Neurofilament 145 kDa Antibody, CT, clone 3H11 | EMD Millipore MAB1621 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Neurofilament 145 kDa Antibody, CT, clone 3H11

catalog :

MAB1621

quantity :

300 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3H11

The same clone is also sold as:

Abnova: MAB2310
Bio-Rad: MCA4752
Invitrogen: MA1-33723, MA1-2011
Rockland Immunochemicals: 912-301-D84
OriGene: TA309180
Enzo Life Sciences: ADI-NBA-140-E

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - free floating section

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:200; fig 1a	Yoshitomi Y, Ikeda T, Saito H, Yoshitake Y, Ishigaki Y, Hatta T, et al. JunB regulates angiogenesis and neurovascular parallel alignment in mouse embryonic skin. J Cell Sci. 2017;130:916-926 pubmed publisher
immunocytochemistry; mouse; 1:500; fig 1	Beaudet M, Yang Q, Cadau S, Blais M, Bellenfant S, Gros Louis F, et al. High yield extraction of pure spinal motor neurons, astrocytes and microglia from single embryo and adult mouse spinal cord. Sci Rep. 2015;5:16763 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:500	Moore S, Khalaj A, Yoon J, Patel R, Hannsun G, Yoo T, et al. Therapeutic laquinimod treatment decreases inflammation, initiates axon remyelination, and improves motor deficit in a mouse model of multiple sclerosis. Brain Behav. 2013;3:664-82 pubmed publisher
	Thomas A, Seidlits S, Goodman A, Kukushliev T, Hassani D, Cummings B, et al. Sonic hedgehog and neurotrophin-3 increase oligodendrocyte numbers and myelination after spinal cord injury. Integr Biol (Camb). 2014;6:694-705 pubmed publisher
	Pérez De Sevilla Müller L, Sargoy A, Rodriguez A, Brecha N. Melanopsin ganglion cells are the most resistant retinal ganglion cell type to axonal injury in the rat retina. PLoS ONE. 2014;9:e93274 pubmed publisher
	Sargoy A, Sun X, Barnes S, Brecha N. Differential calcium signaling mediated by voltage-gated calcium channels in rat retinal ganglion cells and their unmyelinated axons. PLoS ONE. 2014;9:e84507 pubmed publisher
	Enomoto M, Bunge M, Tsoulfas P. A multifunctional neurotrophin with reduced affinity to p75NTR enhances transplanted Schwann cell survival and axon growth after spinal cord injury. Exp Neurol. 2013;248:170-82 pubmed publisher
	Thomas A, Shea L. Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury. J Control Release. 2013;170:421-9 pubmed publisher
	Thomas A, Kubilius M, Holland S, Seidlits S, Boehler R, Anderson A, et al. Channel density and porosity of degradable bridging scaffolds on axon growth after spinal injury. Biomaterials. 2013;34:2213-20 pubmed publisher
	Treiber C, Salzer M, Riegler J, Edelman N, Sugar C, Breuss M, et al. Clusters of iron-rich cells in the upper beak of pigeons are macrophages not magnetosensitive neurons. Nature. 2012;484:367-70 pubmed publisher
	Rieker C, Dev K, Lehnhoff K, Barbieri S, Ksiazek I, Kauffmann S, et al. Neuropathology in mice expressing mouse alpha-synuclein. PLoS ONE. 2011;6:e24834 pubmed publisher
	Yoshimura K, Sugiura K, Ohmori Y, Aste N, Saito N. Immunolocalization of aquaporin-4 in the brain, kidney, skeletal muscle, and gastro-intestinal tract of chicken. Cell Tissue Res. 2011;344:51-61 pubmed publisher
	Gunnersen J, Kuek A, Phipps J, Hammond V, Puthussery T, Fletcher E, et al. Seizure-related gene 6 (Sez-6) in amacrine cells of the rodent retina and the consequence of gene deletion. PLoS ONE. 2009;4:e6546 pubmed publisher
	Lain E, Carnejac S, Escher P, Wilson M, Lømo T, Gajendran N, et al. A novel role for embigin to promote sprouting of motor nerve terminals at the neuromuscular junction. J Biol Chem. 2009;284:8930-9 pubmed publisher
	Goto A, Wang Y, Kabuta T, Setsuie R, Osaka H, Sawa A, et al. Proteomic and histochemical analysis of proteins involved in the dying-back-type of axonal degeneration in the gracile axonal dystrophy (gad) mouse. Neurochem Int. 2009;54:330-8 pubmed publisher
	Jarjour A, Bull S, Almasieh M, Rajasekharan S, Baker K, Mui J, et al. Maintenance of axo-oligodendroglial paranodal junctions requires DCC and netrin-1. J Neurosci. 2008;28:11003-14 pubmed publisher
	Nyberg T, Shimada A, Torimitsu K. Ion conducting polymer microelectrodes for interfacing with neural networks. J Neurosci Methods. 2007;160:16-25 pubmed

product information

Catalog Number :

MAB1621

Subcategory :

Neuroscience

Product Name :

Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P07197

Host Name :

Mouse

Antigen :

Neurofilament 145 kDa

Clone :

3H11

Conjugate :

Culture Supernatant

Isotype :

IgG1

Product Description :

Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11

Cross Reactivity :

Avian;Mammals

Background :

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

Immunogen :

Recombinant fusion protein containing the last 168 amino acids of rat NF-M. Purified from E. Coli inclusion bodies by ion exchange chromatography on DEAE-cellulose in 6 M urea.

Specificity :

Reacts with an epitope dependent on the last 84 amino acids of rat NF-M (145K). Reacts specifically with NF-M from a variety of mammalian and avian species. The antibody is not sensitive to the phosphorylation state of the neurofilament.

Package Size :

300 µL

Uses :

Western Blotting;Immunocytochemistry;Immunohistochemistry

Storage :

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.