This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9
catalog :
MAB1596
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
6G6-1C9

The same clone is also sold as:
reactivity :
hamsters, African green monkey, zebra finch, mouse, rat, bovine, zebrafish , rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 76
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:100; loading ...; fig 7a
  • immunohistochemistry - frozen section; rat; 1:100; loading ...; fig 11f
  • immunocytochemistry; rat; 1:100; loading ...; fig 8h
Miralles C, Taylor M, Bear J, Fekete C, George S, Li Y, et al. Expression of protocadherin-γC4 protein in the rat brain. J Comp Neurol. 2019;: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2g
Salazar S, Cox T, Lee S, Brody A, Chyung A, Haas L, et al. Alzheimer's Disease Risk Factor Pyk2 Mediates Amyloid-β-Induced Synaptic Dysfunction and Loss. J Neurosci. 2019;39:758-772 pubmed publisher
  • immunohistochemistry - frozen section; zebra finch; 1:1000; loading ...; fig 2a
Ikeda M, Krentzel A, Oliver T, Scarpa G, Remage Healey L. Clustered organization and region-specific identities of estrogen-producing neurons in the forebrain of Zebra Finches (Taeniopygia guttata). J Comp Neurol. 2017;525:3636-3652 pubmed publisher
  • western blot; mouse; loading ...; tbl 1
Cao M, Wu Y, Ashrafi G, McCartney A, Wheeler H, Bushong E, et al. Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses and Triggers Dystrophic Changes in Dopaminergic Axons. Neuron. 2017;93:882-896.e5 pubmed publisher
  • immunohistochemistry - frozen section; zebrafish ; 1:500; loading ...; tbl 1
Yan Q, Zhai L, Zhang B, Dallman J. Spatial patterning of excitatory and inhibitory neuropil territories during spinal circuit development. J Comp Neurol. 2017;525:1649-1667 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4b
Van Hummel A, Bi M, Ippati S, van der Hoven J, Volkerling A, Lee W, et al. No Overt Deficits in Aged Tau-Deficient C57Bl/6.Mapttm1(EGFP)Kit GFP Knockin Mice. PLoS ONE. 2016;11:e0163236 pubmed publisher
  • western blot; rat; 1:100; loading ...; fig s1
Torres Vega E, Duran Moreno M, Sánchez del Pino M, Yañez Y, Canete A, Castel V, et al. Immunoproteomic studies on paediatric opsoclonus-myoclonus associated with neuroblastoma. J Neuroimmunol. 2016;297:98-102 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:1000; loading ...; fig 7e
  • western blot; rat; 1:1000; loading ...; fig 6f
Zhao T, Li C, Wei W, Zhang H, Ma D, Song X, et al. Prenatal ketamine exposure causes abnormal development of prefrontal cortex in rat. Sci Rep. 2016;6:26865 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1a
Hong S, Beja Glasser V, Nfonoyim B, Frouin A, Li S, Ramakrishnan S, et al. Complement and microglia mediate early synapse loss in Alzheimer mouse models. Science. 2016;352:712-716 pubmed publisher
  • western blot; hamsters; 1:500; fig 5
Bullmann T, Seeger G, Stieler J, Hanics J, Reimann K, Kretzschmann T, et al. Tau phosphorylation-associated spine regression does not impair hippocampal-dependent memory in hibernating golden hamsters. Hippocampus. 2016;26:301-18 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; fig 10A
Pérez de Sevilla Müller L, Sargoy A, Fernández Sánchez L, Rodriguez A, Liu J, Cuenca N, et al. Expression and cellular localization of the voltage-gated calcium channel α2δ3 in the rodent retina. J Comp Neurol. 2015;523:1443-60 pubmed publisher
  • western blot; rat; 1:1000; fig 1
Garcia Alvarez G, Lu B, Yap K, Wong L, Thevathasan J, Lim L, et al. STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs. Mol Biol Cell. 2015;26:1141-59 pubmed publisher
  • immunohistochemistry; rat
