et al. Comparing levels of biochemical markers in CSF from cannulated and non-cannulated rats. J Neurosci Methods. 2010;192:249-53 pubmed publisher
Perez Alvarez S, Cuenca Lopez M, de Mera R, Puerta E, Karachitos A, Bednarczyk P, et al. Methadone induces necrotic-like cell death in SH-SY5Y cells by an impairment of mitochondrial ATP synthesis. Biochim Biophys Acta. 2010;1802:1036-47 pubmed publisher
Choy M, Chen M, Manikandan J, Peng Z, Jenner A, Melendez A, et al. Up-regulation of endoplasmic reticulum stress-related genes during the early phase of treatment of cultured cortical neurons by the proteasomal inhibitor lactacystin. J Cell Physiol. 2011;226:494-510 pubmed publisher
Nakajima T, Ochi S, Oda C, Ishii M, Ogawa K. Ischemic preconditioning attenuates of ischemia-induced degradation of spectrin and tau: implications for ischemic tolerance. Neurol Sci. 2011;32:229-39 pubmed publisher
Frederick J, Chen Z, Bevers M, Ingleton L, Ma M, Neumar R. Neuroprotection with delayed calpain inhibition after transient forebrain ischemia. Crit Care Med. 2008;36:S481-5 pubmed
Park Y, Jang Y, Kwon Y. Protective effect of isoflavones against homocysteine-mediated neuronal degeneration in SH-SY5Y cells. Amino Acids. 2010;39:785-94 pubmed publisher
Garcia Ratés S, Camarasa J, Sánchez García A, Gandia L, Escubedo E, Pubill D. The effects of 3,4-methylenedioxymethamphetamine (MDMA) on nicotinic receptors: intracellular calcium increase, calpain/caspase 3 activation, and functional upregulation. Toxicol Appl Pharmacol. 2010;244:344-53 pubmed publisher
Antic D, Stubbs J, Suyama K, Kintner C, Scott M, Axelrod J. Planar cell polarity enables posterior localization of nodal cilia and left-right axis determination during mouse and Xenopus embryogenesis. PLoS ONE. 2010;5:e8999 pubmed publisher
Puerta E, Hervias I, Barros Miñones L, Jordan J, Ricobaraza A, Cuadrado Tejedor M, et al. Sildenafil protects against 3-nitropropionic acid neurotoxicity through the modulation of calpain, CREB, and BDNF. Neurobiol Dis. 2010;38:237-45 pubmed publisher
Zadran S, Jourdi H, Rostamiani K, Qin Q, Bi X, Baudry M. Brain-derived neurotrophic factor and epidermal growth factor activate neuronal m-calpain via mitogen-activated protein kinase-dependent phosphorylation. J Neurosci. 2010;30:1086-95 pubmed publisher
Siman R, Toraskar N, Dang A, McNeil E, McGarvey M, Plaum J, et al. A panel of neuron-enriched proteins as markers for traumatic brain injury in humans. J Neurotrauma. 2009;26:1867-77 pubmed publisher
von Reyn C, Spaethling J, Mesfin M, Ma M, Neumar R, Smith D, et al. Calpain mediates proteolysis of the voltage-gated sodium channel alpha-subunit. J Neurosci. 2009;29:10350-6 pubmed publisher
Gerencser A, Mark K, Hubbard A, Divakaruni A, Mehrabian Z, Nicholls D, et al. Real-time visualization of cytoplasmic calpain activation and calcium deregulation in acute glutamate excitotoxicity. J Neurochem. 2009;110:990-1004 pubmed publisher
De Maria A, Shi Y, Kumar N, Bassnett S. Calpain expression and activity during lens fiber cell differentiation. J Biol Chem. 2009;284:13542-50 pubmed publisher
Martín Villar E, Yurrita M, Fernández Muñoz B, Quintanilla M, Renart J. Regulation of podoplanin/PA2.26 antigen expression in tumour cells. Involvement of calpain-mediated proteolysis. Int J Biochem Cell Biol. 2009;41:1421-9 pubmed publisher
Schreihofer D, Redmond L. Soy phytoestrogens are neuroprotective against stroke-like injury in vitro. Neuroscience. 2009;158:602-9 pubmed publisher
