Cytokeratin 5 Antibody, 6, clone D5/16B4 | EMD Millipore MAB1620 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Cytokeratin 5 Antibody, 6, clone D5/16B4

catalog :

MAB1620

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

D5/16B4

The same clone is also sold as:

OriGene: TA327667

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

citations: 20

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:200; fig 2	Yamano S, Gi M, Tago Y, Doi K, Okada S, Hirayama Y, et al. Role of deltaNp63(pos)CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas. Cancer Sci. 2016;107:123-32 pubmed publisher
immunohistochemistry - paraffin section; human; 1:400	McCart Reed A, Kutasovic J, Vargas A, Jayanthan J, Al Murrani A, Reid L, et al. An epithelial to mesenchymal transition programme does not usually drive the phenotype of invasive lobular carcinomas. J Pathol. 2016;238:489-94 pubmed publisher
immunohistochemistry; human; 1:400	Winczura P, SosiÅ„ska Mielcarek K, Duchnowska R, Badzio A, Lakomy J, Majewska H, et al. Immunohistochemical Predictors of Bone Metastases in Breast Cancer Patients. Pathol Oncol Res. 2015;21:1229-36 pubmed publisher
	Somerville T, Xu Y, Miyabayashi K, Tiriac H, Cleary C, Maia Silva D, et al. TP63-Mediated Enhancer Reprogramming Drives the Squamous Subtype of Pancreatic Ductal Adenocarcinoma. Cell Rep. 2018;25:1741-1755.e7 pubmed publisher
	Offenbacher S, Jiao Y, Kim S, Marchesan J, Moss K, Jing L, et al. GWAS for Interleukin-1β levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation. Nat Commun. 2018;9:3686 pubmed publisher
	Jang W, Chen X, Flis D, Harris M, Schwob J. Label-retaining, quiescent globose basal cells are found in the olfactory epithelium. J Comp Neurol. 2014;522:731-49 pubmed publisher
	Tago Y, Yamano S, Wei M, Kakehashi A, Kitano M, Fujioka M, et al. Novel medium-term carcinogenesis model for lung squamous cell carcinoma induced by N-nitroso-tris-chloroethylurea in mice. Cancer Sci. 2013;104:1560-6 pubmed publisher
	Butler R, Spector M, Thomas D, MCDANIEL A, McHugh J. An immunohistochemical panel for reliable differentiation of salivary duct carcinoma and mucoepidermoid carcinoma. Head Neck Pathol. 2014;8:133-40 pubmed publisher
	Sosińska Mielcarek K, Duchnowska R, Winczura P, Badzio A, Majewska H, Lakomy J, et al. Immunohistochemical prediction of brain metastases in patients with advanced breast cancer: the role of Rad51. Breast. 2013;22:1178-83 pubmed publisher
	Senkus E, Szade J, Pieczyńska B, Zaczek A, Brozek I, Radecka B, et al. Are bilateral breast cancers different from breast cancers coexisting with ovarian cancer? An immunohistochemical analysis aimed at intrinsic tumor phenotype. Breast. 2013;22:425-30 pubmed publisher
	Allende D, Dumot J, Yerian L. Esophageal squamous cell carcinoma arising after endoscopic ablation therapy of Barrett's esophagus with high-grade dysplasia. Report of a case. Dis Esophagus. 2013;26:314-8 pubmed publisher
	Lott Limbach A, Hoschar A, Thompson L, Stelow E, Chute D. Middle ear adenomas stain for two cell populations and lack myoepithelial cell differentiation. Head Neck Pathol. 2012;6:345-53 pubmed publisher
	De Silva Rudland S, Platt Higgins A, Winstanley J, Jones N, Barraclough R, West C, et al. Statistical association of basal cell keratins with metastasis-inducing proteins in a prognostically unfavorable group of sporadic breast cancers. Am J Pathol. 2011;179:1061-72 pubmed publisher
	Holbrook E, Wu E, Curry W, Lin D, Schwob J. Immunohistochemical characterization of human olfactory tissue. Laryngoscope. 2011;121:1687-701 pubmed publisher
	Bao L, Haque A, Jackson K, Hazari S, Moroz K, Jetly R, et al. Increased expression of P-glycoprotein is associated with doxorubicin chemoresistance in the metastatic 4T1 breast cancer model. Am J Pathol. 2011;178:838-52 pubmed publisher
	Geyer F, Kushner Y, Lambros M, Natrajan R, Mackay A, Tamber N, et al. Microglandular adenosis or microglandular adenoma? A molecular genetic analysis of a case associated with atypia and invasive carcinoma. Histopathology. 2009;55:732-43 pubmed publisher
	Long K, Hornick J. SOX2 is highly expressed in squamous cell carcinomas of the gastrointestinal tract. Hum Pathol. 2009;40:1768-73 pubmed publisher
	Sohsman M, Yang H, Cassarino D. Sinonasal undifferentiated carcinoma metastatic to the skin. J Cutan Pathol. 2010;37:1241-4 pubmed publisher
	Skala C, Petry I, Gebhard S, Hengstler J, Albrich S, Maltaris T, et al. Isolation of fibroblasts for coating of meshes for reconstructive surgery: differences between mesh types. Regen Med. 2009;4:197-204 pubmed publisher
	Laczko I, Hudson D, Freeman A, Feneley M, Masters J. Comparison of the zones of the human prostate with the seminal vesicle: morphology, immunohistochemistry, and cell kinetics. Prostate. 2005;62:260-6 pubmed