Nakano Kobayashi A, Tai Y, Nadif Kasri N, Van Aelst L. The X-linked mental retardation protein OPHN1 interacts with Homer1b/c to control spine endocytic zone positioning and expression of synaptic potentiation. J Neurosci. 2014;34:8665-71 pubmed publisher
  • immunocytochemistry; mouse
Nieland T, Logan D, Saulnier J, Lam D, Johnson C, Root D, et al. High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons. PLoS ONE. 2014;9:e91744 pubmed publisher
  • western blot; rhesus macaque; 1:5000
Willard S, Hemby S, Register T, McIntosh S, Shively C. Altered expression of glial and synaptic markers in the anterior hippocampus of behaviorally depressed female monkeys. Neurosci Lett. 2014;563:1-5 pubmed publisher
  • immunohistochemistry; mouse
  • western blot; mouse
  • western blot; African green monkey
Perlson E, Hendricks A, Lazarus J, Ben Yaakov K, Gradus T, Tokito M, et al. Dynein interacts with the neural cell adhesion molecule (NCAM180) to tether dynamic microtubules and maintain synaptic density in cortical neurons. J Biol Chem. 2013;288:27812-24 pubmed publisher
  • western blot; mouse; 1:500
Prox J, Bernreuther C, Altmeppen H, Grendel J, Glatzel M, D Hooge R, et al. Postnatal disruption of the disintegrin/metalloproteinase ADAM10 in brain causes epileptic seizures, learning deficits, altered spine morphology, and defective synaptic functions. J Neurosci. 2013;33:12915-28, 12928a pubmed publisher
  • immunocytochemistry; mouse; 1:100
  • immunohistochemistry; mouse
  • western blot; mouse; 1:2000
Eagleson K, Milner T, Xie Z, Levitt P. Synaptic and extrasynaptic location of the receptor tyrosine kinase met during postnatal development in the mouse neocortex and hippocampus. J Comp Neurol. 2013;521:3241-59 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:1000
  • immunohistochemistry - paraffin section; mouse; 1:1,000
de Sevilla Müller L, Liu J, Solomon A, Rodriguez A, Brecha N. Expression of voltage-gated calcium channel ?(2)?(4) subunits in the mouse and rat retina. J Comp Neurol. 2013;521:2486-501 pubmed publisher
Hansson O, Svensson M, Gustavsson A, Andersson E, Yang Y, Nagga K, et al. Midlife physical activity is associated with lower incidence of vascular dementia but not Alzheimer's disease. Alzheimers Res Ther. 2019;11:87 pubmed publisher
Rimbault C, Maruthi K, Breillat C, Genuer C, Crespillo S, Puente Muñoz V, et al. Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules. Nat Commun. 2019;10:4521 pubmed publisher
Edfawy M, Guedes J, Pereira M, Laranjo M, Carvalho M, Gao X, et al. Abnormal mGluR-mediated synaptic plasticity and autism-like behaviours in Gprasp2 mutant mice. Nat Commun. 2019;10:1431 pubmed publisher
de Pins B, Cifuentes Diaz C, Farah A, López Molina L, Montalban E, Sancho Balsells A, et al. Conditional BDNF Delivery from Astrocytes Rescues Memory Deficits, Spine Density, and Synaptic Properties in the 5xFAD Mouse Model of Alzheimer Disease. J Neurosci. 2019;39:2441-2458 pubmed publisher
Lee S, Salazar S, Cox T, Strittmatter S. Pyk2 Signaling through Graf1 and RhoA GTPase Is Required for Amyloid-β Oligomer-Triggered Synapse Loss. J Neurosci. 2019;39:1910-1929 pubmed publisher
Purkey A, Woolfrey K, Crosby K, Stich D, Chick W, Aoto J, et al. AKAP150 Palmitoylation Regulates Synaptic Incorporation of Ca2+-Permeable AMPA Receptors to Control LTP. Cell Rep. 2018;25:974-987.e4 pubmed publisher
Nestor J, Arinuma Y, Huerta T, Kowal C, Nasiri E, Kello N, et al. Lupus antibodies induce behavioral changes mediated by microglia and blocked by ACE inhibitors. J Exp Med. 2018;215:2554-2566 pubmed publisher
Krasovska V, Doering L. Regulation of IL-6 Secretion by Astrocytes via TLR4 in the Fragile X Mouse Model. Front Mol Neurosci. 2018;11:272 pubmed publisher