Briguet A, Erb M, Courdier Fruh I, Barzaghi P, Santos G, Herzner H, et al. Effect of calpain and proteasome inhibition on Ca2+-dependent proteolysis and muscle histopathology in the mdx mouse. FASEB J. 2008;22:4190-200 pubmed publisher
Cavaliere C, Cirillo G, Rosaria Bianco M, Rossi F, De Novellis V, Maione S, et al. Gliosis alters expression and uptake of spinal glial amino acid transporters in a mouse neuropathic pain model. Neuron Glia Biol. 2007;3:141-53 pubmed publisher
Furness D, Mahendrasingam S, Ohashi M, Fettiplace R, Hackney C. The dimensions and composition of stereociliary rootlets in mammalian cochlear hair cells: comparison between high- and low-frequency cells and evidence for a connection to the lateral membrane. J Neurosci. 2008;28:6342-53 pubmed publisher
Serra Pérez A, Verdaguer E, Planas A, Santalucia T. Glucose promotes caspase-dependent delayed cell death after a transient episode of oxygen and glucose deprivation in SH-SY5Y cells. J Neurochem. 2008;106:1237-47 pubmed publisher
Alvira D, Ferrer I, Gutierrez Cuesta J, Garcia Castro B, Pallas M, Camins A. Activation of the calpain/cdk5/p25 pathway in the girus cinguli in Parkinson's disease. Parkinsonism Relat Disord. 2008;14:309-13 pubmed
Lovekamp Swan T, Glendenning M, Schreihofer D. A high soy diet reduces programmed cell death and enhances bcl-xL expression in experimental stroke. Neuroscience. 2007;148:644-52 pubmed
Saito T, Konno T, Hosokawa T, Asada A, Ishiguro K, Hisanaga S. p25/cyclin-dependent kinase 5 promotes the progression of cell death in nucleus of endoplasmic reticulum-stressed neurons. J Neurochem. 2007;102:133-40 pubmed
Corasaniti M, Maiuolo J, Maida S, Fratto V, Navarra M, Russo R, et al. Cell signaling pathways in the mechanisms of neuroprotection afforded by bergamot essential oil against NMDA-induced cell death in vitro. Br J Pharmacol. 2007;151:518-29 pubmed
Schlezinger J, Emberley J, Bissonnette S, Sherr D. An L-tyrosine derivative and PPARgamma agonist, GW7845, activates a multifaceted caspase cascade in bone marrow B cells. Toxicol Sci. 2007;98:125-36 pubmed
Deans M, Antic D, Suyama K, Scott M, Axelrod J, Goodrich L. Asymmetric distribution of prickle-like 2 reveals an early underlying polarization of vestibular sensory epithelia in the inner ear. J Neurosci. 2007;27:3139-47 pubmed
Dreolini L, Takei F. Activation of LFA-1 by ionomycin is independent of calpain-mediated talin cleavage. Biochem Biophys Res Commun. 2007;356:207-12 pubmed
Montero M, Poulsen F, Noraberg J, Kirkeby A, van Beek J, Leist M, et al. Comparison of neuroprotective effects of erythropoietin (EPO) and carbamylerythropoietin (CEPO) against ischemia-like oxygen-glucose deprivation (OGD) and NMDA excitotoxicity in mouse hippocampal slice cultures. Exp Neurol. 2007;204:106-17 pubmed
Ayala Grosso C, Tam J, Roy S, Xanthoudakis S, Da Costa D, Nicholson D, et al. Caspase-3 cleaved spectrin colocalizes with neurofilament-immunoreactive neurons in Alzheimer's disease. Neuroscience. 2006;141:863-74 pubmed
Axelsson V, Holback S, Sjogren M, Gustafsson H, Forsby A. Gliotoxin induces caspase-dependent neurite degeneration and calpain-mediated general cytotoxicity in differentiated human neuroblastoma SH-SY5Y cells. Biochem Biophys Res Commun. 2006;345:1068-74 pubmed
Alvira D, Tajes M, Verdaguer E, Acuna Castroviejo D, Folch J, Camins A, et al. Inhibition of the cdk5/p25 fragment formation may explain the antiapoptotic effects of melatonin in an experimental model of Parkinson's disease. J Pineal Res. 2006;40:251-8 pubmed