product information

Catalog Number :

MAB1620

Subcategory :

Cell Structure

Product Name :

Anti-Cytokeratin 5 Antibody, 6, clone D5/16B4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P13647

Host Name :

Mouse

Antigen :

Cytokeratin 5

Clone :

D5/16B4

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Cytokeratin 5 Antibody, 6, clone D5/16B4

Cross Reactivity :

Human;Mouse;Rat

Background :

The cytokeratins are a family of intermediate filaments found in almost all epithelial cells. They are divided into four subclasses depending on their patterns of reactivity and electrophoretic characteristics and are also arranged I a numbered series of 20 or more acoring to molecular weight and isoelectric point (Quinlan et al. 1985). Types 5 and 6 of cytokeratin (CK5/6) are in high frequency in mesothelial cells, as opposed to cells of adenocarcinoma. CK5/6 has been shown to be a reliable marker for mesothelioma and squamous cell carcinoma of the lung and Bowen's disease. It does not react with pulmonary adenocarcinoma.

ALT Names :

58 kDa cytokeratin;epidermolysis bullosa simplex;Dowling-Meara/Kobner/Weber-Cockayne types;keratin 5;keratin 5 (epidermolysis bullosa simplex,Dowling-Meara/Kobner/Weber-Cockayne types);keratin, type II cytoskeletal 5

Immunogen :

Purified cytokeratin 5 (Exp. Cell Res. (1986) 162:114; J. Invest. Dermatology (1987) 88:191).

Specificity :

Reacts with human cytokeratin 5 on immunoblots of cytoskeletal preparations of epidermis and non-cytokeratinizing epithelium (Abstract: European Symposium of the Biology of the Cytoskeleton, Helsinki, 18-21, 6, 89, 1989) and on tissue sections. Shows reactivity with cytokeratin 6 and weak reactivity with cytokeratin 4 on immunoblots. No cross-reactivity with cytokeratins 1, 7, 8, 10, 13, 14, 18 or 19. MAB1620 recognizes basal cells and a part of the stratum spinosum in the normal pavement epithelium. The antibody may be useful for distinction of low differentiated pavement epithelium carcinoma and adenocarcinoma. The antibody works on formalin fixed tissues.

Package Size :

50 µg

Uses :

Immunofluorescence;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.