Nie X, Kitaoka S, Tanaka K, Segi Nishida E, Imoto Y, Ogawa A, et al. The Innate Immune Receptors TLR2/4 Mediate Repeated Social Defeat Stress-Induced Social Avoidance through Prefrontal Microglial Activation. Neuron. 2018;99:464-479.e7 pubmed publisher
Lee J, Han S, Park M, Baek B, Song I, Choi M, et al. Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease. Nat Commun. 2018;9:1479 pubmed publisher
Zhang Y, Zhu Z, Liang H, Zhang L, Zhou Q, Ni H, et al. nNOS-CAPON interaction mediates amyloid-?-induced neurotoxicity, especially in the early stages. Aging Cell. 2018;17:e12754 pubmed publisher
Chen H, Xing Y, Xia L, Chen Z, Yin S, Wang J. AAV-mediated NT-3 overexpression protects cochleae against noise-induced synaptopathy. Gene Ther. 2018;25:251-259 pubmed publisher
Zhou L, Zhou L, Su L, Cao S, Xie Y, Wang N, et al. Celecoxib Ameliorates Seizure Susceptibility in Autosomal Dominant Lateral Temporal Epilepsy. J Neurosci. 2018;38:3346-3357 pubmed publisher
Poirel O, Mella S, Videau C, Ramet L, Davoli M, Herzog E, et al. Moderate decline in select synaptic markers in the prefrontal cortex (BA9) of patients with Alzheimer's disease at various cognitive stages. Sci Rep. 2018;8:938 pubmed publisher
Paolicelli R, Jawaid A, Henstridge C, Valeri A, Merlini M, Robinson J, et al. TDP-43 Depletion in Microglia Promotes Amyloid Clearance but Also Induces Synapse Loss. Neuron. 2017;95:297-308.e6 pubmed publisher
Latina V, Caioli S, Zona C, Ciotti M, Amadoro G, Calissano P. Impaired NGF/TrkA Signaling Causes Early AD-Linked Presynaptic Dysfunction in Cholinergic Primary Neurons. Front Cell Neurosci. 2017;11:68 pubmed publisher
Sinnen B, Bowen A, Forte J, Hiester B, Crosby K, Gibson E, et al. Optogenetic Control of Synaptic Composition and Function. Neuron. 2017;93:646-660.e5 pubmed publisher
Valkova C, Liebmann L, Kramer A, Hübner C, Kaether C. The sorting receptor Rer1 controls Purkinje cell function via voltage gated sodium channels. Sci Rep. 2017;7:41248 pubmed publisher
Shen Y, Qin H, Chen J, Mou L, He Y, Yan Y, et al. Postnatal activation of TLR4 in astrocytes promotes excitatory synaptogenesis in hippocampal neurons. J Cell Biol. 2016;215:719-734 pubmed
Buesa I, Aira Z, Azkue J. Regulation of Nociceptive Plasticity Threshold and DARPP-32 Phosphorylation in Spinal Dorsal Horn Neurons by Convergent Dopamine and Glutamate Inputs. PLoS ONE. 2016;11:e0162416 pubmed publisher
Cécyre B, Monette M, Beudjekian L, Casanova C, Bouchard J. Localization of diacylglycerol lipase alpha and monoacylglycerol lipase during postnatal development of the rat retina. Front Neuroanat. 2014;8:150 pubmed publisher
Galic M, Tsai F, Collins S, Matis M, Bandara S, Meyer T. Dynamic recruitment of the curvature-sensitive protein ArhGAP44 to nanoscale membrane deformations limits exploratory filopodia initiation in neurons. elife. 2014;3:e03116 pubmed publisher
Xu J, Kam C, Luo J, Xia J. PICK1 mediates synaptic recruitment of AMPA receptors at neurexin-induced postsynaptic sites. J Neurosci. 2014;34:15415-24 pubmed publisher
Bailey E, Green S. Postnatal expression of neurotrophic factors accessible to spiral ganglion neurons in the auditory system of adult hearing and deafened rats. J Neurosci. 2014;34:13110-26 pubmed publisher
Kang Y, Ge Y, Cassidy R, Lam V, Luo L, Moon K, et al. A combined transgenic proteomic analysis and regulated trafficking of neuroligin-2. J Biol Chem. 2014;289:29350-64 pubmed publisher
Frandemiche M, De Seranno S, Rush T, Borel E, Elie A, Arnal I, et al. Activity-dependent tau protein translocation to excitatory synapse is disrupted by exposure to amyloid-beta oligomers. J Neurosci. 2014;34:6084-97 pubmed publisher
Ting J, Marks D, Schleidt S, Wu J, Zyskind J, Lindl K, et al. Targeted gene mutation of E2F1 evokes age-dependent synaptic disruption and behavioral deficits. J Neurochem. 2014;129:850-63 pubmed publisher