West T, Atzeva M, Holtzman D. Caspase-3 deficiency during development increases vulnerability to hypoxic-ischemic injury through caspase-3-independent pathways. Neurobiol Dis. 2006;22:523-37 pubmed
Müller G, Geist M, Veng L, Willesen M, Johansen F, Leist M, et al. A role for mixed lineage kinases in granule cell apoptosis induced by cytoskeletal disruption. J Neurochem. 2006;96:1242-52 pubmed
Staszewski R, Yamamoto B. Methamphetamine-induced spectrin proteolysis in the rat striatum. J Neurochem. 2006;96:1267-76 pubmed
Shin D, Bae Y, Kim Han J, Lee J, Choi I, Son K, et al. Polyphenol amentoflavone affords neuroprotection against neonatal hypoxic-ischemic brain damage via multiple mechanisms. J Neurochem. 2006;96:561-72 pubmed
Calon F, Lim G, Morihara T, Yang F, Ubeda O, Salem N, et al. Dietary n-3 polyunsaturated fatty acid depletion activates caspases and decreases NMDA receptors in the brain of a transgenic mouse model of Alzheimer's disease. Eur J Neurosci. 2005;22:617-26 pubmed
Siman R, Zhang C, Roberts V, Pitts Kiefer A, Neumar R. Novel surrogate markers for acute brain damage: cerebrospinal fluid levels corrrelate with severity of ischemic neurodegeneration in the rat. J Cereb Blood Flow Metab. 2005;25:1433-44 pubmed
Tremper Wells B, Vallano M. Nuclear calpain regulates Ca2+-dependent signaling via proteolysis of nuclear Ca2+/calmodulin-dependent protein kinase type IV in cultured neurons. J Biol Chem. 2005;280:2165-75 pubmed
Methot N, Huang J, Coulombe N, Vaillancourt J, Rasper D, Tam J, et al. Differential efficacy of caspase inhibitors on apoptosis markers during sepsis in rats and implication for fractional inhibition requirements for therapeutics. J Exp Med. 2004;199:199-207 pubmed
Bantel H, Sinha B, Domschke W, Peters G, Schulze Osthoff K, Jänicke R. alpha-Toxin is a mediator of Staphylococcus aureus-induced cell death and activates caspases via the intrinsic death pathway independently of death receptor signaling. J Cell Biol. 2001;155:637-48 pubmed
product information
Catalog Number :
MAB1622
Subcategory :
Cell Structure
Product Name :
Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
Q13813
Host Name :
Mouse
Antigen :
Spectrin alpha chain (nonerythroid)
Clone :
AA6
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6
Cross Reactivity :
Chicken;Guinea Pig;Human;Mouse;Rat;Rabbit;Pig;Mammals
Background :
Non-erythroid Spectrin, also known as Fodrin, plays a role in stabilizing membrane structures, maintaining cell shape and linking the cytoskeleton to plasma membranes or intracellular vesicles. As one of the primary targets cleaved by activated caspases during apoptosis, the full-length ~240 kDa protein can be cleaved to yield an N-terminal product of ~150 kDa, and C-terminal products of ~120 and ~35 kDa.
ALT Names :
(ALPHA)II-SPECTRIN;Alpha-II spectrin;Fodrin alpha chain;Spectrin, non-erythroid alpha chain;non-erythrocytic spectrin alpha;spectrin, alpha, non-erythrocytic 1 (alpha-fodrin);Fodrin α
Immunogen :
Chicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation.
Specificity :
Specific for the 240, 150, and 120 kDa forms of alpha-spectrin/alpha-fodrin molecule of all mammalian nonerythroid cells and chicken alpha-spectrin.
Package Size :
50 µg
Uses :
Immunohistochemistry;Immunoprecipitation;Western Blotting;Chromatin Immunoprecipitation (ChIP)
Storage :
Stable for 12 months at 2-8°C from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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