Shi L, Liu L, He T, Guo X, Yu Z, Yin S, et al. Ribbon synapse plasticity in the cochleae of Guinea pigs after noise-induced silent damage. PLoS ONE. 2013;8:e81566 pubmed publisher
Tabatabaei M, Caetano F, Vedraine S, Norton P, Ferguson S, Lagugné Labarthet F. Directing GPCR-transfected cells and neuronal projections with nano-scale resolution. Biomaterials. 2013;34:10065-74 pubmed publisher
Nakajima C, Kulik A, Frotscher M, Herz J, Schafer M, Bock H, et al. Low density lipoprotein receptor-related protein 1 (LRP1) modulates N-methyl-D-aspartate (NMDA) receptor-dependent intracellular signaling and NMDA-induced regulation of postsynaptic protein complexes. J Biol Chem. 2013;288:21909-23 pubmed publisher
Nayagam B, Edge A, Needham K, Hyakumura T, Leung J, Nayagam D, et al. An in vitro model of developmental synaptogenesis using cocultures of human neural progenitors and cochlear explants. Stem Cells Dev. 2013;22:901-12 pubmed publisher
Lemieux M, Labrecque S, Tardif C, Labrie Dion É, Lebel E, De Koninck P. Translocation of CaMKII to dendritic microtubules supports the plasticity of local synapses. J Cell Biol. 2012;198:1055-73 pubmed publisher
Wang Y, Wang J, Wang S, Liu S, Cao J, Li X, et al. Adaptor protein APPL1 couples synaptic NMDA receptor with neuronal prosurvival phosphatidylinositol 3-kinase/Akt pathway. J Neurosci. 2012;32:11919-29 pubmed publisher
Henson M, Larsen R, Lawson S, Pérez Otaño I, Nakanishi N, Lipton S, et al. Genetic deletion of NR3A accelerates glutamatergic synapse maturation. PLoS ONE. 2012;7:e42327 pubmed publisher
Mandemakers W, Snellinx A, O Neill M, De Strooper B. LRRK2 expression is enriched in the striosomal compartment of mouse striatum. Neurobiol Dis. 2012;48:582-93 pubmed publisher
Teo L, Homman Ludiye J, Rodger J, Bourne J. Discrete ephrin-B1 expression by specific layers of the primate retinogeniculostriate system continues throughout postnatal and adult life. J Comp Neurol. 2012;520:2941-56 pubmed publisher
She K, Ferreira J, Carvalho A, Craig A. Glutamate binding to the GluN2B subunit controls surface trafficking of N-methyl-D-aspartate (NMDA) receptors. J Biol Chem. 2012;287:27432-45 pubmed publisher
Stansfield K, Pilsner J, Lu Q, Wright R, Guilarte T. Dysregulation of BDNF-TrkB signaling in developing hippocampal neurons by Pb(2+): implications for an environmental basis of neurodevelopmental disorders. Toxicol Sci. 2012;127:277-95 pubmed publisher
He P, Liu Q, Wu J, Shen Y. Genetic deletion of TNF receptor suppresses excitatory synaptic transmission via reducing AMPA receptor synaptic localization in cortical neurons. FASEB J. 2012;26:334-45 pubmed publisher
She K, Craig A. NMDA receptors mediate synaptic competition in culture. PLoS ONE. 2011;6:e24423 pubmed publisher
Neal A, Worley P, Guilarte T. Lead exposure during synaptogenesis alters NMDA receptor targeting via NMDA receptor inhibition. Neurotoxicology. 2011;32:281-9 pubmed publisher
Jacobs S, Nathwani M, Doering L. Fragile X astrocytes induce developmental delays in dendrite maturation and synaptic protein expression. BMC Neurosci. 2010;11:132 pubmed publisher
Gil Bea F, Solas M, Mateos L, Winblad B, Ramirez M, Cedazo Minguez A. Cholinergic hypofunction impairs memory acquisition possibly through hippocampal Arc and BDNF downregulation. Hippocampus. 2011;21:999-1009 pubmed publisher
Zhang D, Zhang J, Bian C, Deng Q. Postnatal and ovariectomic regulation of postsynaptic density protein-95 in the hippocampus of female Sprague-Dawley rats. Synapse. 2010;64:875-8 pubmed publisher
Li Y, Serwanski D, Miralles C, Fiondella C, LoTurco J, Rubio M, et al. Synaptic and nonsynaptic localization of protocadherin-gammaC5 in the rat brain. J Comp Neurol. 2010;518:3439-63 pubmed publisher
Siddiqui T, Pancaroglu R, Kang Y, Rooyakkers A, Craig A. LRRTMs and neuroligins bind neurexins with a differential code to cooperate in glutamate synapse development. J Neurosci. 2010;30:7495-506 pubmed publisher
McDowell K, Hutchinson A, Wong Goodrich S, Presby M, Su D, Rodriguiz R, et al. Reduced cortical BDNF expression and aberrant memory in Carf knock-out mice. J Neurosci. 2010;30:7453-65 pubmed publisher
Jacobs S, Doering L. Astrocytes prevent abnormal neuronal development in the fragile x mouse. J Neurosci. 2010;30:4508-14 pubmed publisher
Marks D, Tucker K, Cavallin M, Mast T, Fadool D. Awake intranasal insulin delivery modifies protein complexes and alters memory, anxiety, and olfactory behaviors. J Neurosci. 2009;29:6734-51 pubmed publisher
Feyissa A, Chandran A, Stockmeier C, Karolewicz B. Reduced levels of NR2A and NR2B subunits of NMDA receptor and PSD-95 in the prefrontal cortex in major depression. Prog Neuropsychopharmacol Biol Psychiatry. 2009;33:70-5 pubmed publisher
Cheng H, Huang Z, Lin W, Chow W, Chang Y. Cold-induced exodus of postsynaptic proteins from dendritic spines. J Neurosci Res. 2009;87:460-9 pubmed publisher
VanGuilder H, Brucklacher R, Patel K, Ellis R, Freeman W, Barber A. Diabetes downregulates presynaptic proteins and reduces basal synapsin I phosphorylation in rat retina. Eur J Neurosci. 2008;28:1-11 pubmed publisher
Roberts T, Klein M, Kubke M, Wild J, Mooney R. Telencephalic neurons monosynaptically link brainstem and forebrain premotor networks necessary for song. J Neurosci. 2008;28:3479-89 pubmed publisher
Junghans D, Heidenreich M, Hack I, Taylor V, Frotscher M, Kemler R. Postsynaptic and differential localization to neuronal subtypes of protocadherin beta16 in the mammalian central nervous system. Eur J Neurosci. 2008;27:559-71 pubmed publisher
CARLSON E, Stead J, Neal C, Petryk A, Georgieff M. Perinatal iron deficiency results in altered developmental expression of genes mediating energy metabolism and neuronal morphogenesis in hippocampus. Hippocampus. 2007;17:679-91 pubmed
Guo L, Wang Y. Glutamate stimulates glutamate receptor interacting protein 1 degradation by ubiquitin-proteasome system to regulate surface expression of GluR2. Neuroscience. 2007;145:100-9 pubmed
Yashiro K, Corlew R, Philpot B. Visual deprivation modifies both presynaptic glutamate release and the composition of perisynaptic/extrasynaptic NMDA receptors in adult visual cortex. J Neurosci. 2005;25:11684-92 pubmed
product information
Catalog Number :
MAB1596
Subcategory :
Neuroscience
Product Name :
Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P78352
Host Name :
Mouse
Antigen :
Post Synaptic Density Protein 95
Clone :
6G6-1C9
Conjugate :
Purified
Isotype :
IgG2a
Product Description :
Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9
Cross Reactivity :
Bovine;Mouse;Rat
Background :
PSD-95 is part of a multiprotein complex restricted to the post-synaptic membrane. It is believed to initiate NMDA receptor clustering on the post synaptic membrane. Its restricted expression makes it an excellent marker for post-synaptic membrane regions on neurons.
ALT Names :
PSD-95
Immunogen :
Recombinant rat PSD-95.
Specificity :
Reacts with a protein of ~100 kDa, corresponding to the apparent molecular weight of PSD-95, on SDS-PAGE immunoblots of samples from rat, mouse and bovine. A slightly higher molecular weight band is also detected. In rat brain and mouse brain tissue extracts a band of ~75 kDa is also present.
Package Size :
100 µg
Uses :
Immunocytochemistry;Immunohistochemistry;Western Blotting
Storage :
Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Do not store below -20°C. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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